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MoonElisa Biotechnology

Baton Rouge, Louisiana, US

MoonElisa Biotechnology, was founded in 2014 (Baton Rouge, Louisiana) to develop and manufacture ELISA technology kits for industrial and R&D laboratories. Our mission is to provide high quality ELISA reagents to researchers worldwide that empowers the process of scientific finding in the areas of allergy, weight regulation, apoptosis, diabetes, wound healing, signal transduction, metabolism, cancer, immunology, and neurosciences. MoonElisa believes that maintaining earned trust is a combination of innovation and continuous commitment to quality.

MoonElisa's ELISA Advantages:

  • Fast results with high precision and accuracy
  • Affordable prices
  • Easy to use
  • Liquid substrate and buffers provided with each kit
  • Pre-coated 96-well plates
  • High sensitivity
  • Quantitative results
  • Non-radioactive
  • Reproducible
  • Upon client's request, recommended extraction procedures will be provided.

We... Show more »

MoonElisa Biotechnology, was founded in 2014 (Baton Rouge, Louisiana) to develop and manufacture ELISA technology kits for industrial and R&D laboratories. Our mission is to provide high quality ELISA reagents to researchers worldwide that empowers the process of scientific finding in the areas of allergy, weight regulation, apoptosis, diabetes, wound healing, signal transduction, metabolism, cancer, immunology, and neurosciences. MoonElisa believes that maintaining earned trust is a combination of innovation and continuous commitment to quality.

MoonElisa's ELISA Advantages:

  • Fast results with high precision and accuracy
  • Affordable prices
  • Easy to use
  • Liquid substrate and buffers provided with each kit
  • Pre-coated 96-well plates
  • High sensitivity
  • Quantitative results
  • Non-radioactive
  • Reproducible
  • Upon client's request, recommended extraction procedures will be provided.

We constantly strive to provide only the best through our affordable pricing, reliable service, high quality, and courteous care to our customers. By providing a consultative approach, superior technical and customer support, and smooth purchasing options, MoonElisa is continually working side by side with our customers to help solving their scientific and clinical challenges.

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MoonElisa Biotechnology has not listed any services.

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ELISA
Enzyme-linked immunosorbent assay
Starting at $1.75 per sample

ELISA Testing Services
"We know ELISA is a time consuming assay, we want you to concentrate on something else while we are doing this assay for you. Please let us do it for you".
MoonElisa offers in-house testing services for protein identification and quantification. We offer a two-working-day turnaround time on... Show more »

ELISA Testing Services
"We know ELISA is a time consuming assay, we want you to concentrate on something else while we are doing this assay for you. Please let us do it for you".
MoonElisa offers in-house testing services for protein identification and quantification. We offer a two-working-day turnaround time on results from the time we receive a sample on most tests. Same day rush is available for samples received before (10:30am Central). We mail the original report to the listed contact person. Our testing services are highly confidential. Information is only reported to the requesting company and then only to their indicated contact persons.

Our expertise laboratory offers full testing for all ELISA kits that were listed in our website. Customer can also send us its own antibodies and/or standards to be used in our ELISA testing services, in this instance; a discounted quotation will be applied. The testing will be conducted at our facility.
*Note: Please note that samples run at several dilutions or re-running samples at different dilutions will be considered as additional samples (40 samples/plate).

No. of Samples
> 61 samples: $149.0 /ELISA plate + $1.75/sample
41-60 samples: $149.0 /ELISA plate + $3.0/sample
31-40 samples $149.0 /ELISA plate + $5.0/sample
17-30 samples : $149.0 /ELISA plate + $7.0/sample.
1-16 samples: $115.0 /ELISA plate + $9.0/sample.

Please include a printed copy of the Sample Testing Form with your shipment. Samples must be free of infections or any hazardous materials, such as HIV or other communicable viruses, etc. Please call us at 225-330-3153 or inform us by e-mail when you send your samples ([email protected]). Please include a tracking number for the shipment. MoonElisa will store your samples appropriately for four weeks, Fees will be applied upon customer request after four weeks for any extended storage time. Please send your samples frozen or refrigerated shipping to:

MoonElisa Biotechnology LLC
LSU-LETC -Louisiana Emerging Technology Center
Building 340 East Parker Blvd.
Laboratory 240 (second floor)
Baton Rouge, LA 70803

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Immunoassay Consulting
Starting at $75.00 per hour

Consultations: Using sound statistical methodologies during assay development should lead to reduced development time and reduced overall cost.

MoonElisa offers immunoassay consulting and troubleshooting services in the areas of assay development, assay validation, basic R&D and other protein characterization and... Show more »

Consultations: Using sound statistical methodologies during assay development should lead to reduced development time and reduced overall cost.

MoonElisa offers immunoassay consulting and troubleshooting services in the areas of assay development, assay validation, basic R&D and other protein characterization and processing techniques.Our consulting services are provided in the following areas:

  • Immunochemistry: direct, indirect, sandwich, competitive, and inhibition ELISAs, Cell based Bio-assay, ELISA cell based assay, western blot, dot blot, and neutralizing assays.
  • Protein Processing: protein precipitation, concentration, deglycosylation, de-silalylation, denaturation, reduction, alkylation, digestion, MW cut off filteration, aggregation, and formulation.
  • Protein Characterization: Capillary Electrophoresis, peptide mapping, zone electrophoresis, IEF, RP-HPLC, IEX chromatography, SEC, affinity chromatography, hydrophobic interaction chromatography, high throughput protein-binding and formulation experiments.
  • Statistics: ANOVA, ANCOVA, correlation, general linear models, polynomial regression, 4-Parameter Logistics models, SAS code and programming, DOE, Fixed Model analysis, Random Model analysis, Mixed Model Analysis, Varaince components determination, Z-test, t-test, Q-value testing, p-value interpretation and determination, Least Square analysis, Maximum Liklihood analysis, Restriction Maximum Liklihood analysis, outliers determination, normality, linear model regression, multiple regression models, multivariate analysis, and basic statistics parameters (average, varaince, coefficient of correlation, standard deviation, standard errors, coefficient of determination, degree of freedom, median, propability, combinations, and numerical analysis)

MoonElisa's specialty is working with startups and local research laboratories in assessing their projects to achieve the highest rate of success possible. Our systematic approach of consultation begins with the client's objective and emphasizes product and process understanding and process control, based on sound science, extensive data mining, and quality risk management.

  1. Ask a question
  2. Do background research
  3. Construct a hypothesis by establishing your outputs
  4. Test your hypothesis by DOE (statistics)
  5. Analyze your data and draw conclusion (statistics)
  6. Communicate your results (statistics)
  7. Conclude and document

We know that the statistical considerations for assay development, qualification, and assay validation are important to keep in mind during assay and product development. Whether used in basic research or clinical diagnosis, assays require careful development processes to ensure that the assays will consistently perform to specifications and that they will be effective for their intended use after validation. In addition, using sound statistical methodologies during assay development should lead to reduced development time and reduced overall cost.

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Custom Cell-Based Assays
Starting at $5,000.00 per unit

*ELISA Optimization: MoonElisa Biotech provides Contract ELISA Development services for R&D laboratories and industrial companies. We provide custom services that will meet your specific needs. If you need to detect or measure a specific target molecule, we can fully develop a quick and accurate immunoassay test or optimize an... Show more »

ELISA Optimization: MoonElisa Biotech provides Contract ELISA Development services for R&D laboratories and industrial companies. We provide custom services that will meet your specific needs. If you need to detect or measure a specific target molecule, we can fully develop a quick and accurate immunoassay test or optimize an existing assay according to your specifications.

We will assemble your reagents into a highly optimized sandwich ELISA assay.

Our optimization includes the following:

  • Optimizing capture antibody concentration
  • Optimizing the solid phase binding pH and kinetics
  • Optimizing the blocking buffer
  • Optimizing the standard diluents
  • Optimizing sample and conjugate matrix composition
  • Optimizing sample concentration
  • Optimizing the antigen incubation times
  • Optimizing the detection antibody concentration
  • Optimizing the enzyme conjugate concentration
  • Optimizing conjugate incubation times
  • Optimizing signal detection

To proceed in this inquiry, please follow the following easy steps:

  • Based on your request and specific needs, assay optimization begins with a consultation with our scientific team.
  • Method feasibility is determined based on the availability of assay components and reagents with the desired assay specific parameters. MoonElisa generally charges $75 an hour for consultation, reviewing publications, design of experiment, statistical analysis, and sourcing reagents.
  • MoonElisa can provide a quote of estimated time and expenses and will discuss a specific payment schedule with each customer. MoonElisa will email you a detailed quote for your specific assay optimization project including our feasibility study. MoonElisa generally charges $75 an hour for quote preparation and conducting a feasibility study for your product.
  • After signing the approval agreement with us, you initiate a request for our ELISA Method Development.
  • A comprehensive protocol for this custom ELISA optimization will be provided at the end of our service.
  • Once the assay has being optimized, we will ship the kit components for your on-site testing.

Example of ELISA Optimization Project:

  • Objective: To improve the sensitivity of an ELISA that is specific for an anlyte approximately 50 fold.
  • Performance Specification: Analytical sensitivity < 20 pg/mL
  • Proposed Timeline: 8 Weeks
  • Description:
    • In Phase 1, The customer's ELISA will be established and the key assay parameters (reagent concentrations, volumes, and assay incubation times) will be optimized. MoonELISA will evaluate the use of sandwich ELISA immunoassay formats employing different detection systems to achieve the required sensitivity.
    • In Phase 2, additional antibodies were evaluated in sandwich formats for the required sensitivity.
  • Results: Antibodies will be identified that are suitable for the detection of the analyte in sandwich ELISA formats. We identified and optimized an antibody pair that when used in a sandwich immunoassay format achieved an analytical sensitivity of 2 to 10 pg/mL.
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Experimental Design
Starting at $5,000.00 per unit

Assay Validation Services: Upon the client's request, MoonElisa provides documented evidence of validation studies and comprehensive validation reports.

MoonElisa knows that good science requires well-planned, well-executed, and well-documented experiments that are conducted under good laboratory practices, followed by a... Show more »

Assay Validation Services: Upon the client's request, MoonElisa provides documented evidence of validation studies and comprehensive validation reports.

MoonElisa knows that good science requires well-planned, well-executed, and well-documented experiments that are conducted under good laboratory practices, followed by a meaningful interpretation of data. Experiments should be based on generally accepted scientific principles, and appropriate controls should be included to demonstrate that the experimental setup is working as expected. To get a qoute for our assay validation, please follow the following steps:

  • Based on your request and specific needs, assay validation will begin with a consultation with our scientific team.
  • MoonElisa can provide a quote of estimated time and expenses and will discuss a specific payment schedule with each customer. MoonElisa will email you a detailed quote for your specific assay validation project including our feasibility study for your request. MoonElisa generally charges $75 an hour for quote preparation and conducting a feasibility study.
  • All experiments conducted for the purpose of test method validation will be performed using established Standard Operation Procedures for the quantitative evaluation of any given analyte. The cost for the assay validation typically ranges from $3,000 to $50,000 and usually takes 6-24 weeks. This price depends on what stage of validation is being requested (see below) and on the expense of the assay kit components.
  • After signing the approval agreement with us, MoonElisa will proceed with your assay validation request.
  • At the end, MoonElisa will provide documented evidence of validation studies and comprehensive validation reports.

MoonElisa Methodology for bioanalytical assay validation includes all of the procedures that demonstrate that a particular method used for quantitative measurement of analytes in a given biological matrix, such as media, cell extract, blood, plasma, serum, or any biological fluids, is reliable and reproducible for the intended use.

There are several stages taken in a validation process in order to qualify an assay as suitable for its use. The validation stages are summarized as follow:

  • Stage 1. Selection and feasibility
  • Stage 2. Purpose and scope
    • Method selection
    • Reference standard selection
    • Method outline
    • Calculation of an analyte
  • Stage 3. Development and standardization
    • Development and optimization experiments
    • System suitability controls
    • Preliminary specificity
    • Preliminary precision experiments
    • Sample suitability
  • Stage 4. Performance qualification (Documented experiments demonstrating that a specific process will meet its pre-determined acceptance criteria).
    • Calibration curve
    • Range
    • Linearity or non-linear regression (Logistic models)
    • Intra-Precision
    • Inter-Precision
    • Limit of quantitation
    • Limit of Detection
    • Accuracy
    • Specificity
  • Stage 5. Performance validation (Documented testing which demonstrates that a process consistently operates according to predetermined (qualified) acceptance criteria).
    • Reproducibility
    • Stability
      • Freeze and Thaw Stability
      • Short-Term Temperature Stability
      • Long-Term Temperature Stability
      • Stock Solution Stability
    • Robustness
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Elisa Kit Development
Starting at $5,000.00 per unit

Established Assay Development

MoonElisa can use private label for the developed assay in kit format so it can be marketed by your organization.

MoonElisa Biotech will provide Contract ELISA Development services for the R&D laboratories and industrial companies. We are providing custom service that will meet your... Show more »

Established Assay Development

MoonElisa can use private label for the developed assay in kit format so it can be marketed by your organization.

MoonElisa Biotech will provide Contract ELISA Development services for the R&D laboratories and industrial companies. We are providing custom service that will meet your customer specific needs. If you need to detect or measure a specific target molecule, we can fully develop a quick and accurate immunoassay test or optimize an existing assay according to your specifications.

Immunoassay development begins with consultation with our scientific team. Method development feasibility is determined based on the availability of assay components and reagents with the desired assay specific parameters. We can also help with reviewing publications, design of experiment, statistical analysis, and sourcing reagents.

Assay development typically costs between $2,000 - $10,000 and usually takes 6-12 weeks. The cost of kit manufacturing depends on the composition of the reagents. If the assay has a commercial application, MoonELISA can use your private label for the developed assay in kit format so it can be marketed by your organization. Specific labels or packaging may be requested for sourcing or supplied by the customer for use in the final product.

  • Based on your request and specific needs, assay development begins with consultation with our scientific team.
  • Method development feasibility is determined based on the availability of assay components and reagents with the desired assay specific parameters. MoonElisa generally charges $75 an hour for consultation, reviewing publications, design of experiment, statistical analysis, and sourcing reagents".
  • For consultation projects, MoonElisa can provide a quote of estimated time and expenses and will discuss a specific payment schedule with each customer. MoonElisa will email you a detailed quote for your specific assay development project including our feasibility study for your request. MoonElisa generally charges $75 an hour for quote preparation and conducting feasibility study for your product".
  • After signing the approval agreement with us, you initiate a request for our ELISA Method Development.
  • A comprehensive protocol for this custom ELISA will be provided at the end of our service.
  • We will provide ongoing manufacturing services for the test components according to the client's demand.
  • For any particular reason, If we fail to set up the ELISA assay for your antigen, we will refund 75% of the cost of assay development (minus 25% cost for consumables).

New Assay Development

If you can't find an affordable ELISA Kit for your protein of interest, or having trouble creating your own in-house assay kits, or looking for a cheaper source for your ELISA kit manufacturing, Let us assist you by using private labeling for any developed assay in akit format so it can be used by your organization.

MoonElisa Biotech provides "New ELISA Development service" for R&D laboratories and industrial companies. If you need to detect or measure a specific targeted molecule, we fully develop a quick and accurate immunoassay test or even optimize an existing assay based on your provided specifications. Our Immunoassay development simply begins with a consultation with our scientific team. Then, a Method development feasibility is determined based on the availability of assay components and reagents with the desired assay specific parameters. We can also assist with reviewing publications, design of experiment, statistical analysis, and sourcing reagents.

If the target analyte and/or necessary antibodies are not available, Vector design, antigen expression, antibody production, antibody labeling, and ELISA assay development typically cost between $50,000 - $250,000 and usually takes 8-12 months.

If the assay has a commercial application, MoonELISA can use your private label for the developed assay in kit format so it can be marketed by your organization. Specific labels or packaging may be requested for sourcing or supplied by the customer for use in the final product.

  • Based on your request and specific needs, assay development begins with consultation with our scientific team.
  • Method development feasibility is determined based on the availability of assay components and reagents with the desired assay specific parameters. MoonElisa generally charges $75 an hour for consultation, reviewing publications, design of experiment, statistical analysis, and sourcing reagents".
  • MoonElisa provide a quote of estimated time and expenses and will discuss a specific payment schedule with each customer. MoonElisa will email you a detailed quote for your specific assay development project including our feasibility study for your request. MoonElisa generally charges $75 an hour for quote preparation and conducting feasibility study for your product".
  • After signing the approval of agreements with us, you initiate a request for New Assay ELISA Method Development.
  • A comprehensive protocol for this custom ELISA will be provided at the end of our service.
  • We will provide ongoing manufacturing services for the test components according to the client's demand.

Payments are typically arranged with a front fee of 50% of the estimated total, and payments installed thereafter, which can be paid monthly.

Once the assay is being developed, we will ship the kit components for your on-site testing. We will provide ongoing manufacturing services for the test components according to the client's demand. A comprehensive protocol for this custom ELISA will be provided at the end of our service. The cost for the kits typically ranges from $75 - $200 each. This price will be determined during assay development and depends on the expense of the assay kit components, the type of packaging, and the volume is being requested.

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Protein Identification
Starting at $3.00 per sample

ELISA Testing Services

"We know that ELISA is a time consuming assay. We want you to concentrate on something else while we are doing the hard work for you".

MoonElisa offers in-house testing services for protein identification and quantification. We offer a two working day turnaround time on results from the... Show more »

ELISA Testing Services

"We know that ELISA is a time consuming assay. We want you to concentrate on something else while we are doing the hard work for you".

MoonElisa offers in-house testing services for protein identification and quantification. We offer a two working day turnaround time on results from the time we receive a sample on most tests. Same day rush is available for samples received before 10:30am (Central). We will mail the original report to the listed contact person. Our testing services are highly confidential. Information is only reported to the requesting company and then only to their indicated contact persons.

Our laboratory offers full testing for all ELISA kits that were listed in our website. Customers can also send us their own antibodies and/or standards to be used in our ELISA testing services. If customers use their own antibodies, a discounted quotation will be applied. The testing will be conducted at our facility.

Please note that samples which are run at several dilutions or the re-running of samples at different dilutions will be considered as additional samples (40 samples/plate).

No. of Samples

  • > 61 samples: $179.0 /ELISA plate + $1.75/sample
  • 41-60 samples: $179.0 /ELISA plate + $3.0/sample
  • 31-40 samples $179.0 /ELISA plate + $5.0/sample
  • 17-30 samples : $179.0 /ELISA plate + $7.0/sample.
  • 1-16 samples: $115.0 /ELISA plate + $9.0/sample.

Samples must be free of infections or any hazardous materials, such as HIV or other communicable viruses. MoonElisa will store your samples appropriately for four weeks. Fees will be applied upon customer request after four weeks for any extended storage time. Please send your samples frozen or refrigerated.

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Antibody Array
Starting at $225.00 per plate

You can select up to 90 analytes in one 96 well plate to see them all at the same time. This service provides all necessary reagents for your research. You don't need to pay extra to buy more. A comprehensive protocol for this custom 96-well plate array will be provided at the end of our service.
MoonElisa Antibody Arrays... Show more »

You can select up to 90 analytes in one 96 well plate to see them all at the same time. This service provides all necessary reagents for your research. You don't need to pay extra to buy more. A comprehensive protocol for this custom 96-well plate array will be provided at the end of our service.
MoonElisa Antibody Arrays are microplate-based assays designed to detect the relative concentrations of multiple proteins in a single sample of cell lysate/media/or buffer. These assays utilize 96-well microplates that have up to 90 different capture antibodies spotted in each well. Additionally, our 96-well protein array can be purchased as either predefined or custom-ordered kits. Customers can also send us their own antibodies and/or standards to be used in our 96-well protein array assay, in which instance, a discounted quotation will be applied.

  • Based on your request and specific needs, protein array develpment will begin with a consultation with our scientific team.
  • Customize your own 96-well plates by choosing from the list of antibodies that are listed on our website.
  • Clients can provide their own antigens/antibodies or their own wish-list analytes to be customized in 96-well protein array format.
  • MoonElisa will provide a quote of estimated time and expenses and will discuss a specific payment schedule with each customer. MoonElisa will email you a detailed quote for your specific protein array development project including our feasibility study for your request. MoonElisa generally charges $75 an hour for consultation, feasability studies, design of experiment, statistical analysis, and sourcing reagents.
  • Assay development feasibility is determined based on the availability of assay components and reagents with the desired assay specific parameters.
  • After signing the approval agreement with us, MoonElisa will proceed with your protein-array development request.
  • A comprehensive protocol for this custom 96-well plate array will be provided at the end of our service.
  • We will provide ongoing ELISA manufacturing services for the test components according to the client's demand.

Pre-defined 96-well protein array format

You can choose one or more of the following pre-defined 96-well protein array designs:

Number of analytes:
1 (Quantitative) 1 capture antibody
2 (Quantitative) 2 capture antibodies
3 (Quantitative) 3 capture antibodies
4 (Quantitative) 4 capture antibodies
8 (Qualitative) 8 capture antibodies
16 (Qualitative) 16 capture antibodies
21 (Qualitative) 21 capture antibodies
42 (Qualitative) 42 capture antibodies

96 (Qualitative) 96capture antibodies

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Custom Microtiter Plate Coating
Starting at $22.00 per plate

MoonElisa can test your coated plates for antigen stability, antibody activity, and assay sensitivity for different periods of time such as 1, 3, 6, and 12 months.

If you need target protein or samples to be coated onto a microplate, we can do that for you. MoonElisa will coat, block, stabilize, dry, and seal them in insulted... Show more »

MoonElisa can test your coated plates for antigen stability, antibody activity, and assay sensitivity for different periods of time such as 1, 3, 6, and 12 months.

If you need target protein or samples to be coated onto a microplate, we can do that for you. MoonElisa will coat, block, stabilize, dry, and seal them in insulted foil bags with a desiccant packet so that they are ready to use any time you need to run a sample. MoonELISA can use private labels for the coated plates so that they can be marketed by your organization. We can also test your coated plates for protein stability and assay sensitivity for different periods of time such as 1, 3, 6, and 12 months.

Specific types of plates, blocking buffers, and reagent stabilizers can be provided upon customer request. Send us your target protein, antibody, or materials to be coated to the plate. We will get started as soon as we receive the materials, such as the reagent to be coated. Samples must be free of infections or any hazardous materials, such as HIV or other communicable viruses.

Most microplate coating projects can be finished within 3-4 days.

The typical cost for this service is $22-35/ plate.

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Biology
Price on request
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Omics
Price on request
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Proteomics
Price on request
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Protein Arrays
Price on request
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Cell-Based Assays
Price on request
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Specialized Cell-Based Assays
Price on request
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Biochemistry & Molecular Biology
Price on request
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Protein Services
Price on request
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Protein Sequencing
Price on request
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Protein Quantification
Price on request
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Product Development, Testing, and Packaging
Price on request
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Product Testing Services
Price on request
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Pharmacology & Toxicology
Price on request
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Immunoassays
Price on request
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