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Monash Antibody Technologies Facility

Clayton, Victoria, AU

Monash Antibody Technologies Facility (MATF) is a global source of cost effective, high quality, high affinity, custom monoclonal antibodies. Our team of highly skilled scientists employ well-established standard operating procedures, along with the latest robotic techniques, to deliver successful projects to customers in a typical time frame of 12-16 weeks. We are known for our ability to succeed at challenging projects and strive to be the best at producing monoclonal antibodies where others have failed.

MATF has a wealth of experience in other areas such as robotics, high throughput screening, antibody purification and characterization and are keen to help customers with antibody or laboratory automation challenges.

MATF will work with the customer at every step to ensure the needs of each individual project are met and is proud to follow a Quality Management System that is certified to... Show more »

Monash Antibody Technologies Facility (MATF) is a global source of cost effective, high quality, high affinity, custom monoclonal antibodies. Our team of highly skilled scientists employ well-established standard operating procedures, along with the latest robotic techniques, to deliver successful projects to customers in a typical time frame of 12-16 weeks. We are known for our ability to succeed at challenging projects and strive to be the best at producing monoclonal antibodies where others have failed.

MATF has a wealth of experience in other areas such as robotics, high throughput screening, antibody purification and characterization and are keen to help customers with antibody or laboratory automation challenges.

MATF will work with the customer at every step to ensure the needs of each individual project are met and is proud to follow a Quality Management System that is certified to ISO9001.

Publications

  • A fully human inhibitory monoclonal antibody to the Wnt receptor RYK. Halford MM, Macheda ML, Parish CL, Takano EA, Fox S, Layton D, Nice E, Stacker SA. PLoS One. 2013 Sep 18;8(9): e75447. doi:10.1371/journal.pone.0075447.
  • A chromosome-centric human proteome project (C-HPP) to characterize the sets of proteins encoded in chromosome 17. Liu S, Im H, Bairoch A, Cristofanilli M, Chen R, Deutsch EW, Dalton S, Fenyo D, Fanayan S, Gates C, Gaudet P, Hincapie M, Hanash S, Kim H, Jeong SK, Lundberg E, Mias G, Menon R, Mu Z, Nice E, Paik YK, Uhlen M, Wells L, Wu SL, Yan F, Zhang F, Zhang Y, Snyder M, Omenn GS, Beavis RC, Hancock WS.
    J Proteome Res. 2013 Jan 4;12(1):45-57. doi: 10.1021/pr300985j.
  • Design and operation of an automated high-throughput monoclonal antibody facility. Layton D, Laverty C, Nice E. Biophys Rev. 2012 October 5 (1); 47-55. doi: 10.1007/s12551-012-0095-6.
  • A roadmap to generate renewable protein binders to the human proteome. Colwill K, Renewable Protein Binder Working Group, Gräslund S. Nat Methods. 2011 May 15;8(7): 551-8. doi: 10.1038/nmeth.1607.
  • High throughput production of mouse monoclonal antibodies using antigen microarrays. De Masi F, Chiarella P, Wilhelm H, Massimi M, Bullard B, Ansorge W, Sawyer A. Proteomics. 2005 Nov; 5(16): 4070-81.
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Elisa Kit Development
Price on request

Using Surface Plasmon Resonance Imaging (SPRi) technology, MATF is able to identify mAbs that bind in a non-competing manner with an antigen suitable for sandwich ELISA development. We can optimise the concentration of each antibody using our high-throughput robotic ELISA platforms. Using the ELISA platform ensures accurate and... Show more »

Using Surface Plasmon Resonance Imaging (SPRi) technology, MATF is able to identify mAbs that bind in a non-competing manner with an antigen suitable for sandwich ELISA development. We can optimise the concentration of each antibody using our high-throughput robotic ELISA platforms. Using the ELISA platform ensures accurate and consistent assessment of the antibody pairs every time.

If you don’t have complementary pairs of antibodies for your ELISA project, MATF can employ the custom antibody service to generate one or both of the antibodies needed.

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Antibody Purification
Price on request

We can generate between 1-500mg purified monoclonal antibody from hybridomas generated at MATF or those external to the facility once the lack of mycoplasma has been confirmed.

We can generate between 1-500mg purified monoclonal antibody from hybridomas generated at MATF or those external to the facility once the lack of mycoplasma has been confirmed.

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Therapeutic Monoclonal Antibody (mAb) Development
Price on request

Phosphor-specific antibody production:

Antibodies directed at phosphorylations sites are critical tools for understanding regulatory mechanisms that control cell signaling, transcription and enzyme activation. MATF can design and produce a phosphorylated and non-phosphorylated peptide and, using our proprietary screening,... Show more »

Phosphor-specific antibody production:

Antibodies directed at phosphorylations sites are critical tools for understanding regulatory mechanisms that control cell signaling, transcription and enzyme activation. MATF can design and produce a phosphorylated and non-phosphorylated peptide and, using our proprietary screening, find binders that specifically recognise the phosphorylation site of interest.

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Custom Monoclonal Antibody (mAb) Production
Price on request

MATF produces hybridoma cell lines expressing monoclonal antibodies against a custom target protein or peptide. The proprietary antigen microarray screening technology selects for large numbers of specific, high affinity, IgG secreting clones. MATF can perform a custom monoclonal production project with as little as 0.4mg of... Show more »

MATF produces hybridoma cell lines expressing monoclonal antibodies against a custom target protein or peptide. The proprietary antigen microarray screening technology selects for large numbers of specific, high affinity, IgG secreting clones. MATF can perform a custom monoclonal production project with as little as 0.4mg of protein or 1mg of peptide. MATF will deliver up to five individual clones and antibody supernatants with a typical production time of 12–16 weeks. Additional services and clones can be supplied on request.

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Antibody Services
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Biochemistry & Molecular Biology
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Protein Quantification
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Protein Services
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Biology
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