The Molecular Pathology Core, Department of Pathology, College of Medicine, University of Florida, provides high quality histology, immunolocalization, and microscopy services for basic science and clinical research. Consultations and assistance are available for any phase from project design to specimen analysis. Preclinical studies are performed in compliance with GLP.
OCT block. Services are also available for sucrose cryoprotection and making frozen blocks.
Brightfield slides, 20x scan, 1 month web-server storage time followed by transfer to your device.
Whole digital slide scanning allows online viewing of your entire section with up to 40x zoom from an original scan using a 20x objective. The current instrument is for brightfield scanning. The system focuses several points over the entire tissue providing unprecedented detail. Slide scanning is an excellent means to store slides, share with colleagues, or create publication quality snap-shots. Slide scanning can replace hours photographing regions on a microscope. Several image analysis programs are available from nucleus and membrane staining to color colocalization and deconvolution macros. Server image storage is available for 1 month and transfer to your storage media (server, DVD, USB) is required unless prior arrangements made.
Paraffin or OCT block per slide
per section
Single IHC/IF $24.00 per section
Double IHC/IF $38.50 per section
Triple IHC/IF $60 per section
Deliverables:
Capabilities:
Data analyses available:
We do not:
Equipment:
Objective lenses:
Fluorescent channels and wavelengths:
The Immunohistochemistry (IHC) lab provides in-depth consultations including antibody selection, training, and services for established assays as well as testing new antibodies.
Includes primary antibodies when in stock. Per section prices.
ISH can be performed with any probe type supplied by user along with detection kit.
Process and embed, per cassette.
Cut 1 tissue section/slide, Superfrost+ slides
Consultations on small animal histopathology for Core users.
Routine: PAS/PASD, Oil red O (frozen only), Van Gieson’s, Congo Red, Masson’s Trichrome,
Complex: inquire
$15-$20/slide
Tissue microarray (TMA) capabilities from processing samples into donor blocks and evaluating regions of interest to TMA design, construction, sectioning, and staining. Any stain that can be used on a paraffin section can be performed on a TMA slide. Fees for TMA construction are based on technical support time and supplies. Individuals can also be trained to construct their own arrays.
"Nicely communicative"
"Great work conducting IF antibody validation"
Molecular Pathology Core has not received any endorsements.