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Molecular Pathology Core

100% Positive
10 Orders Completed
Gainesville, Florida, US

About Molecular Pathology Core

Divison of: University of Florida

The Molecular Pathology Core, Department of Pathology, College of Medicine, University of Florida, provides high quality histology, immunolocalization, and microscopy services for basic science and clinical research. Consultations and assistance are available for any phase from project design to specimen analysis. Preclinical studies are performed in compliance with GLP.

Our Services (32)


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Cryosectioning

Starting at $6.00 per slide

OCT block. Services are also available for sucrose cryoprotection and making frozen blocks.

OCT block. Services are also available for sucrose cryoprotection and making frozen blocks.

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Whole Slide Imaging/Digital Slide Scanning

Starting at $7.00 per sample

Brightfield slides, 20x scan, 1 month web-server storage time followed by transfer to your device.

Whole digital slide scanning allows online viewing of your entire section with up to 40x zoom from an original scan using a 20x objective. The current instrument is for brightfield scanning. The system focuses several points over... Show more »

Brightfield slides, 20x scan, 1 month web-server storage time followed by transfer to your device.

Whole digital slide scanning allows online viewing of your entire section with up to 40x zoom from an original scan using a 20x objective. The current instrument is for brightfield scanning. The system focuses several points over the entire tissue providing unprecedented detail. Slide scanning is an excellent means to store slides, share with colleagues, or create publication quality snap-shots. Slide scanning can replace hours photographing regions on a microscope. Several image analysis programs are available from nucleus and membrane staining to color colocalization and deconvolution macros. Server image storage is available for 1 month and transfer to your storage media (server, DVD, USB) is required unless prior arrangements made.

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H&E Staining

Hematoxylin and eosin staining
Starting at $3.00 per slide

Paraffin or OCT block per slide

Paraffin or OCT block per slide

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Immunofluorescence

Starting at $24.00 per slide

per section

Single IHC/IF $24.00 per section
Double IHC/IF $38.50 per section
Triple IHC/IF $60 per section

Deliverables:

  • images
  • image analysis data (if requested analysis)
  • presented in Microsoft power point until unless requested by client in any other method

Capabilities:

per section

Single IHC/IF $24.00 per section
Double IHC/IF $38.50 per section
Triple IHC/IF $60 per section

Deliverables:

  • images
  • image analysis data (if requested analysis)
  • presented in Microsoft power point until unless requested by client in any other method

Capabilities:

  • preparation and mounting of sectioned tissue from fixed tissues
  • preparation of both paraffin-embedded and frozen sections
  • have access to a tissue bank, only for antibody validation purpose
  • have access to FFPE tissues in-house: human, mouse almost all type of tissues
  • we're happy to work with any cells that are sent to us

Data analyses available:

  • Fluorescence quantitation

We do not:

  • culture mammalian cells and prepare them for antibody staining
  • culture non-mammalian (i.e. insect) cells
  • transfect cells with either viruses or chemicals
  • imaging of live cells

Equipment:

  • Epifluorescence

Objective lenses:

  • x5, x10, x20, x40, x63

Fluorescent channels and wavelengths:

  • single channel for blue (DAPI), green (FITC, GFP) and red (AF555) and red (AF594) and Far-red (AF647)
  • Dual channel and triple channel
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Immunohistochemistry (IHC)

Starting at $27.50 per sample

The Immunohistochemistry (IHC) lab provides in-depth consultations including antibody selection, training, and services for established assays as well as testing new antibodies.

Includes primary antibodies when in stock. Per section prices.

The Immunohistochemistry (IHC) lab provides in-depth consultations including antibody selection, training, and services for established assays as well as testing new antibodies.

Includes primary antibodies when in stock. Per section prices.

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Agilent DAKO

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In situ Hybridization (ISH)

In Situ Hybridization
Starting at $55.00 per hour

ISH can be performed with any probe type supplied by user along with detection kit.

ISH can be performed with any probe type supplied by user along with detection kit.

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Paraffin Embedding

Starting at $4.50 per sample

Process and embed, per cassette.

Process and embed, per cassette.

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Paraffin Sectioning

Starting at $4.40 per slide

Cut 1 tissue section/slide, Superfrost+ slides

Cut 1 tissue section/slide, Superfrost+ slides

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Veterinary Pathology

Price on request

Consultations on small animal histopathology for Core users.

Consultations on small animal histopathology for Core users.

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Special Slide Staining

Starting at $15.00 per slide

Routine: PAS/PASD, Oil red O (frozen only), Van Gieson’s, Congo Red, Masson’s Trichrome,
Complex: inquire
$15-$20/slide

Routine: PAS/PASD, Oil red O (frozen only), Van Gieson’s, Congo Red, Masson’s Trichrome,
Complex: inquire
$15-$20/slide

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Tissue Microarrays

Price on request

Tissue microarray (TMA) capabilities from processing samples into donor blocks and evaluating regions of interest to TMA design, construction, sectioning, and staining. Any stain that can be used on a paraffin section can be performed on a TMA slide. Fees for TMA construction are based on technical support time and supplies. Individuals can also be trained to construct their own arrays.

Tissue microarray (TMA) capabilities from processing samples into donor blocks and evaluating regions of interest to TMA design, construction, sectioning, and staining. Any stain that can be used on a paraffin section can be performed on a TMA slide. Fees for TMA construction are based on technical support time and supplies. Individuals can also be trained to construct their own arrays.

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Nucleic Acid Services

Price on request
Request a quote for more information about this service.

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Veterinary Laboratory Services

Price on request
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Clinical and Anatomic Pathology

Price on request
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Tissue Embedding

Price on request
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DNA Hybridization

Price on request
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Veterinary Research & Diagnostics

Price on request
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Protein Services

Price on request
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Common Histochemical Stains

Price on request
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Immunostaining

Price on request
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Protein Expression Visualization

Price on request
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Microscopy

Price on request
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Biochemistry & Molecular Biology

Price on request
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Histological Staining

Price on request
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DNA Services

Price on request
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Tissue Processing

Price on request
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Biology

Price on request
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Cells and Tissues

Price on request
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Imaging & Spectroscopy

Price on request
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Biospecimens

Price on request
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Clinical Research

Price on request
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Tissue Sectioning

Price on request
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