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MicroCT/Histology Core

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Chicago, Illinois, US

About MicroCT/Histology Core

The primary missions of the microCT/histology Core are to provide state-of-the-art three-dimensional ex vivo imaging using microCT, a non-destructive x-ray based imaging modality and to provide histology services. The specimens for microCT must have inherent radio opacity (e.g., bone) or can be rendered radio-opaque (e.g., by the use of contrast agents).

Resources (Instrumentation and Software)

  • Desktop cone-beam microCT scanner Scanco model 40
  • Microfocus x-ray source (5-7 μm spot size) which can operate between 55 and 90 kVp
  • Detector with 2048 by 256 elements
  • Nominal best resolution of 6 μm with a real resolution of 9 μm
  • Nominal best resolution of 6 μm with a real resolution of 9 μm
  • Reconstructed image matrix of either 1024 X 1024 or 2048 X 2048
  • Field of view up to 37 mm and maximum scan length of 70 mm
  • Desktop cone-beam microCT scanner Scanco model 50
  • Microfocus x-ray source (5-30 μm spot size) which can operate between 45 and 90 kVp
  • Detector with 3400 by 1200 elements
  • Nominal best resolution of 0.5 μm with a real resolution of 2 μm
  • Reconstructed image matrix of up to 8192 X 8192
  • Field of view up to 48 mm and maximum scan length of 120 mm
  • Open VMS operating system, with complete imaging software for data acquisition, online/offline reconstruction, 3D visualization and animation, as well as 2D and 3D trabecular bone histomorphometry
  • Database for tracking specimen processing and archiving and the histomorphometric data can be readily downloaded to spreadsheets or statistical packages
  • Faxitron x-ray cabinet (Model MX-20)
  • Saws (Buehler Isomet 5000, Exakt 310CP band saw, Shopmaster band saw), grinder/polisher (Buehler Phoenix 5000), microtome (Leica), embedding equipment for hard and soft tissue histology

Our Services (8)


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microCT

X-ray microtomography
Price on request
  • Ex vivo x-ray micro computed tomographic scanning of specimens
  • 3-D quantification of bone architecture and density
  • 3-D quantification of articular cartilage morphology and proteoglycan content
  • Exporting images (slices) in TIFF or DICOM format
  • Creating 3-D renderings and animations of scanned objects


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Histology and Pathology Services

Price on request
  • Hard tissue (undecalcifed) methylmethacrylate (MMA) processing and embedding
  • Hard tissue (undecalcifed) MMA microtome based thin sections and cut and grind sections, including metal implants
  • Hard tissue (undecalcified) histology
  • Decalcified paraffin processing and embedding
  • Decalcified microtome based thin sections
  • Decalcified histology of bone and soft tissues


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Clinical Research

Price on request
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Clinical and Anatomic Pathology

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Radiography

Price on request
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Imaging & Spectroscopy

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Tomography Services

Computed tomography services
Price on request
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Clinical Laboratory Services

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DS

D. Rick Sumner

Professor and Chair

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