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Microbiome Core Facility

Chapel Hill, North Carolina, US

The human body is the home to approximately 10,000,000,000 bacterial cells that inhabit our gastrointestinal tract, mouth, urogenital tract, and our skin. Most of these bacteria are uncultivable, which meant in the past that researchers were unable to study and characterize them. The development of metagenomic technologies allow us today to examine microbial communities through culture independent techniques, of which the most used are next-generation sequencing methods.

The UNC Microbiome Core Facility was established to provide technical and research support to study community structure and function of host-associated microbial populations.

Microbiome Core Facility has not listed any services.

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DNA Sequencing Data Analysis
Price on request
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Microbiome Studies
Price on request
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NGS Data Analysis
Next generation sequencing data analysis
Starting at $106.00 per hour

Bioinformatics analysis of 16S rRNA amplicon sequencing data

Bioinformatics analysis of 16S rRNA amplicon sequencing data

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16S
Genomic DNA Extraction
Starting at $28.00 per sample
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Illumina Sequencing
Starting at $1,491.00 per lane

Illumina sequencing options:
MiSeq SE5(50) $1,491
MiSeq PE150(300) $2,020
MiSeq PE250(500) $2,315
MiSeq PE300(600) $2,891

HiSeq High output SE50 $1,709
HiSeq High output PE50 $2,891
HiSeq High output SE100 $2,720
HiSeq High output PE100 $3,575
HiSeq High output PE150 $3,668

HiSeq RAPID SE50 $1,861
HiSeq RAPID... Show more »

Illumina sequencing options:
MiSeq SE5(50) $1,491
MiSeq PE150(300) $2,020
MiSeq PE250(500) $2,315
MiSeq PE300(600) $2,891

HiSeq High output SE50 $1,709
HiSeq High output PE50 $2,891
HiSeq High output SE100 $2,720
HiSeq High output PE100 $3,575
HiSeq High output PE150 $3,668

HiSeq RAPID SE50 $1,861
HiSeq RAPID PE50 $3,186
HiSeq RAPID SE100 $2,720
HiSeq RAPID PE100 $3,808
HiSeq RAPID PE150 $4,461
HiSeq RAPID PE250 $5,130

HiSeq 4000 SE50 $2,328
HiSeq 4000 PE50 $2,953
HiSeq 4000 PE100 $3,497
HiSeq 4000 SE100 $2,955
HiSeq 4000 SE150 $3,577
HiSeq 4000 PE150 $3,808
* prices are for UNC, NC State or Federal Funded / all other

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Biology
Price on request
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Ion Torrent Sequencing
Ion Torrent Next Generation Sequencing
Starting at $1,275.00 per chip

The Ion Torrent sequencing platform from Life Technologies employs First PostLight™ sequencing technology (semiconductor technology). The detection of a specific nucleotide on a growing DNA strand occurs inside a well of an Ion Torrent semiconductor chip. The incorporation of the correct base triggers a chemical reaction that... Show more »

The Ion Torrent sequencing platform from Life Technologies employs First PostLight™ sequencing technology (semiconductor technology). The detection of a specific nucleotide on a growing DNA strand occurs inside a well of an Ion Torrent semiconductor chip. The incorporation of the correct base triggers a chemical reaction that releases a hydrogen ion as byproduct, which in turn changes the pH of the solution. The Ion sensor captures voltage change measurements from the direct release of hydrogen ions following DNA polymerization and incorporation events get recorded as digital signals 1 and 0. This technology differs from other sequencing technologies in that no modified nucleotides, no lasers, no optics, no camera, no fluorescence, no enzyme cascade are used.

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RNA Extraction
Starting at $28.00 per sample
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RNA Sequencing
Starting at $364.00 per sample

RNA sequencing (see Illumina sequencing for service options)
Library preparation: $364
RNA extraction: $28

RNA sequencing (see Illumina sequencing for service options)
Library preparation: $364
RNA extraction: $28

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qPCR
Quantitative PCR
Starting at $34.00 per sample
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16S rDNA Clone Library Construction
Starting at $1,155.00 per library

Clone library creation:
In order to confirm bacterial identity, PCR-amplified 16S rRNA gene fragments can be cloned into plasmids and transformed into an appropriate host (usually E. coli). Each transformed E. coli colony will contain only one 16S rDNA PCR-originated fragment from the original sample. A number of colonies are... Show more »

Clone library creation:
In order to confirm bacterial identity, PCR-amplified 16S rRNA gene fragments can be cloned into plasmids and transformed into an appropriate host (usually E. coli). Each transformed E. coli colony will contain only one 16S rDNA PCR-originated fragment from the original sample. A number of colonies are picked randomly and the insert is sequenced. The DNA sequence is then compared to a database (for example RDP II) for identification by DNA homology.

Equipment:
Genetix QPix2 XT Colony Picker System

UNC/NC State/ Covered by federal funds $480
Non-UNC or NC State or not covered by federal funds $528

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Plasmid DNA Preparation
Starting at $28.00 per sample
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Digital PCR
Starting at $34.00 per sample
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Sequencing Data Analysis
Price on request
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DNA Extraction and Purification
Price on request
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Nucleic Acid Analysis
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PCR
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Nucleic Acid Data Analysis
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DNA Libraries
Price on request
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Next Generation Sequencing (NGS)
Price on request
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DNA Sequencing
Price on request
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2018-07-11 16:41:29 -0400

Net Promoter Score of 9 received for Plasmid DNA Preparation.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2018-04-03 11:12:35 -0400

Net Promoter Score of 9 received for qPCR.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2017-10-11 10:40:28 -0400

Net Promoter Score of 10 received for 16S rDNA Clone Library Construction.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 9.
2017-07-05 18:51:59 -0400

Net Promoter Score of 10 received for Plasmid DNA Preparation.

Additional Ratings: satisfaction with deliverable: 4, satisfaction with timeliness: 7.
2017-05-30 12:58:49 -0400

Net Promoter Score of 8 received for Microbiome Studies.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 5.
2017-05-15 18:44:05 -0400

Net Promoter Score of 9 received for Microbiome Studies.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
2016-04-24 23:49:47 -0400

Net Promoter Score of 8 received for 16S Phylogenetic Analysis.

June 23, 2014

Positive review received for Roche 454 Sequencing:

"Nice service."

January 27, 2014

Positive review received for Plasmid DNA Preparation:

"M. Andrea was great to work with. She was extremely accommodating expanding the scope of the project and rushing the deliverable due to a tight deadline on my end. Most importantly the data is clean and samples secure."

January 7, 2013

Positive review received for qPCR:

"Great job! Thanks."

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