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Metabolomics Shared Resource

Washington, District of Columbia, US

The Metabolomics Shared Resource (MSR) here at GUMC has pioneered the development and use of metabolomics for many biomedical applications, and now has added state-of-the-art gas chromatography (GC) mass spectrometry (MS) by the recent award of an instrumentation grant by NIAID (NIH) to Prof. Al Fornace. Drs. Cheema and Fornace co-direct the MSR (in PMSR) and they have primarily relied on liquid chromatography (LC) MS until now. While the current capability provides broad coverage of the metabolome, i.e. small molecules in a biologic sample, it has limitations in being able to efficiently assess many metabolites important in energy metabolism as well as various fatty acids and lipids that are important in many biologic processes. Additionally, the use of electron impact (EI) ionization vs. “soft” LC electrospray ionization reduces cost/analysis time by directly identifying compounds using a well-established NIST... Show more »

The Metabolomics Shared Resource (MSR) here at GUMC has pioneered the development and use of metabolomics for many biomedical applications, and now has added state-of-the-art gas chromatography (GC) mass spectrometry (MS) by the recent award of an instrumentation grant by NIAID (NIH) to Prof. Al Fornace. Drs. Cheema and Fornace co-direct the MSR (in PMSR) and they have primarily relied on liquid chromatography (LC) MS until now. While the current capability provides broad coverage of the metabolome, i.e. small molecules in a biologic sample, it has limitations in being able to efficiently assess many metabolites important in energy metabolism as well as various fatty acids and lipids that are important in many biologic processes. Additionally, the use of electron impact (EI) ionization vs. “soft” LC electrospray ionization reduces cost/analysis time by directly identifying compounds using a well-established NIST fragmentation library. The recently acquired Leco Pegasus HT GCxGC-TOF(time-of-flight)MS now expands the scope of metabolites that can be measured and will provide more comprehensive analyses of diverse biological samples.

This GC-MS is interfaced with the Gerstel autosampler that prevents sample-to-sample carryover. Additionally, the versatile system enables efficient injection, head-space analysis, solid-phase micro-extraction and thermal desorption that contribute to high quality analyses of diverse biological samples.

Recent Publications*

  • Altadill T, Campoy I, Lanau L, Gill K, Rigau M, Gil-Moreno A, Reventos J, Byers S, Colas E and Cheema A. "Enabling Metabolomics Based Biomarker Discovery Studies using Molecular Phenotyping of Exosome-Like Vesicles." PLOS one (2016).
  • Cao L, Liu P, Gill K, Reece EA, Cheema AK, Zhao Z. "2. Identification of novel cell survival regulation in diabetic embryopathy via phospholipidomic profiling." Biochem Biophys Res Commun. 470.3 (2016).
  • Cha, H.-J., and A. J. Fornace, Jr. (2016). P38. p. 1-11. In L. J. Marshall (ed.), Cancer Therapeutic Targets. Springer New York, New York, NY, ISBN: 978-1- 4614-6613- 0.
  • Cheema A., Maier I., Dowdy T, Wang Y, Singh R, Ruegger P, Borneman J, Fornace Jr. A and Schiestl, R. "Chemopreventive Metabolites are Correlated with a Change in Intestinal Microbiota Measured in A-T Mice and Decreased Carcinogenesis." PLOS one e0151190 (2016).
  • Chen, Z., Coy, S. L., Pannkuk, E. L., Laiakis, E. C., Hall, A. B., Fornace, A. J., Jr, and Vouros, P. Rapid and High-Throughput Detection and Quantitation of Radiation Biomarkers in Human and Nonhuman Primates by Differential Mobility Spectrometry-Mass Spectrometry. J Am Soc Mass Spectrom (2016).
  • Datta, K., Suman, S., Kumar, S., and Fornace, A. J. Colorectal Carcinogenesis, Radiation Quality, and the Ubiquitin-Proteasome Pathway. J Cancer 7: 174-183 (2016).
  • Goudarzi, M., Chauthe, S., Strawn, S. J., Weber, W. M., Brenner, D. J., and Fornace, A. J. Quantitative Metabolomic Analysis of Urinary Citrulline and Calcitroic Acid in Mice after Exposure to Various Types of Ionizing Radiation. Int J Mol Sci 17 (2016).
  • Kwon, O. S., Kim, K. T., Lee, E., Kim, M., Choi, S. H., Li, H., Fornace, A. J., Cho, J. H., Lee, Y. S., Lee, J. S., Lee, Y. J., and Cha, H. J. Induction of MiR-21 by Stereotactic Body Radiotherapy Contributes to the Pulmonary Fibrotic Response. PLoS One 11 (2016).
  • Laiakis, E. C., Pannkuk, E. L., Diaz-Rubio, M. E., Wang, Y. W., Mak, T. D., Simbulan-Rosenthal, C. M., Brenner, D. J., and Fornace, A. J., Jr. Implications of genotypic differences in the generation of a urinary metabolomics radiation signature. Mutat Res (2016).
  • Laiakis, E. C., Strawn, S. J., Brenner, D. J., and Fornace, A. J., Jr. Assessment of Saliva as a Potential Biofluid for Biodosimetry: A Pilot Metabolomics Study in Mice. Radiat. Res. Epub ahead of print.
  • Menon SS, Uppal M, Randhawa S, Cheema MS, Aghdam N, Usala RL, Ghosh SP, Cheema AK, Dritschilo A. "1. Radiation Metabolomics: Current Status and Future Directions." Front Oncol 6.20 (2016).
  • Pannkuk, E. L., Laiakis, E. C., Authier, S., Wong, K., and Fornace, A. J., Jr. Targeted metabolomics of nonhuman primate serum after exposure to ionizing radiation: potential tools for high-throughput biodosimetry. RSC Adv. 6: 51192-51202, 2016.
  • Pannkuk, E. L., Laiakis, E. C., Mak, T. D., Astarita, G., Authier, S., Wong, K., and Fornace, A. J., Jr. A lipidomic and metabolomic serum signature from nonhuman primates exposed to ionizing radiation. Metabolomics in press (2016).
  • Yauk, CL, Buick, JK, Williams, A, Swartz, CD, Recio, L, Li, HH, Fornace, AJ Jr., Thomson, EM, Aubrecht, J.Application of the TGx-28.65 transcriptomic biomarker to classify genotoxic and non-genotoxic chemicals in human TK6 cells in the presence of rat liver S9. Environ Mol Mutagen (2016).
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GC-MS
Gas Chromatography Coupled Mass Spectrometry
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GCMS is useful analytical technique that combines mass spectrometry and gas chromatography for accurate measurement of volatile organic and inorganic compounds. Manufacturer supplied software with built-in spectral libraries makes GCMS makes for easy identification of compounds. Our Metabolics Shared Resources core has recently... Show more »

GCMS is useful analytical technique that combines mass spectrometry and gas chromatography for accurate measurement of volatile organic and inorganic compounds. Manufacturer supplied software with built-in spectral libraries makes GCMS makes for easy identification of compounds. Our Metabolics Shared Resources core has recently acquired a new Leco Pegasus HT GC-TOFMS, which will expand the range of metabolites that we will be able assess.

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Leco Pegasus GCTOF MS
Mass Spectrometry
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The SYNAPT G2-Si High Definition Mass Spectrometer provides the most complete characterization of complex mixtures and molecules with unique levels of MS performance, industry leading informatics and unparalleled platform versatility. With high-efficiency T-Wave ion mobility, this can be employed for an additional dimension of separation, based on molecular size and shape.

The SYNAPT G2-Si High Definition Mass Spectrometer provides the most complete characterization of complex mixtures and molecules with unique levels of MS performance, industry leading informatics and unparalleled platform versatility. With high-efficiency T-Wave ion mobility, this can be employed for an additional dimension of separation, based on molecular size and shape.

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Synapt G2
LC-MS
Liquid Chromatography-Coupled Mass Spectrometry
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All the mass spectrometry instruments are coupled to an Ultra Performance Liquid Chromatography (UPLC). The Premier, Xevo G2, and SYNAPT G2-Si are utilized for profiling and tandem mass spectrometry, while the Xevo TQ-S is utilized for sensitive and absolute quantification.

All the mass spectrometry instruments are coupled to an Ultra Performance Liquid Chromatography (UPLC). The Premier, Xevo G2, and SYNAPT G2-Si are utilized for profiling and tandem mass spectrometry, while the Xevo TQ-S is utilized for sensitive and absolute quantification.

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Waters Xevo G2 QTOF
Synapt G2
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Liquid Chromatography Coupled Mass Spectrometry Methods
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Metabolomics
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