We are proud to announce our new location in Cambridge, MA
MD Biosciences Preclinical Laboratory Services is a niche preclinical research group working with biopharmacuetical and medical device companies. Our core therapeutic areas are oncology, inflammation, CNS, pain and cardiovascular with an emphasis on the interplay between these systems. We place a great deal of importance on the scientific rationale of each individual study to ensure that it meets the goals of the R&D program. We are willing to invest in custom study design, development and validation with our sponsors to achieve clinically relevant data at the preclinical stages. We feel that this will help reduce the failure rate in clincial stages and the burden upon the industry. We employ a highly talented group of scientists consisting of PhD, MSc and Bachelor degrees. Each scientist is specialized and highly skilled in their area of expertise. Our group has all been trained in various techniques including microsurgical procedures, injection methods, model and method development as well as imaging and histological techniques. **Selected Publications** - Castel, D., Willentz, E., Doron, O., Brenner, O. and Meilin, S. (2014), Characterization of a porcine model of post-operative pain. *European Journal of Pain*, 18: 496–505. doi: 10.1002/j.1532-2149.2013.00399.x - Meilin, S., Machicao, F., & Elmlinger, M. (2014). Treatment with Actovegin improves spatial learning and memory in rats following transient forebrain ischaemia. *Journal of Cellular and Molecular Medicine* (8), 1623–1630. doi:10.1111/jcmm.12297
Offering comprehensive biostatistical consulting services including analysis and review from study design to study readouts to optimize research needs.
Neurodegenerative diseases like Parkinson's as well as neuro-inflammatory diseases such as multiple sclerosis (MS), with additional capabilities in stroke and cerebral ischemia. Offering customizable study options upon request.
the left sciatic nerve is ligated causing inflammation and swelling, and ultimately peripheral pain.
Dantec KEYPOINT Focus tool utilized to measure electrophysiological action potentials. Capabilities in the assessment of muscle, motor and sensory action potential in rodent models of peripheral nerve injury to measure nerve regeneration, degeneration and protection in response to various treatments. Can apply to non-rodent species and pig models.
A behavioral test used on rats to assess responses to threat of drowning
A test measuring anxiety in laboratory animals that usually uses rodents as a screening test
Asymmetry analysis assesses behavioral deficits in rats after stroke or brain injury.
Large animal models offer high translatability from preclinical research to clinical trials. We offer pig models in neuropathic pain, post-operative pain, wound healing and other dermatology studies and pig nerve block.
MD Biosciences offers models to study the inflammatory components involved in lung inflammation and asthma including OVA-induced asthma, HDM-induced asthma, LPS-acute lung injury, and bleomycin-induced fibrosis.
in vitro, in vivo and ex vivo modeling services for efficacy determinations, PK/PD assessments, mechanism of action studies, compound screening, biomarker analysis and more!
MD Biosciences features a wide range of validated in vivo models of human disease to support preclinical drug efficacy tests, and we provide customizable disease-specific models in order to fit each study's needs.
Variety of preclinical animal models for pharmacology, efficacy and mechanism of action studies in addition to biomarker analysis
Variety of methods across all major platforms for biomarker identification, development, analysis, qualification, validation and downstream application
Our capabilities include support for projects at every stage in the development pipeline, from the initial biomarker identification, qualification and validation through to downstream clinical applications. Some services include identification of druggable targets, characterization of indicators of disease severity or stratification of patient populations.
experimental autoimmune encephalomyelitis (EAE) and MS, EAE has been used as a model for the study of human demyelinating disease.
plate-based assay technique designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones
offers commonly used models of acute inflammatory pain such as CFA and carrageenan - that can be extended - to evaluate a more chronic inflammatory pain state. We can help you study the release of Cytokines and their role in the initiation and maintenance of the inflammatory response along with prostaglandin
Monosodium iodoacetate (MIA) induced osteoarthritis in rats. Cartilage damage and degradation leads to a chronic neuronal damage with neuropathic characteristics.
Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, and passive cutaneous anaphylaxis are our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry.
MD Biosciences offers a wide range of pre-clinical pain models for nociception that can be customized according to sponsors criteria. Validated models include tail flick, visceral and capsaicin.
Focal ischemia (MCAo) models are commonly used to mimic the human stroke situation, however the global ischemia model (4VO) would be highly relevant in clinical conditions such as cardiac arrest.
The non-obese diabetic (NOD) mouse model develops spontaneous autoimmune diabetes, which shares similarities with type 1 diabetes. The STZ-induced diabetic model involves an autoimmune process that results in the destruction of Langerhans islets.
Passive Cutaneous Anaphylaxis (PCA) is an immediate dermal response to an allergan-IgE interaction. It is based on a passively transferred anaphylactic reaction from antiserum. Assessments include dry extravasation (mm), absorbancy, PK blood analysis and biomarker analysis.
Delayed Type Hypersensitivity (DTH) model evaluates the cell-mediated immune response to repeated exposure of skin to oxazolone. This results in a DTH reaction involving CD4+, CD8+ T cells and macrophages, resulting in local skin inflammation characterized by an increased thickness of the skin.
FITC & DNCB-induced Contact Dermatitis Models. These models share many of the features of human contact dermatitis, both sensitization and challenge occurs epicutaneously.
IMQ-Induced Psoriasis Assay
This rapid model of psoriasis-like inflammation involves the IL-17/IL-23 axis. Imiquimod is a potent TLR7/8 ligand and potent immune modulator. Application of imiquimod on the back and ear of mice induces a psoriasis-like inflammation.
Following induction by the intranasal administration of LPS, leukocytes (mainly neutrophils) are recruited to the lung. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.
Acute DSS-induced colitis in mice and rats
Chronic DSS-induced colitis in mice
TNBS-induced colitis in mice and rats
Collagen induced arthritis (CIA) and adjuvant induced arthritis (AIA) are two commonly used models for this purpose. At MD Biosciences we offer our clients the novel and rapid collagen antibody-induced arthritis (CAIA) model as well.
The Global Ischemia model involves permanent occlusion of vertebral arteries and transient occlusion of carotid arteries. The 4VO model can be used to evaluate not only lack of blood flow to brain as found in cardiac arrest and coronary bypass surgery, but also neurodegeneration, dementia and oxygen stress.
LPS is used to induce sepsis in mice, setting off an immune response activating immune cells that excrete cytokines.
The focal cerebral ischemic model is commonly used to assess ischemic injury in the brain, evaluate stroke or neurodegeneration as a result of oxygen stress.
Validated POP and Brennan models using pigs and mice. Pigs demonstrate notable resemblance to human anatomy & physiology. This includes analogous dermal epidermal thickness, dermal papillary bodies and abundant subdermal adipose tissue to humans. Functionally, pig and human are similar in epidermal turnover time, type of keratinous proteins and lipid composition of the stratum corneum.
The murine OVA-induced asthma model is a widely used model that results in the characteristic features of asthma allowing the study and assessment of novel treatments.
he House Dust Mite (HDM) murine model of allergic asthma is used to assess therapies with potential indications in airway inflammation and/or pulmonary inflammation driven primarily by repeated exposure to allergens. Model includes Th2- associated cytokine response, eosinophilic infiltration into the lungs, airway hyper responsiveness (AHR) and epithelial tissue remodeling.
MD Biosciences offers a wide range of pre-clinical pain models for nociception that can be customized according to sponsors criteria.
The rapid preclinical model of sciatic nerve block enables researchers to directly access and dose to the sciatic nerve and allows the screening of new nerve blocking agents or drugs designed to reverse the local analgesia. The capability can be applied to other models such as the SNL and CCI models.
Models in both the rodent as well as the pig bringing much needed translational value from preclinical studies to the human situation.
We conduct preclinical neuropathy models induced with Vincristine or Taxol to mimic the peripheral neuropathy that often results from chemotherapy treatments.
MD Biosciences has validated ischemia-induced renal injury models (unilateral and bilateral) and has familiarity with a variety of other methods should Sponsors have interest in performing validation work on a cost-share basis.
We offer the classic 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)- and 6-hydroxydopamine (6-OHDA)-induced neurotoxin models and genetic models of the disease.
MDB offers a validated neurite outgrowth assay provides an in vitro method to examine the potential effects of exogenous compounds on neuronal activity and development, providing valuable information on the potential of therapeutic compounds to either enhance or inhibit neuritogenesis.
The LPS lung inflammation model is an acute lung inflammation model where researchers can observe the pulmonary response following exposure to bacteria. Using this model, it is possible to measure and characterize the cellular profile of recruited leukocytes to the lung airways as well as the levels of pro-inflammatory cytokines.
MD Biosciences offers various models of wound healing in both rodents and pigs. While rodent models provide data for earlier research stages, pig models are ideally suited for wound healing efficacy studies.
We conduct metabolic and inflammation research, with diet-induced rat and mouse models, and including the following assessments: feed intake, body weight, insulin levels, glucose tolerance, blood pressure, feed preparing, and biomarkers.
Metabolic disease services include models for diabetes, renal, obesity and inflammatory bowel disease. Multiple induction methods may be available for each disease. Diabetes models may be induced with streptozotocin, the use of db/db or NOD mice. Inflammatory Bowel Disease (IBD) may be induced with TNBS or DSS.
MD Biosciences is proud to offer comprehensive diagnostic services for sample analysis and assay development performed to CLIA and GLP regulatory compliance. We provide a wide range of services for specialized diagnostic testing, clinical trial testing, GLP and GMP assessments of compounds/biologics and more! Our goal is to facilitate the advancement of diagnostic capabilities and improve analytical methods through application of rigorous biomarker-based strategies.
Bioanaylsis for all sample types across all major methodologies.
Ovalbumin-induced allergic asthma is a widely used model to reproduce the airway eosinophilia, pulmonary inflammation and elevated IgE levels found during asthma.
The House Dust Mite (HDM) model demonstrates significant relevance for investigating the molecular mechanisms of allergic asthma pathogenesis.
LPS lung inflammation models are widely used to study host-response patterns in the pulmonary compartment.
Bleomycin induced lung injury model in mice is used for studying idiopathic pulmonary fibrosis (IPF).
Screening and mechanism of action studies using primary or immortalized cell lines. Our scientists development experience crosses a broad range of study targets. Customizing each study for sponsor relevance including chosen cell system, stimulant time point(s), and read-outs.
Offering validated models for neurodegenerative diseases including Parkinson's and neuro-inflammatory diseases such as multiple sclerosis (MS) with additional capabilities in stroke and cerebral ischemia. Our laboratories are also proud to be known as a center of excellence in the study of neuropathic, chronic and acute pain, offering multiple validated models and relevant pain read-outs. Our pain services are extended with unique pig translational models complementing our rodent studies and offering confidence as compounds move to IND and clinical stages of their development.
Dermal research with a host of preclinical and translational models. Imiquimod and IL23-induced psoriasis, FITC or DNCB-induced dermatitis, oxazolone-induced delayed-type hypersensitivity, as well as passive cutaneous anaphylaxis form the stable of our validated model offerings. Models are supported by a range of read-outs including protein and mRNA, histology and flow cytometry
Our highly-experienced team of biomarker experts applies systematic, scientifically-rigorous approaches to the discovery and development of biomarker candidates to provide solutions to complex translational questions.
We offer validated models of many inflammatory diseases including skin (IL-23 psoriasis, dermatitis, delayed type hyper-sensitivity, anaphylaxis), gastro-intestinal (TNBS or DSS induced IBD), respiratory (acute lung injury, asthma, fibrosis), joint (rheumatoid arthritis) and sepsis (LPS induced)
Nucleic acid extractions from multiple sample types (fresh, frozen & FFPE tissues, liquid biopsies, disaggregated tumor samples, etc)
Assays include human primary cells, immortalized cell lines, murine and rat primary cells, ex vivo primary cells and custom assays.
The non-obese diabetic (NOD) mouse model develops spontaneous autoimmune diabetes, similar to type 1 diabetes. The STZ-induced diabetic model involves an autoimmune process that results in the destruction of Langerhans islets. Multiple assessments available, and models available are rat, mouse and pig.
Various cancer models and specializes in murine solid tumors for both subcutaneous and orthotopic models, with an emphasis on disease progression, biomarkers and immunophenotyping. Other models include metastatic, xenographic and syngeneic.
Bioanaylsis for all sample types across all major methodologies.
A commonly used model to test the effect of new analgesic drugs in post-operative (incisional) pain is the Brennan model. This model can provide good answers to systemic drugs it is less suitable for testing local treatments such as implants, patches, medical devices and creams.
Clinical urine tests are various tests of urine for diagnostic purposes offered by our clinical services.
RNA extraction from a wide variety of sample formats
A large animal model of neuropathic and nociceptive pain for preclinical evaluation of analgesic or neuro-protective therapies. The sciatica model was developed in the pig, providing more clinically relevant data at the preclinical stages. The model is suitable for evaluating both efficacy and PK/PD in the same animal and can be used for systemic, local and cell therapies as well as slow release drugs and devices.
DSS and TNBS-induced; custom protocols available upon request, as are derivative model validations
Within our laboratory services, our pharmacology services include specialized diagnostic testing, clinical trial testing, GLP and GMP assessments of compounds/biologics and more! Our goal is to facilitate the advancement of diagnostic capabilities and improve analytical methods through application of rigorous biomarker-based strategies.
Multiparametric (16+ color) on the BD LSR Fortessa instrument
Variety of highly-validated EAE models with multiple induction methods, customizable protocols and advanced analytical readout capabilities
Cell Viability & Proliferation Assays Services
Cell Invasion & Migration Assays Services
Animal Anxiety and Depression Tests Services
Protein Expression Visualization Services
Clinical and Anatomic Pathology Services
Biostatistics & Bioinformatics Services
"MD Biosciences is an experienced lab with considerable knowledge and expertise. I found them very easy to work with. Communication was outstanding and I was very pleased by the quality of the work from protocol construction through in life study execution to the final report. I would absolutely use their services again."
"MD Biosciences worked with our small sample volumes. They provided comprehensive results in a clear and practical format. Highly recommended!"
MD Biosciences Preclinical Laboratory Services has not received any endorsements.