Macrogen

We require 1ug of gDNA for Agilent SureSelect Human All Exon V4 (51M) or V5 (50M) capture kit, sequencing will be performed on the latest platform, Illumina HiSeq 4000 with 100X RAW DATA.

CLIA Level Sequencing

In the U.S., the Centers for Medicare & Medicaid Services (CMS) regulates all laboratory testing (except research) performed on humans through the Clinical Laboratory Improvement Amendments (CLIA). The objective of the CLIA program is to ensure quality laboratory testing. Macrogen Clinical Laboratory has received CLIA certification from U.S. CMS and provides CLIA quality of Whole Exome Sequencing.
Macrogen Clinical Laboratory performs routine quality management procedures including laboratory condition and validation of equipment and instrument to guarantee high quality of data. Macrogen Clinical Laboratory also performs QC (quality control) with higher standard and all laboratory procedures are processed by certified personnel only.

Safety

• Restricted laboratory access
  
• CLIA level technical processes
  
• Secure Protected Data
  
• Secured and on-site server analysis
  

Enhanced Analysis

• Customized analysis for various hereditary diseases and cancers
  
• Project consultation about clinical genomic research
  
• Clinical service advice
  

With the advent of RNA sequencing technologies, the profiling and characterization of transcriptome is now possible and affordable. With transcriptome sequencing, researchers can study a variety of transcriptional activities.

Macrogen offers a complete solution for transcriptome sequencing applications using either the Illumina HiSeq 2000 system or Roche GS-FLX system.

Compared to traditional array-based gene expression methods, mRNA sequencing provides more extensive information about isoforms, splice variants and fusion genes. These advantages allow for a more comprehensive view of the underlying biology at the RNA level.

Available Platforms

• Illumina HiSeq2000 / HiSeq2500 / MiSeq
  
• 454 GS-FLX / Plus
  

Data Analysis (de novo)

• De novo Assembly
  
• Expression Profile
  
• Gene Annotation
  
• Gene Set Analysis
  
• Variant Calling (SNP, In/Del) and Annotation
  
• Customized Analysis
  

Data Analysis (re-sequencing)

• Expression Profile
  
• DEG, Fusion Gene (Human/Animal/Plant)
  
• Variant Calling & Annotation (Human/Animal/Plant)
  
• Gene Ontology, Gene Set Analysis (Microbe)
  
• Customized Analysis

Small RNA molecules are encoded from plant and animal genomes, and they are responsible for regulating gene expression.

Macrogen's NGS technology is able to sequence and quantify all the small RNA families in a sample and profile the miRNA, siRNA, piRNA and other non-coding RNAs.

Available Platforms

• Illumina HiSeq2000 / HiSeq2500 / MiSeq
  

Data Analysis

• Expression Profile
  
• Novel small RNA
  
• Differentially Expressed miRNA
  
• Target Prediction of Known and Novel miRNA
  
• Gene Set Analysis
  
• Comparative Analysis
  
• Customized Analysis

Analysis on sequences and confirmation of microorganism's homogenic data using rRNA database (NCBI) after
amplification of Bacteria or Fungi's RNA ribosomal.

All processes including Bacteria/Fungi gDNA extraction, PCR amplification, sequencing, and assembly.

PCR on 16S rRNA using 27F and 1492R primers; and yielding of 1,300bp or more sequencing data using internal primers 785F, 907R. Basic processing will involve usage two primers 785F and 907R. Client will be allowed to select different additional primers for more sequencing reactions.

Analysis on sequences and confirmation of microorganism's homogenic data using rRNA database (NCBI) after
amplification of Bacteria or Fungi's RNA ribosomal.

All processes including Bacteria/Fungi gDNA extraction, PCR amplification, sequencing, and assembly.

FUNGI
1. Analysis on 18S rRNA region sequences; length greater than 1,600 bp guaranteed.
2. Analysis on ITS region (18S prior to update); length greater than 500 bp guaranteed.
3. Analysis on 26S rRNA gene(D1/D2/D3 region) length greater than 1,300 bp guaranteed.

• MBD(Methyl-CpG binding domain)
  
• Chip(Chromatin Immuno-precipitation)
  

Methylation is one of the major pathways of gene expression regulation in chromosomal DNA. Therefore, to characterize methylation of bases is to understand the regulation of genes. Comparison of the sequence obtained from the bisulfite-treated amplicon to the published sequence enables the identification of differential methylations. The methylation state on the target regions, (e.g. CpG islands) can be quantitatively characterized.

Sequencing platform

HiSeq2000 / MiSeq system (Methylation/ MBD, Chip sequencing )

Data Analysis

• Standard data Analysis
  
• Global Methylation Profiles
  
• Specific Methylation Profiles
  
• CG Islands / Differentially Methylation Regions(DMRs)
  
• Advanced data analysis
  
• DMR-Associated Genes
  
• Gene Set Analysis
  
• Comparative Analysis

Macrogen offers a complete solution for variant discovery using exome sequencing with next-generation sequencing platforms. Macrogen offers extensive data filtering and mapping for accurate SNP and InDel determina-tions. Custom data analysis is available through the Macrogen Clinical Laboratory Turn-key Discovery Services to provide clients an affordable option to rapidly identify putative variant candidates.

Enrichment methods

• Illumina TruSeq Exome Capture
  
• Agilent SureSelect Exome Capture
  
• NimbleGen SeqCap EZ Capture
  

Sequencing platforms

• Latest Ilumina Hiseq 2000/2500 system with
  
• 100 bp paired-end sequencing
  

Sequencing coverage

• Sequence to 50X or 100X coverage at the raw data level
  
• On-target coverage of 25X, 30X, 50X or higher
  
• Custom design on-target coverage levels

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen sequencing: the use of various Next-Gen systems to take advantage of the new technology and the combination of different bioinformatics tools and their stepwise application.We provide all kinds of NGS:

• Whole genome resequencing/ De novo
  
• Whole Exome sequencing, Targeted sequencing
  
• Transcriptome resequencing/ De novo (including small RNA)
  
• Metagenome 16s/ shotgun sequencing
  
• ChiP sequencing
  
• Amplicon sequencing

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen sequencing: the use of various Next-Gen systems to take advantage of the new technology and the combination of different bioinformatics tools and their stepwise application.We provide all kinds of NGS:
•Whole genome resequencing/ De novo

•Whole Exome sequencing, Targeted sequencing

•Transcriptome resequencing/ De novo (including small RNA)

•Metagenome 16s/ shotgun sequencing

•ChiP sequencing

•Amplicon sequencing
Available Platforms:
-SMRT seq
-Roche 454 NGS
-SOLiD NGS
-Illumina NGS
-Ion Torrent NGS

Metagenome is the whole genetic material present in a given environment, such as a spot of soil, microenvironments within the human body, or a layer of ocean. Metagenome sequencing provides a comprehensive view of the microorganism diversity, as well as profiles of environmental habitats.
Macrogen's faster and cheaper NGS sequencing technologies and the ability to sequence uncultured microbes sampled directly from their habitats will expand and transform your view of the microbial world.

Small RNA molecules are encoded from plant and animal genomes, and they are responsible for
regulating gene expression.

Macrogen's NGS technology is able to sequence and quantify all the small RNA families in a sample and profile
the miRNA, siRNA, piRNA and other non-coding RNAs.

Available Platforms
- Illumina HiSeq2000 & 2500 / MiSeq
- 454 GS-FLX Titanium

Data Analysis (Amplicon Method)

• OTU counting
  
• OTU diversity
  
• Comparative Analysis
  

Data Analysis (shotgun Method)
- De novo assembly
- BlastX
- Gene Prediction
- OTU counting (16S rDNA or other housekeeping genes)
- Function assignment to each gene candidate
- Constructing metabolic pathways
- Statistical analysis of two or more meta-genomic samples

Bioinformatics combines computer science, statistics, mathematics and engineering to study and process biological data.

At Macrogen, highly experienced Bioinformatics specialists are dedicated to offering Bioinformatics services with the very best quality and accuracy. Our analytical capabilities using extensive NGS data include but are not limited to

• Highest quality control and assurances for data and statistics
  
• Discovery of SNPs, InDels, and structural variations including CNVs
  
• Annotation of variants
  
• Association analysis
  
• Gene expression analysis
  
• Alternative splicing transcripts identification
  
• Peak analysis (ChiP-Seq)
  

All of our processes are assured to bring you accurate data in a timely manner, at a competitive cost with flexible and customizable workflow. More information regarding our processes to Bioinformatics services can be obtained by contacting us directly and speaking with a representative.

Macrogen offers diagnostic testing for major cancers
Macrogen is a CLIA licensed laboratory

Benefits
•We can get the most accurate diagnosis through Next Generation Sequencing.

•We can provide the most convincing data because we are CLIA licensed.

•We use ACMG(American College of Medical Genetics and Genomics) recommended genes to offer patients more credible data.

• We cover most major cancers (Breast Cancer, Pancreatic Cancer, Colon Cancer, Ovarian Cancer, Uterine Cancer, Renal Cancer, Endocrine Cancer, Kidney Cancer, Parathyroid Cancer, Brain Cancer and Eye Cancer)

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen sequencing: the use of various Next-Gen systems to take advantage of the new technology and the combination of different bioinformatics tools and their stepwise application.We provide all kinds of NGS:

• Whole genome resequencing/ De novo
  
• Whole Exome sequencing, Targeted sequencing
  
• Transcriptome resequencing/ De novo (including small RNA)
  
• Metagenome 16s/ shotgun sequencing
  
• ChiP sequencing
  
• Amplicon sequencing
  

Macrogen offers diagnostic testing for major cancers
Macrogen is a CLIA licensed laboratory

Benefits

• We can get the most accurate diagnosis through Next Generation Sequencing.
  
• We can provide the most convincing data because we are CLIA licensed.
  
• We use ACMG(American College of Medical Genetics and Genomics) recommended genes to offer patients more credible data.
  
• We cover most major cancers (Breast Cancer, Pancreatic Cancer, Colon Cancer, Ovarian Cancer, Uterine Cancer, Renal Cancer, Endocrine Cancer, Kidney Cancer, Parathyroid Cancer, Brain Cancer and Eye Cancer)
  

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen sequencing: the use of various Next-Gen systems to take advantage of the new technology and the combination of different bioinformatics tools and their stepwise application.We provide all kinds of NGS:

• Whole genome resequencing/ De novo
  
• Whole Exome sequencing, Targeted sequencing
  
• Transcriptome resequencing/ De novo (including small RNA)
  
• Metagenome 16s/ shotgun sequencing
  
• ChiP sequencing
  
• Amplicon sequencing
  

Macrogen offers diagnostic testing for Hereditary diseases
Macrogen is a CLIA licensed laboratory

Benefits

• We can get the most accurate diagnosis through Next Generation Sequencing.
  
• We can provide the most conclusive data because we are CLIA licensed.
  
• We use ACMG(American College of Medical Genetics and Genomics) recommended genes to offer patients more credible data.
  
• We can get easier and more accurate result from diagnostic test that are difficult to diagnose
  

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen sequencing: the use of various Next-Gen systems to take advantage of the new technology and the combination of different bioinformatics tools and their stepwise application.We provide all kinds of NGS:

• Whole genome resequencing/ De novo
  
• Whole Exome sequencing, Targeted sequencing
  
• Transcriptome resequencing/ De novo (including small RNA)
  
• Metagenome 16s/ shotgun sequencing
  
• ChiP sequencing
  
• Amplicon sequencing

Next Generation Sequencing(NGS) technologies have become cheaper and easier to use. Meanwhile, large-scale evolutionary studies towards the origin of life for all species and their evolution have become more and more challenging. On the path to the deep understanding of the organism, two parameters are important in the Next-Gen sequencing: the use of various Next-Gen systems to take advantage of the new technology and the combination of different bioinformatics tools and their stepwise application.We provide all kinds of NGS:

• Whole genome resequencing/ De novo
  
• Whole Exome sequencing, Targeted sequencing
  
• Transcriptome resequencing/ De novo (including small RNA)
  
• Metagenome 16s/ shotgun sequencing
  
• ChiP sequencing
  
• Amplicon sequencing