The overall goal of the mCT Core is to facilitate extraordinary transnational research performed by NYU PIs. Our investigators are dedicated to elucidation of new knowledge regarding skeletal acquisition and integrity and to the translation of fundamental advances into clinical practice. Specifically, our mission is to provide high quality and cost effective skeletal phenotyping of animal models of musculoskeletal disease, to optimize image processing, and to train investigators to design and interpret mCT data.
NYU College of Dentistry (NYU Dentistry) core research facilities and equipment offer a broad range of instrumentation and capabilities not often found in academic dental centers. Designed to be shared by investigators both on campus and externally, and to foster collaborative research, these state-of-the-art technologies are playing an important role in the growth of basic, clinical and translational research to improve human health. We invite you to take advantage of NYU Dentistry core scientific resources that are contributing not only to the quality and productivity of research conducted at NYU Dentistry, but also beyond our walls.
Types of Samples Imaged
Imaging Process using Mct
Analysis and Software
All results are reported to the investigator in Excel and summaries can be made if requested. For investigators that would like to quantify on their own, the facility provides access to the software (CTAn) free of charge. However, it is strongly recommended that a training session be scheduled, as the choice of VOIs, drawing of ROIs, and analysis requires some experience in order to provide accurate and consistent data.
Common Morphometric Parameters Calculated by MicroCT
Pilot Scans and Consulting
The core offers free initial imaging consolation and pilot scans if necessary. Here the core will try to optimize the imaging parameters (pixel and matrix) so that the research goals are met while keeping the scan time costs as low as possible. The core has custom designed sample holders to allow for the maximum number of samples to be scanned at one time, while maintaining desired resolution and accuracy. For complex issues related to murine bone collection, storage, and/or study design Dr. Yakar is available on a limited basis. Please contact her directly to set up an appointment.
Data Storage and Backup Policy: Starting July of 2015, the facility is using a Globus server to store/backup ALL raw data up to seven years; however, we are backing up reconstructed data for ONLY six months. Please note, after six months of delivering you the reconstructed data, we will be deleting it permanently. For inquiries regarding raw data before 2015, please contact the operator personally.
Example of a Typical Scanning Protocol
The distal end and mid-shaft of six adult mouse femurs can be scanned at one time in 58 minutes using a pixel size of 9.7 um and a matrix of 2000x1000. If the entire bone needs to be scanned, then an oversized scan with 2 segments is required, thereby increasing the scan time of the six bones to 2 hours. Reconstruction of the six bones takes one hour, regardless of the image size, As a result, imaging and reconstructing of six adult mouse femurs takes 2-3 hours ($150-$225), depending upon whether or not the entire bone needs to be scanned. Based on previous work, analysis of mouse femur takes approximately 45 minutes per bone sample for full Tb and cortical analysis. Construction of cool 2D and/or 3D images takes approximately 15 minutes per bone sample.
MicroCT Core - NYU has not received any reviews.
MicroCT Core - NYU has not received any endorsements.