Monoclonal Antibody Core Facility (DFCI)
About Monoclonal Antibody Core Facility (DFCI)
The Monoclonal Antibody Core at Dana-Farber Cancer Institute produces novel monoclonal antibodies for use in basic research, drug discovery, and clinical applications including diagnosis, surrogate markers for disease status, response to therapy, and drug toxicity. Services include cloning and scale-up of... Show more »
The Monoclonal Antibody Core at Dana-Farber Cancer Institute produces novel monoclonal antibodies for use in basic research, drug discovery, and clinical applications including diagnosis, surrogate markers for disease status, response to therapy, and drug toxicity. Services include cloning and scale-up of immunization antigens to purification of the resulting antibodies. Researchers can choose to have all phases of antibody production conducted by the core or perform particular steps in their own lab.
Services
- Consultation on design of antigens and screening strategies
- Production of vectors and protein with appropriate tags for immunization and screening
- Immunization of animals including mice, rats and hamsters
- Generation of monoclonal antibodies
- Customized screening by WB, ELISA, flow cytometry
- Antibody isotyping, titering, and specificity evaluation
- Small, medium, and large-scale antibody production
- Purification of antibodies
- Antibody productivity assessment
- Cryopreservation and recovery of hybridomas from liquid nitrogen
- Hybridoma banking
- Adaptation of hybridomas to serum-free media
- Mycoplasma testing for hybridomas and other cell lines
- Antibody affinity determination by Biacore 3000
- CDR sequencing (variable region sequencing of mouse immunoglobulin heavy and light chains)
Available animal models and cell lines:
We use standard strains of mice, rats and rabbits. Investigators can provide us with knock-out and transgenic animals when they are available. We have a variety of hybridoma fusion partner lines available (SP 2/0; P3X, NS1, YB 2/0, YB 1,2,3, 240E-1).
Equipment:
Biosafety cabinets, CO2 incubators, Molecular Devices Spectromax plate reader, Titertek plate washer, AKTA FPLC systems, access to Biacore and flow cytometers.
Lab members:
Edward Greenfield, PhD (Director); Craig Bencsics (Senior Scientist), Mohan Brahmandam (Scientist) and Yuhua Nong (Scientist).
Most recent publication:
Antbodies: A Laboratory Manual (edited by Edward Greenfield) published by Cold Spring Harbor Press 2013.
Our Services (36)
Antibody Characterization
The Core is experienced in post-hybridoma antibody development and validation procedures that include isotyping, determination of antibody specificity, determination of CDR sequences of variable H and L chains and determination of antibody affinity by Biacore. Our core offers highly competitive prices for several antibody... Show more »
The Core is experienced in post-hybridoma antibody development and validation procedures that include isotyping, determination of antibody specificity, determination of CDR sequences of variable H and L chains and determination of antibody affinity by Biacore. Our core offers highly competitive prices for several antibody characterization services and significant discounts for multiple antibodies characterized and analyzed in parallel.
We offer extremely fast isotype testing service for hybridoma culture supernatants and purified antibodies. We also perform custom antibody specificity determination assays such as ELISA (both Indirect and Capture), western blot and FACS. The MAC also offers antibody validation services for purified antibodies, hybridoma culture supernatants and serum samples from immunized animals as well as custom biomarker validation and assay development services. We make every effort to validate antibodies by testing various conditions for biomarker studies.
Antibody Purification
Antigen Design
Consultation on design of antigens and screening strategies
Consultation on design of antigens and screening strategies
ELISA
Enzyme-linked immunosorbent assay
Additional costs for antibodies, ELISA kits, lysates.
Additional costs for antibodies, ELISA kits, lysates.
FACS
Fluorescence Activated Cell Sorting
FPLC
Fast Performance liquid chromatography
Affinity chromatography (protein A and protein G)
We have an AKTA Express FPLC system.
Sample requirements depends on the type of antibody they need purified. We do both affinity (protein A and protein G) and size exclusion chromatography. We also can produce and purify recombinant proteins with Gst, His and Fc tags. ... Show more »
Affinity chromatography (protein A and protein G)
We have an AKTA Express FPLC system.
Sample requirements depends on the type of antibody they need purified. We do both affinity (protein A and protein G) and size exclusion chromatography. We also can produce and purify recombinant proteins with Gst, His and Fc tags.
Turnaround time will depend on the volume or amount of material they are looking for. On average, a 100 ml scale mAb production takes about 3 weeks and 1 liter scale takes about 5 weeks.
We can accommodate projects up to a gram of low endotoxin antibody.
Cost is dependent on production scale of the hybridoma or recombinant cell line.
Flow Cytometry
Animal Immunization for Antibody Production
Animal immunizations: standard, RIMMS, subtractive protocols
Animal immunizations: standard, RIMMS, subtractive protocols
Mycoplasma Testing
Antibody Production Consulting
Process Development describes the conversation we have with our clients to customize the steps that will be used in their specific project. This includes antigen design and production, animal species/strain selection and immunization protocol determination, assay selection and/or design, antibody characterization, hybridoma method... Show more »
Process Development describes the conversation we have with our clients to customize the steps that will be used in their specific project. This includes antigen design and production, animal species/strain selection and immunization protocol determination, assay selection and/or design, antibody characterization, hybridoma method selection and screening design, or something required that is unique.
Recombinant Protein Expression
Mammalian, baculovirus and bacterial systems
Mammalian, baculovirus and bacterial systems
Size Exclusion Chromatography (SEC)
We have an AKTA Express FPLC system.
Sample requirements depends on the type of antibody they need purified. We do both affinity (protein A and protein G) and size exclusion chromatography. We also can produce and purify recombinant proteins with Gst, His and Fc tags.
Turnaround time will depend on the volume or amount... Show more »
We have an AKTA Express FPLC system.
Sample requirements depends on the type of antibody they need purified. We do both affinity (protein A and protein G) and size exclusion chromatography. We also can produce and purify recombinant proteins with Gst, His and Fc tags.
Turnaround time will depend on the volume or amount of material they are looking for. On average, a 100 ml scale mAb production takes about 3 weeks and 1 liter scale takes about 5 weeks.
We can accommodate projects up to a gram of low endotoxin antibody.
Cost is dependent on production scale of the hybridoma or recombinant cell line.
Western Blot
Monoclonal Antibody (mAb) Production
Hybridoma fusion: PEG-assisted and electrofusion
Hybridoma fusion: PEG-assisted and electrofusion
Biochemistry & Molecular Biology
Project Management
Analytical Chemistry Services
Biology
Assay Development
Therapeutic Monoclonal Antibody (mAb) Development
Cytometry
Cryopreservation
Cells and Tissues
Biospecimens
Protein Quantification
Cryostorage and Cryoprocessing
Chromatography
Liquid Chromatography (LC)
Testing of Cell Lines
Separation/Purification Services
Protein Services
Biospecimen Management and Storage
Protein Expression Visualization
Protein Production
Antibody Services
Chemistry and Materials
Not finding what you're looking for?
Get info on this provider's capabilities without requesting a quote.Craig Bencsics
Senior Scientist | Molecular Biology
Edward Greenfield
Director
DFCI MAC members & recommendations.pdf
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Negative review for Western Blot:
August 2016
"we were disappointed in the work product of the MAb Core at DFCI. The Western Blot they generated did not isolate for the right band of interest. They blamed the lysate but that has nothing to do with searching and isolating the appropriate band for the target. Not sure what happened but I wouldn't be inclined to use them again."
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Positive review for Western Blot:
April 2013
"Excellent. Provided high quality western blot service."
Monoclonal Antibody Core Facility (DFCI) has not received any endorsements.