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Microscopy Services Laboratory

2 Orders Completed
Chapel Hill, North Carolina, US

About Microscopy Services Laboratory

We offer outstanding electron microscopy sample preparation services and imaging on research-grade transmission and scanning electron microscopes. Our staff have over 30 years of combined experience in the field. Several hundred publications have featured images from samples prepared and imaged in the... Show more »

We offer outstanding electron microscopy sample preparation services and imaging on research-grade transmission and scanning electron microscopes. Our staff have over 30 years of combined experience in the field. Several hundred publications have featured images from samples prepared and imaged in the laboratory. When you contact us, we will discuss your project in detail and provide an accurate timeline and quote. Among the sample preparation services we offer are embedding, ultrathin sections, negative staining immunoEM, critical point drying and sputter coating.

For researchers local to our geographical area (Chapel Hill, North Carolina) we offer full training and access to light microscopy and image analysis systems. In addition to standard widefield, confocal and live-cell options, we offer access to a LaVision BioTec UltraMicroscope II light-sheet microscope. This system is ideal for imaging large, cleared samples with cellular resolution, and is compatible with all published clearing methods (including those based on organic solvents).

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Our Services (22)


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Electron Microscopy Sample Preparation Services

Price on request
  • Optimization of sample preparation for YOUR research project.
  • TEM specimen preparation:
    • processing from fixed sample to embedded block
    • negative stain preparations
  • Thick sections: toluidine blue stained 0.5-1.0 µm survey sections mounted on a microscope
    slide
  • Ultrathin sections: stained 70-90nm sections... Show more »
  • Optimization of sample preparation for YOUR research project.
  • TEM specimen preparation:
    • processing from fixed sample to embedded block
    • negative stain preparations
  • Thick sections: toluidine blue stained 0.5-1.0 µm survey sections mounted on a microscope
    slide
  • Ultrathin sections: stained 70-90nm sections mounted on TEM grids
  • Immuno methods including immuno-gold and silver enhanced immuno-gold.
  • SEM specimen preparation: includes fixation, dehydration, critical point drying, mounting, and
    sputter coating
  • Critical point drying
  • Sputter coating
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Transmission Electron Microscopy (TEM)

Transmission Electron Microscopy services
Starting at $134.40 per hour

PRICE FOR NON PROFIT: $104/hour

  • Training and access to JEOL JEM 1230 and Zeiss LEO EM 910 transmission electron microscopes.
  • Advice on optimization of imaging conditions and image analysis.
  • We also provide consultation, sample preparation services and staff imaging of user-prepared samples (see corresponding service... Show more »

PRICE FOR NON PROFIT: $104/hour

  • Training and access to JEOL JEM 1230 and Zeiss LEO EM 910 transmission electron microscopes.
  • Advice on optimization of imaging conditions and image analysis.
  • We also provide consultation, sample preparation services and staff imaging of user-prepared samples (see corresponding service descriptions for prices and more information).

Technical details:

  • JEOL JEM 1230 TEM
    • 40 kV to 120 kV accelerating voltage, LaB6 filament
    • 0.2 nm lattice, 0.38nm point resolution, high contrast objective pole piece
    • 50X to 600,000X magnification range
    • 5-axis goniometer stage in X, Y, Z, +/- 45 degree tilt, 360˚rotation
    • Gatan Orius SC1000 CCD camera with Gatan Microscopy Suite 3.0 software
  • Zeiss LEO EM 910 TEM
    • 40 kV to 120 kV accelerating voltage, tungsten filament
    • 0.4 nm point resolution, high contrast OIS system
    • 50X to 500,000X magnification range
    • Eucentric goniometer stage in X, Y, Z, +/- 30 degree tilt
    • Gatan Orius SC1000 CCD camera with Digital Micrograph 2.3.1 software
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Scanning Electron Microscopy (SEM)

Scanning Electron Microscopy services
Starting at $134.40 per hour

PRICE FOR NON PROFIT: $104/hour

  • Training and access to Zeiss Supra 25 Field Emission Scanning Electron Microscope
  • Advice on optimization of imaging conditions and image analysis.
  • We also provide consultation, sample preparation services and staff imaging of user-prepared samples (see corresponding service descriptions for... Show more »

PRICE FOR NON PROFIT: $104/hour

  • Training and access to Zeiss Supra 25 Field Emission Scanning Electron Microscope
  • Advice on optimization of imaging conditions and image analysis.
  • We also provide consultation, sample preparation services and staff imaging of user-prepared samples (see corresponding service descriptions for prices and more information)

Technical details:

  • Zeiss Supra 25 Field Emission SEM
    • Resolution: 2 nanometers
    • Accelerating voltage: 1 kV to 30 kV
    • Magnification 20X – 1,000,000X
    • Fully computer controlled with digital image capture
    • Motorized goniometer stage will hold up to 8 samples
    • In-lens secondary electron detector
    • Standard T-E secondary electron detector
    • Backscatter detector
    • STEM detector
    • EDX detector
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Microscopy Consulting

Starting at $113.80 per hour

PRICE FOR NON PROFIT: $109/hour

  • Imaging of EM samples by highly-trained staff, with over 30 years of combined experience.
  • Consultation for EM projects: advice on sample preparation, analysis, etc.
    Note:
  • These services are charged in addition to any EM imaging time and sample preparation services.

PRICE FOR NON PROFIT: $109/hour

  • Imaging of EM samples by highly-trained staff, with over 30 years of combined experience.
  • Consultation for EM projects: advice on sample preparation, analysis, etc.
    Note:
  • These services are charged in addition to any EM imaging time and sample preparation services.
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Electron microscopy

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Light Sheet Microscopy

Starting at $101.40 per hour

PRICE FOR NON PROFIT: $79/hour

  • Training and access to Lavision Ultramicroscope II light-sheet system.
  • Advice on sample preparation, optimization of imaging conditions and image analysis.
  • Ideal for studying large, fixed, cleared samples with cellular resolution. It is compatible with all popular clearing methods, including... Show more »

PRICE FOR NON PROFIT: $79/hour

  • Training and access to Lavision Ultramicroscope II light-sheet system.
  • Advice on sample preparation, optimization of imaging conditions and image analysis.
  • Ideal for studying large, fixed, cleared samples with cellular resolution. It is compatible with all popular clearing methods, including those based on organic solvents. Examples of applications include tracing neural connections, studying maps of neural activity, studying vasculature, finding sparse populations of cells in large organs and many others.
  • We don’t provide:
    • Sample preparation services for light-sheet microscopy
    • Staff imaging of user-prepared samples

Technical details:
This system illuminates samples with a light-sheet that is approximately 5um-thick at its narrowest point in the Z dimension. To avoid shadowing artifacts and allow full imaging of thick samples, 3 angled light-sheets can illuminate the sample from each side. In addition, the light-sheet can be scanned horizontally in the X dimension to avoid a loss of Z resolution near the edges. The system comes equipped with five laser lines (488, 514, 561, 647, 785nm) and filter sets compatible with all common fluorophores from the green to far red regions of the spectrum. The system will be outfitted with a 1.3X/0.1 (coming in January) and a 4X/0.3 objective (currently available), as well as a 1X/2X magnification slider, for a total of 4 possible magnifications. The objectives have working distances of 9 and 5.5mm respectively, and the system can easily accommodate samples around 10mm in diameter; which is more than sufficient to image an entire adult mouse brain. These objectives and the sample mounting system are fully compatible with immersion media used in all clearing protocols, including those that require harsh organic solvents. The system has an automated stage that allows easy tiling of large specimens.

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Confocal Microscopy

Starting at $66.60 per hour

PRICE FOR NON PROFIT: $52/hour

  • Training and access to Zeiss LSM 700 and LSM 710 confocal microscopes.
  • Advice on sample preparation, optimization of imaging conditions and image analysis.
  • Typical applications
    • Multi-channel, tiled Z stacks (all common fluorophores are compatible)
    • Live-cell imaging with temperature,... Show more »

PRICE FOR NON PROFIT: $52/hour

  • Training and access to Zeiss LSM 700 and LSM 710 confocal microscopes.
  • Advice on sample preparation, optimization of imaging conditions and image analysis.
  • Typical applications
    • Multi-channel, tiled Z stacks (all common fluorophores are compatible)
    • Live-cell imaging with temperature, CO2 and humidity control
    • FRAP
    • FRET
  • We don’t provide:
    • Sample preparation services for confocal microscopy
    • Staff imaging of user-prepared samples

Technical details:

  1. Zeiss LSM 710 Spectral Confocal Laser Scanning Microscope
  • Capable of automated z-series, time-lapse, xyzt, and spectral image capture.
  • Inverted CLSM with DIC controlled by Zeiss ZEN 2011 software
  • Two conventional and one spectral fluorescence channel and one transmitted light channel
  • Motorized stage with Definite Focus for automatic focus drift correction
  • Zen software for linear unmixing, FRAP, FRET, Co-localization
  • Zeiss PECON incubation system for live cell imaging with temperature and CO2 control, compatible with 35mm dishes and chambered coverglass slides
  • Excitation lines:
    • 405 nm
    • 458 nm
    • 488 nm
    • 514 nm
    • 543 nm
    • 594 nm
    • 633 nm
  • Objective Lenses:
    • 5X/0.16 Plan Apo
    • 10X/0.45 Plan Apo
    • 20X/0.80 Plan Apo
    • 40X/0.95 Plan Apo
    • 25X/0.80 Plan Apo Multi Immersion
    • 40X/1.3 Oil Plan Neo
    • 63X/1.4 Oil Plan Apo
  1. Zeiss LSM 700 Confocal Laser Scanning Microscope
  • Capable of automated z-series, time-lapse, xyzt, and tiling.
  • Inverted CLSM with DIC controlled by Zeiss ZEN 2011 software
  • Two conventional fluorescence channels and one transmitted channel
  • Motorized stage with Definite Focus for automatic focus drift correctionZen software for FRAP, FRET, Co-localization, etc.
  • Bioptechs temperature control system for live cell imaging, compatible with 35mm Bioptechs 0400417B dishes.
  • Excitation lines:
    • 405 nm
    • 488 nm
    • 555 nm
    • 639 nm
  • Objective lenses:
    • 5X/0.16 Plan Apo
    • 10X/0.45 Plan Apo
    • 20X/0.80 Plan Apo
    • 40X/0.6 LD Plan Neo
    • 40X/1.4 Plan Apo Oil
    • 63X/1.4 Plan Apo Oil
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Zeiss LSM710 Zeiss LSM 710 confocal microscope Zeiss LSM700 ZEN Fluorescent labeling Immunofluorescence immunohistochemistry Fluorescent insitu hybridization Triple-stain Immunohistochemistry Dual-stain Immunohistochemistry Biology Show 11 more tags Show less

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Widefield Fluorescence Microscopy

Starting at $66.60 per hour

PRICE FOR NON PROFIT: $52/hour

  • Training and access to Olympus BX61 and IX81 upright and inverted fluorescent microscopes.
  • Advice on sample preparation, optimization of imaging conditions and image analysis.
  • Typical applications
    • Multi-channel images for most fluorophore combinations
    • Deconvolution of Z-stacks
      -... Show more »

PRICE FOR NON PROFIT: $52/hour

  • Training and access to Olympus BX61 and IX81 upright and inverted fluorescent microscopes.
  • Advice on sample preparation, optimization of imaging conditions and image analysis.
  • Typical applications
    • Multi-channel images for most fluorophore combinations
    • Deconvolution of Z-stacks
    • Live-cell imaging with temperature, CO2 and humidity control
    • FRAP
    • FRET
    • Calcium ion imaging
  • We don’t provide:
    • Sample preparation services for widefield microscopy
    • Staff imaging of user-prepared samples

Technical details:

  1. Olympus BX61 Upright Wide Field Microscope
  • Capable of automated time lapse and z-series
  • Imaging modes: Bright Field, Dark Field, DIC,
  • Polarized Light, Epi-fluorescence
  • Manual stage
  • Computer controlled by Perkin Elmer’s Volocity software
  • Hammatsu ORCA RC and QImaging RETIGA 4000R cameras
  • Objective Lenses:
    -4X/0.13 UPlanFLN
    -10X/0.30 UPlanFLN
    -20X/0.50 UPlanFLN
    -40X/1.3 Oil UPlanFLN
    -60X/1.42 Oil PlanApo N
    -100X/1.30 Oil UPlanFLN
  1. Olympus IX81 Inverted Light Microscope
  • Capable of automated time lapse, z-series and multiple x-y position image capture, as well as targeted photobleaching, photoactivation and photostimulation
  • Imaging modes: Bright Field, Phase, DIC, Epi-fluorescence
  • Fully motorized including LEP Bioprecision motorized stage
  • Computer controlled by Perkin Elmer’s Volocity software
  • Capable of automated time lapse, z-series and multiple x-y position image capture
  • Zero Drift Control maintains focus over space and time
  • Bioptechs CO2 and temperature control system for live cell imaging, compatible with 35mm Bioptechs 0400417B dishes.
  • Andor Mosaic photo-bleach system
  • Hammatsu ORCA RC and QImaging RETIGA 4000R cameras
  • Objective Lenses:
    -1.25X/0.04 PlanApoN,
    -2X/0.08 PlanApoN
    -4X/0.13 PhL UPlanFLN
    -10X/0.30 Ph1 UPlanFLN
    -20X/0.45 Ph1 LUCPlanFLN
    -40X/0.60 Ph2 LUCPlanFLN
    -40X/1.30 Oil DIC UPlanFLN,
    -60X/1.42 Oil DIC PlanApo
    -100X/1.3 Oil UPlanFLN
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Olympus BX-61 Olympus IX-81 Fluorescent labeling Immunofluorescence immunohistochemistry Fluorescent insitu hybridization Triple-stain Immunohistochemistry Dual-stain Immunohistochemistry Biology Show 9 more tags Show less

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Live Cell Imaging

Starting at $16.00 per hour

PRICE FOR NON PROFIT: $12/hour
Access to an incubator custom-built incubator microscope, which is capable of time-lapse imaging at multiple locations in phase contrast mode and three fluorescence channels over several days with stable focus, temperature and CO2 concentration.

Technical details:

PRICE FOR NON PROFIT: $12/hour
Access to an incubator custom-built incubator microscope, which is capable of time-lapse imaging at multiple locations in phase contrast mode and three fluorescence channels over several days with stable focus, temperature and CO2 concentration.

Technical details:

  • Imaging modes: Bright Field, Phase, Epi-fluorescence
  • Motorized stage and motorized focus control.
  • Enclosed within an environmental chamber controlling temperature and CO2.
  • Stage compatible with multi-well plates, chambered coverglass slides, chambered slides, and 35 mm dishes.
  • Computer controlled utilizing cellSens software, allowing complicated experimental designs (example: repeated high-speed timelapses at multiple position, repeated multiple times over longer time periods)
  • Hamamatsu Flash 4.0 V2+ sCMOS camera.
  • Filters optimized for the following fluorophores and combinations of fluorophores:
    • GFP/mCherry
    • CFP/YFP/mCherry
    • Cy5
    • Rhodamine
  • Olympus Objective Lenses:
    • 4X/0.13 PhL UPlanFL
    • 10X/0.30 Ph1 UPlanFL
    • 20X/0.45 Ph1 LUCPlanFLN
    • 40X/0.60 Ph2 LUCPlanFLN

For shorter experiments, we can also accommodate live-cell imaging on our inverted widefield and confocal microscopes.

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Olympus IX-70 Olympus CellSens Fluorescent labeling phase-contrast Embryonic stem cells Primary Cells Show 6 more tags Show less

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Image Analysis

Starting at $23.00 per hour

PRICE FOR NON PROFIT: $18/hour

  • Training and access to state-of-the-art image analysis workstation with the following software:
    • Bitplane Imaris 9.2 for 3D analysis
    • Media Cybernetics Autoquant 3.1 for deconvolution (GPU-accelerated)
    • MATLAB
    • FIJI
    • MS Office
  • One-on-one advice on image analysis
  • Typical... Show more »

PRICE FOR NON PROFIT: $18/hour

  • Training and access to state-of-the-art image analysis workstation with the following software:

    • Bitplane Imaris 9.2 for 3D analysis
    • Media Cybernetics Autoquant 3.1 for deconvolution (GPU-accelerated)
    • MATLAB
    • FIJI
    • MS Office
  • One-on-one advice on image analysis

  • Typical applications

    • Deconvolution
    • Colocalization
    • Particle/cell tracking
    • 3D analysis of cells and subcellular structure: measurements of position, shape, intensity, etc.
    • 3D analysis of light-sheet data 100s of GBs in size
  • We don’t provide:

    • Staff analysis of user-provided images
    • Long-term storage of user images

Technical details:

  • CPU: 2 x Intel Xeon E5-2667 V4 3.2GHz (8 core each) with hyperthreading (32 logical cores total)
  • RAM: 512 GB
  • GPU: NIVIDIA PNY Quadro M6000 PCI-E 24GB
  • Storage:
    • 2 x Intel 750 1.2TB PCI-E SSD
    • 3 x Samsung 850 Pro 2TB SATA 6Gb/s 2.5inch SSD in RAID 0
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Bitplane Imaris Media Cybernetics Autoquant

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Bright-Field Microscopy

Starting at $66.60 per hour

PRICE FOR NON PROFIT: $52/hour

  • Training and access to Olympus BX61 and IX81 upright and inverted bright-field microscopes.
  • Advice on sample preparation, optimization of imaging conditions and image analysis.
  • Typical applications
    • Imaging of colorimetric staining (H&E, TUNEL, etc.)
    • High-resolution, low throughput,... Show more »

PRICE FOR NON PROFIT: $52/hour

  • Training and access to Olympus BX61 and IX81 upright and inverted bright-field microscopes.
  • Advice on sample preparation, optimization of imaging conditions and image analysis.
  • Typical applications
    • Imaging of colorimetric staining (H&E, TUNEL, etc.)
    • High-resolution, low throughput, slide-scanning of slides prepared with color stains
    • Medium term (hours) ive-cell imaging in phase-contrast mode with temperature, CO2 and humidity control
  • We don’t provide:
    • Sample preparation services
    • Staff imaging of user-prepared samples

Technical details:

  1. Olympus BX61 Upright Wide Field Microscope
  • Capable of automated time lapse and z-series
  • Imaging modes: Bright Field, Dark Field, DIC,
  • Polarized Light, Epi-fluorescence
  • Manual stage
  • Computer controlled by Perkin Elmer’s Volocity software
  • Hammatsu ORCA RC and QImaging RETIGA 4000R cameras
  • Objective Lenses:
    -4X/0.13 UPlanFLN
    -10X/0.30 UPlanFLN
    -20X/0.50 UPlanFLN
    -40X/1.3 Oil UPlanFLN
    -60X/1.42 Oil PlanApo N
    -100X/1.30 Oil UPlanFLN
  1. Olympus IX81 Inverted Light Microscope
  • Capable of automated time lapse, z-series and multiple x-y position image capture, as well as targeted photobleaching, photoactivation and photostimulation
  • Imaging modes: Bright Field, Phase, DIC, Epi-fluorescence
  • Fully motorized including LEP Bioprecision motorized stage
  • Computer controlled by Perkin Elmer’s Volocity software
  • Capable of automated time lapse, z-series and multiple x-y position image capture
  • Zero Drift Control maintains focus over space and time
  • Bioptechs CO2 and temperature control system for live cell imaging, compatible with 35mm Bioptechs 0400417B dishes.
  • Andor Mosaic photo-bleach system
  • Hammatsu ORCA RC and QImaging RETIGA 4000R cameras
  • Objective Lenses:
    -1.25X/0.04 PlanApoN,
    -2X/0.08 PlanApoN
    -4X/0.13 PhL UPlanFLN
    -10X/0.30 Ph1 UPlanFLN
    -20X/0.45 Ph1 LUCPlanFLN
    -40X/0.60 Ph2 LUCPlanFLN
    -40X/1.30 Oil DIC UPlanFLN,
    -60X/1.42 Oil DIC PlanApo
    -100X/1.3 Oil UPlanFLN
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Olympus BX-61

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Polarized Light Microscopy

Starting at $66.60 per hour

PRICE FOR NON PROFIT: $52/hour

  • Training and access to Olympus BX61 upright bright-field capable of polarization microscopy.

  • Advice on sample preparation, optimization of imaging conditions and image analysis.

  • Typical applications

    • Congo Red staining
    • Birefringence measurements
  • We don’t provide:

PRICE FOR NON PROFIT: $52/hour

  • Training and access to Olympus BX61 upright bright-field capable of polarization microscopy.

  • Advice on sample preparation, optimization of imaging conditions and image analysis.

  • Typical applications

    • Congo Red staining
    • Birefringence measurements
  • We don’t provide:

    • Sample preparation services
    • Staff imaging of user-prepared samples

Technical details:

  1. Olympus BX61 Upright Wide Field Microscope
  • Capable of automated time lapse and z-series
  • Imaging modes: Bright Field, Dark Field, DIC,
  • Polarized Light, Epi-fluorescence
  • Manual stage
  • Computer controlled by Perkin Elmer’s Volocity software
  • Hammatsu ORCA RC and QImaging RETIGA 4000R cameras
  • Objective Lenses:
    -4X/0.13 UPlanFLN
    -10X/0.30 UPlanFLN
    -20X/0.50 UPlanFLN
    -40X/1.3 Oil UPlanFLN
    -60X/1.42 Oil PlanApo N
    -100X/1.30 Oil UPlanFLN
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Dark-Field Microscopy

Starting at $66.60 per hour

PRICE FOR NON PROFIT: $52/hour

  • Training and access to Olympus BX61 upright darkfield-capable microscope.

  • Advice on sample preparation, optimization of imaging conditions and image analysis.

  • We don’t provide:

    • Sample preparation services
    • Staff imaging of user-prepared samples

Technical details:

  1. Olympus BX61... Show more »

PRICE FOR NON PROFIT: $52/hour

  • Training and access to Olympus BX61 upright darkfield-capable microscope.

  • Advice on sample preparation, optimization of imaging conditions and image analysis.

  • We don’t provide:

    • Sample preparation services
    • Staff imaging of user-prepared samples

Technical details:

  1. Olympus BX61 Upright Wide Field Microscope
  • Capable of automated time lapse and z-series
  • Imaging modes: Bright Field, Dark Field, DIC,
  • Polarized Light, Epi-fluorescence
  • Manual stage
  • Computer controlled by Perkin Elmer’s Volocity software
  • Hammatsu ORCA RC and QImaging RETIGA 4000R cameras
  • Objective Lenses:
    -4X/0.13 UPlanFLN
    -10X/0.30 UPlanFLN
    -20X/0.50 UPlanFLN
    -40X/1.3 Oil UPlanFLN
    -60X/1.42 Oil PlanApo N
    -100X/1.30 Oil UPlanFLN
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Olympus BX-61

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Transmitted Light Microscopy

Price on request
Request a quote for more information about this service.

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Project Management

Price on request
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Data Services

Price on request
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Fluorescence-Based Microscopy

Price on request
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Imaging & Spectroscopy

Price on request
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Assay Development

Price on request
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Optical Microscopy

Price on request
Request a quote for more information about this service.

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Microscopy

Price on request
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Electron Microscopy

Price on request
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Computational Modeling

Price on request
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Pablo Ariel

Director and Research Assistant Professor

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