The Loyola Genomics Facility currently provides next-generation sequencing capabilities using the Illumina MiSeq. We are available to guide your next project through all the necessary steps including experimental design, quality control analysis, library preparation, and data analysis. The MiSeq desktop sequencer allows you to access more focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, and HLA typing. The MiSeq is capable of delivering up to 15 Gb of output with 25 million sequencing reads and 2x300 basepair read lengths.
To submit an order please visit the Core Facilities on Loyola Wired and use the Research Channel link.
Additional sequencing or genomics technologies may be acquired depending on the needs, as we continuously evaluate them.
Quality Assurance Solutions:
Quality Control Analysis: | Cost per Sample: |
---|---|
Qubit Fluorometer | $1.00 |
Agilent 2100 Bioanalyzer | $6.00 |
Library Preparation Applications: | Cost per Sample: | Depth of Coverage Required: | Suggested MiSeq Cartridge: |
---|---|---|---|
16S rRNA Gene Amplicon | $16.00 | 20X | MiSeq v2 Reagent Kit (500 cycle) |
Custom Amplicon | $16.00 | 20X | MiSeq v2 Reagent Kit (500 cycle) |
Microbial Whole Genome | $80.00 | 30X | MiSeq v2 Reagent Kit (500 cycle) |
Microbial RNASeq | $315.00 | MiSeq v3 Reagent Kit (300 cycle) | |
Micro RNASeq | $230.00 | MiSeq v2 Reagent Kit (50 cycle) | |
Viral Whole Genome | $105.00 | 10X | MiSeq v2 Reagent Kit (300 cycle) |
Illumina MiSeq Sequencing: | Cost per Run: | Expected Output: |
---|---|---|
MiSeq v2 Reagent Kit (50 cycle) | $1062.00 | 750-850 Mb |
Miseq v2 Reagent Kit (300 cycle) | $1348.50 | 4.5-5.1 Gb |
MiSeq v2 Reagent Kit (500 cycle) | $1512.50 | 7.5-8.5 Gb |
Miseq v3 Reagent Kit (150 cycle) | $1200.00 | 3.3-3.8 Gb |
MiSeq v3 Regent Kit (600 cycle) | $1923.50 | 13.2-15 Gb |
Loyola Genomics Facility has not received any reviews.
Loyola Genomics Facility has not received any endorsements.