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MD Biosciences Preclinical Laboratory Services

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Oakdale, Minnesota, US

About MD Biosciences Preclinical Laboratory Services

Founded: 1991 Type: Privately Held Size: 51-200 employees Divison of: MD Biosciences

MD Biosciences Preclinical Laboratory Services is a niche preclinical research group working with biopharmacuetical and medical device companies. Our core therapeutic areas are oncology, inflammation, CNS, pain and cardiovascular with an emphasis on the interplay between these systems.

We place a great deal... Show more »

MD Biosciences Preclinical Laboratory Services is a niche preclinical research group working with biopharmacuetical and medical device companies. Our core therapeutic areas are oncology, inflammation, CNS, pain and cardiovascular with an emphasis on the interplay between these systems.

We place a great deal of importance on the scientific rationale of each individual study to ensure that it meets the goals of the R&D program. We are willing to invest in custom study design, development and validation with our sponsors to achieve clinically relevant data at the preclinical stages. We feel that this will help reduce the failure rate in clincial stages and the burden upon the industry.

We employ a highly talented group of scientists consisting of PhD, MSc and Bachelor degrees. Each scientist is specialized and highly skilled in their area of expertise. Our group has all been trained in various techniques including microsurgical procedures, injection methods, model and method development as well as imaging and histological techniques.

Selected Publications

  • Castel, D., Willentz, E., Doron, O., Brenner, O. and Meilin, S. (2014), Characterization of a porcine model of post-operative pain. European Journal of Pain, 18: 496–505. doi: 10.1002/j.1532-2149.2013.00399.x
  • Meilin, S., Machicao, F., & Elmlinger, M. (2014). Treatment with Actovegin improves spatial learning and memory in rats following transient forebrain ischaemia. Journal of Cellular and Molecular Medicine (8), 1623–1630. doi:10.1111/jcmm.12297
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Certifications & Qualifications

AAALAC

Our Services (198)


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Neurochemistry Animal Models

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Drug Development

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Hair Growth Animal Models

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Flow Cytometry Imaging

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Flow Cytometry Experimental Design

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Flow Cytometry Data acquisition

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Flow Cytometry Data Analysis

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FACS

Fluorescence Activated Cell Sorting
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Cell Cycle Analysis

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Custom CNS Animal Models

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Neurodegenerative diseases like Parkinson's as well as neuro-inflammatory diseases such as multiple sclerosis (MS), with additional capabilities in stroke and cerebral ischemia. Offering customizable study options upon request.

Neurodegenerative diseases like Parkinson's as well as neuro-inflammatory diseases such as multiple sclerosis (MS), with additional capabilities in stroke and cerebral ischemia. Offering customizable study options upon request.

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Experimental Autoimmune Encephalomyelitis (EAE) induced- neuropathy 6-OHDA- induced parkinson model MPTP-induced Parkinson's disease model PLP-induced Experimental Autoimmune Encephalomyelitis (EAE) Spinal Cord Homogenate-induced EAE

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Chronic Constriction Injury Animal Models

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the left sciatic nerve is ligated causing inflammation and swelling, and ultimately peripheral pain.

the left sciatic nerve is ligated causing inflammation and swelling, and ultimately peripheral pain.

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sprague dawley rat c57/B16 mouse

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Cell-Based Screening Methods

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Cell Signaling Assays

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Cell Death Assays

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Colony Formation Assays

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Cell Adhesion Assays

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BrdU Assay

Bromodeoxyuridine Assay
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Cell Migration Assays

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Electroporation

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Cell Co-Cultivation

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Extracellular Vesicle Analysis

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Electrophysiology

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Dantec KEYPOINT Focus tool utilized to measure electrophysiological action potentials. Capabilities in the assessment of muscle, motor and sensory action potential in rodent models of peripheral nerve injury to measure nerve regeneration, degeneration and protection in response to various treatments. Can apply to non-rodent species and pig models.

Dantec KEYPOINT Focus tool utilized to measure electrophysiological action potentials. Capabilities in the assessment of muscle, motor and sensory action potential in rodent models of peripheral nerve injury to measure nerve regeneration, degeneration and protection in response to various treatments. Can apply to non-rodent species and pig models.

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Custom Animal Models

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Forced Swim Test

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A behavioral test used on rats to assess responses to threat of drowning

A behavioral test used on rats to assess responses to threat of drowning

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Exploratory Animal Studies

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Elevated Plus Maze Animal Anxiety Test

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A test measuring anxiety in laboratory animals that usually uses rodents as a screening test

A test measuring anxiety in laboratory animals that usually uses rodents as a screening test

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Cylinder Test

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Asymmetry analysis assesses behavioral deficits in rats after stroke or brain injury.

Asymmetry analysis assesses behavioral deficits in rats after stroke or brain injury.

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Dose Range Finding (DRF) Studies

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Quantitative Western Blot Analysis

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PCR Primer Design

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PCR Product Purification

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Porcine Models

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Large animal models offer high translatability from preclinical research to clinical trials. We offer pig models in neuropathic pain, post-operative pain, wound healing and other dermatology studies and pig nerve block.

Large animal models offer high translatability from preclinical research to clinical trials. We offer pig models in neuropathic pain, post-operative pain, wound healing and other dermatology studies and pig nerve block.

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Patient Derived Cancer Cell Models

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Pulmonary Inflammation Animal Models

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MD Biosciences offers models to study the inflammatory components involved in lung inflammation and asthma including OVA-induced asthma, HDM-induced asthma, LPS-acute lung injury, and bleomycin-induced fibrosis.

MD Biosciences offers models to study the inflammatory components involved in lung inflammation and asthma including OVA-induced asthma, HDM-induced asthma, LPS-acute lung injury, and bleomycin-induced fibrosis.

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Mouse LPS Rat LPS OVA Asthma Bleomycin-induced Scleroderma/Systemic Sclerosis

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Peritonitis Animal Models

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Polymorphism Screening

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Polymorphism

Polymorphism

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Prothrombin Time Test

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Partial Thromboplastin Time Test

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Prolactin Testing

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Progesterone Testing

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Pregnancy Testing

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Parathyroid Hormone Testing

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Periodic Acid-Schiff (PAS) Staining

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Preclinical Histology

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Primary Cell Isolation

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Primary Biopsy Culture

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Primary Cell Lines

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Porcine Dermis

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Plastic Methyl Methacrylate Embedding

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Paraffin Sectioning

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Paraffin Embedding

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PBMC Isolation

Peripheral Blood Mononuclear Cell Isolation
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Peptide Scanning

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Plasma Bioanalysis

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Peroxidase Assays

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In vivo Drug Efficacy Testing

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in vitro, in vivo and ex vivo modeling services for efficacy determinations, PK/PD assessments, mechanism of action studies, compound screening, biomarker analysis and more!

in vitro, in vivo and ex vivo modeling services for efficacy determinations, PK/PD assessments, mechanism of action studies, compound screening, biomarker analysis and more!

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Brown Rat (Rattus norvegicus) Pig murine CNS/Neurology Pain Metabolism and Endocrinology Inflammation Cancer Arthritis Rheumatoid Arthritis Fibrosis Obesity Sepsis Diabetes Neurodegenerative diseases Neuropathy Experimental autoimmune encephalomyelitis (EAE) Colitis Dermatitis Psoriasis Inflammatory Bowel Disease Cystic fibrosis Asthma Multiple sclerosis (MS) Pulmonary fibrosis Parkinson's disease (PD) Acute Kidney Injury Collagen antibody-induced arthritis Renal Cancer Show 29 more tags Show less

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Preclinical Study Design

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In vivo Pharmacology Studies

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MD Biosciences features a wide range of validated in vivo models of human disease to support preclinical drug efficacy tests, and we provide customizable disease-specific models in order to fit each study's needs.

MD Biosciences features a wide range of validated in vivo models of human disease to support preclinical drug efficacy tests, and we provide customizable disease-specific models in order to fit each study's needs.

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ADME/DMPK Studies

Drug Metabolism and Pharmacokinetics
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Animal Model in vivo Analyses

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Variety of preclinical animal models for pharmacology, efficacy and mechanism of action studies in addition to biomarker analysis

Variety of preclinical animal models for pharmacology, efficacy and mechanism of action studies in addition to biomarker analysis

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Mouse Pig Rat Transgenic rat Transgenic mouse Non-Alcoholic Steatohepatitis (NASH) Collagen-induced arthritis DSS- induced colitis Diet-induced obesity DNCB Induced Contact Dermatitis tail flick visceral Capsaicin bilateral AKI HDM Rat IMQ Mouse IMQ Collagen antibody induced rheumatoid arthritis pig POP model pig neuropathic pain Il-23-induced Psoriasis Fluorescein Isothiocynate Dermatitis Oxazalone Delayed Type Hypertrophy Passive Cutaneous Anaphylaxis OVA Asthma Allergic Asthma Lipoplysaccharide Lung Fibrosis-Belomycin Collagen-induced rheumatoid arthritis Adjuvant-Induced rheumatoid arthritis Myelin Oligodentrocyte Glycoprotein Proteolipid Protein Myelin Basic Protein Acute 1-Methyl-4Phenyl-1,2,3,6-Tetrahydropyridine (MPTP) Middle Cerebral Artery Occlusion (MCAo) Four Vessel Occlusion (4VO) Non-Obese Diabetic (NOD) Steptozotocin (STZ) Dextran Sodium Sulfate (DSS) 2,4,6-trinitrobenzenesulfonic acid (TNBS) Unilateral Kidney Injury (AKI) Sepsis Acute Kidney Injury (AKI) Chronic Constrictive Injury (CCI) Spinal Nerve Ligation (SNL) Cuff Crush Streptozotocin (STZ) Complete Fruend Adjuvant (CFA) Carageenan Monosodium Iodoacetate (MIA) Poly Arthritic Mono Arthritic Brennen Nerve Block Incision Excision Burn SEB-induced T-cell Anergy House dust mite induced asthma Bleomycin-induced Scleroderma/Systemic Sclerosis Non-alcoholic steatohepatitis Renal Fibrosis Fibrosis Show 63 more tags Show less

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High Throughput Screening (HTS)

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Biomarker Analysis

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Variety of methods across all major platforms for biomarker identification, development, analysis, qualification, validation and downstream application

Variety of methods across all major platforms for biomarker identification, development, analysis, qualification, validation and downstream application

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Human Mouse Pig Rat Dog Large animals Cat Nonhuman Primate Flow cytometry Immunoassays ELISA Immunofluorescence qPCR western blot immunohistochemistry IHC Triple-stain Immunohistochemistry Dual-stain Immunohistochemistry Cell-based assays Immunogenicity Studies Immunophenotyping qPCR Assay ELISA-based assay Western blotting Epigenetics multiplex Western blot western blot normalization western blot optimization quantitative western blot ELISA Protein Analysis Real time qPCR Clinical chemistry FFPE tissue Mammalian tissue Frozen Tissue Diseased human tissue Normal Human tissue tissue section Tissue Block Fresh Tissue Fresh Tumors whole tissue Show 42 more tags Show less

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Biomarker Discovery

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Our capabilities include support for projects at every stage in the development pipeline, from the initial biomarker identification, qualification and validation through to downstream clinical applications. Some services include identification of druggable targets, characterization of indicators of disease severity or stratification of patient populations.

Our capabilities include support for projects at every stage in the development pipeline, from the initial biomarker identification, qualification and validation through to downstream clinical applications. Some services include identification of druggable targets, characterization of indicators of disease severity or stratification of patient populations.

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Hematology Flow cytometry assay development Western blotting ELISA Protein Analysis Multiplexed Protein Analysis Serology Real time qPCR Multiplexed mRNA analysis Multiplexed miRNA analysis Tissue Extraction Urinalysis Clinical chemistry Show 13 more tags Show less

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Animal Behavior Studies

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Animal Model Housing and Maintenance

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Demyelination Animal Models

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experimental autoimmune encephalomyelitis (EAE) and MS, EAE has been used as a model for the study of human demyelinating disease.

experimental autoimmune encephalomyelitis (EAE) and MS, EAE has been used as a model for the study of human demyelinating disease.

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Autoimmune Disease Animal Models

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proliferation Cellular Immunity Non-specific Immunity Humoral Immunity Allergy/Hypersensitivity Th17 activation T cell inhibition Cytokine Release T cell stimulation Macrophage (Inhibition) Macrophage (stimulation) Show 11 more tags Show less

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Combination Therapy Efficacy Testing in Mice

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Troponin Assay

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ELISA

Enzyme-linked immunosorbent assay
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plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones

plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones

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Acute Inflammatory Pain Animal Models

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offers commonly used models of acute inflammatory pain such as CFA and carrageenan - that can be extended - to evaluate a more chronic inflammatory pain state. We can help you study the release of Cytokines and their role in the initiation and maintenance of the inflammatory response along with prostaglandin

offers commonly used models of acute inflammatory pain such as CFA and carrageenan - that can be extended - to evaluate a more chronic inflammatory pain state. We can help you study the release of Cytokines and their role in the initiation and maintenance of the inflammatory response along with prostaglandin

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CFA Carrageenan

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Visceral Pain Animal Models

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Used in nociceptive pain models

Used in nociceptive pain models

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IHC Biomarker analysis PK von frey Motor Function

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Osteoarthritis Animal Models

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Monosodium iodoacetate (MIA) induced osteoarthritis in rats. Cartilage damage and degradation leads to a chronic neuronal damage with neuropathic characteristics.

Monosodium iodoacetate (MIA) induced osteoarthritis in rats. Cartilage damage and degradation leads to a chronic neuronal damage with neuropathic characteristics.

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histology Open field Rotorod joint pain weight bearing Thermal Hyperalgesia mechanical Allodynia paw edema joint compression Show 9 more tags Show less

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Psoriasis Animal Models

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Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, and passive cutaneous anaphylaxis are our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry.

Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, and passive cutaneous anaphylaxis are our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry.

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Imiquimod-induced psoriasis

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Atopic Dermatitis Animal Models

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FITC Induced Contact Dermatitis DNCB Induced Contact Dermatitis

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Custom Pain Animal Models

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Animal Nociception Tests

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MD Biosciences offers a wide range of pre-clinical pain models for nociception that can be customized according to sponsors criteria. Validated models include tail flick, visceral and capsaicin.

MD Biosciences offers a wide range of pre-clinical pain models for nociception that can be customized according to sponsors criteria. Validated models include tail flick, visceral and capsaicin.

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tail flick visceral Capsaicin

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Ischemia Animal Models

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Focal ischemia (MCAo) models are commonly used to mimic the human stroke situation, however the global ischemia model (4VO) would be highly relevant in clinical conditions such as cardiac arrest.

Focal ischemia (MCAo) models are commonly used to mimic the human stroke situation, however the global ischemia model (4VO) would be highly relevant in clinical conditions such as cardiac arrest.

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MCAo 4Vo

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In vitro Diabetes Models

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The non-obese diabetic (NOD) mouse model develops spontaneous autoimmune diabetes, which shares similarities with type 1 diabetes. The STZ-induced diabetic model involves an autoimmune process that results in the destruction of Langerhans islets.

The non-obese diabetic (NOD) mouse model develops spontaneous autoimmune diabetes, which shares similarities with type 1 diabetes. The STZ-induced diabetic model involves an autoimmune process that results in the destruction of Langerhans islets.

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NOD STZ unilateral AKI bilateral AKI DIO rat DIO mouse Show 6 more tags Show less

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Inflammation Assays

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Anaphylaxis Animal Models

PCA Animal Model
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Passive Cutaneous Anaphylaxis (PCA) is an immediate dermal response to an allergan-IgE interaction. It is based on a passively transferred anaphylactic reaction from antiserum. Assessments include dry extravasation (mm), absorbancy, PK blood analysis and biomarker analysis.

Passive Cutaneous Anaphylaxis (PCA) is an immediate dermal response to an allergan-IgE interaction. It is based on a passively transferred anaphylactic reaction from antiserum. Assessments include dry extravasation (mm), absorbancy, PK blood analysis and biomarker analysis.

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Delayed-Type Hypersensitivity (DTH) Animal Models

Delayed Type Hypersensitivity Animal Models
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Delayed Type Hypersensitivity (DTH) model evaluates the cell-mediated immune response to repeated exposure of skin to oxazolone. This results in a DTH reaction involving CD4+, CD8+ T cells and macrophages, resulting in local skin inflammation characterized by an increased thickness of the skin.

Delayed Type Hypersensitivity (DTH) model evaluates the cell-mediated immune response to repeated exposure of skin to oxazolone. This results in a DTH reaction involving CD4+, CD8+ T cells and macrophages, resulting in local skin inflammation characterized by an increased thickness of the skin.

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Contact Dermatitis Animal Models

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FITC & DNCB-induced Contact Dermatitis Models. These models share many of the features of human contact dermatitis, both sensitization and challenge occurs epicutaneously.

FITC & DNCB-induced Contact Dermatitis Models. These models share many of the features of human contact dermatitis, both sensitization and challenge occurs epicutaneously.

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FITC Induced Contact Dermatitis DNCB Induced Contact Dermatitis

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In vivo Psoriasis Assay

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IMQ-Induced Psoriasis Assay

This rapid model of psoriasis-like inflammation involves the IL-17/IL-23 axis. Imiquimod is a potent TLR7/8 ligand and potent immune modulator. Application of imiquimod on the back and ear of mice induces a psoriasis-like inflammation.

IMQ-Induced Psoriasis Assay

This rapid model of psoriasis-like inflammation involves the IL-17/IL-23 axis. Imiquimod is a potent TLR7/8 ligand and potent immune modulator. Application of imiquimod on the back and ear of mice induces a psoriasis-like inflammation.

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Rat IMQ Mouse IMQ

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Lung Inflammation Animal Models

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Following induction by the intranasal administration of LPS, leukocytes (mainly neutrophils) are recruited to the lung. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.

Following induction by the intranasal administration of LPS, leukocytes (mainly neutrophils) are recruited to the lung. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.

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LPS LPS

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Colitis Animal Models

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Acute DSS-induced colitis in mice and rats
Chronic DSS-induced colitis in mice
TNBS-induced colitis in mice and rats

Acute DSS-induced colitis in mice and rats
Chronic DSS-induced colitis in mice
TNBS-induced colitis in mice and rats

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Mouse Rat

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Rheumatoid Arthritis Animal Models

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Collagen induced arthritis (CIA) and adjuvant induced arthritis (AIA) are two commonly used models for this purpose. At MD Biosciences we offer our clients the novel and rapid collagen antibody-induced arthritis (CAIA) model as well.

Collagen induced arthritis (CIA) and adjuvant induced arthritis (AIA) are two commonly used models for this purpose. At MD Biosciences we offer our clients the novel and rapid collagen antibody-induced arthritis (CAIA) model as well.

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Collagen-induced arthritis Collagen antibody induced rheumatoid arthritis

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Global Ischemia 4VO Animal Model

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The Global Ischemia model involves permanent occlusion of vertebral arteries and transient occlusion of carotid arteries. The 4VO model can be used to evaluate not only lack of blood flow to brain as found in cardiac arrest and coronary bypass surgery, but also neurodegeneration, dementia and oxygen stress.

The Global Ischemia model involves permanent occlusion of vertebral arteries and transient occlusion of carotid arteries. The 4VO model can be used to evaluate not only lack of blood flow to brain as found in cardiac arrest and coronary bypass surgery, but also neurodegeneration, dementia and oxygen stress.

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Rat permanent 4VO Rat transient 4VO

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Sepsis Animal Models

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LPS is used to induce sepsis in mice, setting off an immune response activating immune cells that excrete cytokines.

LPS is used to induce sepsis in mice, setting off an immune response activating immune cells that excrete cytokines.

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Sepsis induced by LPS Mouse LPS

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Focal Cerebral Ischemia Animal Models

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The focal cerebral ischemic model is commonly used to assess ischemic injury in the brain, evaluate stroke or neurodegeneration as a result of oxygen stress.

The focal cerebral ischemic model is commonly used to assess ischemic injury in the brain, evaluate stroke or neurodegeneration as a result of oxygen stress.

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Mouse transient MCAo Rat transient MCAo Rat permanent MACo

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Postoperative Pain Animal Models

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Validated POP and Brennan models using pigs and mice. Pigs demonstrate notable resemblance to human anatomy & physiology. This includes analogous dermal epidermal thickness, dermal papillary bodies and abundant subdermal adipose tissue to humans. Functionally, pig and human are similar in epidermal turnover time, type of keratinous proteins and lipid composition of the stratum corneum.

Validated POP and Brennan models using pigs and mice. Pigs demonstrate notable resemblance to human anatomy & physiology. This includes analogous dermal epidermal thickness, dermal papillary bodies and abundant subdermal adipose tissue to humans. Functionally, pig and human are similar in epidermal turnover time, type of keratinous proteins and lipid composition of the stratum corneum.

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Rat Brennan model pig POP model

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Asthma Animal Models

Price on request

The murine OVA-induced asthma model is a widely used model that results in the characteristic features of asthma allowing the study and assessment of novel treatments.
he House Dust Mite (HDM) murine model of allergic asthma is used to assess therapies with potential indications in airway inflammation and/or pulmonary... Show more »

The murine OVA-induced asthma model is a widely used model that results in the characteristic features of asthma allowing the study and assessment of novel treatments.
he House Dust Mite (HDM) murine model of allergic asthma is used to assess therapies with potential indications in airway inflammation and/or pulmonary inflammation driven primarily by repeated exposure to allergens. Model includes Th2- associated cytokine response, eosinophilic infiltration into the lungs, airway hyper responsiveness (AHR) and epithelial tissue remodeling.

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OVA HDM LPS

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Nociceptive Pain Animal Models

Price on request

MD Biosciences offers a wide range of pre-clinical pain models for nociception that can be customized according to sponsors criteria.

MD Biosciences offers a wide range of pre-clinical pain models for nociception that can be customized according to sponsors criteria.

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tail flick visceral Capsaicin

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Nerve Block Animal Models

Price on request

The rapid preclinical model of sciatic nerve block enables researchers to directly access and dose to the sciatic nerve and allows the screening of new nerve blocking agents or drugs designed to reverse the local analgesia. The capability can be applied to other models such as the SNL and CCI models.

The rapid preclinical model of sciatic nerve block enables researchers to directly access and dose to the sciatic nerve and allows the screening of new nerve blocking agents or drugs designed to reverse the local analgesia. The capability can be applied to other models such as the SNL and CCI models.

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rat model pig model

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Neuropathic Pain Animal Models

Price on request

Models in both the rodent as well as the pig bringing much needed translational value from preclinical studies to the human situation.

Models in both the rodent as well as the pig bringing much needed translational value from preclinical studies to the human situation.

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nerve injury chemotherapy STZ diabetic pig neuropathic pain

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Myocardial Ischemia Reperfusion Injury Animal Models

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ligation induced acute cardiac infarction Coil-induced acute cardiac infarction multi-focal myocardial infarction balloon-induced acute cardiac reperfusion Induction of vascular disease

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Chemotherapy-Induced Peripheral Neuropathy Animal Models

Price on request

We conduct preclinical neuropathy models induced with Vincristine or Taxol to mimic the peripheral neuropathy that often results from chemotherapy treatments.

We conduct preclinical neuropathy models induced with Vincristine or Taxol to mimic the peripheral neuropathy that often results from chemotherapy treatments.

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Rat taxol Rat vincristine

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Acute Kidney Failure Models

Price on request

MD Biosciences has validated ischemia-induced renal injury models (unilateral and bilateral) and has familiarity with a variety of other methods should Sponsors have interest in performing validation work on a cost-share basis.

MD Biosciences has validated ischemia-induced renal injury models (unilateral and bilateral) and has familiarity with a variety of other methods should Sponsors have interest in performing validation work on a cost-share basis.

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LPS Mouse LPS Rat LPS Sepsis Acute Kidney Injury (AKI) Unilateral Kidney Injury Bilateral Acute Kidney Injury Show 6 more tags Show less

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Parkinsons Disease Animal Models

Price on request

We offer the classic 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)- and 6-hydroxydopamine (6-OHDA)-induced neurotoxin models and genetic models of the disease.

We offer the classic 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)- and 6-hydroxydopamine (6-OHDA)-induced neurotoxin models and genetic models of the disease.

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acute MPTP 60HDA

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Spinal Cord Injury Models

Price on request

MDB offers a validated neurite outgrowth assay provides an in vitro method to examine the potential effects of exogenous compounds on neuronal activity and development, providing valuable information on the potential of therapeutic compounds to either enhance or inhibit neuritogenesis.

MDB offers a validated neurite outgrowth assay provides an in vitro method to examine the potential effects of exogenous compounds on neuronal activity and development, providing valuable information on the potential of therapeutic compounds to either enhance or inhibit neuritogenesis.

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neurite outgrowth

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In vivo Phenotypic Screening

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LPS-Induced Inflammation Animal Models

Lipopolysaccharide-Induced Inflammation Animal Models
Price on request

The LPS lung inflammation model is an acute lung inflammation model where researchers can observe the pulmonary response following exposure to bacteria. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.

The LPS lung inflammation model is an acute lung inflammation model where researchers can observe the pulmonary response following exposure to bacteria. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.

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F4/80 microglia cells GFAP

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Wound Healing Animal Models

Price on request

MD Biosciences offers various models of wound healing in both rodents and pigs. While rodent models provide data for earlier research stages, pig models are ideally suited for wound healing efficacy studies.

MD Biosciences offers various models of wound healing in both rodents and pigs. While rodent models provide data for earlier research stages, pig models are ideally suited for wound healing efficacy studies.

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mouse incisional wound rat incisional wound rodent burn wound pig incisional wound pig excisional wound pig burn wound Show 6 more tags Show less

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Custom Cell-Based Assay Development

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Custom Assays PBMC human primary cells immortalized cell lines murine rat ex vivo primary cells Show 7 more tags Show less

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Obesity Animal Models

Price on request

We conduct metabolic and inflammation research, with diet-induced rat and mouse models, and including the following assessments: feed intake, body weight, insulin levels, glucose tolerance, blood pressure, feed preparing, and biomarkers.

We conduct metabolic and inflammation research, with diet-induced rat and mouse models, and including the following assessments: feed intake, body weight, insulin levels, glucose tolerance, blood pressure, feed preparing, and biomarkers.

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Diet-induced obesity DIO rat DIO mouse

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Metabolic Animal Models

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Metabolic disease services include models for diabetes, renal, obesity and inflammatory bowel disease. Multiple induction methods may be available for each disease. Diabetes models may be induced with streptozotocin, the use of db/db or NOD mice. Inflammatory Bowel Disease (IBD) may be induced with TNBS or DSS.

Metabolic disease services include models for diabetes, renal, obesity and inflammatory bowel disease. Multiple induction methods may be available for each disease. Diabetes models may be induced with streptozotocin, the use of db/db or NOD mice. Inflammatory Bowel Disease (IBD) may be induced with TNBS or DSS.

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obesity diabetes acute kidney injury IBD

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Clinical Laboratory Services

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MD Biosciences is proud to offer comprehensive diagnostic services for sample analysis and assay development performed to CLIA and GLP regulatory compliance. We provide a wide range of services for specialized diagnostic testing, clinical trial testing, GLP and GMP assessments of compounds/biologics and more! Our goal is to... Show more »

MD Biosciences is proud to offer comprehensive diagnostic services for sample analysis and assay development performed to CLIA and GLP regulatory compliance. We provide a wide range of services for specialized diagnostic testing, clinical trial testing, GLP and GMP assessments of compounds/biologics and more! Our goal is to facilitate the advancement of diagnostic capabilities and improve analytical methods through application of rigorous biomarker-based strategies.

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Hematology Flow cytometry assay development real-time PCR Western blotting ELISA Protein Analysis Multiplexed Protein Analysis Serology Multiplexed mRNA analysis Multiplexed miRNA analysis Tissue Extraction Urinalysis Clinical chemistry Show 13 more tags Show less

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Cardiovascular Animal Models

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Animal Learning, Memory, and Behavior Tests

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Immunology Animal Models

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Cellular Immunity Non-specific Immunity Humoral Immunity Allergy/Hypersensitivity

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Safety Pharmacology

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Drug Discovery & Development

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Cells and Tissues

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Infectious Disease Animal Models

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RNA Services

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Bioanalysis

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Bioanaylsis for all sample types across all major methodologies.

Bioanaylsis for all sample types across all major methodologies.

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Protein Quantification

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Nephrology Animal Models

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Immune Cell Assays

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Protein Services

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Functional Human Tissue Assays

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Animal Cognition & Behavior Tests

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Respiratory Disease Animal Models

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Ovalbumin-induced allergic asthma is a widely used model to reproduce the airway eosinophilia, pulmonary inflammation and elevated IgE levels found during asthma.

The House Dust Mite (HDM) model demonstrates significant relevance for investigating the molecular mechanisms of allergic asthma pathogenesis.
LPS lung inflammation... Show more »

Ovalbumin-induced allergic asthma is a widely used model to reproduce the airway eosinophilia, pulmonary inflammation and elevated IgE levels found during asthma.

The House Dust Mite (HDM) model demonstrates significant relevance for investigating the molecular mechanisms of allergic asthma pathogenesis.
LPS lung inflammation models are widely used to study host-response patterns in the pulmonary compartment.
Bleomycin induced lung injury model in mice is used for studying idiopathic pulmonary fibrosis (IPF).

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LPS OVA Asthma Allergic Asthma Lipoplysaccharide Lung Fibrosis-Belomycin

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Clinical Chemistry

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Cell-Based Assays

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Screening and mechanism of action studies using primary or immortalized cell lines. Our scientists development experience crosses a broad range of study targets. Customizing each study for sponsor relevance including chosen cell system, stimulant time point(s), and read-outs.

Screening and mechanism of action studies using primary or immortalized cell lines. Our scientists development experience crosses a broad range of study targets. Customizing each study for sponsor relevance including chosen cell system, stimulant time point(s), and read-outs.

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Custom Assays PBMC human primary cells immortalized cell lines ex vivo primary cells

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CNS/Neurology Animal Models

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Offering validated models for neurodegenerative diseases including Parkinson's and neuro-inflammatory diseases such as multiple sclerosis (MS) with additional capabilities in stroke and cerebral ischemia. Our laboratories are also proud to be known as a center of excellence in the study of neuropathic, chronic and acute pain,... Show more »

Offering validated models for neurodegenerative diseases including Parkinson's and neuro-inflammatory diseases such as multiple sclerosis (MS) with additional capabilities in stroke and cerebral ischemia. Our laboratories are also proud to be known as a center of excellence in the study of neuropathic, chronic and acute pain, offering multiple validated models and relevant pain read-outs. Our pain services are extended with unique pig translational models complementing our rodent studies and offering confidence as compounds move to IND and clinical stages of their development.

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60HDA Myelin Oligodentrocyte Glycoprotein Proteolipid Protein Myelin Basic Protein Middle Cerebral Artery Occlusion (MCAo) Four Vessel Occlusion (4VO) PLP 6-Hydroxyldopamine Show 8 more tags Show less

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In vitro Disease Models

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Dermatology Animal Models

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Dermal research with a host of preclinical and translational models. Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, as well as passive cutaneous anaphylaxis form the stable of our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry

Dermal research with a host of preclinical and translational models. Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, as well as passive cutaneous anaphylaxis form the stable of our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry

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Imiquimod-induced psoriasis IL-17 IL-23

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In vivo Assays

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Bone/Cartilage Animal Models

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Functional & Cell Type Specific Assays

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Biomarkers

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Our highly-experienced team of biomarker experts applies systematic, scientifically-rigorous approaches to the discovery and development of biomarker candidates to provide solutions to complex translational questions.

Our highly-experienced team of biomarker experts applies systematic, scientifically-rigorous approaches to the discovery and development of biomarker candidates to provide solutions to complex translational questions.

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Biology

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Animal Physiology Analyses

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Pharmacology & Toxicology

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Hit Identification

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Inflammation Animal Models

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We offer validated models of many inflammatory diseases including skin (IL-23 psoriasis, dermatitis, delayed type hyper-sensitivity, anaphylaxis), gastro-intestinal (TNBS or DSS induced IBD), respiratory (acute lung injury, asthma, fibrosis), joint (rheumatoid arthritis) and sepsis (LPS induced)

We offer validated models of many inflammatory diseases including skin (IL-23 psoriasis, dermatitis, delayed type hyper-sensitivity, anaphylaxis), gastro-intestinal (TNBS or DSS induced IBD), respiratory (acute lung injury, asthma, fibrosis), joint (rheumatoid arthritis) and sepsis (LPS induced)

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Nucleic Acid Services

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Nucleic acid extractions from multiple sample types (fresh, frozen & FFPE tissues, liquid biopsies, disaggregated tumor samples, etc)

Nucleic acid extractions from multiple sample types (fresh, frozen & FFPE tissues, liquid biopsies, disaggregated tumor samples, etc)

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Cobas systems (4800 or 6800/8800, b 221, s 201, or u 411) Applied Biosystems QuantStudio DX PCR Genomic DNA extraction Nucleic acid extraction qPCR Assay DNA quantification DNA methylation RT-qPCR Real time qPCR Multiplexed mRNA analysis Multiplexed miRNA analysis Tissue Extraction mRNA miRNA DNA siRNA Show 17 more tags Show less

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Biochemistry & Molecular Biology

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Gastroenterology Animal Models

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Specialized Cell-Based Assays

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Drug Discovery

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Comprehensive Metabolic Panel

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Glucose Testing

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Genotyping Services

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Genotyping Services

Genotyping Services

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DNA Services

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DNA Services

DNA Services

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Multiplex Gene Expression Analysis

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Clinical Endocrinology Tests

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Allergy Animal Models

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Alanine Aminotransferase (ALT) Measurement

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Assay Development

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Assays include human primary cells, immortalized cell lines, murine and rat primary cells, ex vivo primary cells and custom assays.

Assays include human primary cells, immortalized cell lines, murine and rat primary cells, ex vivo primary cells and custom assays.

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Diabetes Animal Models

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The non-obese diabetic (NOD) mouse model develops spontaneous autoimmune diabetes, similar to type 1 diabetes. The STZ-induced diabetic model involves an autoimmune process that results in the destruction of Langerhans islets. Multiple assessments available, and models available are rat, mouse and pig.

The non-obese diabetic (NOD) mouse model develops spontaneous autoimmune diabetes, similar to type 1 diabetes. The STZ-induced diabetic model involves an autoimmune process that results in the destruction of Langerhans islets. Multiple assessments available, and models available are rat, mouse and pig.

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Oncology Animal Models

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Various cancer models and specializes in murine solid tumors for both subcutaneous and orthotopic models, with an emphasis on disease progression, biomarkers and immunophenotyping. Other models include metastatic, xenographic and syngeneic.

Various cancer models and specializes in murine solid tumors for both subcutaneous and orthotopic models, with an emphasis on disease progression, biomarkers and immunophenotyping. Other models include metastatic, xenographic and syngeneic.

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Bioanalytical Assays

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Bioanaylsis for all sample types across all major methodologies.

Bioanaylsis for all sample types across all major methodologies.

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GC-MS HPLC LC/MS/MS

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Immunoassays

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Analgesic Animal Models

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A commonly used model to test the effect of new analgesic drugs in post-operative (incisional) pain is the Brennan model. This model can provide good answers to systemic drugs it is less suitable for testing local treatments such as implants, patches, medical devices and creams.

A commonly used model to test the effect of new analgesic drugs in post-operative (incisional) pain is the Brennan model. This model can provide good answers to systemic drugs it is less suitable for testing local treatments such as implants, patches, medical devices and creams.

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Rat Brennan model pig POP model

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T Cell Activation and Proliferation Assays

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Protein Purification

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Protein Purification and Quantification Services

Protein Purification and Quantification Services

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Bicinchoninic Acid Assay

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Urinalysis

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Clinical urine tests are various tests of urine for diagnostic purposes offered by our clinical services.

Clinical urine tests are various tests of urine for diagnostic purposes offered by our clinical services.

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MicroRNA (miRNA) Quantification

Quantitative PCR microRNA quantitation
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Cell-Based Compound Screening

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Human Immune Cell Assays

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Animal Surgery

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RNA Extraction and Purification

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RNA extraction from a wide variety of sample formats

RNA extraction from a wide variety of sample formats

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In vivo PK/PD Studies

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A large animal model of neuropathic and nociceptive pain for preclinical evaluation of analgesic or neuro-protective therapies. The sciatica model was developed in the pig, providing more clinically relevant data at the preclinical stages. The model is suitable for evaluating both efficacy and PK/PD in the same animal and can be... Show more »

A large animal model of neuropathic and nociceptive pain for preclinical evaluation of analgesic or neuro-protective therapies. The sciatica model was developed in the pig, providing more clinically relevant data at the preclinical stages. The model is suitable for evaluating both efficacy and PK/PD in the same animal and can be used for systemic, local and cell therapies as well as slow release drugs and devices.

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IBS/IBD Animal Models

Irritable Bowel Syndrome/Disease Animal Models
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DSS and TNBS-induced; custom protocols available upon request, as are derivative model validations

DSS and TNBS-induced; custom protocols available upon request, as are derivative model validations

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Brown Rat (Rattus norvegicus) Mouse Gastroenterology Inflammation Colitis Inflammatory Bowel Disease Ulcerative colitis Crohn's disease DSS- induced colitis TNBS- induced colitis Trinitrobenzenesulfonic acid- induced colitis Dextran Sulfate Sodium- induced colitis Microbiology Show 13 more tags Show less

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Pharmacology

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Within our laboratory services, our pharmacology services include specialized diagnostic testing, clinical trial testing, GLP and GMP assessments of compounds/biologics and more! Our goal is to facilitate the advancement of diagnostic capabilities and improve analytical methods through application of rigorous biomarker-based strategies.

Within our laboratory services, our pharmacology services include specialized diagnostic testing, clinical trial testing, GLP and GMP assessments of compounds/biologics and more! Our goal is to facilitate the advancement of diagnostic capabilities and improve analytical methods through application of rigorous biomarker-based strategies.

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Immunophenotyping

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Cytometry

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Cytometry Services

Cytometry Services

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Flow Cytometry

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Multiparametric (16+ color) on the BD LSR Fortessa instrument

Multiparametric (16+ color) on the BD LSR Fortessa instrument

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BD LSRFortessa Miltenyi MacsQuant Brown Rat (Rattus norvegicus) Human Mouse Pig Dog Cat Nonhuman Primate Immunology Oncology CNS/Neurology Cardiovascular Immuno-Oncology Inflammation Non-Alcoholic Steatohepatitis (NASH) Non-alcoholic fatty liver disease (NAFLD) Liver Disease Autoimmune disease Flow cytometry Immunophenotyping immunology antibody validation Show 23 more tags Show less

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Experimental Autoimmune Encephalomyelitis (EAE) Animal Models

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Variety of highly-validated EAE models with multiple induction methods, customizable protocols and advanced analytical readout capabilities

Variety of highly-validated EAE models with multiple induction methods, customizable protocols and advanced analytical readout capabilities

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Brown Rat (Rattus norvegicus) Mouse Rat Immunology CNS/Neurology Inflammation Multiple sclerosis (MS) Demyelinating diseases MOG- induced Experimental Autoimmune Encephalomyelitis (EAE) MBP-induced Experimental Autoimmune Encephalomyelitis (EAE) PLP-induced Experimental Autoimmune Encephalomyelitis (EAE) Myelin Oligodentrocyte Glycoprotein-Induced EAE Myelin Basic Protein-induced EAE Proteolipid Protein-induced EAE Spinal Cord Homogenate-induced EAE SCH-induced EAE Show 16 more tags Show less

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Ex Vivo Models and Assays

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Ex Vivo Models and Assays Services

Ex Vivo Models and Assays Services

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Cell Signaling Analyses

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Cell Signaling Analyses Services

Cell Signaling Analyses Services

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Cell Viability & Proliferation Assays

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Cell Viability & Proliferation Assays Services

Cell Viability & Proliferation Assays Services

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Cell Invasion & Migration Assays

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Cell Invasion & Migration Assays Services

Cell Invasion & Migration Assays Services

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Transfection

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Transfection Services

Transfection Services

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Mammalian Cell Culture

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Mammalian Cell Culture Services

Mammalian Cell Culture Services

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Bioparticle Technology

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Bioparticle Technology Services

Bioparticle Technology Services

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Animal Anxiety and Depression Tests

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Animal Anxiety and Depression Tests Services

Animal Anxiety and Depression Tests Services

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Animal Motor Function Tests

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Animal Motor Function Tests Services

Animal Motor Function Tests Services

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Drug Absorption Studies

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Drug Absorption Studies Services

Drug Absorption Studies Services

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In vitro ADME/DMPK Studies

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In vitro ADME Services

In vitro ADME Services

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Protein Expression Visualization

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Protein Expression Visualization Services

Protein Expression Visualization Services

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Mammalian Models

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Mammalian Models Services

Mammalian Models Services

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Model Organisms

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Model Organisms Services

Model Organisms Services

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Formulation Services

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Formulation Services

Formulation Services

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Clinical Hematology

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Clinical Hematology Services

Clinical Hematology Services

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Common Histochemical Stains

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Common Histochemical Stains Services

Common Histochemical Stains Services

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Histological Staining

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Histological Staining Services

Histological Staining Services

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Clinical and Anatomic Pathology

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Clinical and Anatomic Pathology Services

Clinical and Anatomic Pathology Services

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Primary Cell Culture

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Primary Cell Culture Services

Primary Cell Culture Services

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Cell and Tissue Culture

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Cell and Tissue Culture Services

Cell and Tissue Culture Services

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Human Primary Cells

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Human Primary Cells Services

Human Primary Cells Services

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Tissue Sectioning

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Tissue Sectioning Services

Tissue Sectioning Services

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Tissue Embedding

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Tissue Embedding Services

Tissue Embedding Services

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Tissue Processing

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Tissue Processing Services

Tissue Processing Services

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Human Whole Blood & PBMCs

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Human Whole Blood & PBMCs Services

Human Whole Blood & PBMCs Services

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