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Mouse Genome Engineering Core

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Dallas, Texas, US

About Mouse Genome Engineering Core

The CRI Mouse Genome Engineering Core Facility is committed to helping researchers produce mouse models to support fundamental and translational research, providing transgenic, gene targeting and other services. Established in 2014, the facility serves scientists at CRI, UT Southwestern and other... Show more ยป

The CRI Mouse Genome Engineering Core Facility is committed to helping researchers produce mouse models to support fundamental and translational research, providing transgenic, gene targeting and other services. Established in 2014, the facility serves scientists at CRI, UT Southwestern and other institutes.

The Mouse Genome Engineering Core Facility performs DNA microinjection services, CRISPR/Cas9 microinjection services, ES cell manipulation and other microinjection services to generate transgenic mice, knock-in/knock-out mice and chimeric mice harboring select genetic mutations. Other services include mouse sperm cryopreservation and recovery, in vitro fertilization (IVF), rederivation of pathogen free mouse lines and derivation of mouse embryonic stem cell lines.

Our staff provides general consultations on experimental designs and vectors for gene modification-related projects, DNA preparation, recombinant ES clones, mouse genotyping, colony breeding and husbandry. We can also customize services as requested to support the development of animal models for modeling human diseases.

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Our Services (18)


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Transgenic Animal Model Development

Starting at $4,500.00 per study

We predominantly use the Easi-Crispr method to generate point mutations, targeted knockins, and floxed alleles. Please refer to https://www.nature.com/articles/nprot.2017.153 for details about Easi-Crispr.

Our average turnaround time to get founders is 50 days
Price includes ssDNA, cRNA and Cas9 protein
For that price, you will get 3 founders or injection or 500 embryos (what ever come first)

We predominantly use the Easi-Crispr method to generate point mutations, targeted knockins, and floxed alleles. Please refer to https://www.nature.com/articles/nprot.2017.153 for details about Easi-Crispr.

Our average turnaround time to get founders is 50 days
Price includes ssDNA, cRNA and Cas9 protein
For that price, you will get 3 founders or injection or 500 embryos (what ever come first)

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Genetically Engineered Mice

Starting at $4,500.00 per study

Production of transgenic mice using pronuclear injection of DNA, including CRISPR's.

Production of transgenic mice using pronuclear injection of DNA, including CRISPR's.

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Knock-In Mouse Development

Starting at $4,500.00 per study
Request a quote for more information about this service.

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Knockout Mouse Development

Starting at $4,500.00 per study
Request a quote for more information about this service.

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Embryonic Stem Cell Gene Targeting

Starting at $7,500.00 per study
Request a quote for more information about this service.

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Blastocyst Injection

Starting at $8,000.00 per study

Targeted ES cells (two clones from the same targeting construct) will be microinjected into C56Bl/6 (black 6) blastocysts, after which they will be implanted into pseudopregnant females. Any resulting male pups which are >40% chimeric will be bred to test for germline transmission. It is recommended that targeted ES clones by karyotyped prior to microinjection.

Targeted ES cells (two clones from the same targeting construct) will be microinjected into C56Bl/6 (black 6) blastocysts, after which they will be implanted into pseudopregnant females. Any resulting male pups which are >40% chimeric will be bred to test for germline transmission. It is recommended that targeted ES clones by karyotyped prior to microinjection.

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Animal Embryo and Sperm Cryopreservation

Starting at $1,500.00 per study

For mouse

For mouse

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Rodent Rederivation

Starting at $2,000.00 per study

In vitro fertilization to rederive mouse lines from frozen or fresh sperm.

In vitro fertilization to rederive mouse lines from frozen or fresh sperm.

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Rodent in vitro Fertilization

Starting at $2,000.00 per study

In vitro fertilization to rederive mouse lines from frozen or fresh sperm.

In vitro fertilization to rederive mouse lines from frozen or fresh sperm.

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Intracytoplasmic Sperm Injection

Starting at $2,000.00 per study

Intracytoplasmic sperm injection to rederive mouse lines from un-efficient frozen or fresh sperm.

Intracytoplasmic sperm injection to rederive mouse lines from un-efficient frozen or fresh sperm.

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Cryopreservation

Starting at $1,500.00 per strain
Request a quote for more information about this service.

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Transgenic Animal Colony Management

Starting at $1,500.00 per study

Our colony management service offerings include:

  • Breeding
  • Sperm cryopreservation
  • Cryorecovery
  • Rederivation
  • IVF
  • Intracytoplasmic sperm injection (ICSI)

Our colony management service offerings include:

  • Breeding
  • Sperm cryopreservation
  • Cryorecovery
  • Rederivation
  • IVF
  • Intracytoplasmic sperm injection (ICSI)
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Cryostorage and Cryoprocessing

Starting at $350.00 per strain

Cryostorage Services per year

Cryostorage Services per year

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CRISPR/Cas9 Genome Editing

Price on request
Request a quote for more information about this service.

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Transgenic Mouse Services

Price on request
Request a quote for more information about this service.

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Genetic Engineering

Price on request

Genetic Engineering Services

Genetic Engineering Services

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Biology

Price on request
Request a quote for more information about this service.

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Biospecimen Management and Storage

Price on request

Biospecimen Management and Storage Services

Biospecimen Management and Storage Services

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Nicolas Loof

CRI Research Shared Facilities Manager

Mouse Genome Engineering Core has not received any reviews.

Mouse Genome Engineering Core has not received any endorsements.