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MOgene, LC

St. Louis, Missouri, US

MOgene’s mission is to provide “best in class” services to its partners in deploying genomic capabilities to bring critical solutions to both agriculture and industrial biotech operations. In addition to being an Agilent Certified Service Provider MOgene is also a CLIA certified genomics service facility offering one stop service and solution from Tissue/Cells to Analysis. We offer RNA/DNA isolation, Microarrays, NextGen sequencing, Real time PCR and bioinformatics services. The founders of MOgene have extensive experience in developing insights regarding market needs and bringing innovative solutions to a wide range of sectors in life sciences, from agricultural, food, industrial markets to clinical studies, in commercial or academic institutions.

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DNA/RNA Analysis and Quality Control
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Agilent 2100 Bioanalyzer
SMRT Sequencing
Single Molecule Real-Time Sequencing
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Library preparation
Whole genome sequencing (bacteria, fungal, insects, plants etc)
IsoSeq
DNA capture

Library preparation
Whole genome sequencing (bacteria, fungal, insects, plants etc)
IsoSeq
DNA capture

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PacBio Sequel System
Agilent RNA microarray
Price on request

Agilent platform (Agilent-certified)

  • mRNA, miRNA , ChIP-onChIP, Methylation, and CGH arrays

Agilent platform (Agilent-certified)

  • mRNA, miRNA , ChIP-onChIP, Methylation, and CGH arrays
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Agilent
Agilent microRNA (miRNA) Microarray
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Agilent platform (Agilent-certified)

  • mRNA, miRNA , ChIP-onChIP, Methylation, and CGH arrays

Agilent platform (Agilent-certified)

  • mRNA, miRNA , ChIP-onChIP, Methylation, and CGH arrays
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Agilent
Bioinformatics
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  • de novo assembly
  • microarray analysis
  • transcriptome analysis
  • custom analysis
  • de novo assembly
  • microarray analysis
  • transcriptome analysis
  • custom analysis
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ChIP-ChIP
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Agilent's ChIP-on-chip (chromatin immunoprecipitation-on-chip) is the powerful technology driving this next generation microarray platform. ChIP-on-chip, also known as Location Analysis (LA), provides insight into key mechanisms of methylation, histone modification, as well as DNA replication, modification, and repair. It has... Show more »

Agilent's ChIP-on-chip (chromatin immunoprecipitation-on-chip) is the powerful technology driving this next generation microarray platform. ChIP-on-chip, also known as Location Analysis (LA), provides insight into key mechanisms of methylation, histone modification, as well as DNA replication, modification, and repair. It has been used to understand diseases such as diabetes, leukemia, and breast cancer, and has already provided important insight to vital processes like cell proliferation, cell fate determination, oncogenesis, cell cycle, apoptosis, and neurogenesis.


ChIP-on-chip pairs chromatin immunoprecipitation (ChIP) with glass slide microarrays (chip) to analyze how regulatory proteins interact with the genome of living cells. Regulatory proteins bind to genomic DNA to control chromosome replication and gene activity, thereby functioning as switches in the regulatory circuitry of cells. This network of circuits is uncharted in many instances and its understanding will aid researchers and companies in identifying new target genes and therapeutics capable of modulating these pathways. Combining the new information gained from ChIP-on-chip studies with the wealth of already available gene expression data will help speed and focus both disease research and drug discovery.


ChIP-on-chip technology enables the researcher to successfully focus on many critical areas previously hampered by the lack of useful tools to complete the studies.

It allows researchers to:

  • Uncover and validate gene regulation and regulatory networks by comprehensive determination of promoter occupancy.

  • Identify and characterize molecular events associated with processes for transcription, DNA replication and repair, as well as with chromatin modifications and DNA methylation.

  • Elucidate modes of action and potential therapeutic activities of compounds and target genes by mapping gene regulatory networks relevant to disease and pathophysiological states.

  • Validate and augment existing gene expression data with authentic binding events.

  • Identify, assess, and monitor biomarkers response to protein-DNA binding events to serve as bioassays or toxicant signatures for toxicogenomics.

  • Uncover and profile off-target events as well as validate primary and secondary effects in screening of candidate compounds, siRNAs, therapeutics, etc.


Comprehensive genome-wide and focused coverage:

Each Agilent ChIP-on-chip microarray features a total of ~244,000 60-mer oligonucleotide probes. Probes are spaced every ~~100-300 bp across regions of interest in both coding and non-coding DNA sequence. Available formats include whole genome, selected, and focused promoter regions. Such focused microarrays include Proximal Promoter (-0.8 KB upstream to +0.2 KB downstream of identified transcriptional start sites) and Expanded Promoter (-8.0 KB upstream to +2.0 KB downstream)designs.


The ChIP-on-chip Workflow consists of multiple steps. It begins with preparation of protein-DNA complexes from starting samples, followed by hybridization of the resulting labeled pool of DNA fragments to a ChIP-on-chip microarray for subsequent analysis. Major steps include:

  1. Rapid fixation of cells chemically cross-links DNA binding proteins to their genomic targets in vivo.

  2. Cell lysis releases the DNA-protein complexes, and sonication fragments the DNA.

  3. Immunoprecipitation (IP) purifies the protein-DNA fragments, with specificity dictated by antibody choice.

  4. Hydrolysis reverses the cross-links within the released DNA fragments, and amplification is performed.

  5. Labeling pool of protein-DNA fragments.

  6. Hybridization of DNA onto Agilent-validated or user-customized microarrays featuring 60-mer oligonucleotide probes.

  7. Data analysis with a high-resolution array scanning to detect significant binding events and results comparison with gene annotation databases using Agilent ChIP Analytics software (see following section).

The ChIP-on-chip process enables the observation of a few to 50-fold the representation of protein-bound (occupied) DNA promoter fragments of interest in the final DNA pool.

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Agilent
Targeted Sequencing
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Targeted amplification/Sequence Capture:

  • Fluidigm Access Array
  • Agilent SureSelect & HaloPlex
  • NimbleGen SeqCap

NimbleGen's Sequence Capture arrays can capture up to 30Mb (2.1M) or 5Mb (385K) genomic regions as enriched DNA fragments and can be sequenced to the researchers desired depth and % base coverage... Show more »

Targeted amplification/Sequence Capture:

  • Fluidigm Access Array
  • Agilent SureSelect & HaloPlex
  • NimbleGen SeqCap

NimbleGen's Sequence Capture arrays can capture up to 30Mb (2.1M) or 5Mb (385K) genomic regions as enriched DNA fragments and can be sequenced to the researchers desired depth and % base coverage using the GS FLX Titanium series reagents. In addition, the 2.1M Human Exome arrays are capable of capturing ~180,000 human coding exons and ~550 miRNA exons (from the CCDS database, build April 30th, 2008) on a single array. Benefit from GS FLX Titanium long reads for haplotyping and straightforward identification of insertions and deletions, without GC bias.

Data Analysis:
- GS Mapper Software

How it works:
- The genomic DNA sample is fragmented and polished
- The linkers are ligated to the fragments
- The sample is hybridized to a NimbleGen Sequence Capture array
- The unbound fragments are washed away
- The target fragments are eluted using the NimbleGen Elution System
- The target enriched pool is amplified
- The enriched, amplified pool is then verified by qPCR
- The enriched sample is then ready for high-throughput sequencing with the Genome Sequencer FLX by 454 Life Sciences

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Agilent SureSelect
Agilent HaloPlex
Fluidigm Access Array
NimbleGen SeqCap
Fluidigm access array
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Key Applications: Targeted Resequencing

The Access Array System is the first high-throughput, target-enrichment system designed to work with Roche-454 FLX and all other major next-generation sequencing instruments. The Access Array System enables the user to enrich multiple unique targets (such as exons) from a large number of... Show more »

Key Applications: Targeted Resequencing

The Access Array System is the first high-throughput, target-enrichment system designed to work with Roche-454 FLX and all other major next-generation sequencing instruments. The Access Array System enables the user to enrich multiple unique targets (such as exons) from a large number of samples, all at one time. The system combines the cost and throughput benefits of microfluidics with the proven performance and flexibility of PCR.

Features include:

  • Target Enrichment
  • Catalog and Custom Combined PCR primer and Sample Barcoding for Multiplexed Sequencing
  • Up to 48 Simultaneous Sequencing Library Preps Using Amplicon Tagging
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Genomic DNA Extraction
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RNA/DNA isolation from cells, tissues, blood, buccal swabs etc.


Standard RNA Isolation Services:
Total RNA for microarrays (gene expression and miRNA) or for QPCR
1. Cells: transformed, cultured cells or primary cells
2. Tissue Samples or Biopsies: fresh or frozen
3. Blood
4. Tissues embedded... Show more »

RNA/DNA isolation from cells, tissues, blood, buccal swabs etc.


Standard RNA Isolation Services:
Total RNA for microarrays (gene expression and miRNA) or for QPCR
1. Cells: transformed, cultured cells or primary cells
2. Tissue Samples or Biopsies: fresh or frozen
3. Blood
4. Tissues embedded in OCT
5. Others: Please contact Technical Support to discuss your needs.


All RNA Isolation Services include:
- Confirmation of RNA sample concentration on a NanoDrop spectrophotometer and RNA quality on an Agilent 2100 BioAnalyzer with a RNA 6000 NanoChip Direct use in one our expression analysis services:
- RT² Real-Time PCR
- Agilent DNA
- Microarrays
- NimbleGen

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Illumina Sequencing
Price on request

Applications include:

  • Targeted Resequencing
  • Amplicon Sequencing - extremely high depth supports finding rare variants
  • Hybrid Capture - Targeted re-sequencing allows researchers to analyze a specific subset of the genome to discover and validate novel variants, examine specific genes in pathways, or as a follow-up to... Show more »

Applications include:

  • Targeted Resequencing
  • Amplicon Sequencing - extremely high depth supports finding rare variants
  • Hybrid Capture - Targeted re-sequencing allows researchers to analyze a specific subset of the genome to discover and validate novel variants, examine specific genes in pathways, or as a follow-up to GWAS data.
  • 16S Metagenomics - The 16s rRNA V4 region was sequenced using 2x150 overlapping reads with 24 indexed samples and used to survey the bacterial diversity in metagenomic samples.
  • Small Genome Sequencing
  • De novo Sequencing - MiSeq is ideal for sequencing small (< 20Mb) bacterial genomes with high copy number and high quality paired end reads.
  • Re-sequencing - MiSeq re-sequenced the 5.2 Mb Bacillus cerus genome yielding 175 Mb of data aligned to ATCC10987 with a mismatch rate of 0.06%. Its 5.4 million reads captures > 98% of the B. cereus genome with average coverage of 30x
  • Clone Checking - MiSeq is capable of performing fast analysis of plasimid constructs used to clone DNA. In less than a day, plasmid constructs can be sequenced, confirming the presence and integrity of the inserted piece of DNA.
  • Custom Amplicon - TruSeq Custom Amplicon allows sequencing of hundreds of genomic regions covering up to 96 kb of cumulative sequence. This highly targeted approach enables a wide range of applications for discovering, validating, and screening genetic variants in a rapid and efficient manner.
  • RNA Sequencing
  • RNA-Seq (microbial)
  • Small RNA Sequencing - Enables discovery and profiling of microRNAs and other non-coding RNA on any organism, without prior genome annotation. Using low RNA inputs, you can profile differential expression of known microRNAs and detect novel microRNA.
  • Library QC - a MiSeq run can be an economical surrogate to test library construction before committing time and resources to a HiSeq.
  • ChIP-Seq - Chromatin immunoprecipitation (ChIP) selectively finds DNA sequences bound by a particular protein. The ChIP process enriches specific crosslinked DNA protein complexes using an antibody and unique oligonucleotide adapters added to small stretches of DNA bound to the protein of interest
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NimbleGen Exon Sequencing
Price on request

NimbleGen Sequence Capture (SeqCap) arrays enable you to produce targeted, sequencing-ready samples in your lab for use with the Genome Sequencer FLX System and GS FLX Titanium Kits from 454 Life Sciences. Utilizing high-density, long-oligo NimbleGen arrays, either the human exome or the human genomic regions you specify are... Show more »

NimbleGen Sequence Capture (SeqCap) arrays enable you to produce targeted, sequencing-ready samples in your lab for use with the Genome Sequencer FLX System and GS FLX Titanium Kits from 454 Life Sciences. Utilizing high-density, long-oligo NimbleGen arrays, either the human exome or the human genomic regions you specify are hybridized and eluted, ready for subsequent amplification and sequencing. This array-based process offers significant speed and scalability advantages over current PCR-based methods for targeted enrichment.

To supply our customers with an affordable, high-quality solution, we have validated, and optimized protocols for obtaining enriched DNA that can be directly and easily integrated into the workflow of the 454 Genome Sequencer FLX instrument. The 454 Genome Sequencer FLX instrument with FLX Titanium Kits delivers read lengths of 400bp (500MB raw sequence per PTP) and is the most appropriate sequencing technology for the NimbleGen Sequence Capture solution.

Captured samples can be fed directly into the 454 Titanium Shot-gun library preparation protocol for addition of sequencing adapters.

Sequence your captured samples on the 454 GS FLX to generate high-confidence sequencing data using up to ~1,000,000 reads for roughly 500Mb of raw sequence data per picotiter plate.

400bp reads enable haplotyping of 400bp and identification of insertions/deletions from single to tens of base pairs.

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RNA Extraction
Price on request

RNA/DNA isolation from cells, tissues, blood, buccal swabs etc.


Standard RNA Isolation Services:

Total RNA for microarrays (gene expression and miRNA) or for QPCR
1. Cells: transformed, cultured cells or primary cells
2. Tissue Samples or Biopsies: fresh or frozen
3. Blood
4. Tissues embedded... Show more »

RNA/DNA isolation from cells, tissues, blood, buccal swabs etc.


Standard RNA Isolation Services:

Total RNA for microarrays (gene expression and miRNA) or for QPCR
1. Cells: transformed, cultured cells or primary cells
2. Tissue Samples or Biopsies: fresh or frozen
3. Blood
4. Tissues embedded in OCT
5. Others: Please contact Technical Support to discuss your needs.


All RNA Isolation Services include:

  • Confirmation of RNA sample concentration on a NanoDrop spectrophotometer and RNA quality on an Agilent 2100 BioAnalyzer with a RNA 6000 NanoChip Direct use in one our expression analysis services:
  • RT² Real-Time PCR
  • Agilent DNA
  • Microarrays
  • NimbleGen
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Array CGH
Microarray-Based Comparative Genomic Hybridization
Price on request

Agilent’s microarray-based comparative genomic hybridization (aCGH) technology is a powerful solution to your research in cancer and developmental disorders. Choose from our wide product offerings of whole genome and zoom-in 60-mer oligo CGH microarrays for human, mouse, and rat. Agilent’s CGH end-to-end solution consists of... Show more »

Agilent’s microarray-based comparative genomic hybridization (aCGH) technology is a powerful solution to your research in cancer and developmental disorders. Choose from our wide product offerings of whole genome and zoom-in 60-mer oligo CGH microarrays for human, mouse, and rat. Agilent’s CGH end-to-end solution consists of flexible microarray formats, optimized and easy-to-use protocol, high resolution microarray scanning, and powerful analytics software to give you the best quality results.


High-performance with uniform resolution

The Agilent SurePrint G3 Human High-Resolution Discovery Microarray Kit 1x1M is the next generation high-resolution microarray for whole genome DNA variation discovery. This array has an evenly tiled probe design selected from the in silico-validated CGH probe database. Unlike our catalog CGH and CNV microarrays, this array design is not a gene-biased or CNV-biased design.

  • 963,000+ human sequences represented
  • Probes annotated against UCSC hg18 (NCBI Build 36, March 2006)
  • 2.6 KB overall median probe spacing, 3 KB average probe spacing
  • 0.5 μg total genomic DNA input requirement
  • 1 x 1M slide format printed using Agilent's 60-mer SurePrint technology
  • 5 slides per kit. Each slide contains one 1M microarray
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Agilent CGH Microarray
qPCR
Quantitative PCR
Price on request

Real-time PCR remains one of the most sensitive and quantitative tools for gene expression used today. MOgene offers both custom and pre-optimized QPCR assays for an assortment of specific applications. We manage all aspects of the project from assay design, to nucleic acid isolation, to data analysis.

High-throughput Meets... Show more »

Real-time PCR remains one of the most sensitive and quantitative tools for gene expression used today. MOgene offers both custom and pre-optimized QPCR assays for an assortment of specific applications. We manage all aspects of the project from assay design, to nucleic acid isolation, to data analysis.

High-throughput Meets Customization

Utilizing ABI’s 7900HT and TaqMan® 384-well micro fluidic cards MOgene has the capability of running hundreds of real-time PCR reactions simultaneously. We require only small amounts of sample, to run 1 to 8 samples in parallel against 12 to 384 TaqMan® Gene Expression Assay targets.

Service Features and Options

  • QPCR primer and probe design
  • Nucleic acid isolation from tissue, cells, biotechnology products, etc.
  • Quantitation using a NanoDrop® ND-1000
  • Qualitative analysis by an Agilent BioAnalyzer®
  • Primer/Probe optimization
  • Samples assayed in duplicate to ensure reproducibility
  • Controls included on each thermocycling run
  • Data report with project design description
  • Statistical analysis upon request
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Genotyping Services
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Genotyping Services

Genotyping Services

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DNA Sequencing
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DNA Extraction and Purification
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MicroRNA (miRNA) Microarray
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RNA Microarray Platforms
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Targeted Gene Sequencing
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Computational Modeling
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Biology
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Biostatistics & Bioinformatics
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Equipment
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Protein-DNA Interaction Analysis
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Next Generation Sequencing (NGS)
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Chromatin Immunoprecipitation (ChIP) Assays
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Exome Sequencing Services
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Biomolecular Interaction Analysis
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PCR
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RNA Microarray
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DNA
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RNA
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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DNA Hybridization
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November 22, 2016

Positive review received for Agilent RNA microarray:

"very responsive and prompt. would definitely use their service again."

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