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Multispan, Inc.

9 Orders Completed
Hayward, California, US

About Multispan, Inc.

Multispan, Inc. is a premier cell engineering and assay screening technology provider focusing on drug discovery for new therapeutics targeting GPCRs and beyond. We have developed proprietary cellular expression and MultiScreen™ Beta-Arrestin Sensor technologies, as well as the industry’s largest, highly characterized native GPCR-expressing stable cell lines for HTS and lead optimization. Our uncompromising high-quality and integrity have earned us many successful long-term pharmaceutical and biotech collaborations globally. We are here to help you succeed.

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Minority owned business Woman owned small business

Our Services (11)


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Cell-Based Compound Screening

Price on request

**Screening and Profiling (including GPCR screening and profiling)** Multispan’s assay experts screen compounds directly in cell-based and biochemical assays developed in house; save a few months in assay transfer and shorten time to results. • GPCR, kinase, NHR, receptor tyrosine kinase and beyond. • Quick turnaround: 1-2 days for SAR, 1-2 weeks for profiling, 1 month for HTS • Relevant signaling, binding or phenotypic assays in MultiScreenTM stable or primary cells. • MultiScreen arrestin sensor, internalization, Ca++, cAMP, K+, EROD, reporter, pGRK2, pAMPK, pDNA-PK, pERK, IP-1, pNFkB, radioligand binding, GTPgS, chemotaxis, cell proliferation, cytokine secretion, insulin. • Proven track record: HTS, hit-to-lead, primary and secondary lead optimization screening support • Agonist, inverse agonist, antagonist, PAM and NAM modes. • Customizable 470 validated assays for 270 targets and custom matching orthologs • 32-GPCR safety/liability panel implicated in central nervous, cardiac, pulmonary and GI concerns. • 75-oprhan GPCR Calcium assay panel • Signaling GPCR bias pharmacology all in ONE cell line • Uncompromising quality results and assay consistency

Drug Discovery CRISPR Cas9 Crispr GPCR Ion Channel kinase Transporter Show 7 more tags Show less

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Cell Line Generation

Price on request

Robust platform to produce and select single cell clones; over-expression or CRISPR knock-out, a strong track record in 100% successful Delivery; best tools for screening bioactive compounds and studying MOA like “all signaling-biases in one cell line” • GPCRs, NHR, integrin, kinases, receptor tyrosine kinases, ion channel, transporter • Minimally modified and hold to the industry’s highest quality standards • Directly screened in relevant functional assays from a plateful of single cell clones • Specialize in 1) difficult targets, 2) partial agonism vs full agonism, 3) andallosteric vs orthosteric binders, and 4) comparable ortholog and human assays • 99% of the stable cell lines pass rigorous 2-month stability quality control testing

Human Mouse Rat Dog Guinea pig Rabbit Non-human primates (NHP) FLIPR Microplate Reader Flow cytometry Drug Discovery kinase Transporter Nuclear Hormone Receptor Pain Inflammation Oncology CNS/Neurology Cardiovascular Metabolism and Endocrinology Immunology Rare Disease GPCR Ion Channel Cas9 Crispr CRISPR Cas9 Crispr System Show 27 more tags Show less

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Assay Development

Price on request

Custom assay development; extensive experience; shorten time to IND and biologics GMP product release QC; 100% successful delivery track record.

• GPCR, kinase, NHR and beyond
• Endogenously expressed targets in primary cells or MultiScreen stable cell lines
• 384-well biochemical or cell based format: 2nd messenger, reporter, ELISA, TR-FRET, or phenotypic assays.
• MultiScreen arrestin sensor, internalization, Ca++, K+, cAMP, pGRK2, pAMPK, pDNA-PK, reporter, EROD, pERK, IP-1, pNFkB, radioligand binding, GTPgS, chemotaxis, cell proliferation, cytokine secretion, insulin.
• In our MultiscreenTM recombinant stable cell lines or primary cells.
• Developed over 50 ortholog GPCR assays including rodent, canine, and non-human primate.


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Cell Culture Scale-Up

Price on request

Replacing laborious cell culture required by HTS, lead optimization, and GMP product batch release QC

b. Deliverables: Functionally validated cells ready to use
c. Cell Lines: Dividing stable cell lines purchased from Multispan or third party.
d. Grown from master cell bank with lowest passage number.
e. Scaled up to 0.5-10 billion cells and packaged in 10-50 million cells per frozen vial.

tissue culture Drug Discovery

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Division Arrested Cell Lines

Price on request

Division-arrested cells offer convenient tools for cell-based high-throughput screening
• Comparable EC50 values have been achieved from division-arrested and dividing cells, demonstrating excellent pharmacological performance of the receptors used.
• Successfully prepared for different assay formats from various parental cells expressing GPCRs stably or transiently.
• Offered for screening, profiling and other applications.
• Plate and read with ready-to-go assay conditions
• Low-cost evaluation before purchasing a stable cell line
• Low-cost alternative for analyzing limited number of compounds
• Two week turnaround for rapid delivery
• Quality control is performed by each lot for functionality
• Derived from well characterized stable cell lines
**Please refer to stable cell line for full list of receptors

Human Rat Mouse Dog Guinea pig Rabbit Non-human primates (NHP) Drug Discovery Show 8 more tags Show less

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Membrane Protein Expression

Price on request

Membranes preparations are ideal for binding assays.
• High receptor density
• Prepared from Multispan’s stable cell lines for minimal lot-to-lot variability
• Two week turnaround for rapid delivery
• Quality control by FACs for surface expression
• Derived from well characterized stable cell lines
**Please refer to stable cell line for full list of receptors

Human Rat Mouse Dog Guinea pig Rabbit Non-human primates (NHP) Drug Discovery Show 8 more tags Show less

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GPCR Assays

G-Protein Coupled Receptor Assay
Price on request

Ask us about your favorite GPCR drug targets! We can screen your compounds or enable you to do it.
• Signaling bias pharmacology all in ONE cell line: Using minimally modified GPCRs in MultiScreenTM recombinant stable cell lines or endogenously expressed in primary cells.
• Monthly receptor isotype compound profiling programs: to guide SAR campaigns in related complete receptor families such as mGlu, FFA, LPA, S1P, opioid, and chemokine.
• Assay Quality Requirements for SAR Screening: 2 sets of independent data back-to-back for each test including an internal proprietary control. Any questionable data will be repeated for free automatically.

Multispan provides custom-made GPCR stable cell lines upon request to meet your specific needs in GPCR high throughput screening and compound profiling.
• Two-month turnaround
• Most relevant biological readouts with upfront input directly from customers
• Optimal signal-to-noise ratio for high throughput screening
• Pharmacological consistency with literature data

High Throughput Screening

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Monoclonal Antibody (mAb) Profiling

Price on request

Issue: There are 350 "druggable" GPCRs which share many similar primary and conformational epitopes. Is your antibody lead SPECIFIC to your GPCR target of interest?

Solution: Assess specificity of the antibody lead in Multispan's large GPCR expression panel. We measure your antibody binding to GPCRs in NATIVE conformations directly on mammalian cell surface by FACS.

Benefits: Obtain valuable information to

  • make Go-Or-No-Go decision early
  • save cost in expensive animal and clinical studies
  • shorten lead development cycle

Convenience: Send us your purified, un-labeled antibody and you will have the results back in 1 week.

Flexibility: Choose your own panel from our 320 human and 180 murine full-length cDNAs in Multispan's powerful proprietary expression vectors.


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Compound Profiling

Price on request

Screening and Profiling (including GPCR screening and profiling)

Multispan’s assay experts screen compounds directly in assay developed in house; save a few months in assay transfer and shorten time to results.
• GPCR, kinase, NHR, receptor tyrosine kinase and beyond.
• Quick turnaround: 1-2 days for SAR, 1-2 weeks for profiling, 1 month for HTS
• Relevant signaling, binding or phenotypic assays in MultiScreenTM stable or primary cells.
• MultiScreen arrestin sensor, internalization, Ca++, cAMP, K+, EROD, reporter, pGRK2, pAMPK, pDNA-PK, pERK, IP-1, pNFkB, radioligand binding, GTPgS, chemotaxis, cell proliferation, cytokine secretion, insulin.
• Proven track record: HTS, hit-to-lead, primary and secondary lead optimization screening support
• Agonist, inverse agonist, antagonist, PAM and NAM modes.
• Customizable 470 validated assays for 270 targets and custom matching orthologs
• 32-GPCR safety/liability panel implicated in central nervous, cardiac, pulmonary and GI concerns.
• 75-oprhan GPCR Calcium assay panel
• Signaling GPCR bias pharmacology all in ONE cell line
• Uncompromising quality results and assay consistency


ic

GPCR Screening and Profiling

Price on request

Screening and Profiling (including GPCR screening and profiling)

Multispan’s assay experts screen compounds directly in assay developed in house; save a few months in assay transfer and shorten time to results.
• GPCR, kinase, NHR, receptor tyrosine kinase and beyond.
• Quick turnaround: 1-2 days for SAR, 1-2 weeks for profiling, 1 month for HTS
• Relevant signaling, binding or phenotypic assays in MultiScreenTM stable or primary cells.
• MultiScreen arrestin sensor, internalization, Ca++, cAMP, K+, EROD, reporter, pGRK2, pAMPK, pDNA-PK, pERK, IP-1, pNFkB, radioligand binding, GTPgS, chemotaxis, cell proliferation, cytokine secretion, insulin.
• Proven track record: HTS, hit-to-lead, primary and secondary lead optimization screening support
• Agonist, inverse agonist, antagonist, PAM and NAM modes.
• Customizable 470 validated assays for 270 targets and custom matching orthologs
• 32-GPCR safety/liability panel implicated in central nervous, cardiac, pulmonary and GI concerns.
• 75-oprhan GPCR Calcium assay panel
• Signaling GPCR bias pharmacology all in ONE cell line
• Uncompromising quality results and assay consistency

MultiScreen b-Arrestin Sensor

MultiScreen b-Arrestin Sensor for Native GPCRs
• True phamacology in vitro and in vivo without GPCR tagging
• Study all signaling bias in primary cells or one MultiScreen stable cell line
• No GRK2 co-transfection required
• We screen your compounds or support you to set assays up for HTS or SAR

Molecular Devices FLIPR Molecular Devices Flexstation 3 BD FACSort Molecular Devices Softmax Pro GraphPad Prism Human Mouse Rat Dog Non-human primates (NHP) Drug Discovery Show 11 more tags Show less

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Custom Cell-Based Assay Development

Price on request

Custom development of relevant cellular and biochemical assays; reduce cycle time to IND or biologics release QC; extensive experience, consultation from design to finish; 100% successful delivery track record

• GPCR, kinase, NHR and beyond
• Endogenously expressed targets in primary cells or MultiScreen stable cell lines
• 384-well 2nd messenger, reporter, phenotypic assays in cellular, ELISA or other format.
• MultiScreen arrestin sensor, internalization, Ca++, K+, cAMP, pGRK2, pMAPK, pDNA-PK, pERK, IP-1, pNFkB, radioligand binding, GTPgS, chemotaxis, cell proliferation, cytokine secretion, insulin.
• In our MultiscreenTM recombinant stable cell lines or primary cells.
• Developed over 50 ortholog GPCR assays including rodent, canine, and non-human primate.

Calcium pGRK2 IP-1 cAMP pERK pNFkB Radioligand Binding GTPgS Chemotaxis Insulin Secretion Rodents Non-human primates (NHP) Dog arrestin pDNA-PK pAMPK cytokine secretion EROD luciferase ELISA TR-FRET Internalization Proliferation Phenotypic screening GPCR kinase Nuclear Hormone Receptor Transporter Show 28 more tags Show less

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JP

Jennifer Pham

Associate Scientist
PY

Patricia Yeung

Research Assistant

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