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Likarda, LLC

8 Orders Completed
Kansas City, Kansas, US

About Likarda, LLC

Founded: 2012 Size: 1-10 employees

Likarda, LLC is an exciting biotech company bringing to market disruptive technologies that improve healthcare outcomes and lower costs for pets and people alike. Likarda utilizes a proprietary cell culture system to create three-dimensional cell clusters, or miniaturized organs, having applications in markets from drug discovery and development to cell-based transplantation and regenerative medicine. For instance, pharmaceutical companies can test new drug compounds on our human 3D cell clusters, providing a better prediction of how the drugs will work in the body. Likarda’s founders developed its innovations while employed as researchers at the University of Kansas Medical Center where they were focused on discovering and providing next generation treatments for diabetes. The technology consists of a proprietary and patented micromold plate and manufacturing process. In 2012, the founders discovered the power of the Kanslets for secondary drug testing and have multiple publications in support of the technology. Shortly after making this discovery, Likarda was established and the intellectual property and patents were exclusively licensed from the University of Kansas. Likarda partners with its clients to champion high quality, cutting-edge products and services, constantly seeking to create the next improved version. To accomplish this we have well-defined long-range goals, with the added opportunity for flexibility and growth. Founded in 2012 **Publications** - Amin J, Ramachandran K, Williams SJ, Lee A, Novikova L, Stehno-Bittel L. A simple, reliable method for high-throughput screening for diabetes drugs using 3D β-cell spheroids. *J Pharmacol Toxicol Methods*. 2016 Aug 20;82:83-89. doi: 10.1016/j.vascn.2016.08.005. - Ramachandran K, Peng X, Bokvist K, Stehno-Bittel L. Assessment of re-aggregated human pancreatic islets for secondary drug screening. *Br J Pharmacol*. 2014 Jun;171(12):3010-22. doi: 10.1111/bph.12622. - Ramachandran K, Williams SJ, Huang HH, Novikova L, Stehno-Bittel L. Engineering islets for improved performance by optimized reaggregation in a micromold. *Tissue Eng Part A*. 2013 Mar;19(5-6):604-12. doi: 10.1089/ten.TEA.2012.0553. - Cole C, Burgoyne T, Lee A, Stehno-Bittel L, Zaid G. Arum Palaestinum with isovanillin, linolenic acid and β-sitosterol inhibits prostate cancer spheroids and reduces the growth rate of prostate tumors in mice. *BMC Complement Altern Med*. 2015 Aug 5;15:264. doi: 10.1186/s12906-015-0774-5.

Diversity Certificates

Small business

Our Services (19)


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3D Cell-Based Assays

Price on request

Likarda produces 3D cell-based clusters and assays using a uniform 3D, scaffold-free methodology. Our clusters are all uniform in size, and run on 96 or 384-well plate formats. We're able to produce them from a variety of different cell types in many different species whether primary or cell lines. We've worked in both human and animal health. These assays can be useful for toxicology, metabolism, cell health and a number of other aspects.

Pancreatitis Diabetes Oncology Drug Discovery Cell culture Cell-based assays primary cell culture immortalized cell culture 3D culture scaffold-free 3D culture Cytotoxicity in vitro toxicity heptotoxicity Human Rat Dog Cat Toxicology Mouse Show 19 more tags Show less

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Cell-Based Assays

Price on request

In addition to the 3D cell-based assays that we conduct, we're able to complete tests in 2D on monolayer or suspension cell cultures. We design experiments to match the scientific question posed by the client. We've worked with primary cells, cell lines, xenografts models, and a variety of species including humans, dogs, cat, mouse, and rats. In addition, our scientists are experienced in developing novel assays for clients, and have published new methods for multiplexing and in vitro toxicity assays.

Oncology Diabetes Immuno-Oncology BioTek Cytation 5 Perkin Elmer Enspire Drug Discovery ELISA alphaLISA Immunology Metabolism and Endocrinology Hepatology Toxicology Cell culture Cell-based assays primary cell culture live-cell imaging Cytotoxicity heptotoxicity in vitro toxicity Immunoassays Immunofluorescence Fluorescent labeling immunohistochemistry histology luminescence microscopy Thyroid disease NanoEnTek EVE automated cell counter Zeiss Microscope III RS Nikon Inverted Microscope Eclipse TE200 American Optical Biostar Tissue Culture Microscope Olympus UPlanAPO wet and dry objectives Olympus Fluoview 300 Laser Scanning Confocal Microscope Agilent 1100 Hamilton Microlab Star Automated Liquid Handling System Integra VIAFLO Assist automating system Thermo Scientific Steri-Cycle Direct Heat C02 Incubators GMP Material Storage Mettler Toledo Analytical Balance AG104 Mettler Toledo pH Meter MP225 Ames test Biomarker analysis calcium imaging cell imaging Chemiluminescent Immunoassay comparison testing HPLC Custom Assays efficacy testing Method Validation Method Development Project management scaffold-free 3D culture Stability Studies Show 54 more tags Show less

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Assay Development

Price on request

New therapies that utilize the intact immune system, alter genes, or are comprised of cells as therapy, require complex in vitro assays. Likarda's scientists are experienced in developing and validating new assays, depending on the client's needs. Publications by our scientists, Drs. Linsdey Ott and Karthik Rakmachandran highlight some of the capabilities of the team.

Cell-based assays Autoimmune disease Oncology Diabetes Thyroid disease Hepatology Cardiovascular Nephrology and Urology Respiratory Musculoskeletal/Orthopedic BioTek Cytation 5 Perkin Elmer Enspire NanoEnTek EVE automated cell counter Zeiss AxioVert Epifluorescence Microscope Nikon Inverted Microscope Eclipse TE200 American Optical Biostar Tissue Culture Microscope Olympus Fluoview 300 Laser Scanning Confocal Microscope Agilent 1100 Hamilton Microlab Star Automated Liquid Handling System Integra VIAFLO Assist automating system Thermo Scientific Steri-Cycle Direct Heat C02 Incubators GMP Material Storage Caron Environmental Test Chamber Caron Photostability Test Chamber Eppendorf Centrifuge 5415C Mettler Toledo Analytical Balance AG104 Mettler Toledo pH Meter MP230 Drug Discovery Toxicology Biology Affinity chromatography alphaLISA Amino Acid Analysis dose response Ames Stability Studies Method Development Method Validation ELISA Cell culture cell imaging assay optimization assay development inhibition assessment HPLC High-throughput data analysis 3D culture kinetics multiplex Show 49 more tags Show less

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Spent Media Analysis

Price on request

Likarda offers spent media analysis with the ability to analyze and detect free amino acids, using an LC automated sample analysis and data processing method. This analysis provides an understanding of changes in media conditions over time and provides a detailed understanding of the changes in culture conditions over time. The method provides sensitive and high‐resolution separation of all amino acids in media. Studying the roles of amino acids during bioprocesses leads to better understanding the feeding strategy, and to improving the yield and quality of the media.

amino acids Diabetes Oncology Metabolism and Endocrinology CNS/Neurology Dermatology Dental Musculoskeletal/Orthopedic Perkin Elmer Optima 7000 Agilent 1100 BioTek Cytation 5 Perkin Elmer Enspire Zeiss AxioVert Epifluorescence Microscope Nikon Inverted Microscope Eclipse TE200 Olympus Fluoview 300 Laser Scanning Confocal Microscope Hamilton Microlab Star Automated Liquid Handling System Integra VIAFLO Assist automating system Fisher Isotemp Chromatography Refrigerator Revco +4 Double Door Refrigerator REL5004A20 Caron Environmental Test Chamber Caron Photostability Test Chamber Mettler Toledo Analytical Balance AG104 Bransonic Ultrasonic Bath Sonicator HPLC Metals Analysis Spent Media Analysis Cellular Compound Screening live-cell imaging Cell-based assays analytical chemistry Show 30 more tags Show less

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Cell-Based Compound Screening

Price on request

Likarda's scientific team conducts large- and small-scale compound screens for academia, small start-ups, or large pharmaceutical companies. We utilize liquid handling robots and our patented microplates to conduct high through-put screens in 2D or with 3D cell clusters.

We have extensive experience in searching for synergies or antagonistic relationships between test compounds and a variety of molecules including marketed drugs, environmental factors, or other test compounds. Our proprietary computer programs direct our robotics lab to test 1000s of compound combinations in a rapid time frame, with almost instantaneous results.

Diabetes Oncology Thyroid disease Liver Disease Respiratory Cardiovascular CNS/Neurology Nephrology and Urology BioTek Cytation 5 Perkin Elmer Enspire NanoEnTek EVE automated cell counter Zeiss AxioVert Epifluorescence Microscope Nikon Inverted Microscope Eclipse TE200 American Optical Biostar Tissue Culture Microscope Olympus Fluoview 300 Laser Scanning Confocal Microscope Agilent 1100 Hamilton Microlab Star Automated Liquid Handling System Integra VIAFLO Assist automating system Fisher Isotemp Chromatography Refrigerator -80 Freezer GMP Material Storage Thermo Savant DNA 120 Speed Vac DNA 120-115 Branson Sonifier 250 Drug Discovery Toxicology ELISA Cell culture cell imaging Cell-based assays primary cell culture immortalized cell culture metabolomics Colorimetric Chemiluminescent Immunoassay luminescence alphaLISA multiplex stability GMP proliferation Immunochemistry Method Validation Method Development 3D culture scaffold-free 3D culture High-throughput data analysis drug-drug interactions Show 47 more tags Show less

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Cell Viability Assays

Price on request

Cell viability assays are essential for a variety of research questions. We routinely use colorimetric, luminescent and fluorescent detectors of cell viability. For certain scientific goals, cell function may be a better representation of cell viability. We use standard assays or develop novel cell viability tests, depending on the need.

Cytotoxicity heptotoxicity in vitro toxicity Cell-based assays microscopy live-cell imaging CNS/Neurology Pancreatitis Thyroid disease Cardiovascular Oncology Nephrology and Urology Gastroenterology Diabetes Neurodegenerative diseases Liver Disease Musculoskeletal/Orthopedic Bone disease BioTek Cytation 5 Perkin Elmer Enspire NanoEnTek EVE automated cell counter Zeiss AxioVert Epifluorescence Microscope Nikon Inverted Microscope Eclipse TE200 American Optical Biostar Tissue Culture Microscope Olympus Fluoview 300 Laser Scanning Confocal Microscope Agilent 1100 Hamilton Microlab Star Automated Liquid Handling System Cybio integrated multi device robotics Integra VIAFLO Assist automating system -80 Freezer GMP Material Storage Drug Discovery Toxicology Immunofluorescence Time Resolved Fluorescence immunohistochemistry Show 36 more tags Show less

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Cytotoxicity Assays

Price on request

We utilize a number of cell toxicity assays using standard tests based on fluorescent, luminescent or colorimetric read-outs. Alternatively, we have designed unique multiplexed assays that measured cell toxicity, biomarkers, and cellular metabolic outcomes. These custom assays provide our clients with robust results at a lower cost and with less test compound and less time. Drs. Lindsey Ott and Karthik Rakmachandran have published manuscripts on some of our novel assays.

Toxicity from drug-drug interactions is important information to obtain early in the drug development process. Likarda has proprietary programming that allows our liquid handling instruments to test for drug-drug interactions in a high-throughput manner, and (if needed) in 3D cell culture.

Cytotoxicity heptotoxicity in vitro toxicity live-cell imaging Cell-based assays microscopy Fluorescent labeling Diabetes Oncology Neurodegenerative diseases CNS/Neurology Pancreatitis Thyroid disease Nephrology and Urology Gastroenterology Liver Disease BioTek Cytation 5 Perkin Elmer Enspire NanoEnTek EVE automated cell counter Zeiss AxioVert Epifluorescence Microscope Nikon Inverted Microscope Eclipse TE200 American Optical Biostar Tissue Culture Microscope Olympus Fluoview 300 Laser Scanning Confocal Microscope Agilent 1100 Hamilton Microlab Star Automated Liquid Handling System Integra VIAFLO Assist automating system Thermo Scientific Upright Freezer ULT2540-3-A41 Fisher Isotemp Chromatography Refrigerator Mettler Toledo pH Meter MP230 Mettler Toledo Analytical Balance AG104 Toxicology Drug Discovery 3D culture scaffold-free 3D culture hepatotoxicity drug-drug interactions Time Resolved Fluorescence GMP alphaLISA ELISA luminescence Fluorescence Method Development Method Validation Show 44 more tags Show less

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Cell Death Assays

Price on request

We utilize a number of cell death assays using standard tests based on fluorescent, luminescent or colorimetric read-outs. Alternatively, we have designed unique multiplexed assays that measured cell death along with biomarkers, and cellular metabolic outcomes. These custom assays provide our clients with robust results at a lower cost and with less test compound and less time. Drs. Lindsey Ott and Karthik Rakmachandran have published manuscripts on some of our novel assays.

Toxicity from drug-drug interactions is important information to obtain early in the drug development process. Likarda has proprietary programming that allows our liquid handling instruments to test for drug-drug interactions in a high-throughput manner, and (if needed) in 3D cell culture.

Diabetes Oncology CNS/Neurology Musculoskeletal/Orthopedic Cardiovascular Liver Disease Nephrology and Urology Metabolism and Endocrinology BioTek Cytation 5 Perkin Elmer Enspire NanoEnTek EVE automated cell counter Zeiss AxioVert Epifluorescence Microscope Nikon Inverted Microscope Eclipse TE200 American Optical Biostar Tissue Culture Microscope Olympus Fluoview 300 Laser Scanning Confocal Microscope Agilent 1200 Hamilton Microlab Star Automated Liquid Handling System Integra VIAFLO Assist automating system Thermo Scientific Steri-Cycle Direct Heat C02 Incubators Fisher Isotemp Chromatography Refrigerator Revco +4 Double Door Refrigerator REL5004A20 GMP Material Storage Caron Environmental Test Chamber Caron Photostability Test Chamber ThermoSavant Speedvac DNA120-115 Drug Discovery Toxicology ELISA alphaLISA Fluorescence Colorimetric Chemiluminescent Immunoassay Immunoassays Cell culture live-cell imaging HPLC Cell-based assays drug-drug interactions in vitro assays in vitro toxicity High-throughput data analysis Screening Show 42 more tags Show less

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Cell Viability & Proliferation Assays

Price on request

Cell viability assays are essential for a variety of research questions. We routinely use colorimetric, luminescent and fluorescent detectors of cell viability. For certain scientific goals, cell function may be a better representation of cell viability. We use standard assays or develop novel cell viability tests, depending on the need.

CNS/Neurology Cardiovascular Metabolism and Endocrinology Diabetes Oncology Immunology Musculoskeletal/Orthopedic Liver Disease Nephrology and Urology BioTek Cytation 5 Perkin Elmer Enspire NanoEnTek EVE automated cell counter Zeiss AxioVert Epifluorescence Microscope Nikon Inverted Microscope Eclipse TE200 American Optical Biostar Tissue Culture Microscope Olympus Fluoview 300 Laser Scanning Confocal Microscope Agilent 1200 Hamilton Microlab Star Automated Liquid Handling System Integra VIAFLO Assist automating system Thermo Savant DNA 120 Speed Vac DNA 120-115 Thermo Scientific Steri-Cycle Direct Heat C02 Incubators Fisher Isotemp Chromatography Refrigerator Thermo Forma CryoMed Control Rate Freezer 7452 Revco +4 Double Door Refrigerator REL5004A20 GMP Material Storage Caron Environmental Test Chamber Caron Photostability Test Chamber Eppendorf 5810R Refrigerated Centrifuge Mettler Toledo Analytical Balance AG104 Drug Discovery Toxicology Cell culture cell imaging live-cell imaging primary cell culture immortalized cell culture ELISA Colorimetric luminescence Fluorescence Method Development Method Validation GMP Show 43 more tags Show less

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Apoptosis Assays

Price on request

We utilize a number of cell toxicity assays using standard tests based on fluorescent, luminescent or colorimetric read-outs. Alternatively, we have designed unique multiplexed assays that measured cell toxicity, biomarkers, and cellular metabolic outcomes. These custom assays provide our clients with robust results at a lower cost and with less test compound and less time. Drs. Lindsey Ott and Karthik Rakmachandran have published manuscripts on some of our novel assays.

Toxicity from drug-drug interactions is important information to obtain early in the drug development process. Likarda has proprietary programming that allows our liquid handling instruments to test for drug-drug interactions in a high-throughput manner, and (if needed) in 3D cell culture.

Metabolism and Endocrinology Oncology CNS/Neurology Cardiovascular Pancreatitis Dermatology Diabetes BioTek Cytation 5 Perkin Elmer Enspire NanoEnTek EVE automated cell counter Zeiss AxioVert Epifluorescence Microscope Nikon Inverted Microscope Eclipse TE200 American Optical Biostar Tissue Culture Microscope Olympus Fluoview 300 Laser Scanning Confocal Microscope Agilent 1200 Hamilton Microlab Star Automated Liquid Handling System Integra VIAFLO Assist automating system Thermo Scientific Steri-Cycle Direct Heat C02 Incubators Fisher Isotemp Chromatography Refrigerator Revco +4 Double Door Refrigerator REL5004A20 GMP Material Storage Caron Photostability Test Chamber Caron Environmental Test Chamber Branson Sonifier 250 ThermoSavant Speedvac DNA120-115 Drug Discovery Toxicology Cell culture live-cell imaging Cell-based assays immortalized cell culture primary cell culture ELISA Colorimetric Fluorescence luminescence HPLC Metals Analysis Show 38 more tags Show less

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Biostatistical Analysis

Price on request

Review of Biostatistical Reports and provide Biostatistical Analysis support.


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Grant Writing

Price on request

Staff of Likarda have academic experience maintaining a research laboratory on external grants for over 20 years, and was successful in obtaining SBIR and STTR grants, NIH R01s and other R series grants, Dept. of Commerce grants, funding from private non-profit organization, and industry grants. Likarda can serve in a variety of capacities concerning grants by writing the grant, serving as consultants, and even submitting the grant for the client.

Oncology Diabetes Metabolism and Endocrinology Cardiovascular Musculoskeletal/Orthopedic Nephrology and Urology Liver Disease CNS/Neurology Dermatology Show 9 more tags Show less

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Technical Writing

Price on request

Each member of the scientific team at Likarda has an average of more than 15 peer-reviewed manuscripts in journals including Science, Neuron, Journal of Biological Chemistry, American Journal of Physiology, and the Tissue Engineering. Most of our published work is based on our own independent research. In addition, we serve as ghost writers for our clients who need assistance with writing. We have experience in writing white papers for our clients, and have created slide decks for public presentations such as at public meetings or for potential investors. We also have the ability to provide QC of reports and technical documentation.

Metabolism and Endocrinology Diabetes Oncology Musculoskeletal/Orthopedic Cardiovascular CNS/Neurology Liver Disease Nephrology and Urology Immuno-Oncology Immunology Dermatology Show 11 more tags Show less

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Non-Regulatory Technical Document Review

Price on request

Report Quality Control

Provide QC services for reviewing reports and other technical documents.


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Scientific Manuscript Preparation

Price on request

Each member of the scientific team at Likarda has an average of more than 15 peer-reviewed manuscripts in journals including Science, Neuron, Journal of Biological Chemistry, American Journal of Physiology, and the Tissue Engineering. Most of our published work is based on our own independent research. In addition, we serve as ghost writers for our clients who need assistance with writing.

Oncology Diabetes Metabolism and Endocrinology Cardiovascular Dermatology CNS/Neurology Immuno-Oncology Immunology Liver Disease Nephrology and Urology Musculoskeletal/Orthopedic Show 11 more tags Show less

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Editorial and Writing Services

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Product Development, Testing, and Packaging

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Product Development & Testing Services


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Specialized Cell-Based Assays

Price on request
Request a quote for more information about this service.

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Scientific Manuscript Editing

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KR

Karthik Ramachandran

Vice President of Strategic Growth and New Business Development
LS

Lisa Stehno-Bittel

President

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