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Macrogen

ic 10.0/10 Overall Rating 100% Positive
ic 4 Orders Completed
Rockville, Maryland, US

About Macrogen

Founded: 2012 Type: Public Company Size: 11-50 employees

Established in 1997, Macrogen provides an array of genetic technology services including NGS, Capillary sequencing, microarray, oligo synthesis and bioinformatics. Macrogen has provided high quality publication grade data to researchers and clinicians in over 100 countries, with locations in the U.S., Korea,... Show more »

Established in 1997, Macrogen provides an array of genetic technology services including NGS, Capillary sequencing, microarray, oligo synthesis and bioinformatics. Macrogen has provided high quality publication grade data to researchers and clinicians in over 100 countries, with locations in the U.S., Korea, the Netherlands, Japan and South America.

Macrogen has been CLIA certified since 2012. Our laboratory provides CLIA WGS and WES sequencing. We also provide CLIA compliant sequencing.

Our laboratories have sequencing platforms including Hiseq X Ten, HiSeq4000, HiSeq2500, PacBio and Life Technologies Ion Proton, among many others. By adapting to cutting-edge technologies, evolving sequencing platforms and utilizing the knowledge and expertise of our staff, Macrogen provides top quality results with the fastest possible turnaround time to our clients.

We’ve experienced exponential growth of our client bases utilizing both clinical and research data. Some of our more notable clients include researchers from the National Institute of Health (NIH), the National Cancer Institute (NCI), and the Food and Drug Administration (FDA) who have grown to trust and rely on Macrogen for their everyday sequencing needs. Below is an example of a study using our data, published in Nature.

Park et al. Discovery of common Asian copy number variants using integrated high-resolution array CGH and massively parallel DNA sequencing. Nature Genetics (2010) 42, 400-405 | doi:10.1038/ng.555

http://www.nature.com/ng/journal/v42/n5/abs/ng.555.html

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Certifications & Qualifications

Agilent Service Provider

Our Services (53)


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Whole Genome Sequencing

Starting at $899.00 per sample

Year End Promotion! (In Bold)

Human whole genome sequencing allows for detection of variations to discover potential correlations to
certain disease risks, and it can also play a role as molecular biomarkers for disease diagnosis and
prediction.

Whole Genome Sequencing at $899 per sample! Below are the specifications of... Show more »

Year End Promotion! (In Bold)

Human whole genome sequencing allows for detection of variations to discover potential correlations to
certain disease risks, and it can also play a role as molecular biomarkers for disease diagnosis and
prediction.

Whole Genome Sequencing at $899 per sample! Below are the specifications of the promotion:
-       100Gb output per sample
-       Novaseq6000 S4
-       150bp Paired End sequencing
-       Raw data (fastq) delivery
-       No minimum number of samples
- (However, turnaround time may be affected depending on volume)
- Samples must be received by November 30, 2018

  • De novo sequencing is typically performed without prior knowledge of the sequencing data, De novo
    sequencing has proven successful for confirming and expanding upon results from database searches,
    providing excellent resources for understanding a species.

  • Some of the most crucial information, obtained by resequencing of organism’s genome DNA, are the
    individual variations in the genome, such as single nucleotide polymorphism(SNP), copy number
    variation(CNV), and structural variation

Sequencing platform
- NovaSeq6000
- HiSeq X10
- HiSeq2000 / HiSeq4000 / MiSeq system (Paired-end, Mate pair)

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Illumina HiSeq 2500 Illumina MiSeq Illumina HiSeq X Ten Illumina HiSeq 2000 Human Bacteria Non-human Show 7 more tags Show less

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Whole Exome Sequencing (WES)

Starting at $229.00 per sample

Year End Promotion!
100X raw coverage (5-6 Gb per Kit)
- Agilent SureSelect V5/V6/V7 Kit
- NovaSeq6000 150bp PE
- Raw data (Fastq) delivery via FTP
- TAT: 4-6 weeks
- Minimum sample requirement: 20
- Samples must be received by 11/30/2018

We require 1ug of gDNA for Agilent SureSelect Human All Exon V4 (51M) or V5... Show more »

Year End Promotion!
100X raw coverage (5-6 Gb per Kit)
- Agilent SureSelect V5/V6/V7 Kit
- NovaSeq6000 150bp PE
- Raw data (Fastq) delivery via FTP
- TAT: 4-6 weeks
- Minimum sample requirement: 20
- Samples must be received by 11/30/2018

We require 1ug of gDNA for Agilent SureSelect Human All Exon V4 (51M) or V5 (50M) capture kit, sequencing will be performed on the latest platform, Illumina HiSeq 4000 with 100X RAW DATA.

CLIA Level Sequencing

In the U.S., the Centers for Medicare & Medicaid Services (CMS) regulates all laboratory testing (except research) performed on humans through the Clinical Laboratory Improvement Amendments (CLIA). The objective of the CLIA program is to ensure quality laboratory testing. Macrogen Clinical Laboratory has received CLIA certification from U.S. CMS and provides CLIA quality of Whole Exome Sequencing.
Macrogen Clinical Laboratory performs routine quality management procedures including laboratory condition and validation of equipment and instrument to guarantee high quality of data. Macrogen Clinical Laboratory also performs QC (quality control) with higher standard and all laboratory procedures are processed by certified personnel only.

Safety

  • Restricted laboratory access
  • CLIA level technical processes
  • Secure Protected Data
  • Secured and on-site server analysis

Enhanced Analysis

  • Customized analysis for various hereditary diseases and cancers
  • Project consultation about clinical genomic research
  • Clinical service advice
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Illumina HiSeq 2500 Illumina HiSeq 4000 Illumina HiSeq 2000 Human Agilent SureSelect XT Human All Exon v5 Agilent SureSelect XT Human All Exon v4 Show 6 more tags Show less

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RNA Sequencing

Price on request
Request a quote for more information about this service.
Illumina HiSeq 2500 Illumina HiSeq 2000 Human Bacteria Non-human

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Small RNA Sequencing

Price on request

Small RNA molecules are encoded from plant and animal genomes, and they are responsible for regulating gene expression.

Macrogen's NGS technology is able to sequence and quantify all the small RNA families in a sample and profile the miRNA, siRNA, piRNA and other non-coding RNAs.

Available Platforms

Small RNA molecules are encoded from plant and animal genomes, and they are responsible for regulating gene expression.

Macrogen's NGS technology is able to sequence and quantify all the small RNA families in a sample and profile the miRNA, siRNA, piRNA and other non-coding RNAs.

Available Platforms

  • Illumina HiSeq2000 / HiSeq2500 / MiSeq

Data Analysis

  • Expression Profile
  • Novel small RNA
  • Differentially Expressed miRNA
  • Target Prediction of Known and Novel miRNA
  • Gene Set Analysis
  • Comparative Analysis
  • Customized Analysis
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Illumina HiSeq 2500 Illumina MiSeq

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18S rRNA Sequencing

Price on request

Analysis on sequences and confirmation of microorganism's homogenic data using rRNA database (NCBI) after
amplification of Bacteria or Fungi's RNA ribosomal.

All processes including Bacteria/Fungi gDNA extraction, PCR amplification, sequencing, and assembly.

FUNGI
1. Analysis on 18S rRNA region sequences; length greater... Show more »

Analysis on sequences and confirmation of microorganism's homogenic data using rRNA database (NCBI) after
amplification of Bacteria or Fungi's RNA ribosomal.

All processes including Bacteria/Fungi gDNA extraction, PCR amplification, sequencing, and assembly.

FUNGI
1. Analysis on 18S rRNA region sequences; length greater than 1,600 bp guaranteed.
2. Analysis on ITS region (18S prior to update); length greater than 500 bp guaranteed.
3. Analysis on 26S rRNA gene(D1/D2/D3 region) length greater than 1,300 bp guaranteed.

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Fungi

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Epigenomic Sequencing

Price on request
  • MBD(Methyl-CpG binding domain)
  • Chip(Chromatin Immuno-precipitation)

Methylation is one of the major pathways of gene expression regulation in chromosomal DNA. Therefore, to characterize methylation of bases is to understand the regulation of genes. Comparison of the sequence obtained from the bisulfite-treated amplicon to... Show more »

  • MBD(Methyl-CpG binding domain)
  • Chip(Chromatin Immuno-precipitation)

Methylation is one of the major pathways of gene expression regulation in chromosomal DNA. Therefore, to characterize methylation of bases is to understand the regulation of genes. Comparison of the sequence obtained from the bisulfite-treated amplicon to the published sequence enables the identification of differential methylations. The methylation state on the target regions, (e.g. CpG islands) can be quantitatively characterized.

Sequencing platform

HiSeq2000 / MiSeq system (Methylation/ MBD, Chip sequencing )

Data Analysis

  • Standard data Analysis
  • Global Methylation Profiles
  • Specific Methylation Profiles
  • CG Islands / Differentially Methylation Regions(DMRs)
  • Advanced data analysis
  • DMR-Associated Genes
  • Gene Set Analysis
  • Comparative Analysis
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Targeted Sequencing

Price on request
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Sanger Sequencing

Price on request
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Targeted Exome Sequencing

Price on request

Macrogen offers a complete solution for variant discovery using exome sequencing with next-generation sequencing platforms. Macrogen offers extensive data filtering and mapping for accurate SNP and InDel determina-tions. Custom data analysis is available through the Macrogen Clinical Laboratory Turn-key Discovery Services to... Show more »

Macrogen offers a complete solution for variant discovery using exome sequencing with next-generation sequencing platforms. Macrogen offers extensive data filtering and mapping for accurate SNP and InDel determina-tions. Custom data analysis is available through the Macrogen Clinical Laboratory Turn-key Discovery Services to provide clients an affordable option to rapidly identify putative variant candidates.

Enrichment methods

  • Illumina TruSeq Exome Capture
  • Agilent SureSelect Exome Capture
  • NimbleGen SeqCap EZ Capture

Sequencing platforms

  • Latest Ilumina Hiseq 2000/2500 system with
  • 100 bp paired-end sequencing

Sequencing coverage

  • Sequence to 50X or 100X coverage at the raw data level
  • On-target coverage of 25X, 30X, 50X or higher
  • Custom design on-target coverage levels
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Illumina HiSeq 2500 Illumina MiSeq Illumina HiSeq 2000 Ion Torrent Ion Proton Ion Torrent Personal Genome Machine (PGM) Mouse Cow Agilent SureSelect Show 8 more tags Show less

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SMRT Sequencing

Single Molecule Real-Time Sequencing
Price on request

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen... Show more »

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen sequencing: the use of various Next-Gen systems to take advantage of the new technology and the combination of different bioinformatics tools and their stepwise application.We provide all kinds of NGS:

  • Whole genome resequencing/ De novo
  • Whole Exome sequencing, Targeted sequencing
  • Transcriptome resequencing/ De novo (including small RNA)
  • Metagenome 16s/ shotgun sequencing
  • ChiP sequencing
  • Amplicon sequencing
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PacBio RS II

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mRNA Sequencing

Starting at $169.00 per sample

mRNA Sequencing - Promo
Year End Promotion!
40M total reads (20M read pairs)
- TruSeq stranded mRNA kit (poly A)
- NovaSeq6000 150bp PE
- Raw data (Fastq) delivery via FTP
- TAT: 4-6 weeks
- Minimum sample requirement: 16
- Samples must be received by 11/30/2018.

With the advent of RNA sequencing... Show more »

mRNA Sequencing - Promo
Year End Promotion!
40M total reads (20M read pairs)
- TruSeq stranded mRNA kit (poly A)
- NovaSeq6000 150bp PE
- Raw data (Fastq) delivery via FTP
- TAT: 4-6 weeks
- Minimum sample requirement: 16
- Samples must be received by 11/30/2018.

With the advent of RNA sequencing technologies, the profiling and characterization of transcriptome is now possible and affordable. With transcriptome sequencing, researchers can study a variety of transcriptional activities.

Macrogen offers a complete solution for transcriptome sequencing applications using either the Illumina HiSeq 2500 system or NovaSeq 6000 system.

Compared to traditional array-based gene expression methods, mRNA sequencing provides more extensive information about isoforms, splice variants and fusion genes. These advantages allow for a more comprehensive view of the underlying biology at the RNA level.

Available Platforms

  • Illumina NovaSeq 6000 / HiSeq2500 / MiSeq

Data Analysis (de novo)

  • De novo Assembly
  • Expression Profile
  • Gene Annotation
  • Gene Set Analysis
  • Variant Calling (SNP, In/Del) and Annotation
  • Customized Analysis

Data Analysis (re-sequencing)

  • Expression Profile
  • DEG, Fusion Gene (Human/Animal/Plant)
  • Variant Calling & Annotation (Human/Animal/Plant)
  • Gene Ontology, Gene Set Analysis (Microbe)
  • Customized Analysis
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Illumina HiSeq 2500 Illumina MiSeq Illumina HiSeq 2000 Roche GS-FLX 454 Roche GS-FLX+

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Next Generation Sequencing (NGS)

Price on request

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen... Show more »

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen sequencing: the use of various Next-Gen systems to take advantage of the new technology and the combination of different bioinformatics tools and their stepwise application.We provide all kinds of NGS:
•Whole genome resequencing/ De novo

•Whole Exome sequencing, Targeted sequencing

•Transcriptome resequencing/ De novo (including small RNA)

•Metagenome 16s/ shotgun sequencing

•ChiP sequencing

•Amplicon sequencing
Available Platforms:
-SMRT seq
-Roche 454 NGS
-SOLiD NGS
-Illumina NGS
-Ion Torrent NGS

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Illumina HiSeq 2500 Illumina MiSeq Illumina HiSeq X Ten Illumina HiSeq 4000 Illumina HiSeq 2000 Roche GS-FLX 454 Titanium PacBio RS II Roche GS-FLX 454 Roche GS-FLX+ Ion Torrent Ion Proton Ion Torrent Personal Genome Machine (PGM) Applied Biosystems SOLiD III Show 12 more tags Show less

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Metagenomics

Price on request

Metagenome is the whole genetic material present in a given environment, such as a spot of soil, microenvironments within the human body, or a layer of ocean. Metagenome sequencing provides a comprehensive view of the microorganism diversity, as well as profiles of environmental habitats.
Macrogen's faster and cheaper NGS... Show more »

Metagenome is the whole genetic material present in a given environment, such as a spot of soil, microenvironments within the human body, or a layer of ocean. Metagenome sequencing provides a comprehensive view of the microorganism diversity, as well as profiles of environmental habitats.
Macrogen's faster and cheaper NGS sequencing technologies and the ability to sequence uncultured microbes sampled directly from their habitats will expand and transform your view of the microbial world.

Small RNA molecules are encoded from plant and animal genomes, and they are responsible for
regulating gene expression.

Macrogen's NGS technology is able to sequence and quantify all the small RNA families in a sample and profile
the miRNA, siRNA, piRNA and other non-coding RNAs.

Available Platforms
- Illumina HiSeq2000 & 2500 / MiSeq
- 454 GS-FLX Titanium

Data Analysis (Amplicon Method)

  • OTU counting
  • OTU diversity
  • Comparative Analysis

Data Analysis (shotgun Method)
- De novo assembly
- BlastX
- Gene Prediction
- OTU counting (16S rDNA or other housekeeping genes)
- Function assignment to each gene candidate
- Constructing metabolic pathways
- Statistical analysis of two or more meta-genomic samples

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Illumina MiSeq Roche GS-FLX 454 Titanium Bacteria

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Bioinformatics

Price on request

Bioinformatics combines computer science, statistics, mathematics and engineering to study and process biological data.

At Macrogen, highly experienced Bioinformatics specialists are dedicated to offering Bioinformatics services with the very best quality and accuracy. Our analytical capabilities using extensive NGS data... Show more »

Bioinformatics combines computer science, statistics, mathematics and engineering to study and process biological data.

At Macrogen, highly experienced Bioinformatics specialists are dedicated to offering Bioinformatics services with the very best quality and accuracy. Our analytical capabilities using extensive NGS data include but are not limited to

  • Highest quality control and assurances for data and statistics
  • Discovery of SNPs, InDels, and structural variations including CNVs
  • Annotation of variants
  • Association analysis
  • Gene expression analysis
  • Alternative splicing transcripts identification
  • Peak analysis (ChiP-Seq)

All of our processes are assured to bring you accurate data in a timely manner, at a competitive cost with flexible and customizable workflow. More information regarding our processes to Bioinformatics services can be obtained by contacting us directly and speaking with a representative.

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Breast Cancer Gene Panel

Price on request

Macrogen offers diagnostic testing for major cancers
Macrogen is a CLIA licensed laboratory

Benefits
•We can get the most accurate diagnosis through Next Generation Sequencing.

•We can provide the most convincing data because we are CLIA licensed.

•We use ACMG(American College of Medical Genetics and Genomics)... Show more »

Macrogen offers diagnostic testing for major cancers
Macrogen is a CLIA licensed laboratory

Benefits
•We can get the most accurate diagnosis through Next Generation Sequencing.

•We can provide the most convincing data because we are CLIA licensed.

•We use ACMG(American College of Medical Genetics and Genomics) recommended genes to offer patients more credible data.

• We cover most major cancers (Breast Cancer, Pancreatic Cancer, Colon Cancer, Ovarian Cancer, Uterine Cancer, Renal Cancer, Endocrine Cancer, Kidney Cancer, Parathyroid Cancer, Brain Cancer and Eye Cancer)

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Illumina Sequencing

Price on request

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen... Show more »

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen sequencing: the use of various Next-Gen systems to take advantage of the new technology and the combination of different bioinformatics tools and their stepwise application.We provide all kinds of NGS:

  • Whole genome resequencing/ De novo
  • Whole Exome sequencing, Targeted sequencing
  • Transcriptome resequencing/ De novo (including small RNA)
  • Metagenome 16s/ shotgun sequencing
  • ChiP sequencing
  • Amplicon sequencing
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Comprehensive Cancer Gene Panel

Price on request

Macrogen offers diagnostic testing for major cancers
Macrogen is a CLIA licensed laboratory

Benefits

  • We can get the most accurate diagnosis through Next Generation Sequencing.
  • We can provide the most convincing data because we are CLIA licensed.
  • We use ACMG(American College of Medical Genetics and Genomics)... Show more »

Macrogen offers diagnostic testing for major cancers
Macrogen is a CLIA licensed laboratory

Benefits

  • We can get the most accurate diagnosis through Next Generation Sequencing.
  • We can provide the most convincing data because we are CLIA licensed.
  • We use ACMG(American College of Medical Genetics and Genomics) recommended genes to offer patients more credible data.
  • We cover most major cancers (Breast Cancer, Pancreatic Cancer, Colon Cancer, Ovarian Cancer, Uterine Cancer, Renal Cancer, Endocrine Cancer, Kidney Cancer, Parathyroid Cancer, Brain Cancer and Eye Cancer)
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Inherited Disease Gene Panel

Price on request

Macrogen offers diagnostic testing for Hereditary diseases
Macrogen is a CLIA licensed laboratory

Benefits

  • We can get the most accurate diagnosis through Next Generation Sequencing.
  • We can provide the most conclusive data because we are CLIA licensed.
  • We use ACMG(American College of Medical Genetics and... Show more »

Macrogen offers diagnostic testing for Hereditary diseases
Macrogen is a CLIA licensed laboratory

Benefits

  • We can get the most accurate diagnosis through Next Generation Sequencing.
  • We can provide the most conclusive data because we are CLIA licensed.
  • We use ACMG(American College of Medical Genetics and Genomics) recommended genes to offer patients more credible data.
  • We can get easier and more accurate result from diagnostic test that are difficult to diagnose
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Ion Torrent Sequencing

Ion Torrent Next Generation Sequencing
Price on request

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen... Show more »

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen sequencing: the use of various Next-Gen systems to take advantage of the new technology and the combination of different bioinformatics tools and their stepwise application.We provide all kinds of NGS:

  • Whole genome resequencing/ De novo
  • Whole Exome sequencing, Targeted sequencing
  • Transcriptome resequencing/ De novo (including small RNA)
  • Metagenome 16s/ shotgun sequencing
  • ChiP sequencing
  • Amplicon sequencing
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Epigenetic Studies

Price on request
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Clinical Chemistry

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DNA Sequencing

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RNA Services

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Clinical Research

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Pharmacology & Toxicology

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Exome Sequencing Services

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Clinical Laboratory Services

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Computational Modeling

Price on request
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Nucleic Acid Services

Price on request
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Biostatistics & Bioinformatics

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Methyl Binding Domain (MBD)-Enriched Genome Sequencing

Price on request
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De Novo Transcriptome Sequencing

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Metagenomic Sequencing

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De Novo Genome Sequencing

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Gene Panel Testing

Price on request
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Shotgun DNA Sequencing

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Deep Sequencing

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DNA Library Screening

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Biochemistry & Molecular Biology

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Genotyping Services

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DNA Methylation Analysis

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Gene Expression Analysis

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Cloning Vectors

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DNA Amplification

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DNA Libraries

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Antibody/Antigen Detection Based Testing

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Bioanalysis

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Targeted Gene Sequencing

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Biomarkers

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DNA Services

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Biology

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Clinical Molecular Diagnostics

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16S rRNA Sequencing

Starting at $69.00 per sample

16S rRNA Sequencing -100K reads Amplicon Sequencing (promo)

Analysis on sequences and confirmation of microorganism's homogenic data using rRNA database (NCBI) after
amplification of Bacteria or Fungi's RNA ribosomal.

All processes including Bacteria/Fungi gDNA extraction, PCR amplification, sequencing, and... Show more »

16S rRNA Sequencing -100K reads Amplicon Sequencing (promo)

Analysis on sequences and confirmation of microorganism's homogenic data using rRNA database (NCBI) after
amplification of Bacteria or Fungi's RNA ribosomal.

All processes including Bacteria/Fungi gDNA extraction, PCR amplification, sequencing, and assembly.

PCR on 16S rRNA using 27F and 1492R primers; and yielding of 1,300bp or more sequencing data using internal primers 785F, 907R. Basic processing will involve usage two primers 785F and 907R. Client will be allowed to select different additional primers for more sequencing reactions.

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Bacteria

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  • Positive review for 16S rRNA Sequencing:

    June 2018

    "This was a fast and a professional service. I really liked the approach Macrogen arranges my orders in their website, it's easy to handle and to download the files for analysis."

Macrogen has not received any endorsements.