Molecular Pathology Core

OCT block. Services are also available for sucrose cryoprotection and making frozen blocks.

Brightfield slides, 20x scan, 1 month web-server storage time followed by transfer to your device.

Whole digital slide scanning allows online viewing of your entire section with up to 40x zoom from an original scan using a 20x objective. The current instrument is for brightfield scanning. The system focuses several points over the entire tissue providing unprecedented detail. Slide scanning is an excellent means to store slides, share with colleagues, or create publication quality snap-shots. Slide scanning can replace hours photographing regions on a microscope. Several image analysis programs are available from nucleus and membrane staining to color colocalization and deconvolution macros. Server image storage is available for 1 month and transfer to your storage media (server, DVD, USB) is required unless prior arrangements made.

Paraffin or OCT block per slide

per section

Single IHC/IF $24.00 per section
Double IHC/IF $38.50 per section
Triple IHC/IF $60 per section

Deliverables:

• images
• image analysis data (if requested analysis)
• presented in Microsoft power point until unless requested by client in any other method

Capabilities:

• preparation and mounting of sectioned tissue from fixed tissues
• preparation of both paraffin-embedded and frozen sections
• have access to a tissue bank, only for antibody validation purpose
• have access to FFPE tissues in-house: human, mouse almost all type of tissues
• we're happy to work with any cells that are sent to us

Data analyses available:

• Fluorescence quantitation

We do not:

• culture mammalian cells and prepare them for antibody staining
• culture non-mammalian (i.e. insect) cells
• transfect cells with either viruses or chemicals
• imaging of live cells

Equipment:

• Epifluorescence

Objective lenses:

• x5, x10, x20, x40, x63

Fluorescent channels and wavelengths:

• single channel for blue (DAPI), green (FITC, GFP) and red (AF555) and red (AF594) and Far-red (AF647)
• Dual channel and triple channel

The Immunohistochemistry (IHC) lab provides in-depth consultations including antibody selection, training, and services for established assays as well as testing new antibodies.

Includes primary antibodies when in stock. Per section prices.

ISH can be performed with any probe type supplied by user along with detection kit.

Process and embed, per cassette.

Cut 1 tissue section/slide, Superfrost+ slides

Consultations on small animal histopathology for Core users.

Routine: PAS/PASD, Oil red O (frozen only), Van Gieson’s, Congo Red, Masson’s Trichrome,
Complex: inquire
$15-$20/slide

Tissue microarray (TMA) capabilities from processing samples into donor blocks and evaluating regions of interest to TMA design, construction, sectioning, and staining. Any stain that can be used on a paraffin section can be performed on a TMA slide. Fees for TMA construction are based on technical support time and supplies. Individuals can also be trained to construct their own arrays.