The Massachusetts Host-Microbiome Center core facilities provide support to groups around the world who are studying effects of the microbiota on the host. Core staff and equipment handles materials from in vitro systems, animal models and human studies. The core provides personnel, resources and equipment for
(1) quantiative microbiology and microbiological assessment of complex samples
(2) maintenance of mice under germfree (gnotobiotic) or specifically-colonized conditions
(3) CLAMS metabolic caging units, including capacity to operate under germfree or defined-colonization conditions
(3) Technical sequencing for 16S rRNA gene analyses (phylotyping)
(4) Bacterial whole genome sequencing
(5) Short chain fatty acid (SCFA) and other microbial metabolite analyses in primary gut samples or culture supernatants
(6) CLIA-level (clinical laboratory) testing for clinical microbiology, Clostridium difficile detection (antigen, PCR and cell-culture based assays), fecal calprotectin, and screening of human or animal samples for multi-drug resistant organisms (MDROs).
The core maintains gnotobiotic/germ free isolators for housing of mice under germfree conditions, and biohazard levels 1 and 2 for specifically-associated mice. In-house bred germfree mice are also available to investigators for experiments.
In addition, the core maintains an extensive repository of commensal isolates and BL-2 pathogens for use in specific-associations as well as for in vitro studies. Experienced microbiologists routinely cultures primary samples from humans and/or animals to study complex samples using culture and/or sequencing-based methods. Equipment in the core includes a large 2-person Coy anaerobic hood, specialized incubators for culture of microaerophilic organisms, and equipment for biochemical and molecular typing of primary samples.
CLIA (clinical laboratory) testing services to support Microbiome Trials
The Center's CLIA Unit offers the following clinical laboratory tests:
Animal necropsy/tissue harvest and processing for histology or microbiological assessment, etc.
With commensal or pathogenic species in hand, the Gnotobiotics aspect of the core provides the only germfree facility in the Longwood Medical Area where investigators can specifically-associate germfree mice, or maintain germfree controls, to evaluate metabolic, immunologic and other physiologic effects of microbial colonization of the host.
Massachusetts Host-Microbiome Center has not received any reviews.
Massachusetts Host-Microbiome Center has not received any endorsements.