UNC Microbiome Core

Bioinformatics analysis of 16S rRNA amplicon sequencing data

Illumina sequencing options: MiSeq SE(50) $1,856 MiSeq PE150(300) $2,299 MiSeq PE250(500) $2,572 MiSeq PE300(600) $3,192 HiSeq High output SE50 $2,059 HiSeq High output PE50 $2,810 HiSeq High output SE100 $2,672 HiSeq High output PE100 $4,555 HiSeq 4000 SE50 $2,153 HiSeq 4000 PE50 $2,966 HiSeq 4000 PE100 $4,150 HiSeq 4000 SE100 $2,959 HiSeq 4000 SE150 $3,529 HiSeq 4000 PE150 $4,359 * prices are for UNC, NC State or Federal Funded / all other

The Ion Torrent sequencing platform from Life Technologies employs First PostLight™ sequencing technology (semiconductor technology). The detection of a specific nucleotide on a growing DNA strand occurs inside a well of an Ion Torrent semiconductor chip. The incorporation of the correct base triggers a chemical reaction that releases a hydrogen ion as byproduct, which in turn changes the pH of the solution. The Ion sensor captures voltage change measurements from the direct release of hydrogen ions following DNA polymerization and incorporation events get recorded as digital signals 1 and 0. This technology differs from other sequencing technologies in that no modified nucleotides, no lasers, no optics, no camera, no fluorescence, no enzyme cascade are used.

RNA sequencing (see Illumina sequencing for service options)
Library preparation: $362 RNA extraction: $31