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Multispan, Inc.

Hayward, California, US

Multispan, Inc. is a premier assay screening technology provider focusing primarily on cellular drug discovery research targeting GPCR, RTK, NHR, Transporter, Integrin, AMPK, DNA-PK and beyond. It has developed proprietary HExTM expression technology and created the industry’s largest, highly characterized, high quality GPCR-expressing stable cell lines. The assay platform enables HTS and lead optimization applications in new drug discovery programs to treat cardiovascular, metabolic, neurodegenerative, depression, inflammation, immunology and oncology diseases.

Multispan’s high standards of quality and integrity has earned many successful collaborations with a wide range of leading pharmaceutical and biotech companies globally. Our goal is not to add another project; our goal is to help you succeed.

Multispan, Inc. has not listed any services.

Stable Cell Line Generation
Price on request

Stable cell lines are among the best tools for screening bioactive compounds and studying MOA such as all signaling-biases in one cell line. We have developed a robust platform to produce and select single cell clones and a strong track record in 100% successful delivery.
• Minimally modified and hold to the industry’s highest... Show more »

Stable cell lines are among the best tools for screening bioactive compounds and studying MOA such as all signaling-biases in one cell line. We have developed a robust platform to produce and select single cell clones and a strong track record in 100% successful delivery.
• Minimally modified and hold to the industry’s highest quality standards
• Directly screened in relevant functional assays from a plateful of single cell clones
• Specialize in difficult targets, partial agonism vs full agonism, and allosteric vs orthosteric binders, comparable ortholog and human assays
• 99% of the stable cell lines pass rigorous 2-month stability quality control testing

Adenosine A1, A2A, A2B, A3; Adhesion GPR97; Adrenergic a1A, a1B, a1D, a2A, a2B, a2C, b1, b2, b3; Anaphylatoxin C3AR1, C5R1; Angiotensin AT1; Apelin APJ; Bile Acid GPBA; Bombesin BB1, BB2, BB3; Bradykinin B1, B2; Calcitonin CALCRL, CT; Calcium-Sensing CaSR; Cannabinoid CB1, CB2, GPR55; Chemokine CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CCR10, CMKLR1, CX3CR1, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6, CXCR7, XCR1; Cholecystokinin CCK1, CCK2; Citric Acid Cycle Intermediates GPR91, GPR99; Corticotrophin-Releasing Factor CRF1, CRF2; Dopamine D1, D2, D4, D5; Endothelin ETA, ETB; Free Fatty Acid GPR119, GPR120, GPR40, GPR41, GPR43, GPR84; Frizzled SMO; Galanin GAL1, GAL2; Ghrelin, Glucagon, GNRH, GIP, GLP-1, GLP-2, Secretin; Glycoprotein Hormone TSH; Gonadotrophin-Releasing Hormone GnRH; Histamine H1, H2, H3, H4; Hydrocarboxylic Acid GPR81; KiSS1-Derived Peptide KiSS1; Leukotriene/Lipoxin ALX, BLT1, BLT2, CysLT1, CysLT2, OXE; Lysophospholipid LPA1, LPA2, LPA3, LPA4, LPA5, S1P1, S1P2, S1P3, S1P4, S1P5; Melanin-Concentrating Hormone MCH1, MCH2; Melanocortin MC1, MC4; Melatonin MT1, MT2; Metabotropic Glutamate mGlu1, mGlu2, mGlu3, mGlu4, mGlu5, mGlu6, mGlu7, mGlu8; Motilin; Muscarinic M1, M2, M3, M4, M5; N-formylpeptide FPR1; Neuromedin U NMU1, NMU2; Neuropeptide FF/AF NPFF1, NPFF2, NPS, NPS(2), NPSlle107, NPBW1, NPBW2, Y1, Y2, Y4, Y5; Neurotensin NTS1, NTS2; Nicotinic Acid GPR109A, GPR109B; Opioid Delta, Kappa, mu, NOP; Orexin OX1, OX2; Orphan GPR3, GPR35, GPR50, GPR142, MRGX1, MRGX2; Oxysterol EBI2; Parathyroid Hormone PTH1, PTH2; Platelet-Activating Factor PAF; Prokineticin PK1, PK2, PK2 mutant; Prolactin-Releasing Peptide PrRP; Prostanoid DP1, DP2, EP1, EP2, EP3, EP4, FP, IP1, TP; Protease-Activated PAR1, PAR2, PAR4; Purinergic GPR17, P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, P2Y14; Relaxin RXFP1, RXFP2, RXFP3; Serotonin 5-HT1A, 5-HT1B, 5-HT2A, 5-HT2B, 5-HT2C, 5-HT4, 5-HT6, 5-HT7; Somatostatin sst1, sst2, sst3, sst4, sst5; Tachykinin NK1, NK2, NK3; Thyrotropin-Releasing Hormone TRH; Trace Amine TA1; Urotensin UT; Vasoactive Intestinal Peptide PAC1, VPAC1, VPAC2; Vasopressin/Oxytocin OT, V1A, V1B, V2

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Human
Rabbit
Guinea pig
Mouse
Rat
Plate reader
Flow Cytometry
CHO-K1
HEK293T
RH7777
Stable Cell Line
HEK293R
C6
Dog
MIN6
HeLa
1321N1
Oncology
Drug discovery
CNS/Neurology
Pain
cardiovascular
Immunooncology
Inflammation
GPCR
Orphan Disease
Allergy
Metabolic Disease
Transporter
Nuclear Hormone Receptor
kinase
Non-human primates
FLIPR
Custom Cell-Based Assays
Price on request

Let Multispan develop relevant cellular and biochemical assays and conduct screens to reduce your cycle time to IND or biologics product release QC. We work with you from design to finish to give you a consistent, robust and physiologically relevant assay you need.

• GPCRs, kinases and beyond.
• Second messenger, reporter,... Show more »

Let Multispan develop relevant cellular and biochemical assays and conduct screens to reduce your cycle time to IND or biologics product release QC. We work with you from design to finish to give you a consistent, robust and physiologically relevant assay you need.

• GPCRs, kinases and beyond.
• Second messenger, reporter, and phenotypic assays in cellular, ELISA or other biochemical format.
• All GPCR assay technologies and beyond in hand for Calcium, cAMP, arrestin, pGRK2, pAMPK, pDNA-PK, pERK, IP-1, pNFkB, radioligand binding, GTPgS, chemotaxis, cell proliferation, cytokine secretion, insulin secretion and internalization assays.
• In our MultiscreenTM recombinant stable cell lines or primary cells.
• Agonist, inverse agonist, antagonist, PAM or NAM modes.
• Developed over 50 ortholog GPCRs for counter screening including rodent, canine, and non-human primate

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Calcium
Dog
GPCR Assay
Internalization Assay
pGRK2
IP-1
cAMP
pERK
pNFkB
Radioligand Binding
GTPgS
Chemotaxis
Insulin Secretion
Rodents
GPCR
Transporter
Nuclear Hormone Receptor
kinase
Non-human primates
arrestin
pDNA-PK
pAMPK
cytokine secretion
EROD
luciferase
ELISA
TR-FRET
Internalization
Proliferation
2nd messenger
Phenotypic assay
GPCR Screening and Profiling
Price on request

Shorten time to results by letting Multispan’s assay experts screen compounds directly in assay developed in house.
• Save a few months in assay transfer
• Quick turnaround: 1-2 days for SAR, 1-2 weeks for profiling, 1 month for HTS
• High Quality: Uncompromising quality results and assay consistency by industry standards... Show more »

Shorten time to results by letting Multispan’s assay experts screen compounds directly in assay developed in house.
• Save a few months in assay transfer
• Quick turnaround: 1-2 days for SAR, 1-2 weeks for profiling, 1 month for HTS
• High Quality: Uncompromising quality results and assay consistency by industry standards
• Relevant signaling, binding or phenotypic assays in MultiScreenTM recombinant or endogenous primary cells.
• Enriched active assay platform for GPCRs, kinases and beyond: cAMP, arrestin, pGRK2, pAMPK, pDNA-PK, pERK, IP-1, pNFkB, radioligand binding, GTPgS, chemotaxis, cell proliferation, cytokine secretion, insulin secretion and internalization assays
• Proven track record: HTS, hit-to-lead, primary and secondary lead optimization screening support
• High value results: Human and ortholog GPCRs and beyond in agonist, inverse agonist, antagonist, PAM and NAM modes.
• Flexibility in panel size: Customizable 470 validated assays for 270 targets and custom matching orthologs
• 32-GPCR safety/liability panel implicated in central nervous, cardiac, pulmonary and GI concerns.
• 75-oprhan GPCR Calcium assay panel - known GPCRs and FACS as controls.

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Human
Mouse
Rat
GraphPad Prism
Molecular Devices Softmax Pro
Molecular Devices FLIPR
Molecular Devices Flexstation 3
BD FACSort
Dog
Drug discovery
Non-human primates
Lead optimization, HTS
Cell Culture Scale-Up
Price on request

Replacing laborious cell culture required by HTS, lead optimization, and GMP product batch release QC

b. Deliverables: Functionally validated cells ready to use
c. Cell Lines: Dividing stable cell lines purchased from Multispan or third party.
d. Grown from master cell bank with lowest passage number.
e. Scaled up to... Show more »

Replacing laborious cell culture required by HTS, lead optimization, and GMP product batch release QC

b. Deliverables: Functionally validated cells ready to use
c. Cell Lines: Dividing stable cell lines purchased from Multispan or third party.
d. Grown from master cell bank with lowest passage number.
e. Scaled up to 0.5-10 billion cells and packaged in 10-50 million cells per frozen vial.

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Drug discovery
tissue culture
Division Arrested Cell Lines
Price on request

Division-arrested cells offer convenient tools for cell-based high-throughput screening
• Comparable EC50 values have been achieved from division-arrested and dividing cells, demonstrating excellent pharmacological performance of the receptors used.
• Successfully prepared for different assay formats from various parental cells... Show more »

Division-arrested cells offer convenient tools for cell-based high-throughput screening
• Comparable EC50 values have been achieved from division-arrested and dividing cells, demonstrating excellent pharmacological performance of the receptors used.
• Successfully prepared for different assay formats from various parental cells expressing GPCRs stably or transiently.
• Offered for screening, profiling and other applications.
• Plate and read with ready-to-go assay conditions
• Low-cost evaluation before purchasing a stable cell line
• Low-cost alternative for analyzing limited number of compounds
• Two week turnaround for rapid delivery
• Quality control is performed by each lot for functionality
• Derived from well characterized stable cell lines
**Please refer to stable cell line for full list of receptors

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Human
Rabbit
Guinea pig
Mouse
Rat
CHO-K1
HEK293T
GPCR
Dog
Division Arrested Cells
Drug discovery
Non-human primates
Membrane Protein Expression
Price on request

Membranes preparations are ideal for binding assays.
• High receptor density
• Prepared from Multispan’s stable cell lines for minimal lot-to-lot variability
• Two week turnaround for rapid delivery
• Quality control by FACs for surface expression
• Derived from well characterized stable cell lines
**Please refer to stable cell line for full list of receptors

Membranes preparations are ideal for binding assays.
• High receptor density
• Prepared from Multispan’s stable cell lines for minimal lot-to-lot variability
• Two week turnaround for rapid delivery
• Quality control by FACs for surface expression
• Derived from well characterized stable cell lines
**Please refer to stable cell line for full list of receptors

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Human
Rabbit
Guinea pig
Mouse
Rat
CHO-K1
HEK293T
GPCR
Dog
Membrane preparation
Membrane protein
Drug discovery
Non-human primates
Custom Projects
Price on request

Ask us about your favorite GPCR drug targets! We can screen your compounds or enable you to do it.
• Signaling bias pharmacology all in ONE cell line: Using minimally modified GPCRs in MultiScreenTM recombinant stable cell lines or endogenously expressed in primary cells.
• Monthly receptor isotype compound profiling programs:... Show more »

Ask us about your favorite GPCR drug targets! We can screen your compounds or enable you to do it.
• Signaling bias pharmacology all in ONE cell line: Using minimally modified GPCRs in MultiScreenTM recombinant stable cell lines or endogenously expressed in primary cells.
• Monthly receptor isotype compound profiling programs: to guide SAR campaigns in related complete receptor families such as mGlu, FFA, LPA, S1P, opioid, and chemokine.
• Assay Quality Requirements for SAR Screening: 2 sets of independent data back-to-back for each test including an internal proprietary control. Any questionable data will be repeated for free automatically.

Multispan provides custom-made GPCR stable cell lines upon request to meet your specific needs in GPCR high throughput screening and compound profiling.
• Two-month turnaround
• Most relevant biological readouts with upfront input directly from customers
• Optimal signal-to-noise ratio for high throughput screening
• Pharmacological consistency with literature data

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GPCR
High Throughput Screening
Compound Profiling
mGlu
FFA
LPA
S1P
Opioid
Chemokine
Monoclonal Antibody (mAb) Profiling
Price on request

Issue: There are 350 "druggable" GPCRs which share many similar primary and conformational epitopes. Is your antibody lead SPECIFIC to your GPCR target of interest?

Solution: Assess specificity of the antibody lead in Multispan's large GPCR expression panel. We measure your antibody binding to GPCRs in NATIVE... Show more »

Issue: There are 350 "druggable" GPCRs which share many similar primary and conformational epitopes. Is your antibody lead SPECIFIC to your GPCR target of interest?

Solution: Assess specificity of the antibody lead in Multispan's large GPCR expression panel. We measure your antibody binding to GPCRs in NATIVE conformations directly on mammalian cell surface by FACS.

Benefits: Obtain valuable information to

  • make Go-Or-No-Go decision early
  • save cost in expensive animal and clinical studies
  • shorten lead development cycle

Convenience: Send us your purified, un-labeled antibody and you will have the results back in 1 week.

Flexibility: Choose your own panel from our 320 human and 180 murine full-length cDNAs in Multispan's powerful proprietary expression vectors.

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Receptor Deorphanization
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Monoclonal Antibody (mAb) Production
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Luciferase Assays
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High Throughput Screening
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In vitro Insulin Secretion Assay
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Integrated Drug Discovery
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Flow Cytometry
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Assay Development
Price on request

Custom assay development for GPCRs, kinase receptors, endogenously expressed receptors in primary cells, and other transmembrane proteins

We provide custom assay development services for drug discovery worldwide to biotechnology companies, pharmaceutical companies and academic institutions. Whether you need assays for screening... Show more »

Custom assay development for GPCRs, kinase receptors, endogenously expressed receptors in primary cells, and other transmembrane proteins

We provide custom assay development services for drug discovery worldwide to biotechnology companies, pharmaceutical companies and academic institutions. Whether you need assays for screening or GMP biologics product batch release QC, Multispan’s scientists have extensive experience to help accelerate your research by providing comprehensive GPCR cell biology solutions using the most cutting edge assay technologies.

  1. Customized cell-based, phenotypic and binding assays for your specific research or discovery target of interest in ONE cell line with Multispan's minimally modified GPCRs in recombinant stable cell lines, endogenously expressed in primary cells, and kinase stable cell lines.
  2. Variety of different custom assay formats using detection kits commercially available from Multispan and others, or using custom-assembled reagents to achieve the best results.
    • Calcium
    • cAMP
    • Chemotaxis
    • Internalization
    • Insulin secretion
    • IP-1
    • Luciferase reporter gene assay
    • pERK
    • pGRK-2
    • pNFkB
    • Radioligand binding
    • GTPgS
  3. Services include design, assay development, assay optimization, assay validation and method documentation
  4. Agonist, antagonist, PAM and NAM modes
  5. Complete research confidentiality assured
  6. Proprietary technologies and extensive experience guarantee success and 100% delivery track record
  7. Rapid 1-month turnaround time with comprehensive report
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Compound Profiling
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Protein Characterization Services
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Lead Identification and Validation
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Protein Expression
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Mammalian Cell Culture
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Biochemistry & Molecular Biology
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Drug Discovery & Development
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In vitro Reporter Gene Assays
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Biochemical Assays
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Receptor Assays
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Experimental Design
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Hit Identification
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Protein Services
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Cell-Based Screening Methods
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Project Management & Consulting Services
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Functional & Cell Type Specific Assays
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Cell-Based Assays
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Biology
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Antibody Services
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Cytometry
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Experiment Outsourcing
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Method development
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FACS
Fluorescence Activated Cell Sorting
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Cell and Tissue Culture
Price on request

Cell and Tissue Culture Services

Cell and Tissue Culture Services

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2017-10-23 00:54:23 -0700

Net Promoter Score of 10 received for Stable Cell Line Generation.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.

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