Mirna Analytics main focus is on precision medicine service to researchers in medicine and veterinary medicine for measurement of microRNA in body fluids, particularly plasma, or in cells. Mirna Analytics also has the knowledge ad equipment to perform RT-qPCR determinations of mRNA, or eRNA, or other non-coding RNAs. RNA extraction from various sources (plasma, cells, whole blood, tissues) is another service. We work with human and animal samples.
MicroRNAs are small molecules made for short- and long-distance communication between cells. Micro-RNAs control the life-span of mRNAs (messenger RNAs). Micro RNAs are very stable, particularly in plasma, and constitute a new class of biomarkers. The concentration of these microRNAs in body fluids can indicate risks or protection from disease; response to treatment and some microRNAs are also useful as disease markers.
M-RNAs (messenger RNA) are made in the process that produces proteins, and are measures of gene-transcription. E-RNAs (enhancer RNAs) are non-coding RNAs and newly recognized as important members of the molecular control of gene transcription. MicroRNAs are highly similar between species, including humans and many pet, agricultural and exotic animals. In contrast, most mRNAs are species specific.
While Mirna Analytics does not limit its service to a specific disease, we have special interest in the pulmonary vasculature and pulmonary hypertension, in humans and animals.
Mirna Analytics provides service that carefully moves your project through the alphabet: Advice regarding study design, Begin lab work by isolating RNA, Continue lab work by measuring RNA levels, Determine and interpret results.
The Mirna Analytics co-founders are Serhiy Pylawka, Ian Ladran and Gabriele Grunig. The Mirna Analytics team has received training in molecular sciences at the best academic and biotechnology institutions in the US including: Baker Institute of Animal Health, Cornell University, Ithaca; Columbia, Mount Sinai, Rockefeller (HHMI lab) Universities in New York City; UCSF, Salk Institute for Biological Studies, DNAX Research Institute, in California.
Recent publications by co-founders:
Mirkovic I, Pylawka S, Hudspeth AJ., 'Rearrangements between differentiating hair cells coordinate planar polarity and the establishment of mirror symmetry in lateral-line neuromasts', Biol Open. 2012 May 15;1(5):498-505. doi: 10.1242/bio.2012570. Epub 2012 Apr 12.
Storb U, Shen HM, Longerich S, Ratnam S, Tanaka A, Bozek G, Pylawka S., 'Targeting of AID to immunoglobulin genes', Adv Exp Med Biol. 2007;596:83-91.
Hartley BJ, Tran N, Ladran I, Reggio K, Brennand KJ, 'Dopaminergic differentiation of schizophrenia hiPSCs', Mol Psychiatry. 2015 Jan 27. doi: 10.1038/mp.2014.194. [Epub ahead of print]
Brennand K, Savas JN, Kim Y, Tran N, Simone A, Hashimoto-Torii K, Beaumont KG, Kim HJ, Topol A, Ladran I, Abdelrahim M, Matikainen-Ankney B, Chao SH, Mrksich M, Rakic P, Fang G, Zhang B, Yates JR 3rd, Gage FH, 'Phenotypic differences in hiPSC NPCs derived from patients with schizophrenia', Mol Psychiatry. 2015 Mar;20(3):361-8. doi: 10.1038/mp.2014.22. Epub 2014 Apr 1.
Park SH, Chen WC, Esmaeil N, Lucas B, Marsh LM, Reibman J, Grunig G, 'Interleukin 13- and interleukin 17A-induced pulmonary hypertension phenotype due to inhalation of antigen and fine particles from air pollution', Pulm Circ. 2014 Dec;4(4):654-68. doi: 10.1086/678511.
Riemekasten G, Kuebler WM, Schermuly R, Seyfarth HJ, Behr J, Grohe C, Hoeper MM, Olschewski A, Kwapiszewska G, Ulrich S, Voswinckel R, Weissmann N, Worth H, Viales RR, Pullamsetti SS, Grunig G, 'Pulmonary arterial hypertension--a disease of the immune system?', Dtsch Med Wochenschr. 2014 Dec;139 Suppl 4:S116-20. doi: 10.1055/s-0034-1387452. Epub 2014 Dec 9.
RT-qPCR for first pair of samples, per miRNA, per samle: $199
qPCR: costs as low as $20 per sample
SYBR green protocol is generally used
Taqman primers and probes on request, or if needed
Costs for study design: $100
miRNA Analytics LLC has not received any reviews.
miRNA Analytics LLC has not received any endorsements.