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Mouse Cardiovascular Phenotyping Core

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St. Louis, Missouri, US

About Mouse Cardiovascular Phenotyping Core

The Washington University Mouse Cardiovascular Phenotyping Core (MCPC) is a leading research core that was established in 1998 and currently provides approximately 3,000 surgical, imaging and functional cardiovascular physiology services annually to its academic and industry partners throughout the world. With... Show more »

The Washington University Mouse Cardiovascular Phenotyping Core (MCPC) is a leading research core that was established in 1998 and currently provides approximately 3,000 surgical, imaging and functional cardiovascular physiology services annually to its academic and industry partners throughout the world. With an experienced staff of 4 dedicated investigators, each with over 10 years of animal physiology experience, the MCPC provides a focused, systematic approach to the study of the basal physiology and pathophysiology of the mouse cardiovascular system.

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Our Services (15)


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Animal Surgery

Price on request

Transverse Aortic Constriction (TAC):
Operative placement of constriction on the transverse aorta. Includes recording mouse weight, echocardiography at study end, euthanasia at study end, and if desired, organ harvest and determination of left ventricular weight. Additional echocardiographic studies can be requested. Study end... Show more »

Transverse Aortic Constriction (TAC):
Operative placement of constriction on the transverse aorta. Includes recording mouse weight, echocardiography at study end, euthanasia at study end, and if desired, organ harvest and determination of left ventricular weight. Additional echocardiographic studies can be requested. Study end will be 1-week post TAC unless requested otherwise.
Data provided at study end: Spreadsheet with end echo parameters, mouse weight before surgery, surgical intervention (sham or band), and heart weight.
Neonatal Ascending Aortic Banding (NAB):
Operative placement of a “loose” constriction on the ascending aorta of 18-22 day old mice only. Includes recording mouse weight, echocardiography at study end (8-12 weeks), euthanasia at end of study, and if desired, organ harvest and determination of left ventricular weight. Additional echocardiographic studies can be requested. Study end will be two months post NAB unless requested otherwise.
Data provided at study end: Spreadsheet with end echo parameters, mouse weight before surgery, surgical intervention (sham or band), and heart weight.

Adult Ascending Aortic Banding (AAB):
Operative placement of constriction on ascending aorta of adult mice (preferably 18-28 gram weight). Includes recording mouse weight, 1 echo study at experiment end time, and if desired, euthanasia at study end with organ harvest and determination of left ventricular weight. Additional echocardiographic studies can be requested. Study end will be one month post AAB unless requested otherwise.
Data provided at study end: Spreadsheet with end echo parameters, mouse weight before surgery, surgical intervention (sham or band), and heart weight.

Myocardial Infarction (MI):
Operative placement of a permanent occlusion around the left anterior descending artery of the mouse heart. Includes recording mouse weight, echocardiography at study end, and if desired, euthanasia at study end with organ harvest and left ventricular weight measurements. Additional echocardiographic studies can be requested. Study end will be one month post MI unless requested otherwise.
Data provided at study end: Spreadsheet with end echo parameters including regional wall differences, mouse weight before surgery, and surgical intervention (sham or MI).

Myocardial Ischemia/Reperfusion (with image analysis):
Operative placement of an occluder around the left anterior descending artery that is removed after 30 minutes of occlusion. The heart is allowed to reperfuse for 24 hours and is then stained to calculate a normal region, area at risk, and infarcted region. Image analysis is then performed and an end data report is provided documenting these areas as a % of the heart. No echo is included.
Data provided at study end: Images of the staining distribution in each heart slice and spreadsheet documenting %Area at risk and Infarct% of the area at risk.
Myocardial Ischemia/Reperfusion (without image analysis):
Operative placement of an occluder around the left anterior descending artery that is removed after 30 minutes of occlusion. The heart is allowed to reperfuse for 24 hours and is then stained with vital dyes to delineate a normal region, area at risk, and infarcted region. No image analysis or echo is included.
Data provided at study end: No data, just a heart in formalin that has been stained with vital dyes to delineate normal, risk, and infarcted tissue.

Tissue Fixation (in vivo heart perfusions) for Histology:
Entails carotid catheter placement, fixative injection into the heart, euthanasia and organ harvest.
Data provided at study end: A heart in formalin.

Customized Surgeries:
Specialized surgery not listed above but agreed upon by surgeon and investigator.

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Animal Electrocardiography

Price on request

Implantation of ECG Transmitters and ECG Recording:
This procedure includes implantation of transmitters, post-operative single ECG recording for 24 hours and transmitter removal. Additional recording time is available. Heart rates/representative rhythm strips are provided at study end. Services do not include arrhythmia... Show more »

Implantation of ECG Transmitters and ECG Recording:
This procedure includes implantation of transmitters, post-operative single ECG recording for 24 hours and transmitter removal. Additional recording time is available. Heart rates/representative rhythm strips are provided at study end. Services do not include arrhythmia monitoring or identification of ectopy.
Data provided at study end: Summary report including surgery, survival, implant information and recording dates; spreadsheets of scheduled sampling times, heart rates and averages; printouts of representative ECG traces; and ascii files of complete data sets upon request.

Implantation of Blood Pressure Transmitters and Blood Pressure Recording:
This procedure includes implantation of transmitters and carotid artery catheterization with a polyurethane ticking. Post-operative carotid pressure recordings for a single 24 hr recording session and eventual transmitter removal are included. Blood pressure fluctuations are recorded and data is collected and categorized. Blood pressure wave forms are provided at study end.
Cost includes refurbishment of blood pressure catheter. Cost reflects directly the amount we are charged for refurbs and may, therefore, be increased without advanced notice if the amount charged by Data Sciences increases.
Data provided at study end: Summary report including surgery, survival, implant information and recording dates; spreadsheets of scheduled sampling times, mean pressures, systolic and diastolic pressures, pulse pressures, heart rates and averages; printouts of representative blood pressure traces; and ascii files of complete data sets upon request.

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Animal Exercise Testing

Price on request

Our facility features dedicated exercise equipment for mice:
4-lane automated exercise treadmill apparatus
swimming pool
self-governing exercise wheel

Endpoints to these studies can include:
willingness to (and duration of) exercise
survival
documentation of rhythm disturbances
ventricular function assessment

Mice are... Show more »

Our facility features dedicated exercise equipment for mice:
4-lane automated exercise treadmill apparatus
swimming pool
self-governing exercise wheel

Endpoints to these studies can include:
willingness to (and duration of) exercise
survival
documentation of rhythm disturbances
ventricular function assessment

Mice are monitored continuously during all exercise regimens by trained personnel.

Tech Assisted Treadmill and Swimming:

Treadmill:
This service employs the use of a motorized, speed controlled modular treadmill system designed specifically for mice. The treadmill is equipped with an electric shock stimulus for motivation and an adjustable inclination angle. The treadmill can be used for both long and short-term exercise studies. A technician is provided to conduct the study and gather date OR you may request full training on the use of the treadmill and for future studies conduct exercise studies without a provided technician.

Swimming:
This service employs the use of a specifically dimensioned container that is filled with 35 degree water to a depth of 25 cm. Mice swim in groups of 5-8 per swim. A light current is kept running to stimulate the desire to swim. The pool can be used for both stress and long term exercise studies. A technician is provided to conduct the study and gather data and watch the mice closely to prevent drowning OR you may request full training on the use of the swimming pool and for future studies conduct swims without a provided technician.
Data provided at study end: Spreadsheet documenting total time of exercise, failure periods, speed, and incline.

Other Services Available:
Mandatory One-Time Tech-Assisted Training (if planning use of equipment without a tech in future studies)
Facility Charge for Equipment Use without a Tech
Organ Harvest after Exercise

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Animal Hemodynamics

Price on request

Hemodynamics I:
Includes surgical cutdown, Millar catheter placement, and recording of aortic pressures only.
Data provided at study end: Spreadsheet with aortic systolic and diastolic pressure measurements and heart rate.

Hemodynamics II:
Includes surgical cutdown, Millar catheter placement in left ventricle, recording... Show more »

Hemodynamics I:
Includes surgical cutdown, Millar catheter placement, and recording of aortic pressures only.
Data provided at study end: Spreadsheet with aortic systolic and diastolic pressure measurements and heart rate.

Hemodynamics II:
Includes surgical cutdown, Millar catheter placement in left ventricle, recording of LV pressures and derivation of LV peak pressures, LV end-diastolic pressure, ± dp/dt, and tau.
Data provided at study end: Spreadsheet with measurements of various LV pressure-derived hemodynamic variables, including Heart Rate, LV peak pressure, LV end diastolic pressure, Peak +dP/dt, Peak –dP/dt, and tau. A customized spreadsheet is also available.

Hemodynamics III:
Hemodynamics III includes surgical cutdown, Millar catheter placement into left ventricle and recording of LV pressure under multiple physiologic conditions such as pre-post pharmacologic interventions, plus derivation of standard indices of LV function for each condition.
Data provided at study end: Spreadsheet with various LV pressure-derived variables assessed at different time points and/or under different physiological conditions. The spreadsheet will include Heart Rate, LV peak pressure, LV end diastolic pressure, Peak +dP/dt, Peak –dP/dt, and tau. A customized spreadsheet is also available.

Hemodynamics IV:
Includes surgical cutdown, Millar pressure-volume catheter placement into the left ventricle and recording of LV pressures and volumes under multiple physiologic conditions. Analysis includes derivation of indexes of LV function derived from simultaneous LV pressure-volume recordings (load-independent measurements).
Data provided at study end: Spreadsheet with various LV PV-derived variables assessed at different time points and/or under different physiological conditions. A customized spreadsheet is also available.

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Animal Organ Harvesting

Price on request
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Animal Echocardiography

Price on request

Non-invasive transthoracic cardiac ultrasound exam performed under light general anesthesia (unless status of animal precludes anesthesia). A VisualSonics cardiac echocardiography machine with a 15MHz linear transducer is used to characterize the structure and function of the heart and great vessels.

Single Screening... Show more »

Non-invasive transthoracic cardiac ultrasound exam performed under light general anesthesia (unless status of animal precludes anesthesia). A VisualSonics cardiac echocardiography machine with a 15MHz linear transducer is used to characterize the structure and function of the heart and great vessels.

Single Screening Echocardiogram:
Non-invasive single transthoracic cardiac ultrasound exam performed under light general anesthesia that includes two-dimensional and M-mode images of the left ventricle.
Data provided at study end: Spreadsheet with various echo parameters and representative images.

Post-Surgical (after TAC, AAB, or NAB)/Tissue Doppler/Diastolic Function Echocardiograms:
Full echocardiograms to document the outcome of a specific surgery other than myocardial infarction (such as TAC or NAB). This echo may include two-dimensional and M-mode images and analysis of the left ventricle, or Doppler recordings of trans-valvular flow indices (gradients), or analysis of diastolic function.
Data provided at study end: Spreadsheet with various echo parameters and representative images. Data on spreadsheet will include gradient measurements at the site of constriction.

Post-Infarction Echocardiograms:
Echo which requires more than M-mode and two-dimensional images because of the presence of regional wall motion abnormalities (e.g. myocardial infarction studies). These echoes are performed on the Visual Sonics echo machine.
Data provided at study end: Spreadsheet with various echo parameters and representative images. Data on spreadsheet will include segmental wall motion score index, LV end diastolic and systolic volumes, ejection fraction and heart rate.

Vascular Echocardiogram using Visual Sonics Echo Machine:
Echo to delineate structural and functional abnormalities of the aorta or other vessels. Includes an analysis of these parameters and data reporting.

Customized Echocardiography using the Visual Sonics Echocardiography Machine:
Includes studies that the echocardiographer and PI have discussed in detail and that are not offered on a routine basis. Analysis and data reporting will be included.

Emergency Echocardiograms:
Previously unscheduled echoes that are necessary to do immediately because mouse is in newly discovered end-stage heart failure and may expire, or other emergent condition.
Data provided at study end: Spreadsheet with various echo parameters and representative images.

Alternate Species Echocardiograms:
Echocardiograms of any species other than mice.

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Animal Motor Function Tests

Price on request
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Animal Physiology Analyses

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Animal in vivo Imaging

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Biology

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Animal Cognition & Behavior Tests

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Animal Model in vivo Analyses

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Animal Models and Studies

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Cells and Tissues

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Discovery Pathology

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