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MD Biosciences Preclinical Laboratory Services

Oakdale, Minnesota, US

MD Biosciences Preclinical Laboratory Services is a niche preclinical research group working with biopharmacuetical and medical device companies. Our core therapeutic areas are oncology, inflammation, CNS, pain and cardiovascular with an emphasis on the interplay between these systems.

We place a great deal of importance on the scientific rationale of each individual study to ensure that it meets the goals of the R&D program. We are willing to invest in custom study design, development and validation with our sponsors to achieve clinically relevant data at the preclinical stages. We feel that this will help reduce the failure rate in clincial stages and the burden upon the industry.

We employ a highly talented group of scientists consisting of PhD, MSc and Bachelor degrees. Each scientist is specialized and highly skilled in their area of expertise. Our group has all been trained in various techniques... Show more »

MD Biosciences Preclinical Laboratory Services is a niche preclinical research group working with biopharmacuetical and medical device companies. Our core therapeutic areas are oncology, inflammation, CNS, pain and cardiovascular with an emphasis on the interplay between these systems.

We place a great deal of importance on the scientific rationale of each individual study to ensure that it meets the goals of the R&D program. We are willing to invest in custom study design, development and validation with our sponsors to achieve clinically relevant data at the preclinical stages. We feel that this will help reduce the failure rate in clincial stages and the burden upon the industry.

We employ a highly talented group of scientists consisting of PhD, MSc and Bachelor degrees. Each scientist is specialized and highly skilled in their area of expertise. Our group has all been trained in various techniques including microsurgical procedures, injection methods, model and method development as well as imaging and histological techniques.

Selected Publications

  • Castel, D., Willentz, E., Doron, O., Brenner, O. and Meilin, S. (2014), Characterization of a porcine model of post-operative pain. European Journal of Pain, 18: 496–505. doi: 10.1002/j.1532-2149.2013.00399.x
  • Meilin, S., Machicao, F., & Elmlinger, M. (2014). Treatment with Actovegin improves spatial learning and memory in rats following transient forebrain ischaemia. Journal of Cellular and Molecular Medicine (8), 1623–1630. doi:10.1111/jcmm.12297
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Neurochemistry Animal Models
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Drug Development
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Hair Growth Animal Models
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Flow Cytometry Imaging
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Flow Cytometry Experimental Design
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Flow Cytometry Data acquisition
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Flow Cytometry Data Analysis
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FACS
Fluorescence Activated Cell Sorting
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Cell Cycle Analysis
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Custom CNS Animal Models
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Neurodegenerative diseases like Parkinson's as well as neuro-inflammatory diseases such as multiple sclerosis (MS), with additional capabilities in stroke and cerebral ischemia. Offering customizable study options upon request.

Neurodegenerative diseases like Parkinson's as well as neuro-inflammatory diseases such as multiple sclerosis (MS), with additional capabilities in stroke and cerebral ischemia. Offering customizable study options upon request.

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Experimental Autoimmune Encephalomyelitis (EAE) induced- neuropathy
6-OHDA- induced parkinson model
MPTP-induced Parkinson's disease model
PLP-induced Experimental Autoimmune Encephalomyelitis (EAE)
Spinal Cord Homogenate-induced EAE
Chronic Constriction Injury Animal Models
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the left sciatic nerve is ligated causing inflammation and swelling, and ultimately peripheral pain.

the left sciatic nerve is ligated causing inflammation and swelling, and ultimately peripheral pain.

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sprague dawley rat
c57/B16 mouse
Cell-Based Screening Methods
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Cell Signaling Assays
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Cell Death Assays
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Colony Formation Assays
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Cell Adhesion Assays
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BrdU Assay
Bromodeoxyuridine Assay
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Cell Migration Assays
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Electroporation
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Cell Co-Cultivation
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Extracellular Vesicle Analysis
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Electrophysiology
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Dantec KEYPOINT Focus tool utilized to measure electrophysiological action potentials. Capabilities in the assessment of muscle, motor and sensory action potential in rodent models of peripheral nerve injury to measure nerve regeneration, degeneration and protection in response to various treatments. Can apply to non-rodent species and pig models.

Dantec KEYPOINT Focus tool utilized to measure electrophysiological action potentials. Capabilities in the assessment of muscle, motor and sensory action potential in rodent models of peripheral nerve injury to measure nerve regeneration, degeneration and protection in response to various treatments. Can apply to non-rodent species and pig models.

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Custom Animal Models
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Forced Swim Test
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A behavioral test used on rats to assess responses to threat of drowning

A behavioral test used on rats to assess responses to threat of drowning

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Exploratory Animal Studies
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Elevated Plus Maze Animal Anxiety Test
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A test measuring anxiety in laboratory animals that usually uses rodents as a screening test

A test measuring anxiety in laboratory animals that usually uses rodents as a screening test

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Cylinder Test
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Asymmetry analysis assesses behavioral deficits in rats after stroke or brain injury.

Asymmetry analysis assesses behavioral deficits in rats after stroke or brain injury.

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Dose Range Finding (DRF) Studies
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Caco-2 Cell Permeability Assay
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Quantitative Western Blot Analysis
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Protein Sample Preparation
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Primer Design
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Pan-Genome Sequencing
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PCR Purification
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PCR
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Porcine Models
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Large animal models offer high translatability from preclinical research to clinical trials. We offer pig models in neuropathic pain, post-operative pain, wound healing and other dermatology studies and pig nerve block.

Large animal models offer high translatability from preclinical research to clinical trials. We offer pig models in neuropathic pain, post-operative pain, wound healing and other dermatology studies and pig nerve block.

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Patient Derived Cancer Cell Models
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Pulmonary Inflammation Animal Models
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MD Biosciences offers models to study the inflammatory components involved in lung inflammation and asthma including OVA-induced asthma, HDM-induced asthma, LPS-acute lung injury, and bleomycin-induced fibrosis.

MD Biosciences offers models to study the inflammatory components involved in lung inflammation and asthma including OVA-induced asthma, HDM-induced asthma, LPS-acute lung injury, and bleomycin-induced fibrosis.

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Mouse LPS
Rat LPS
OVA Asthma
Bleomycin-induced Scleroderma/Systemic Sclerosis
Peritonitis Animal Models
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Polymorphism Screening
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Polymorphism

Polymorphism

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Prothrombin Time Test
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Partial Thromboplastin Time Test
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Prolactin Testing
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Progesterone Testing
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Pregnancy Testing
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Parathyroid Hormone Testing
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Periodic Acid-Schiff (PAS) Staining
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Preclinical Histology
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Primary Cell Isolation
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Primary Biopsy Culture
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Primary Cell Lines
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Porcine Dermis
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Plastic Methyl Methacrylate Embedding
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Paraffin Sectioning
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Paraffin Embedding
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PBMC Isolation
Peripheral Blood Mononuclear Cell Isolation
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Peptide Scanning
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Plasma Bioanalysis
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Peroxidase Assays
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Preclinical Efficacy Studies
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in vitro, in vivo and ex vivo modeling services for efficacy determinations, PK/PD assessments, mechanism of action studies, compound screening, biomarker analysis and more!

in vitro, in vivo and ex vivo modeling services for efficacy determinations, PK/PD assessments, mechanism of action studies, compound screening, biomarker analysis and more!

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Brown Rat (Rattus norvegicus)
Porcine
murine
Cancer
Diabetes Type 1
Diabetes Type 2
Rheumatoid Arthritis
Fibrosis
Obesity
Sepsis
Diabetes
neurodegenerative diseases
neuropathy
multiple sclerosis
Experimental Autoimmune Encephalomyelitis (EAE)
colitis
dermatitis
psoriasis
inflammatory Bowel Disease
Wound Care
inflammation
Cystic Fibrosis
Asthma
Parkinsons Disease
Pain
Metabolic disorders
pulmonary fibrosis
Acute Kidney Injury
CNS/neurology
Collagen Induced Arthritis
Collagen Antibody-Induced Arthritis
Adjuvant-Induced Arthritis
Renca Renal Cancer
Preclinical Study Design
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In vivo Pharmacology Studies
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MD Biosciences features a wide range of validated in vivo models of human disease to support preclinical drug efficacy tests, and we provide customizable disease-specific models in order to fit each study's needs.

MD Biosciences features a wide range of validated in vivo models of human disease to support preclinical drug efficacy tests, and we provide customizable disease-specific models in order to fit each study's needs.

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ADME/DMPK Studies
Drug Metabolism and Pharmacokinetics
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Animal Model in vivo Analyses
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Variety of preclinical animal models for pharmacology, efficacy and mechanism of action studies in addition to biomarker analysis

Variety of preclinical animal models for pharmacology, efficacy and mechanism of action studies in addition to biomarker analysis

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Mouse
Pig
Rat
Collagen-induced arthritis
DSS- induced colitis
Diet-induced obesity
transgenic rat
transgenic mouse
DNCB Induced Contact Dermatitis
tail flick
visceral
Capsaicin
bilateral AKI
HDM
Rat IMQ
Mouse IMQ
Collagen antibody induced rheumatoid arthritis
pig POP model
pig neuropathic pain
Il-23-induced Psoriasis
Fluorescein Isothiocynate Dermatitis
Oxazalone Delayed Type Hypertrophy
Passive Cutaneous Anaphylaxis
OVA Asthma
Allergic Asthma
Lipoplysaccharide Lung
Fibrosis-Belomycin
Collagen-induced rheumatoid arthritis
Adjuvant-Induced rheumatoid arthritis
Myelin Oligodentrocyte Glycoprotein
Proteolipid Protein
Myelin Basic Protein
Acute 1-Methyl-4Phenyl-1,2,3,6-Tetrahydropyridine (MPTP)
Middle Cerebral Artery Occlusion (MCAo)
Four Vessel Occlusion (4VO)
Non-Obese Diabetic (NOD)
Steptozotocin (STZ)
Dextran Sodium Sulfate (DSS)
2,4,6-trinitrobenzenesulfonic acid (TNBS)
Unilateral Kidney Injury (AKI)
Sepsis Acute Kidney Injury (AKI)
Chronic Constrictive Injury (CCI)
Spinal Nerve Ligation (SNL)
Cuff
Crush
Streptozotocin (STZ)
Complete Fruend Adjuvant (CFA)
Carageenan
Monosodium Iodoacetate (MIA)
Poly Arthritic
Mono Arthritic
Brennen
Nerve Block
Incision
Excision
Burn
Rat (Permanent)
SEB-induced T-cell Anergy
House dust mite induced asthma
Bleomycin-induced Scleroderma/Systemic Sclerosis
NASH
Non-alcoholic steatohepatitis
Renal Fibrosis
Fibrosis
Drug Potency and Efficacy Testing
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High Throughput Screening (HTS)
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Drug Distribution Studies
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Biomarker Analysis
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Variety of methods across all major platforms for biomarker identification, development, analysis, qualification, validation and downstream application

Variety of methods across all major platforms for biomarker identification, development, analysis, qualification, validation and downstream application

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Human
Mouse
Pig
Rat
large animals
Flow cytometry
Immunoassays
FFPE tissue
Mammalian tissue
ELISA
Immunofluorescence
qPCR
western blot
immunohistochemistry
IHC
Frozen Tissue
Triple-stain Immunohistochemistry
Dual-stain Immunohistochemistry
Diseased human tissue
Normal Human tissue
Cell-based assays
tissue section
Tissue Block
Immunogenicity Studies
Immunophenotyping
Non-human primates
qPCR Assay
ELISA-based assay
Western blotting
Epigenetics
Fresh Tissue
Fresh Tumors
whole tissue
multiplex Western blot
western blot normalization
western blot optimization
quantitative western blot
Canine
Feline
ELISA Protein Analysis
Real time qPCR
Clinical chemistry
Biomarker Discovery
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Our capabilities include support for projects at every stage in the development pipeline, from the initial biomarker identification, qualification and validation through to downstream clinical applications. Some services include identification of druggable targets, characterization of indicators of disease severity or stratification of patient populations.

Our capabilities include support for projects at every stage in the development pipeline, from the initial biomarker identification, qualification and validation through to downstream clinical applications. Some services include identification of druggable targets, characterization of indicators of disease severity or stratification of patient populations.

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Flow cytometry
assay development
Western blotting
ELISA Protein Analysis
Multiplexed Protein Analysis
Serology
Real time qPCR
Multiplexed mRNA analysis
Multiplexed miRNA analysis
Tissue Extraction
Urinalysis
Hematology
Clinical chemistry
Animal Behavior Studies
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Animal Model Housing and Maintenance
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Demyelination Animal Models
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experimental autoimmune encephalomyelitis (EAE) and MS, EAE has been used as a model for the study of human demyelinating disease.

experimental autoimmune encephalomyelitis (EAE) and MS, EAE has been used as a model for the study of human demyelinating disease.

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Autoimmune Disease Animal Models
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proliferation
Cellular Immunity
Non-specific Immunity
Humoral Immunity
Allergy/Hypersensitivity
Th17 activation
T cell inhibition
Cytokine Release
T cell stimulation
Macrophage (Inhibition)
Macrophage (stimulation)
In vivo Drug Efficacy Testing
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Combination Therapy Efficacy Testing in Mice
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Troponin Assay
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ELISA
Enzyme-linked immunosorbent assay
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plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones

plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones

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Acute Inflammatory Pain Animal Models
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offers commonly used models of acute inflammatory pain such as CFA and carrageenan - that can be extended - to evaluate a more chronic inflammatory pain state. We can help you study the release of Cytokines and their role in the initiation and maintenance of the inflammatory response along with prostaglandin

offers commonly used models of acute inflammatory pain such as CFA and carrageenan - that can be extended - to evaluate a more chronic inflammatory pain state. We can help you study the release of Cytokines and their role in the initiation and maintenance of the inflammatory response along with prostaglandin

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CFA
Carrageenan
Visceral Pain Animal Models
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Used in nociceptive pain models

Used in nociceptive pain models

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IHC
Biomarker analysis
PK
von frey
Motor Function
Osteoarthritis Animal Models
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Monosodium iodoacetate (MIA) induced osteoarthritis in rats. Cartilage damage and degradation leads to a chronic neuronal damage with neuropathic characteristics.

Monosodium iodoacetate (MIA) induced osteoarthritis in rats. Cartilage damage and degradation leads to a chronic neuronal damage with neuropathic characteristics.

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histology
Open field
Rotorod
joint pain
weight bearing
Thermal Hyperalgesia
mechanical Allodynia
paw edema
joint compression
Psoriasis Animal Models
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Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, and passive cutaneous anaphylaxis are our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry.

Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, and passive cutaneous anaphylaxis are our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry.

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Imiquimod-induced psoriasis
Atopic Dermatitis Animal Models
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FITC Induced Contact Dermatitis
DNCB Induced Contact Dermatitis
Custom Pain Animal Models
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Animal Nociception Tests
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MD Biosciences offers a wide range of pre-clinical pain models for nociception that can be customized according to sponsors criteria. Validated models include tail flick, visceral and capsaicin.

MD Biosciences offers a wide range of pre-clinical pain models for nociception that can be customized according to sponsors criteria. Validated models include tail flick, visceral and capsaicin.

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tail flick
visceral
Capsaicin
Ischemia Animal Models
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Focal ischemia (MCAo) models are commonly used to mimic the human stroke situation, however the global ischemia model (4VO) would be highly relevant in clinical conditions such as cardiac arrest.

Focal ischemia (MCAo) models are commonly used to mimic the human stroke situation, however the global ischemia model (4VO) would be highly relevant in clinical conditions such as cardiac arrest.

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MCAo
4Vo
In vitro Diabetes Models
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The non-obese diabetic (NOD) mouse model develops spontaneous autoimmune diabetes, which shares similarities with type 1 diabetes. The STZ-induced diabetic model involves an autoimmune process that results in the destruction of Langerhans islets.

The non-obese diabetic (NOD) mouse model develops spontaneous autoimmune diabetes, which shares similarities with type 1 diabetes. The STZ-induced diabetic model involves an autoimmune process that results in the destruction of Langerhans islets.

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NOD
STZ
unilateral AKI
bilateral AKI
DIO rat
DIO mouse
Inflammation Assays
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Anaphylaxis Animal Models
PCA Animal Model
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Passive Cutaneous Anaphylaxis (PCA) is an immediate dermal response to an allergan-IgE interaction. It is based on a passively transferred anaphylactic reaction from antiserum. Assessments include dry extravasation (mm), absorbancy, PK blood analysis and biomarker analysis.

Passive Cutaneous Anaphylaxis (PCA) is an immediate dermal response to an allergan-IgE interaction. It is based on a passively transferred anaphylactic reaction from antiserum. Assessments include dry extravasation (mm), absorbancy, PK blood analysis and biomarker analysis.

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Delayed-Type Hypersensitivity (DTH) Animal Models
Delayed Type Hypersensitivity Animal Models
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Delayed Type Hypersensitivity (DTH) model evaluates the cell-mediated immune response to repeated exposure of skin to oxazolone. This results in a DTH reaction involving CD4+, CD8+ T cells and macrophages, resulting in local skin inflammation characterized by an increased thickness of the skin.

Delayed Type Hypersensitivity (DTH) model evaluates the cell-mediated immune response to repeated exposure of skin to oxazolone. This results in a DTH reaction involving CD4+, CD8+ T cells and macrophages, resulting in local skin inflammation characterized by an increased thickness of the skin.

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Contact Dermatitis Animal Models
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FITC & DNCB-induced Contact Dermatitis Models. These models share many of the features of human contact dermatitis, both sensitization and challenge occurs epicutaneously.

FITC & DNCB-induced Contact Dermatitis Models. These models share many of the features of human contact dermatitis, both sensitization and challenge occurs epicutaneously.

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FITC Induced Contact Dermatitis
DNCB Induced Contact Dermatitis
In vivo Psoriasis Assay
Price on request

IMQ-Induced Psoriasis Assay

This rapid model of psoriasis-like inflammation involves the IL-17/IL-23 axis. Imiquimod is a potent TLR7/8 ligand and potent immune modulator. Application of imiquimod on the back and ear of mice induces a psoriasis-like inflammation.

IMQ-Induced Psoriasis Assay

This rapid model of psoriasis-like inflammation involves the IL-17/IL-23 axis. Imiquimod is a potent TLR7/8 ligand and potent immune modulator. Application of imiquimod on the back and ear of mice induces a psoriasis-like inflammation.

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Rat IMQ
Mouse IMQ
Lung Inflammation Animal Models
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Following induction by the intranasal administration of LPS, leukocytes (mainly neutrophils) are recruited to the lung. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.

Following induction by the intranasal administration of LPS, leukocytes (mainly neutrophils) are recruited to the lung. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.

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LPS
LPS
Colitis Animal Models
Price on request

Acute DSS-induced colitis in mice and rats
Chronic DSS-induced colitis in mice
TNBS-induced colitis in mice and rats

Acute DSS-induced colitis in mice and rats
Chronic DSS-induced colitis in mice
TNBS-induced colitis in mice and rats

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Mouse
Rat
Rheumatoid Arthritis Animal Models
Price on request

Collagen induced arthritis (CIA) and adjuvant induced arthritis (AIA) are two commonly used models for this purpose. At MD Biosciences we offer our clients the novel and rapid collagen antibody-induced arthritis (CAIA) model as well.

Collagen induced arthritis (CIA) and adjuvant induced arthritis (AIA) are two commonly used models for this purpose. At MD Biosciences we offer our clients the novel and rapid collagen antibody-induced arthritis (CAIA) model as well.

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Collagen-induced arthritis
Collagen antibody induced rheumatoid arthritis
Global Ischemia 4VO Animal Model
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The Global Ischemia model involves permanent occlusion of vertebral arteries and transient occlusion of carotid arteries. The 4VO model can be used to evaluate not only lack of blood flow to brain as found in cardiac arrest and coronary bypass surgery, but also neurodegeneration, dementia and oxygen stress.

The Global Ischemia model involves permanent occlusion of vertebral arteries and transient occlusion of carotid arteries. The 4VO model can be used to evaluate not only lack of blood flow to brain as found in cardiac arrest and coronary bypass surgery, but also neurodegeneration, dementia and oxygen stress.

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Rat permanent 4VO
Rat transient 4VO
Sepsis Animal Models
Price on request

LPS is used to induce sepsis in mice, setting off an immune response activating immune cells that excrete cytokines.

LPS is used to induce sepsis in mice, setting off an immune response activating immune cells that excrete cytokines.

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Sepsis induced by LPS
Mouse LPS
Focal Cerebral Ischemia Animal Models
Price on request

The focal cerebral ischemic model is commonly used to assess ischemic injury in the brain, evaluate stroke or neurodegeneration as a result of oxygen stress.

The focal cerebral ischemic model is commonly used to assess ischemic injury in the brain, evaluate stroke or neurodegeneration as a result of oxygen stress.

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Mouse transient MCAo
Rat transient MCAo
Rat permanent MACo
Postoperative Pain Animal Models
Price on request

Validated POP and Brennan models using pigs and mice. Pigs demonstrate notable resemblance to human anatomy & physiology. This includes analogous dermal epidermal thickness, dermal papillary bodies and abundant subdermal adipose tissue to humans. Functionally, pig and human are similar in epidermal turnover time, type of keratinous proteins and lipid composition of the stratum corneum.

Validated POP and Brennan models using pigs and mice. Pigs demonstrate notable resemblance to human anatomy & physiology. This includes analogous dermal epidermal thickness, dermal papillary bodies and abundant subdermal adipose tissue to humans. Functionally, pig and human are similar in epidermal turnover time, type of keratinous proteins and lipid composition of the stratum corneum.

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Rat Brennan model
pig POP model
Asthma Animal Models
Price on request

The murine OVA-induced asthma model is a widely used model that results in the characteristic features of asthma allowing the study and assessment of novel treatments.
he House Dust Mite (HDM) murine model of allergic asthma is used to assess therapies with potential indications in airway inflammation and/or pulmonary... Show more »

The murine OVA-induced asthma model is a widely used model that results in the characteristic features of asthma allowing the study and assessment of novel treatments.
he House Dust Mite (HDM) murine model of allergic asthma is used to assess therapies with potential indications in airway inflammation and/or pulmonary inflammation driven primarily by repeated exposure to allergens. Model includes Th2- associated cytokine response, eosinophilic infiltration into the lungs, airway hyper responsiveness (AHR) and epithelial tissue remodeling.

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OVA
HDM
LPS
Nociceptive Pain Animal Models
Price on request

MD Biosciences offers a wide range of pre-clinical pain models for nociception that can be customized according to sponsors criteria.

MD Biosciences offers a wide range of pre-clinical pain models for nociception that can be customized according to sponsors criteria.

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tail flick
visceral
Capsaicin
Nerve Block Animal Models
Price on request

The rapid preclinical model of sciatic nerve block enables researchers to directly access and dose to the sciatic nerve and allows the screening of new nerve blocking agents or drugs designed to reverse the local analgesia. The capability can be applied to other models such as the SNL and CCI models.

The rapid preclinical model of sciatic nerve block enables researchers to directly access and dose to the sciatic nerve and allows the screening of new nerve blocking agents or drugs designed to reverse the local analgesia. The capability can be applied to other models such as the SNL and CCI models.

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rat model
pig model
Neuropathic Pain Animal Models
Price on request

Models in both the rodent as well as the pig bringing much needed translational value from preclinical studies to the human situation.

Models in both the rodent as well as the pig bringing much needed translational value from preclinical studies to the human situation.

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nerve injury
chemotherapy
STZ diabetic
pig neuropathic pain
Myocardial Ischemia Reperfusion Injury Animal Models
Price on request
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ligation induced acute cardiac infarction
Coil-induced acute cardiac infarction
multi-focal myocardial infarction
balloon-induced acute cardiac reperfusion
Induction of vascular disease
Chemotherapy-Induced Peripheral Neuropathy Animal Models
Price on request

We conduct preclinical neuropathy models induced with Vincristine or Taxol to mimic the peripheral neuropathy that often results from chemotherapy treatments.

We conduct preclinical neuropathy models induced with Vincristine or Taxol to mimic the peripheral neuropathy that often results from chemotherapy treatments.

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Rat taxol
Rat vincristine
Acute Kidney Failure Models
Price on request

MD Biosciences has validated ischemia-induced renal injury models (unilateral and bilateral) and has familiarity with a variety of other methods should Sponsors have interest in performing validation work on a cost-share basis.

MD Biosciences has validated ischemia-induced renal injury models (unilateral and bilateral) and has familiarity with a variety of other methods should Sponsors have interest in performing validation work on a cost-share basis.

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LPS
Mouse LPS
Rat LPS
Sepsis Acute Kidney Injury (AKI)
Unilateral Kidney Injury
Bilateral Acute Kidney Injury
Parkinsons Disease Animal Models
Price on request

We offer the classic 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)- and 6-hydroxydopamine (6-OHDA)-induced neurotoxin models and genetic models of the disease.

We offer the classic 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)- and 6-hydroxydopamine (6-OHDA)-induced neurotoxin models and genetic models of the disease.

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acute MPTP
60HDA
Spinal Cord Injury Models
Price on request

MDB offers a validated neurite outgrowth assay provides an in vitro method to examine the potential effects of exogenous compounds on neuronal activity and development, providing valuable information on the potential of therapeutic compounds to either enhance or inhibit neuritogenesis.

MDB offers a validated neurite outgrowth assay provides an in vitro method to examine the potential effects of exogenous compounds on neuronal activity and development, providing valuable information on the potential of therapeutic compounds to either enhance or inhibit neuritogenesis.

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neurite outgrowth
In vivo Phenotypic Screening
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LPS-Induced Inflammation Animal Models
Lipopolysaccharide-Induced Inflammation Animal Models
Price on request

The LPS lung inflammation model is an acute lung inflammation model where researchers can observe the pulmonary response following exposure to bacteria. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.

The LPS lung inflammation model is an acute lung inflammation model where researchers can observe the pulmonary response following exposure to bacteria. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.

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F4/80 microglia cells
GFAP
Wound Healing Animal Models
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MD Biosciences offers various models of wound healing in both rodents and pigs. While rodent models provide data for earlier research stages, pig models are ideally suited for wound healing efficacy studies.

MD Biosciences offers various models of wound healing in both rodents and pigs. While rodent models provide data for earlier research stages, pig models are ideally suited for wound healing efficacy studies.

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mouse incisional wound
rat incisional wound
rodent burn wound
pig incisional wound
pig excisional wound
pig burn wound
Custom Cell-Based Assays
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Custom Assays
PBMC
human primary cells
immortalized cell lines
murine
rat
ex vivo primary cells
Obesity Animal Models
Price on request

We conduct metabolic and inflammation research, with diet-induced rat and mouse models, and including the following assessments: feed intake, body weight, insulin levels, glucose tolerance, blood pressure, feed preparing, and biomarkers.

We conduct metabolic and inflammation research, with diet-induced rat and mouse models, and including the following assessments: feed intake, body weight, insulin levels, glucose tolerance, blood pressure, feed preparing, and biomarkers.

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Diet-induced obesity
DIO rat
DIO mouse
Metabolic Animal Models
Price on request

Metabolic disease services include models for diabetes, renal, obesity and inflammatory bowel disease. Multiple induction methods may be available for each disease. Diabetes models may be induced with streptozotocin, the use of db/db or NOD mice. Inflammatory Bowel Disease (IBD) may be induced with TNBS or DSS.

Metabolic disease services include models for diabetes, renal, obesity and inflammatory bowel disease. Multiple induction methods may be available for each disease. Diabetes models may be induced with streptozotocin, the use of db/db or NOD mice. Inflammatory Bowel Disease (IBD) may be induced with TNBS or DSS.

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obesity
diabetes
acute kidney injury
IBD
Clinical Laboratory Services
Price on request

MD Biosciences is proud to offer comprehensive diagnostic services for sample analysis and assay development performed to CLIA and GLP regulatory compliance. We provide a wide range of services for specialized diagnostic testing, clinical trial testing, GLP and GMP assessments of compounds/biologics and more! Our goal is to... Show more »

MD Biosciences is proud to offer comprehensive diagnostic services for sample analysis and assay development performed to CLIA and GLP regulatory compliance. We provide a wide range of services for specialized diagnostic testing, clinical trial testing, GLP and GMP assessments of compounds/biologics and more! Our goal is to facilitate the advancement of diagnostic capabilities and improve analytical methods through application of rigorous biomarker-based strategies.

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Flow cytometry
assay development
real-time PCR
Western blotting
ELISA Protein Analysis
Multiplexed Protein Analysis
Serology
Multiplexed mRNA analysis
Multiplexed miRNA analysis
Tissue Extraction
Urinalysis
Hematology
Clinical chemistry
Cardiovascular Animal Models
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Animal Learning, Memory, and Behavior Tests
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Immunology Animal Models
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Cellular Immunity
Non-specific Immunity
Humoral Immunity
Allergy/Hypersensitivity
Safety Pharmacology & Efficacy Testing
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Drug Discovery & Development
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Cells and Tissues
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Infectious Disease Animal Models
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RNA
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Bioanalysis
Price on request

Bioanaylsis for all sample types across all major methodologies.

Bioanaylsis for all sample types across all major methodologies.

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Protein Quantification
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Nephrology Animal Models
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Immune Cell Assays
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Protein Services
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Functional Human Tissue Assays
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Animal Cognition & Behavior Tests
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Respiratory Disease Animal Models
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Ovalbumin-induced allergic asthma is a widely used model to reproduce the airway eosinophilia, pulmonary inflammation and elevated IgE levels found during asthma.

The House Dust Mite (HDM) model demonstrates significant relevance for investigating the molecular mechanisms of allergic asthma pathogenesis.
LPS lung inflammation... Show more »

Ovalbumin-induced allergic asthma is a widely used model to reproduce the airway eosinophilia, pulmonary inflammation and elevated IgE levels found during asthma.

The House Dust Mite (HDM) model demonstrates significant relevance for investigating the molecular mechanisms of allergic asthma pathogenesis.
LPS lung inflammation models are widely used to study host-response patterns in the pulmonary compartment.
Bleomycin induced lung injury model in mice is used for studying idiopathic pulmonary fibrosis (IPF).

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LPS
OVA Asthma
Allergic Asthma
Lipoplysaccharide Lung
Fibrosis-Belomycin
Clinical Chemistry
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Cell-Based Assays
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Screening and mechanism of action studies using primary or immortalized cell lines. Our scientists development experience crosses a broad range of study targets. Customizing each study for sponsor relevance including chosen cell system, stimulant time point(s), and read-outs.

Screening and mechanism of action studies using primary or immortalized cell lines. Our scientists development experience crosses a broad range of study targets. Customizing each study for sponsor relevance including chosen cell system, stimulant time point(s), and read-outs.

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Custom Assays
PBMC
human primary cells
immortalized cell lines
ex vivo primary cells
CNS/Neurology Animal Models
Price on request

Offering validated models for neurodegenerative diseases including Parkinson's and neuro-inflammatory diseases such as multiple sclerosis (MS) with additional capabilities in stroke and cerebral ischemia. Our laboratories are also proud to be known as a center of excellence in the study of neuropathic, chronic and acute pain,... Show more »

Offering validated models for neurodegenerative diseases including Parkinson's and neuro-inflammatory diseases such as multiple sclerosis (MS) with additional capabilities in stroke and cerebral ischemia. Our laboratories are also proud to be known as a center of excellence in the study of neuropathic, chronic and acute pain, offering multiple validated models and relevant pain read-outs. Our pain services are extended with unique pig translational models complementing our rodent studies and offering confidence as compounds move to IND and clinical stages of their development.

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60HDA
Myelin Oligodentrocyte Glycoprotein
Proteolipid Protein
Myelin Basic Protein
Middle Cerebral Artery Occlusion (MCAo)
Four Vessel Occlusion (4VO)
PLP
6-Hydroxyldopamine
In vitro Disease Models
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Dermatology Animal Models
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Dermal research with a host of preclinical and translational models. Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, as well as passive cutaneous anaphylaxis form the stable of our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry

Dermal research with a host of preclinical and translational models. Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, as well as passive cutaneous anaphylaxis form the stable of our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry

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Imiquimod-induced psoriasis
IL-17
IL-23
In vivo Assays
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RNA Sequencing
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Bone/Cartilage Animal Models
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Functional & Cell Type Specific Assays
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Biomarkers
Price on request

Our highly-experienced team of biomarker experts applies systematic, scientifically-rigorous approaches to the discovery and development of biomarker candidates to provide solutions to complex translational questions.

Our highly-experienced team of biomarker experts applies systematic, scientifically-rigorous approaches to the discovery and development of biomarker candidates to provide solutions to complex translational questions.

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Biology
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Animal Physiology Analyses
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Pharmacology & Toxicology
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Hit Identification
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Inflammation Animal Models
Price on request

We offer validated models of many inflammatory diseases including skin (IL-23 psoriasis, dermatitis, delayed type hyper-sensitivity, anaphylaxis), gastro-intestinal (TNBS or DSS induced IBD), respiratory (acute lung injury, asthma, fibrosis), joint (rheumatoid arthritis) and sepsis (LPS induced)

We offer validated models of many inflammatory diseases including skin (IL-23 psoriasis, dermatitis, delayed type hyper-sensitivity, anaphylaxis), gastro-intestinal (TNBS or DSS induced IBD), respiratory (acute lung injury, asthma, fibrosis), joint (rheumatoid arthritis) and sepsis (LPS induced)

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Nucleic Acid Services
Price on request

Nucleic acid extractions from multiple sample types (fresh, frozen & FFPE tissues, liquid biopsies, disaggregated tumor samples, etc)

Nucleic acid extractions from multiple sample types (fresh, frozen & FFPE tissues, liquid biopsies, disaggregated tumor samples, etc)

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PCR
Genomic DNA extraction
Cobas systems (4800 or 6800/8800, b 221, s 201, or u 411)
Nucleic acid extraction
mRNA
miRNA
qPCR Assay
DNA quantification
DNA methylation
RT-qPCR
Real time qPCR
Multiplexed mRNA analysis
Multiplexed miRNA analysis
Tissue Extraction
Applied Biosystems QuantStudio DX
DNA
siRNA
Biochemistry & Molecular Biology
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Gastroenterology Animal Models
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Specialized Cell-Based Assays
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Drug Discovery
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Comprehensive Metabolic Panel
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Glucose Testing
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Genotyping Services
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Genotyping Services

Genotyping Services

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DNA
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DNA Services

DNA Services

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Multiplex Gene Expression Analysis
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Clinical Endocrinology Tests
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Allergy Animal Models
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Alanine Aminotransferase (ALT) Measurement
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Assay Development
Price on request

Assays include human primary cells, immortalized cell lines, murine and rat primary cells, ex vivo primary cells and custom assays.

Assays include human primary cells, immortalized cell lines, murine and rat primary cells, ex vivo primary cells and custom assays.

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Diabetes Animal Models
Price on request

The non-obese diabetic (NOD) mouse model develops spontaneous autoimmune diabetes, similar to type 1 diabetes. The STZ-induced diabetic model involves an autoimmune process that results in the destruction of Langerhans islets. Multiple assessments available, and models available are rat, mouse and pig.

The non-obese diabetic (NOD) mouse model develops spontaneous autoimmune diabetes, similar to type 1 diabetes. The STZ-induced diabetic model involves an autoimmune process that results in the destruction of Langerhans islets. Multiple assessments available, and models available are rat, mouse and pig.

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Oncology Animal Models
Price on request

Various cancer models and specializes in murine solid tumors for both subcutaneous and orthotopic models, with an emphasis on disease progression, biomarkers and immunophenotyping. Other models include metastatic, xenographic and syngeneic.

Various cancer models and specializes in murine solid tumors for both subcutaneous and orthotopic models, with an emphasis on disease progression, biomarkers and immunophenotyping. Other models include metastatic, xenographic and syngeneic.

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Bioanalytical Assays
Price on request

Bioanaylsis for all sample types across all major methodologies.

Bioanaylsis for all sample types across all major methodologies.

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GC-MS
HPLC
LC/MS/MS
Immunoassays
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Analgesic Animal Models
Price on request

A commonly used model to test the effect of new analgesic drugs in post-operative (incisional) pain is the Brennan model. This model can provide good answers to systemic drugs it is less suitable for testing local treatments such as implants, patches, medical devices and creams.

A commonly used model to test the effect of new analgesic drugs in post-operative (incisional) pain is the Brennan model. This model can provide good answers to systemic drugs it is less suitable for testing local treatments such as implants, patches, medical devices and creams.

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Rat Brennan model
pig POP model
T Cell Activation and Proliferation Assays
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Protein Purification and Quantification
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Protein Purification and Quantification Services

Protein Purification and Quantification Services

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Bicinchoninic Acid Assay
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Urinalysis
Price on request

Clinical urine tests are various tests of urine for diagnostic purposes offered by our clinical services.

Clinical urine tests are various tests of urine for diagnostic purposes offered by our clinical services.

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MicroRNA (miRNA) Quantification
Quantitative PCR microRNA quantitation
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Cell-Based Compound Screening
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Human Immune Cell Assays
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Animal Surgery
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RNA Extraction
Price on request

RNA extraction from a wide variety of sample formats

RNA extraction from a wide variety of sample formats

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Preclinical PK/PD Studies
in vivo Pharmacokinetics/Pharmacodynamics
Price on request

A large animal model of neuropathic and nociceptive pain for preclinical evaluation of analgesic or neuro-protective therapies. The sciatica model was developed in the pig, providing more clinically relevant data at the preclinical stages. The model is suitable for evaluating both efficacy and PK/PD in the same animal and can be... Show more »

A large animal model of neuropathic and nociceptive pain for preclinical evaluation of analgesic or neuro-protective therapies. The sciatica model was developed in the pig, providing more clinically relevant data at the preclinical stages. The model is suitable for evaluating both efficacy and PK/PD in the same animal and can be used for systemic, local and cell therapies as well as slow release drugs and devices.

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IBS/IBD Animal Models
Irritable Bowel Syndrome/Disease Animal Models
Price on request

DSS and TNBS-induced; custom protocols available upon request, as are derivative model validations

DSS and TNBS-induced; custom protocols available upon request, as are derivative model validations

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Brown Rat (Rattus norvegicus)
Mouse
colitis
DSS- induced colitis
TNBS- induced colitis
Trinitrobenzenesulfonic acid- induced colitis
Dextran Sulfate Sodium- induced colitis
gastrointestinal
inflammatory Bowel Disease
Inflammation
Gastroenterology
Ulcerative colitis
Crohn's Disease
Microbiology
Pharmacology
Price on request

Within our laboratory services, our pharmacology services include specialized diagnostic testing, clinical trial testing, GLP and GMP assessments of compounds/biologics and more! Our goal is to facilitate the advancement of diagnostic capabilities and improve analytical methods through application of rigorous biomarker-based strategies.

Within our laboratory services, our pharmacology services include specialized diagnostic testing, clinical trial testing, GLP and GMP assessments of compounds/biologics and more! Our goal is to facilitate the advancement of diagnostic capabilities and improve analytical methods through application of rigorous biomarker-based strategies.

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Immunophenotyping
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Cytometry
Price on request

Cytometry Services

Cytometry Services

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Flow Cytometry
Price on request

Multiparametric (16+ color) on the BD LSR Fortessa instrument

Multiparametric (16+ color) on the BD LSR Fortessa instrument

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Brown Rat (Rattus norvegicus)
Human
Mouse
Pig
BD LSRFortessa
Flow cytometry
Cardiovascular
immunology
Autoimmune
Oncology
Immunooncology
inflammation
NASH (Non-Alcoholic Steatohepatitis)
Immunophenotyping
Non-human primates
NAFLD
immunology
Liver Disease
CNS/neurology
antibody validation
Canine
Feline
Miltenyi MacsQuant
Experimental Autoimmune Encephalomyelitis (EAE) Animal Models
Price on request

Variety of highly-validated EAE models with multiple induction methods, customizable protocols and advanced analytical readout capabilities

Variety of highly-validated EAE models with multiple induction methods, customizable protocols and advanced analytical readout capabilities

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Brown Rat (Rattus norvegicus)
Mouse
Rat
Immunology
neurology
CNS/Neurology
multiple sclerosis
MOG- induced Experimental Autoimmune Encephalomyelitis (EAE)
Inflammation
autoimmune
demyelination
remyelination
MBP-induced Experimental Autoimmune Encephalomyelitis (EAE)
PLP-induced Experimental Autoimmune Encephalomyelitis (EAE)
Myelin Oligodentrocyte Glycoprotein-Induced EAE
Myelin Basic Protein-induced EAE
Proteolipid Protein-induced EAE
Spinal Cord Homogenate-induced EAE
SCH-induced EAE
Ex Vivo Models and Assays
Price on request

Ex Vivo Models and Assays Services

Ex Vivo Models and Assays Services

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Cell Signaling Analyses
Price on request

Cell Signaling Analyses Services

Cell Signaling Analyses Services

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Cell Viability & Proliferation Assays
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Cell Viability & Proliferation Assays Services

Cell Viability & Proliferation Assays Services

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Cell Invasion & Migration Assays
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Cell Invasion & Migration Assays Services

Cell Invasion & Migration Assays Services

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Transfection
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Transfection Services

Transfection Services

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Mammalian Cell Culture
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Mammalian Cell Culture Services

Mammalian Cell Culture Services

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Bioparticle Technology
Price on request

Bioparticle Technology Services

Bioparticle Technology Services

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Animal Anxiety and Depression Tests
Price on request

Animal Anxiety and Depression Tests Services

Animal Anxiety and Depression Tests Services

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Animal Motor Function Tests
Price on request

Animal Motor Function Tests Services

Animal Motor Function Tests Services

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Drug Absorption Studies
Price on request

Drug Absorption Studies Services

Drug Absorption Studies Services

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In vitro ADME
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In vitro ADME Services

In vitro ADME Services

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Protein Expression Visualization
Price on request

Protein Expression Visualization Services

Protein Expression Visualization Services

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Next Generation Sequencing (NGS)
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Next Generation Sequencing (NGS) Services

Next Generation Sequencing (NGS) Services

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DNA Sequencing
Price on request

DNA Sequencing Services

DNA Sequencing Services

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Mammalian Models
Price on request

Mammalian Models Services

Mammalian Models Services

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Model Organisms
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Model Organisms Services

Model Organisms Services

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Formulation Services
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Formulation Services

Formulation Services

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Clinical Hematology
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Clinical Hematology Services

Clinical Hematology Services

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Common Histochemical Stains
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Common Histochemical Stains Services

Common Histochemical Stains Services

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Histological Staining
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Histological Staining Services

Histological Staining Services

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Clinical and Anatomic Pathology
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Clinical and Anatomic Pathology Services

Clinical and Anatomic Pathology Services

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Primary Cell Culture
Price on request

Primary Cell Culture Services

Primary Cell Culture Services

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Cell and Tissue Culture
Price on request

Cell and Tissue Culture Services

Cell and Tissue Culture Services

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Human Primary Cells
Price on request

Human Primary Cells Services

Human Primary Cells Services

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Animal Biospecimens
Price on request

Animal Biospecimens Services

Animal Biospecimens Services

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Tissue Sectioning
Price on request

Tissue Sectioning Services

Tissue Sectioning Services

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Tissue Embedding
Price on request

Tissue Embedding Services

Tissue Embedding Services

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Tissue Processing
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Tissue Processing Services

Tissue Processing Services

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Human Whole Blood & PBMCs
Price on request

Human Whole Blood & PBMCs Services

Human Whole Blood & PBMCs Services

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Human Biofluids
Price on request

Human Biofluids Services

Human Biofluids Services

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Human Biospecimens
Price on request

Human Biospecimens Services

Human Biospecimens Services

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Biospecimens
Price on request

Biospecimens Services

Biospecimens Services

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Biomolecular Interaction Analysis
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Biomolecular Interaction Analysis Services

Biomolecular Interaction Analysis Services

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Oxidoreductase Assays
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Oxidoreductase Assays Services

Oxidoreductase Assays Services

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Enzyme Assays
Price on request

Enzyme Assays Services

Enzyme Assays Services

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Biochemical Assays
Price on request

Biochemical Assays Services

Biochemical Assays Services

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Nuclear Imaging
Price on request

Nuclear imaging Services

Nuclear imaging Services

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Radiography
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Radiography Services

Radiography Services

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Imaging & Spectroscopy
Price on request

Imaging & Spectroscopy Services

Imaging & Spectroscopy Services

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