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Monoclonal Antibody Core Facility (DFCI)

9 Orders Completed
Boston, Massachusetts, US

About Monoclonal Antibody Core Facility (DFCI)

The Monoclonal Antibody Core at Dana-Farber Cancer Institute produces novel monoclonal antibodies for use in basic research, drug discovery, and clinical applications including diagnosis, surrogate markers for disease status, response to therapy, and drug toxicity. Services include cloning and scale-up of... Show more »

The Monoclonal Antibody Core at Dana-Farber Cancer Institute produces novel monoclonal antibodies for use in basic research, drug discovery, and clinical applications including diagnosis, surrogate markers for disease status, response to therapy, and drug toxicity. Services include cloning and scale-up of immunization antigens to purification of the resulting antibodies. Researchers can choose to have all phases of antibody production conducted by the core or perform particular steps in their own lab.

Services

  • Consultation on design of antigens and screening strategies
  • Production of vectors and protein with appropriate tags for immunization and screening
  • Immunization of animals including mice, rats and hamsters
  • Generation of monoclonal antibodies
  • Customized screening by WB, ELISA, flow cytometry
  • Antibody isotyping, titering, and specificity evaluation
  • Small, medium, and large-scale antibody production
  • Purification of antibodies
  • Antibody productivity assessment
  • Cryopreservation and recovery of hybridomas from liquid nitrogen
  • Hybridoma banking
  • Adaptation of hybridomas to serum-free media
  • Mycoplasma testing for hybridomas and other cell lines
  • Antibody affinity determination by Biacore 3000
  • CDR sequencing (variable region sequencing of mouse immunoglobulin heavy and light chains)

Available animal models and cell lines:
We use standard strains of mice, rats and rabbits. Investigators can provide us with knock-out and transgenic animals when they are available. We have a variety of hybridoma fusion partner lines available (SP 2/0; P3X, NS1, YB 2/0, YB 1,2,3, 240E-1).

Equipment:
Biosafety cabinets, CO2 incubators, Molecular Devices Spectromax plate reader, Titertek plate washer, AKTA FPLC systems, access to Biacore and flow cytometers.

Lab members:
Edward Greenfield, PhD (Director); Craig Bencsics (Senior Scientist), Mohan Brahmandam (Scientist) and Yuhua Nong (Scientist).

Most recent publication:
Antbodies: A Laboratory Manual (edited by Edward Greenfield) published by Cold Spring Harbor Press 2013.

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Our Services (36)


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Antibody Characterization

Starting at $1.00 per sample

The Core is experienced in post-hybridoma antibody development and validation procedures that include isotyping, determination of antibody specificity, determination of CDR sequences of variable H and L chains and determination of antibody affinity by Biacore. Our core offers highly competitive prices for several antibody... Show more »

The Core is experienced in post-hybridoma antibody development and validation procedures that include isotyping, determination of antibody specificity, determination of CDR sequences of variable H and L chains and determination of antibody affinity by Biacore. Our core offers highly competitive prices for several antibody characterization services and significant discounts for multiple antibodies characterized and analyzed in parallel.
 
We offer extremely fast isotype testing service for hybridoma culture supernatants and purified antibodies. We also perform custom antibody specificity determination assays such as ELISA (both Indirect and Capture), western blot and FACS. The MAC also offers antibody validation services for purified antibodies, hybridoma culture supernatants and serum samples from immunized animals as well as custom biomarker validation and assay development services. We make every effort to validate antibodies by testing various conditions for biomarker studies.

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Antibody Purification

Price on request
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Antigen Design

Price on request

Consultation on design of antigens and screening strategies

Consultation on design of antigens and screening strategies

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ELISA

Enzyme-linked immunosorbent assay
Starting at $260.00 per plate

Additional costs for antibodies, ELISA kits, lysates.

Additional costs for antibodies, ELISA kits, lysates.

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Fluorescence Activated Cell Sorting (FACS)

Fluorescence Activated Cell Sorting
Price on request
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FPLC

Fast Performance liquid chromatography
Price on request

Affinity chromatography (protein A and protein G)

We have an AKTA Express FPLC system.

Sample requirements depends on the type of antibody they need purified. We do both affinity (protein A and protein G) and size exclusion chromatography. We also can produce and purify recombinant proteins with Gst, His and Fc tags. ... Show more »

Affinity chromatography (protein A and protein G)

We have an AKTA Express FPLC system.

Sample requirements depends on the type of antibody they need purified. We do both affinity (protein A and protein G) and size exclusion chromatography. We also can produce and purify recombinant proteins with Gst, His and Fc tags.

Turnaround time will depend on the volume or amount of material they are looking for. On average, a 100 ml scale mAb production takes about 3 weeks and 1 liter scale takes about 5 weeks.

We can accommodate projects up to a gram of low endotoxin antibody.

Cost is dependent on production scale of the hybridoma or recombinant cell line.

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Flow Cytometry

Price on request
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Animal Immunization for Antibody Production

Price on request

Animal immunizations: standard, RIMMS, subtractive protocols

Animal immunizations: standard, RIMMS, subtractive protocols

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Mycoplasma Testing

Price on request
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Antibody Production Consulting

Price on request

Process Development describes the conversation we have with our clients to customize the steps that will be used in their specific project. This includes antigen design and production, animal species/strain selection and immunization protocol determination, assay selection and/or design, antibody characterization, hybridoma method... Show more »

Process Development describes the conversation we have with our clients to customize the steps that will be used in their specific project. This includes antigen design and production, animal species/strain selection and immunization protocol determination, assay selection and/or design, antibody characterization, hybridoma method selection and screening design, or something required that is unique.

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Recombinant Protein Expression

Price on request

Mammalian, baculovirus and bacterial systems

Mammalian, baculovirus and bacterial systems

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Size Exclusion Chromatography (SEC)

Price on request

We have an AKTA Express FPLC system.

Sample requirements depends on the type of antibody they need purified. We do both affinity (protein A and protein G) and size exclusion chromatography. We also can produce and purify recombinant proteins with Gst, His and Fc tags.

Turnaround time will depend on the volume or amount... Show more »

We have an AKTA Express FPLC system.

Sample requirements depends on the type of antibody they need purified. We do both affinity (protein A and protein G) and size exclusion chromatography. We also can produce and purify recombinant proteins with Gst, His and Fc tags.

Turnaround time will depend on the volume or amount of material they are looking for. On average, a 100 ml scale mAb production takes about 3 weeks and 1 liter scale takes about 5 weeks.

We can accommodate projects up to a gram of low endotoxin antibody.

Cost is dependent on production scale of the hybridoma or recombinant cell line.

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Western Blot

Starting at $350.00 per gel
Request a quote for more information about this service.

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Monoclonal Antibody (mAb) Production

Price on request

Hybridoma fusion: PEG-assisted and electrofusion

Hybridoma fusion: PEG-assisted and electrofusion

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Hamster

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Biochemistry & Molecular Biology

Price on request
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Project Management

Price on request
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Analytical Chemistry Services

Price on request
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Biology

Price on request
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Assay Development

Price on request
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Therapeutic Monoclonal Antibody (mAb) Development

Price on request
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Cytometry

Price on request
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Cryopreservation

Price on request
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Cells and Tissues

Price on request
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Biospecimens

Price on request
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Protein Quantification

Price on request
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Cryostorage and Cryoprocessing

Price on request
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Chromatography

Price on request
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Liquid Chromatography (LC)

Price on request
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Testing of Cell Lines

Price on request
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Separation/Purification Services

Price on request
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Protein Services

Price on request
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Biospecimen Management and Storage

Price on request
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Protein Expression Visualization

Price on request
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Protein Production

Price on request
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Antibody Services

Price on request
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Chemistry and Materials

Price on request
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Craig Bencsics

Senior Scientist | Molecular Biology
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  • Negative review for Western Blot:

    August 2016

    "we were disappointed in the work product of the MAb Core at DFCI. The Western Blot they generated did not isolate for the right band of interest. They blamed the lysate but that has nothing to do with searching and isolating the appropriate band for the target. Not sure what happened but I wouldn't be inclined to use them again."

  • Positive review for Western Blot:

    April 2013

    "Excellent. Provided high quality western blot service."

Monoclonal Antibody Core Facility (DFCI) has not received any endorsements.