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LifeTein

ic 8.8/10 Overall Rating 95% Positive
ic 83 Orders Completed
South Plainfield, New Jersey, US

About LifeTein

Founded: 2008 Type: Privately Held Size: 11-50 employees

LifeTein LLC, located in New Jersey, was founded for its global custom peptide synthesis services, antibody production services, gene synthesis, protein production and chemical services.

LifeTein provides the fastest turnaround time and most reliable quality in the industry. Projects move from conception to... Show more »

LifeTein LLC, located in New Jersey, was founded for its global custom peptide synthesis services, antibody production services, gene synthesis, protein production and chemical services.

LifeTein provides the fastest turnaround time and most reliable quality in the industry. Projects move from conception to bench in only 3–5 days so you can deal with your research deadlines.

3–5 Day Delivery with RUSH Peptide Synthesis Service. Made in the USA.

Rush < 20 amino acids, crude purity in 3–5 days;

Rush < 20 amino acids, >95% purity in 6–10 days;

Rush 20–40 amino acids, >95% in 1–2 weeks.

Recent Publications:
1. Nature Communications, Volume 9, Article number: 607 (2018), Cellular imaging by targeted assembly of hot-spot SERS and photoacoustic nanoprobes using split-fluorescent protein scaffolds
2. Nature, Available online on 17 January 2018, doi:10.1038/nature25442, Monitoring T cell–dendritic cell interactions in vivo by intercellular enzymatic labelling
3. Cell, Available online 11 January 2018, LXR/ApoE Activation Restricts Innate Immune Suppression in Cancer
4. Science Advances, Park et al., Sci. Adv. 2018; 4: e1601453 12 January 2018, Membrane insertion of—and membrane potential sensing by—semiconductor voltage nanosensors: Feasibility demonstration
5. Nature Scientific Reports, Article number: 14157 (2017), Connexin 43 Hemichannels Regulate the Expression of Wound Healing-Associated Genes in Human Gingival Fibroblasts
6. Nature Communications, 8, Article number: 1403 (2017), Identification of a peptide recognizing cerebrovascular changes in mouse models of Alzheimer’s disease

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Diversity Certificates

Woman owned small business

Our Services (24)


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Peptide Synthesis

Price on request

LifeTein has overhauled the entire peptide synthesis process and developed a proprietary method that delivers purified peptides of typical length in 3–5 days versus several weeks. High-purity product takes about one week.

Aside from common side chain modifications (“unnatural” amino acids), LifeTein produces peptides with... Show more »

LifeTein has overhauled the entire peptide synthesis process and developed a proprietary method that delivers purified peptides of typical length in 3–5 days versus several weeks. High-purity product takes about one week.

Aside from common side chain modifications (“unnatural” amino acids), LifeTein produces peptides with modifications include oligo-peptide conjugation, phosphorylation, biotinylation, cyclic peptides (head-to-tail, disulfide bond), cell penetrating peptides, stable isotopes 13C/15N, stapled peptides, D-amino acids, FRET, and fluorescent peptides using FITC, FAM, TAMRA, Rhodamine, Cy3, Cy5, Cy7, and Alexa dyes.

Rest assured that your rush peptide orders are made in New Jersey, 100% accurate and compliant with all intellectual property laws.

LifeTein provides the fastest turnaround time and most reliable quality in the industry. Projects move from conception to bench in only 3–5 days so you can deal with your research deadlines. The rush service fee applies.

Rush Delivery (Business Days):

  • Rush < 20 amino acids, crude purity, 3–5 days

  • Rush < 20 amino acids, >95% purity, 6–10 days

  • Rush 20–40 amino acids, >95%, 1–2 weeks

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Analytical HPLC Agilent 1200 Merrifields peptide resin venom peptides Immunology Oncology Phosphopeptide Enrichment Peptide Map chemical modification protein quantification Biochemical Compound Screening Peptide mapping Mass Spectrometry glycoprotein characterization Protein-protein interaction networks peptide labeling peptide conjugation Biology immunogen peptide antibody antigen Show 22 more tags Show less

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Oligonucleotide Modification

Price on request

LifeTein Peptide oligonucleotide conjugate: DNA-Peptide and RNA-Peptide. Oligonucleotide-peptide conjugates are chimeric molecules composed of a nucleic acid moiety covalently linked to a peptide moiety. It is an important tool for studies of the protein– and peptide–nucleic acid interactions, as well as being promising bioconjugates for nucleic acid delivery and therapy.

LifeTein Peptide oligonucleotide conjugate: DNA-Peptide and RNA-Peptide. Oligonucleotide-peptide conjugates are chimeric molecules composed of a nucleic acid moiety covalently linked to a peptide moiety. It is an important tool for studies of the protein– and peptide–nucleic acid interactions, as well as being promising bioconjugates for nucleic acid delivery and therapy.

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Cell-based assays live-cell imaging Drug-Gene Regulation Small molecules

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Polyclonal Antibody Production

Starting at $850.00 per sample

LifeTein provides a complete portfolio of antibody services including free peptide antigen design, peptide synthesis and polyclonal and monoclonal production, development, and purification services. The antibodies will be purified by the peptide affinity column for higher concentration and specification.

LifeTein's AdjuBooster... Show more »

LifeTein provides a complete portfolio of antibody services including free peptide antigen design, peptide synthesis and polyclonal and monoclonal production, development, and purification services. The antibodies will be purified by the peptide affinity column for higher concentration and specification.

LifeTein's AdjuBooster adjuvant was developed to increase immune response in a shorter time.
Other services including: phosphor-specific antibody services, methylation-specific antibody services, epitope tag antibodies.

  1. Peptide antigen design (free), Peptide synthesis: >75% purity, 10 mg,
  2. Conjugation of peptide to KLH (2-3 mg)
  3. Standard 12-15 week antibody production schedule including 4-5 immunizations with 2 rabbits
  4. Peptide affinity column purification
  5. Deliverables: 2-3 mg peptides, 1-2 ml pre-immune sera, 2-5 mg purified antibodies, Guaranteed ELISA results (>1:32,000)
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Agilent 1200 Rabbit Guinea pig Mouse Rat Polyclonal antibodies Oncology western blot Phosphopeptide Enrichment Polyclonal Peptide Map monoclonal Peptide mapping Mass Spectrometry Western blotting Antibodies Proteins Monoclonal antibodies milligram Bacteria Show 20 more tags Show less

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Custom Gene Synthesis

Price on request

Gene Synthesis Services: Subclone to your own vector at competitive price. By default, we clone genes into our pUC19 or pUC57 vector for free, subcloning will add additional two days.

Free codon optimization: Optimized genes have higher protein expression in their correspondent hosts.

Fast turnaround time. 1-2 weeks for <1.5 kb... Show more »

Gene Synthesis Services: Subclone to your own vector at competitive price. By default, we clone genes into our pUC19 or pUC57 vector for free, subcloning will add additional two days.

Free codon optimization: Optimized genes have higher protein expression in their correspondent hosts.

Fast turnaround time. 1-2 weeks for <1.5 kb genes. 2-3 weeks for genes of 1.5 kb to 5 kb.

Advanced synthetic technology for complex genes with repetitive, AT or GC rich DNA.

Order your Gene Synthesis Service at a competitive price! Now it is 30% off.
Gene Length Turnaround List Price

<1.5 kb 2-3 weeks $0.25/bp, 30% Off Promotion: *$0.18/bp
1.5-3 kb 2-4 weeks $0.25/bp, 30% Off Promotion: *$0.18/bp
3-7 kb 4-6 weeks Please Inquire
>7 kb 7-8 weeks Please Inquire

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Genetic Disease HPLC gene editing Next-Generation Proteomics gene delivery gene synthesis Drug Discovery Genomics Filtered Gene List Bacteria Show 10 more tags Show less

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Gene Synthesis

Price on request

LifeTein® offers gene synthesis service, protein expression in E.coli. and Baculovirus/Insect cell systems. Protein expression in bacterial cells is a fast and economical way for high levels of recombinant proteins. Baculovirus expression system has the advantages of high-level expression and ease of scale-up with... Show more »

LifeTein® offers gene synthesis service, protein expression in E.coli. and Baculovirus/Insect cell systems. Protein expression in bacterial cells is a fast and economical way for high levels of recombinant proteins. Baculovirus expression system has the advantages of high-level expression and ease of scale-up with posttranslational modifications.

Order your Gene Synthesis Service at a competitive price! Now it is now only $0.18/bp after 30% off. Email us at [email protected] for a quote.

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gene delivery gene synthesis gene tagging

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Chemical Synthesis

Price on request

LifeTein provides medicinal chemistry support services for the synthesis of target compounds, reference compounds, lead compound derivatives, specialized reagents, and other compounds of interest, some samples: small molecules, API's, complex intermediates and final targets, combinatorial building blocks, resynthesis, multi-step... Show more »

LifeTein provides medicinal chemistry support services for the synthesis of target compounds, reference compounds, lead compound derivatives, specialized reagents, and other compounds of interest, some samples: small molecules, API's, complex intermediates and final targets, combinatorial building blocks, resynthesis, multi-step syntheses, libraries.

Features and Benefits

  • Process Research, Development, and Optimization: Research and development of synthetic pathways for the production of even the most complex molecules and multi-step synthesis pathways;
  • Synthetic Route Design and Optimization: Full literature searches, ensuring that the synthetic route we create for you is the most efficient and optimized route possible
  • Small- and Large-Scale Chemistry Capabilities: Custom synthesis in quantities ranging from milligrams to multi-kilogram orders. We help you bring your projects from idea to production
  • Advanced Synthetic and Analytical Platform: Our chemists work in well-equipped facilities with full access to scientific literature and the reaction databases. We can handle complex distillation, purification, and scale-up protocols
  • Expert Project Management: We keep you up to date on the progress of your projects every step of the way! Expect weekly project updates as your order moves toward completion
  • Green Chemistry Synthesis: We have replaced dangerous and environmentally unfriendly steps during chemical synthesis
  • Low Cost and High Efficiency: We carefully reduced the number of steps to lower costs and increase the efficiency, yield, and throughput of the process
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Chemical Synthesis Physiochemical Testing chemical modification Biochemical Compound Screening Chemical Libraries for DEL Technology Agrochemicals gram milligram kilogram Show 9 more tags Show less

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Synthetic Chemistry

Price on request
Request a quote for more information about this service.

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Chemistry and Materials

Price on request
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Peptide Libraries

Starting at $500.00 per sample

Peptide libraries are synthesized on LifeTein’s unique proprietary synthesis platform. Our process includes unpurified crude peptide libraries (Purity varies from 30% up to 80% depending on the sequence) and fully-purified peptides (70%, 80%, 90%, or 95% purity using reverse-phase HPLC).

Our library types include overlapping,... Show more »

Peptide libraries are synthesized on LifeTein’s unique proprietary synthesis platform. Our process includes unpurified crude peptide libraries (Purity varies from 30% up to 80% depending on the sequence) and fully-purified peptides (70%, 80%, 90%, or 95% purity using reverse-phase HPLC).

Our library types include overlapping, truncation peptide library, alanine peptide scanning library, random peptide library, scrambled peptide library, and positional peptide library.

Library Types
Overlapping Peptide Library: Individual peptides can be divided in to several fragments that overlap. The resulting overlapping peptide libraries can then be used for processes including continuous and linear epitope mapping.

Truncation Peptide Library: Peptides can be cleaved systematically into small fragments. The library of truncated peptides could then be used to predict the minimum length amino acid that could be used to achieve optimal epitope activity.

Alanine Peptide Scanning Library: Each amino acid is substituted individually and systematically for alanine. Alanine scanning allows the easy identification of the specific amino acids that are responsible for the conformation, activity, and function of a protein.

Random Peptide Library: Selected positions are substituted with all 20 natural amino acids simultaneously, which might increase peptide activity.

Scrambled Peptide Library: These libraries are designed using variations of the original sequence of a peptide. The resulting peptides are used generally as negative controls to show that a specific sequence is critical to the protein function or activity. It is also a random screening tool used to find new leads.

Positional Peptide Library: Selected sites or regions within a peptide sequence are replaced systematically with other amino acids. These libraries facilitate the identification of the positions and regions that are responsible for specific activities or effects.

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Phosphopeptide Enrichment Peptide Map Peptide mapping

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Bacterial Protein Expression

Price on request

LifeTein® offers gene synthesis service, protein expression in E.coli. and Baculovirus/Insect cell systems. Protein expression in bacterial cells is a fast and economical way for high levels of recombinant proteins. Baculovirus expression system has the advantages of high-level expression and ease of scale-up with... Show more »

LifeTein® offers gene synthesis service, protein expression in E.coli. and Baculovirus/Insect cell systems. Protein expression in bacterial cells is a fast and economical way for high levels of recombinant proteins. Baculovirus expression system has the advantages of high-level expression and ease of scale-up with posttranslational modifications.

Bacterial Expression System: Different optimized conditions are used for clones in order to find the optimum soluble expression condition with our system. The pET expression system provides high level of protein production.

E. coli system for soluble protein with high stability

Classic ploy-Histidine tag for most protein of interest

High affinity FLAG or GST tag for protein with very low expression level

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Protein Services

Price on request

Transient Expression Services in the New Jersey lab Facility

Transient expression in mammalian cells is crucial for proteins requiring post-translational modifications and appropriate protein folding. LifeTein is a world-leading expert in transient expression systems with the lab facility in New Jersey.

LifeTein's proprietary... Show more »

Transient Expression Services in the New Jersey lab Facility

Transient expression in mammalian cells is crucial for proteins requiring post-translational modifications and appropriate protein folding. LifeTein is a world-leading expert in transient expression systems with the lab facility in New Jersey.

LifeTein's proprietary transient mammalian expression technology renders optimal protein and antibody yields (up to gram scale) for your projects. Our optimized expression protocol guarantees gram-level recombinant protein expression.

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monoclonal antibody cell lines Cell culture Cell line engineering Protein-Protein, Protein-Nucleic Acid complex analysis bacterial based recombinant protein production cell line generation cell line development Protein Show 9 more tags Show less

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Protein Production

Price on request
Request a quote for more information about this service.

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Monoclonal Antibody (mAb) Production

Starting at $4,900.00 per sample

LifeTein provides a complete portfolio of antibody services including free peptide antigen design, peptide synthesis and polyclonal and monoclonal production, development, and purification services.
LifeTein's AdjuBooster adjuvant was developed to increase immune response in a shorter time.
Other services including:... Show more »

LifeTein provides a complete portfolio of antibody services including free peptide antigen design, peptide synthesis and polyclonal and monoclonal production, development, and purification services.
LifeTein's AdjuBooster adjuvant was developed to increase immune response in a shorter time.
Other services including: phosphor-specific antibody services, methylation-specific antibody services, epitope tag antibodies.

  1. Include peptide design (free) and peptide synthesis, immunization and sera collection, ELISA test.
  2. Splenocyte fusions and subclone, ascites production and purification.
  3. Standard 20-26-week antibody production schedule
  4. Deliverables: 2-3 mg peptides, 50 ul pre-immune sera, 2 clones, 2-5 mg purified antibodies (2 clones), ELISA results >1:32,000, isotype determination

Rabbit monoclonal antibody services from any immunogens to monoclonal antibodies in four months

Immunize two rabbits with recombinant protein or conjugated peptide/hapten (60 days)
* Isolate PMBC or splenocytes from selected rabbit (14 days)
* Screen and culture antibody-secreting B-cells
* Clone variable regions of rabbit lgG genes from identified specific B lymphocytes
* Subclone heavy and light chain genes into expression vectors (30 days)
* Validate express recombinant rabbit mAb’s blinding to antigen by indirect ELISA

Deliverables: Five clones. Full length heavy & light chain genes in non-proprietary plasmid. 100ug purified antibodies

Rabbit Monoclonal Antibodies based on a single cell platform with a shorter screen time
- Strong Affinity: The rabbit monoclonal antibodies are 10-100 times higher affinity than mouse monoclonal antibodies.
- High Specificity: The rabbit monoclonal antibodies have improved sensitivity for assays with lower false positive signals.
- Improved Stability: The rabbits IgGs have extra disulfide bonds for improved stability and shelf life.
- Broader epitope recognition: The rabbit antibodies recognize small molecules or haptens including lipids, hormones and small drugs. The antibodies can be raised for peptides with modifications, protein with conformational epitopes or enzyme with cleavage sites.

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Anti-GST Antibody monoclonal antibody Antibody

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Antibody Services

Price on request

Rabbit monoclonal antibody services from any immunogens to monoclonal antibodies in four months
Immunize two rabbits with recombinant protein or conjugated peptide/hapten (60 days)

Isolate PMBC or splenocytes from selected rabbit (14 days)
Screen and culture antibody-secreting B-cells
Clone variable regions of rabbit lgG genes... Show more »

Rabbit monoclonal antibody services from any immunogens to monoclonal antibodies in four months
Immunize two rabbits with recombinant protein or conjugated peptide/hapten (60 days)

Isolate PMBC or splenocytes from selected rabbit (14 days)
Screen and culture antibody-secreting B-cells
Clone variable regions of rabbit lgG genes from identified specific B lymphocytes
Subclone heavy and light chain genes into expression vectors (30 days)
Validate express recombinant rabbit mAb’s blinding to antigen by indirect ELISA

Deliverables: Five clones. Full length heavy & light chain genes in a non-proprietary plasmid. 100ug purified antibodies.

Rabbit Monoclonal Antibodies based on a single cell platform with a shorter screen time

Strong Affinity: The rabbit monoclonal antibodies are 10-100 times higher affinity than mouse monoclonal antibodies.

High Specificity: The rabbit monoclonal antibodies have improved sensitivity for assays with lower false positive signals.

Improved Stability: The rabbit's IgGs have extra disulfide bonds for improved stability and shelf life.

Broader epitope recognition: The rabbit antibodies recognize small molecules or haptens including lipids, hormones and small drugs. The antibodies can be raised for peptides with modifications, the protein with conformational epitopes or enzyme with cleavage sites.

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Assay Development

Price on request

Lifetein provides gene, peptide, protein, antibody, and chemicals for the assay development. Please send your project and we will provide the proposal for your assay development.

Lifetein provides gene, peptide, protein, antibody, and chemicals for the assay development. Please send your project and we will provide the proposal for your assay development.

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Biochemistry & Molecular Biology

Price on request
Request a quote for more information about this service.

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Biology

Price on request
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Recombinant Protein Expression

Price on request
Request a quote for more information about this service.

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Antibody Modification

Price on request
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Antibody Purification

Price on request

LifeTein's new transient protein expression services offer flexible and rapid production of your functional protein or antibodies in CHO or HEK293 cells. We have broad experience in pilot scale, small scale, and large scale mammalian cell culture of hybridomas for production of monoclonal antibodies. Our facility is equipped with... Show more »

LifeTein's new transient protein expression services offer flexible and rapid production of your functional protein or antibodies in CHO or HEK293 cells. We have broad experience in pilot scale, small scale, and large scale mammalian cell culture of hybridomas for production of monoclonal antibodies. Our facility is equipped with tissue culture bags, roller bottles, bioreactors, hollow fiber systems, and stirred tank fermenters. Our team will help you choose the best and most cost effective method to produce your monoclonal antibodies.

Key Features:

Choice of HEK293 or CHO expression

Optimized protocol for difficult and low-yield proteins

Yield improvements of up to 80 fold

Short turnaround time of 10 weeks

Up to 2g/L

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Mouse Monoclonal Antibody Isotyping Reagents monoclonal antibody monoclonal antibodies Antibodies Monoclonal antibodies

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Bioconjugation/Chemical Crosslinking

Price on request

Peptide-oligo (RNA, DNA, siRNA) conjugate,
Peptide-antibody conjugate,
Peptide-compound conjugate,
Peptide-protein conjugate
Peptide-peptide conjugate
Click chemistry for all possible cross-linking and conjugations

Peptide-oligo (RNA, DNA, siRNA) conjugate,
Peptide-antibody conjugate,
Peptide-compound conjugate,
Peptide-protein conjugate
Peptide-peptide conjugate
Click chemistry for all possible cross-linking and conjugations

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Hybridoma Subcloning

Price on request
Request a quote for more information about this service.

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Hybridoma Fusion

Price on request
Request a quote for more information about this service.

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Hybridoma Screening

Price on request
Request a quote for more information about this service.

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James Chou

Director of Sales and Marketing
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Andy Han

Account Manager
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Cynthia Lu

Account Manager
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Lifetein Services.pdf

  • Positive review for Peptide Synthesis:

    February 2017

    "order is completed in a timely manner. Very good communication from the start to completion. Thank you!"

  • Positive review for Polyclonal Antibody Production:

    January 2017

    "Excellent and expedient service. We used our polyclonal ab for western blotting of endogenous protein in cell lysates and it worked great with absolutely no background. We have CRISPR KOs cell lines to show that the specificity of the antibody is perfect! This was a major roadblock in our work that has been solved. Thank you!"

  • Positive review for Chemical Synthesis:

    December 2016

    "I cannot recommend Life Tein enough. They were fantastic to work with and our laboratory will continue to contract with them in the future. Life Tein was the lowest bidder for two compounds. One compound was fairly simple in synthesis, whereas the other was quite complicated. The easy to synthesize compound was made in a timely manner. We had concerns about the identity of the compound due to a difference in LCMS data than what we expected. Life Tein offered without asking to send a small aliquot of the compound to us to ease our concerns. In the end, Life Tein was correct and had produced a highly pure product and sent the rest of the preparation to us within a week. The more complicated product was difficult to purify; however, the company tried many methods of purification that I believe goes above and beyond other synthesis companies I have dealt with. I would recommend them to anyone looking for a synthesis company!"

  • Positive review for Peptide Synthesis:

    September 2016

    "LifeTein delivered on time and it was very easy to work with them. Communication was great and the price was right. I would definitely work with them again."

  • Positive review for Chemical Synthesis:

    September 2016

    "Great service, fast turn around time, competitive pricing. Will definitely work with LifeTein again for custom organic synthesis. Ewa"

  • Positive review for Peptide Synthesis:

    May 2016

    "The service was great and timely and the products appear of good quality. The customer service was also great and James Chou was always available to give updates and advice. Certainly a positive experience."

  • Positive review for Peptide Synthesis:

    May 2015

    "We worked with LifeTein on the synthesis of a short peptide for part of Study 15 of the Reproducibility Project: Cancer Biology. James was communicative and especially helpful at hammering out the details of our requirements, and helped us write up the protocols for the Registered Report. They delivered the peptide on time, and kept us in the loop as the synthesis was proceeding. We highly recommend James and LifeTein for peptide synthesis!"

  • Positive review for Chemical Synthesis:

    April 2015

    "Working with James was great! He is fast and professional and delivers high quality compounds. We would definitely work with him again."

  • Positive review for Peptide Synthesis:

    December 2013

    "Extremely helpful, responsive and quick. Would highly recommend."

  • Positive review for Peptide Synthesis:

    July 2013

    "Excellent - James was highly responsive and provided high quality peptides quickly and cost effectively. Would definitely recommend."

  • Positive review for Peptide Synthesis:

    January 2013

    "Haven't tested the peptides yet but so far so good. No problems encountered."

LifeTein has not received any endorsements.