The Metabolomics Shared Resource (MSR) here at GUMC has pioneered the development and use of metabolomics for many biomedical applications, and now has added state-of-the-art gas chromatography (GC) mass spectrometry (MS) by the recent award of an instrumentation grant by NIAID (NIH) to Prof. Al Fornace. Drs. Cheema and Fornace co-direct the MSR (in PMSR) and they have primarily relied on liquid chromatography (LC) MS until now. While the current capability provides broad coverage of the metabolome, i.e. small molecules in a biologic sample, it has limitations in being able to efficiently assess many metabolites important in energy metabolism as well as various fatty acids and lipids that are important in many biologic processes. Additionally, the use of electron impact (EI) ionization vs. “soft” LC electrospray ionization reduces cost/analysis time by directly identifying compounds using a well-established NIST fragmentation library. The recently acquired Leco Pegasus HT GCxGC-TOF(time-of-flight)MS now expands the scope of metabolites that can be measured and will provide more comprehensive analyses of diverse biological samples.
This GC-MS is interfaced with the Gerstel autosampler that prevents sample-to-sample carryover. Additionally, the versatile system enables efficient injection, head-space analysis, solid-phase micro-extraction and thermal desorption that contribute to high quality analyses of diverse biological samples.
GCMS is useful analytical technique that combines mass spectrometry and gas chromatography for accurate measurement of volatile organic and inorganic compounds. Manufacturer supplied software with built-in spectral libraries makes GCMS makes for easy identification of compounds. Our Metabolics Shared Resources core has recently acquired a new Leco Pegasus HT GC-TOFMS, which will expand the range of metabolites that we will be able assess.
The SYNAPT G2-Si High Definition Mass Spectrometer provides the most complete characterization of complex mixtures and molecules with unique levels of MS performance, industry leading informatics and unparalleled platform versatility. With high-efficiency T-Wave ion mobility, this can be employed for an additional dimension of separation, based on molecular size and shape.
All the mass spectrometry instruments are coupled to an Ultra Performance Liquid Chromatography (UPLC). The Premier, Xevo G2, and SYNAPT G2-Si are utilized for profiling and tandem mass spectrometry, while the Xevo TQ-S is utilized for sensitive and absolute quantification.
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