MLM Medical Labs is a CAP- and ISO15189-accredited, GLP-certified and CLIA-registered Central and Specialty Lab that is dedicated exclusively to clinical trials. We offer sample logistics and laboratory diagnostic service for all phases of clinical studies in compliance to Good Clinical Practice (GCP) and Good Clinical Laboratory Practice (GCLP).
MLM is located in Moenchengladbach, just minutes away from the Dusseldorf airport.
We offer an entire portfolio of service elements necessary to support clinical trials:
We have a staff of 41 employees and currently supports 80-100 phase I-IV studies simultaneously. We provide a very broad portfolio of biomarkers, which have been validated according to FDA and EMA guidelines. Analytical work is conducted according to Good Clinical Practice (GCP) and Good Clinical Laboratory Practice (GCLP). Pharmacokinetic and bioequivalence studies are conducted in line with Good Laboratory Practice (GLP).
Our clients are renowned CROs, biotech and global pharmaceutical companies based in Europe, Asia and the USA. MLM assists in daily laboratory service for phaseI/phase II CROs. MLM has successfully been involved as central lab in a series of multicenter studies that have been challenging in terms of analytics or logistics.
In order to fully support our clients, we offer an outstanding degree of flexibility, quality and promptness. This attitude has been instrumental to MLM's growth over the years.
Offering comprehensive biostatistical consulting services including analysis and review from study design to study readouts to optimize research needs.
MD Biosciences offers models to study the inflammatory components involved in lung inflammation and asthma including OVA-induced asthma, HDM-induced asthma, LPS-acute lung injury, and bleomycin-induced fibrosis.
in vitro, in vivo and ex vivo modeling services for efficacy determinations, PK/PD assessments, mechanism of action studies, compound screening, biomarker analysis and more!
MD Biosciences features a wide range of validated in vivo models of human disease to support preclinical drug efficacy tests, and we provide customizable disease-specific models in order to fit each study's needs.
Variety of preclinical animal models for pharmacology, efficacy and mechanism of action studies in addition to biomarker analysis
Our capabilities include support for projects at every stage in the development pipeline, from the initial biomarker identification, qualification and validation through to downstream clinical applications. Some services include identification of druggable targets, characterization of indicators of disease severity or stratification of patient populations.
plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones
Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, and passive cutaneous anaphylaxis are our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry.
Passive Cutaneous Anaphylaxis (PCA) is an immediate dermal response to an allergan-IgE interaction. It is based on a passively transferred anaphylactic reaction from antiserum. Assessments include dry extravasation (mm), absorbancy, PK blood analysis and biomarker analysis.
Delayed Type Hypersensitivity (DTH) model evaluates the cell-mediated immune response to repeated exposure of skin to oxazolone. This results in a DTH reaction involving CD4+, CD8+ T cells and macrophages, resulting in local skin inflammation characterized by an increased thickness of the skin.
FITC & DNCB-induced Contact Dermatitis Models. These models share many of the features of human contact dermatitis, both sensitization and challenge occurs epicutaneously.
IMQ-Induced Psoriasis Assay
This rapid model of psoriasis-like inflammation involves the IL-17/IL-23 axis. Imiquimod is a potent TLR7/8 ligand and potent immune modulator. Application of imiquimod on the back and ear of mice induces a psoriasis-like inflammation.
Following induction by the intranasal administration of LPS, leukocytes (mainly neutrophils) are recruited to the lung. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.
Acute DSS-induced colitis in mice and rats
Chronic DSS-induced colitis in mice
TNBS-induced colitis in mice and rats
Collagen induced arthritis (CIA) and adjuvant induced arthritis (AIA) are two commonly used models for this purpose. At MD Biosciences we offer our clients the novel and rapid collagen antibody-induced arthritis (CAIA) model as well.
LPS is used to induce sepsis in mice, setting off an immune response activating immune cells that excrete cytokines.
The murine OVA-induced asthma model is a widely used model that results in the characteristic features of asthma allowing the study and assessment of novel treatments.
he House Dust Mite (HDM) murine model of allergic asthma is used to assess therapies with potential indications in airway inflammation and/or pulmonary inflammation driven primarily by repeated exposure to allergens. Model includes Th2- associated cytokine response, eosinophilic infiltration into the lungs, airway hyper responsiveness (AHR) and epithelial tissue remodeling.
The LPS lung inflammation model is an acute lung inflammation model where researchers can observe the pulmonary response following exposure to bacteria. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.
We conduct metabolic and inflammation research, with diet-induced rat and mouse models, and including the following assessments: feed intake, body weight, insulin levels, glucose tolerance, blood pressure, feed preparing, and biomarkers.
Metabolic disease services include models for diabetes, renal, obesity and inflammatory bowel disease. Multiple induction methods may be available for each disease. Diabetes models may be induced with streptozotocin, the use of db/db or NOD mice. Inflammatory Bowel Disease (IBD) may be induced with TNBS or DSS.
Ovalbumin-induced allergic asthma is a widely used model to reproduce the airway eosinophilia, pulmonary inflammation and elevated IgE levels found during asthma.
The House Dust Mite (HDM) model demonstrates significant relevance for investigating the molecular mechanisms of allergic asthma pathogenesis.
LPS lung inflammation models are widely used to study host-response patterns in the pulmonary compartment.
Bleomycin induced lung injury model in mice is used for studying idiopathic pulmonary fibrosis (IPF).
Screening and mechanism of action studies using primary or immortalized cell lines. Our scientists development experience crosses a broad range of study targets. Customizing each study for sponsor relevance including chosen cell system, stimulant time point(s), and read-outs.
Dermal research with a host of preclinical and translational models. Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, as well as passive cutaneous anaphylaxis form the stable of our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry
We offer validated models of many inflammatory diseases including skin (IL-23 psoriasis, dermatitis, delayed type hyper-sensitivity, anaphylaxis), gastro-intestinal (TNBS or DSS induced IBD), respiratory (acute lung injury, asthma, fibrosis), joint (rheumatoid arthritis) and sepsis (LPS induced)
Nucleic acid extractions from multiple sample types (fresh, frozen & FFPE tissues, liquid biopsies, disaggregated tumor samples, etc)
Various cancer models and specializes in murine solid tumors for both subcutaneous and orthotopic models, with an emphasis on disease progression, biomarkers and immunophenotyping. Other models include metastatic, xenographic and syngeneic.
Bioanaylsis for all sample types across all major methodologies.
RNA extraction from a wide variety of sample formats
A large animal model of neuropathic and nociceptive pain for preclinical evaluation of analgesic or neuro-protective therapies. The sciatica model was developed in the pig, providing more clinically relevant data at the preclinical stages. The model is suitable for evaluating both efficacy and PK/PD in the same animal and can be used for systemic, local and cell therapies as well as slow release drugs and devices.
DSS and TNBS-induced; custom protocols available upon request, as are derivative model validations
Within our laboratory services, our pharmacology services include specialized diagnostic testing, clinical trial testing, GLP and GMP assessments of compounds/biologics and more! Our goal is to facilitate the advancement of diagnostic capabilities and improve analytical methods through application of rigorous biomarker-based strategies.
Multiparametric (16+ color) on the BD LSR Fortessa instrument
Cell Viability & Proliferation Assays Services
Cell Invasion & Migration Assays Services
Protein Expression Visualization Services
Clinical and Anatomic Pathology Services
Biostatistics & Bioinformatics Services
"MD Biosciences is an experienced lab with considerable knowledge and expertise. I found them very easy to work with. Communication was outstanding and I was very pleased by the quality of the work from protocol construction through in life study execution to the final report. I would absolutely use their services again."
"MD Biosciences worked with our small sample volumes. They provided comprehensive results in a clear and practical format. Highly recommended!"
MLM Medical Labs GmbH has not received any endorsements.