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MS Bioworks

Ann Arbor, Michigan, US

MS Bioworks is a state of the art protein mass spectrometry service provider delivering the highest quality data to Industrial and government organizations and academic institutions. We believe in developing long term relationships with our clients and partnering for our mutual success. Whether you are trying to identify a protein, discover new biomarkers or characterize the glycoforms of a therapeutic protein let us be your partner in innovation.

MS Bioworks has not listed any services.

Protein Identification
Starting at $250.00 per sample

These services enable the identification of proteins in a gel band, gel spot or in solution. If you would like to identify a protein in a gel band or gel spot you should select our MSB-01 service. If you would like to identify a protein in solution you should select our MSB-02 service.

MSB-01 can be used to identify proteins in... Show more »

These services enable the identification of proteins in a gel band, gel spot or in solution. If you would like to identify a protein in a gel band or gel spot you should select our MSB-01 service. If you would like to identify a protein in solution you should select our MSB-02 service.

MSB-01 can be used to identify proteins in gel bands or gel spots. We can accept any type of gel matrix and most stains. If you are using a silver staining, please avoid the Bio-Rad Silver Stain Plus Kit. For the greatest sensitivity towards your target you should cut the band close to your target, the gel band dimensions will be ~5mm x 2mm. We can get an identification with as little as 1ng of protein on gel. There is a link at the bottom of the page that will let you view a Service Note that provides a useful sensitivity guide.

MSB-02 can be used to identify a protein in solution. We recommend submitting samples at a protein concentration of ≥1µg/µL in 10µL of buffer. This is just a guide, if you any concerns about the suitability of this service for the analysis of your samples please call or email. If you provide detailed buffer information with your samples it can be helpful. If not addressed during processing buffers containing detergents and non-volatile salts can interfere with the analysis.

Steps Involved
Sample preparation: In-gel digestion with trypsin using a ProGest (Digilab) robot. Solution digestion with trypsin is performed manually.
LC-MS/MS: Digested samples are analyzed with 0.5hr LC-MS/MS
Data analysis: Protein identification is performed with Mascot. Protein visualization and validation is performed with Scaffold.
Report generation: PDF/Excel/Scaffold
The turnaround time is 2-3 weeks.

Deliverables
Written Report containing details of your samples, our methods and a summary of the data.
An Excel workbook containing the detailed qualitative and quantitative protein identification data.
A Scaffold file to enable your own deeper data mining.
An example report is available upon request.

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Intact Mass Analysis
Starting at $390.00 per sample

Overview

An intact mass measurement can be used to provide confirm the sequence of a purified protein or to evaluate the binding of a ligand to a purified protein. In our experience, intact mass measurements are a invaluable in the determination of an unknown antibody sequence.

Steps Involved

1) Sample preparation:... Show more »

Overview

An intact mass measurement can be used to provide confirm the sequence of a purified protein or to evaluate the binding of a ligand to a purified protein. In our experience, intact mass measurements are a invaluable in the determination of an unknown antibody sequence.

Steps Involved

1) Sample preparation: Dilution of submitted sample or custom buffer exchange.
2) LC-MS/MS: 0.5hr LC-MS/MS
3) Data analysis: Thermo BioPharma Finder
4) Report generation: PDF

The turnaround time is 2-3 weeks.

Deliverable

1) Written Report containing details of your samples, our methods and a summary of the data. If the sequence of the target is provided we can compare the calculated average mass with the measured mass. This also presents the opportunity to consider post-translational modifications such as phosphorylation or chemical derivatives i.e. covalently bound small molecules, drugs, dyes etc.
An example report is available upon request.

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Post-Translational Modification Analysis
Starting at $310.00 per sample

Overview

This service enables the identification, localization and quantitation of post-translational modifications on a protein using a panel of three enzymes to maximize sequence coverage of the target protein.

Steps Involved

1) Sample preparation: Trypsin digestion of the submitted gel band.
2) LC-MS/MS: 0.5hr... Show more »

Overview

This service enables the identification, localization and quantitation of post-translational modifications on a protein using a panel of three enzymes to maximize sequence coverage of the target protein.

Steps Involved

1) Sample preparation: Trypsin digestion of the submitted gel band.
2) LC-MS/MS: 0.5hr LC-MS/MS
3) Data analysis: Protein and PTM identification is performed with Mascot. Protein visualization and validation is performed with Scaffold. PTM Validation is performed with Scaffold PTM.
Percentage occupancy can be approximated using Byologic (ProteinMetrics)
4) Report generation: PDF/Excel/Scaffold + Scaffold PTM

The turnaround time is 2-3 weeks.

Deliverable

1) Written Report containing details of your samples, our methods and a summary of the data.
2) An Excel workbook containing the detailed qualitative and quantitative protein identification data.
3) Scaffold and Scaffold PTM files to enable your own deeper data mining.

An example report is available upon request.

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Protein Expression Profiling
Starting at $650.00 per sample

Overview

The MSB-05, 06, 07, and 08 services are designed to enable protein profiling of samples of vary complexity. The services differ in the total amount of instrument time dedicated to an analysis. The amount of instrument time for each service is 1, 5, 10 and 20hrs for MSB-05, 06, 07 and 08, respectively. Broadly speaking... Show more »

Overview

The MSB-05, 06, 07, and 08 services are designed to enable protein profiling of samples of vary complexity. The services differ in the total amount of instrument time dedicated to an analysis. The amount of instrument time for each service is 1, 5, 10 and 20hrs for MSB-05, 06, 07 and 08, respectively. Broadly speaking the greater the amount of instrument time, the greater the number of proteins identified and higher the sequence coverage of those proteins.

Steps Involved

1) Sample preparation including SDS-PAGE with multiple band excision and in-gel digestion.
2) LC-MS/MS: Increments of 0.5hr LC-MS/MS.
3) Data analysis: Protein identification is performed with Mascot. Protein visualization and validation is performed with Scaffold.
4) Report generation: PDF/Excel/Scaffold

The turnaround time is 2-3 weeks.

Deliverable

1) Written Report containing details of your samples, our methods and a summary of the data.
2) An Excel workbook containing the detailed qualitative and quantitative protein identification data.
3) A Scaffold file to enable your own deeper data mining.

An example report is available upon request.

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Phosphoproteomics
Starting at $2,400.00 per sample

Overview

This service enables a global view of protein and site specific phosphorylation events using a workflow optimized for a single shot 2hr-LC-MS/MS analysis. With lower costs, higher throughput and high sensitivity this workflow is rapidly emerging as an industry favorite for the study of systems wide modulation of... Show more »

Overview

This service enables a global view of protein and site specific phosphorylation events using a workflow optimized for a single shot 2hr-LC-MS/MS analysis. With lower costs, higher throughput and high sensitivity this workflow is rapidly emerging as an industry favorite for the study of systems wide modulation of phosphorylation.

Steps Involved

1) Sample preparation: Protein extraction, solution digestion with trypsin, enrichment of phosphopeptides with titanium dioxide.
2) LC-MS/MS: 2hr LC-MS/MS using a ThermoFisher Fusion Lumos.
3) Data analysis. Phosphorylation site identification quantitation with MaxQuant. Differential analysis with Perseus.
4) Report generation: PDF/Excel

The turnaround time is 2-3 weeks.

Deliverable

1) Written Report containing details of your samples, our methods and a summary of the data.
2) An Excel workbook containing the detailed qualitative and quantitative phosphorylation analysis data.

An example report is available upon request.

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SILAC
Stable Isotope Labeling by Amino Acids in Cell Culture
Starting at $4,100.00 per sample

Overview

This service is designed for the quantitative and qualitative analysis of cell based systems. The results from this experiment provide a catalogue of the proteins present in all samples and a statistical analysis reflecting the changes in the protein levels observed across the samples, along with associated pathway... Show more »

Overview

This service is designed for the quantitative and qualitative analysis of cell based systems. The results from this experiment provide a catalogue of the proteins present in all samples and a statistical analysis reflecting the changes in the protein levels observed across the samples, along with associated pathway information. MS Bioworks also offers a complete SILAC service including cell culture, treatment of cells, and proteomics analysis, please call or email for details.

Steps Involved

1) Label incorporation analysis.
2) Sample preparation. Protein extraction, mixing of unlabeled (Light) and labeled (Heavy) trypsin digestion, peptide fractionation and pooling using a custom pooling matrix.
3) LC-MS/MS. 8x3hr LC-MS/MS using a ThermoFisher Fusion Lumos mass spectrometer.
4) Data analysis: Protein Identification and quantitation with MaxQuant. Differential analysis with Perseus.
5) Report generation: PDF/Excel

The turnaround time is 2-3 weeks.

Deliverable

1) Written Report containing details of your samples, our methods and a summary of the data.
2) An Excel workbook containing the detailed qualitative and quantitative protein identification data.

An example report is available upon request.

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Tandem Mass Tags
Tandem Mass Tags
Starting at $8,700.00 per sample

Overview

This service is designed for the quantitative and qualitative analysis of cell based systems. The results from this experiment provide a catalogue of the proteins present in all samples and a statistical analysis reflecting the changes in the protein levels observed across the samples, along with associated pathway... Show more »

Overview

This service is designed for the quantitative and qualitative analysis of cell based systems. The results from this experiment provide a catalogue of the proteins present in all samples and a statistical analysis reflecting the changes in the protein levels observed across the samples, along with associated pathway information. MS Bioworks also offers a more comprehensive service including cell culture, treatment of cells, and proteomics analysis, please call or email for details.

Steps Involved

1) Label incorporation analysis.
2) Sample preparation. Protein extraction, trypsin digestion, peptide labeling, mixing of labeled peptides , labeled peptide fractionation and pooling using a custom matrix.
3) LC-MS/MS. 8x3hr LC-MS/MS using a ThermoFisher Fusion Lumos mass spectrometer.
4) Data analysis: Protein Identification and quantitation with MaxQuant. Differential analysis with Perseus.
5) Report generation: PDF/Excel

The turnaround time is 2-3 weeks.

Deliverable

1) Written Report containing details of your samples, our methods and a summary of the data.
2) An Excel workbook containing the detailed qualitative and quantitative protein identification data.

An example report is available upon request.

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Histone Modification Analysis
Starting at $8,850.00 per sample

Overview

This service enables a quantitative comparison of two samples for global histone marks. Histones are isolated from cells or tissue followed by quantitative mass spectrometry analysis in a single pairwise experiment using the “One-Pot” approach* with light and heavy propionylation.

Overview

This service enables a quantitative comparison of two samples for global histone marks. Histones are isolated from cells or tissue followed by quantitative mass spectrometry analysis in a single pairwise experiment using the “One-Pot” approach* with light and heavy propionylation.

  • One-Pot Quantitative Mass Spectrometry Characterization of Histones”, Garcia et al., J. Proteome Research, 8, 5367-5374(2009).

Steps Involved

1) Isolation of histones from cells or tissue using the Active Motif Histone Purification Kit. Submission of 10million cells or 25mg of tissue (or greater) is required.
2) Block lysine side chains using d0 propionic anhydride.
3) Solution digestion with trypsin.
4) Label peptide N-terminii with either d0 (sample 1) or d10 (sample 2) propionic anhydride.
Mix samples 1:1.
5) 90min LC/MS/MS in analytical duplicate on Orbitrap mass spectrometer.
6) Peak integration for over 70 peptide/PTM combinations across H2A, H2B, H3 and H4.
7) Differential analysis.

Deliverable

1) Written report containing details of your samples, our methods and a summary of the data.
2) An Excel workbook containing the detailed quantitative data.

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Protein Sequence Validation
Starting at $800.00 per sample

Overview
The MSB-03 service is intended to provide sequence confirmation data and requires three gel bands containing the target. Each band should contain at least 100ng of the target protein.

Steps Involved
Sample preparation: In-gel digestion with trypsin, chymotrypsin and elastase.
LC-MS/MS: 3 x 0.5hr LC-MS/MS
Data... Show more »

Overview
The MSB-03 service is intended to provide sequence confirmation data and requires three gel bands containing the target. Each band should contain at least 100ng of the target protein.

Steps Involved
Sample preparation: In-gel digestion with trypsin, chymotrypsin and elastase.
LC-MS/MS: 3 x 0.5hr LC-MS/MS
Data analysis: Protein identification is performed with Mascot. Protein visualization and validation is performed with Scaffold.
Report generation: PDF/Excel/Scaffold
The turnaround time is 2-3 weeks.

Deliverables
Written Report containing details of your samples, our methods and a summary of the data. The sequence coverage map obtained for the target is provided in the report.
An Excel workbook containing the detailed qualitative and quantitative protein identification data.
A Scaffold file to enable your own deeper data mining.
An example report is available upon request.

« Show less
2018-11-15 07:12:27 -0800

Net Promoter Score of 10 received for Post-Translational Modification Analysis.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-10-26 07:47:13 -0700

Net Promoter Score of 8 received for LC-MS.

Additional Ratings: satisfaction with deliverable: 7, satisfaction with timeliness: 7.
2018-04-20 08:34:56 -0700

Net Promoter Score of 6 received for Proteomics.

Additional Ratings: satisfaction with deliverable: 6, satisfaction with timeliness: 10.
2017-12-19 08:42:43 -0800

Net Promoter Score of 10 received for Protein Identification.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.

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