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LifeCanvas Technologies, Inc.

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Cambridge, Massachusetts, US

About LifeCanvas Technologies, Inc.

Founded by CLARITY (Nature, 2013), SWITCH (Cell, 2015), and SHIELD (Nature Biotechnology, 2018) inventors from MIT and located in Cambridge, MA, LifeCanvas Technologies develops and offers as services a full suite of research tools for tissue clearing, labeling, and volumetric imaging of intact organs such as... Show more »

Founded by CLARITY (Nature, 2013), SWITCH (Cell, 2015), and SHIELD (Nature Biotechnology, 2018) inventors from MIT and located in Cambridge, MA, LifeCanvas Technologies develops and offers as services a full suite of research tools for tissue clearing, labeling, and volumetric imaging of intact organs such as the brain. We are excited to show the biomedical research community the power of our whole-sample analysis methods, which provide greater value and richer information content versus traditional thin-section techniques, all at a competitive price.

We offer a Full-Service package that replaces embedding, destructive sectioning, staining, and serial imaging of thin tissue slices with a streamlined approach using intact organ samples that offers greater flexibility and requires less hands-on time. With the LifeCanvas pipeline of proprietary technologies, take advantage of whole-sample methods to:
1. Reduce processing work and view samples in multiple anatomical planes
2. Localize regions of interest more confidently by visualizing organ-sized datasets that provide greater context
3. Acquire data on all sample regions in parallel, facilitating analyses that are more quantitatively robust to be performed
4. Explore fertile ground where novel and unexpected discoveries can take place

To learn more, please read our blog post "Why use intact samples for your research?" at https://lifecanvastech.com/blog/
On our website, you can also view videos (https://lifecanvastech.com/video-gallery/) and figures (https://lifecanvastech.com/image-gallery/) of datasets we have generated

We are now happy to offer prospective customers the opportunity to evaluate our services with a sample of their own, free of charge*.
*Conditions apply, as detailed in the Information Intake form linked below.

To provide us with detailed information about your request, please download our Information Intake form and upload it with your quote request. The form is available at the following link:
* .docx (navigating to page will start download of the form) https://lifecanvastech.com/lct-service-information-intake-form/

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Our Services (33)


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Tissue Processing

Price on request

Whole-organ tissue processing is a specialized and time-intensive process that affords researchers a new and unique perspective on anatomical analyses. At LifeCanvas Technologies, we help you reach your research goals by providing an integrated tissue-processing service that is built upon our end-to-end pipeline of technology... Show more »

Whole-organ tissue processing is a specialized and time-intensive process that affords researchers a new and unique perspective on anatomical analyses. At LifeCanvas Technologies, we help you reach your research goals by providing an integrated tissue-processing service that is built upon our end-to-end pipeline of technology solutions.

Utilizing our proprietary devices and reagents, we carry out:
(a) whole-organ tissue preservation (SHIELD), to protect endogenous fluorescence and preserve tissue antigenicity and architecture;
(b) whole-organ delipidation / clearing (SmartClear 2 Pro), to remove light-scattering membrane lipids to enhance penetration of light and exogenous molecular probes;
(c) whole-organ labeling (SmartLabel), to enable visualization of proteins and the structures they identify deep within the organ; and,
(d) light-sheet microscopy (SmartSPIM), for fast acquisition of high-resolution images.

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Tissue Preparation

Price on request

Delipidating tissue to enable intact-sample labeling and imaging involves exposing samples to harsh treatments like high temperatures and pH changes. If left unchecked, this can cause damage to proteins and overall tissue structure, leading to incomplete or inaccurate image datasets. Therefore, to preserve the sample’s endogenous... Show more »

Delipidating tissue to enable intact-sample labeling and imaging involves exposing samples to harsh treatments like high temperatures and pH changes. If left unchecked, this can cause damage to proteins and overall tissue structure, leading to incomplete or inaccurate image datasets. Therefore, to preserve the sample’s endogenous fluorescence and antigenicity along with tissue architecture, we use a novel tissue preservation technique called SHIELD that forms intramolecular bonds using polyfunctional, flexible epoxides to stabilize tissue architecture.

After SHIELD preservation, the tissue can be delipidated and either refractive index-matched for imaging or subjected to active immunohistochemical labeling. Notably, SHIELD-preserved tissues are well-suited for antibody multiplexing, i.e., iterative staining and de-staining, without loss of tissue antigenicity, to build up a rich picture of protein expression over repeated rounds of imaging.

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Tissue Clearing

Price on request

Eliminating membrane lipids is crucial to enabling better light penetration for imaging and increasing tissue permeability for active transport of molecular probes deep into intact tissue. Our active clearing device, SmartClear Pro 2, employs a patent-pending stochastic electrotransport mechanism to foster rapid delivery of... Show more »

Eliminating membrane lipids is crucial to enabling better light penetration for imaging and increasing tissue permeability for active transport of molecular probes deep into intact tissue. Our active clearing device, SmartClear Pro 2, employs a patent-pending stochastic electrotransport mechanism to foster rapid delivery of exogenous molecules such as the detergent SDS into tissues, facilitating uniform removal of light-scattering membrane lipids. Thanks to the application of a rotational electric field which minimizes the displacement of structural biomolecules, the tissue is cleared without any damage or deformation.

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Immunostaining Experimental Design

Price on request

Attempting to passively immunolabel samples that are a few millimeters to one centimeter thick usually takes many weeks and very high concentrations of expensive antibodies, without any guarantee that staining will extend to the sample’s innermost structures.

Our SmartLabel device combines two technologies – stochastic... Show more »

Attempting to passively immunolabel samples that are a few millimeters to one centimeter thick usually takes many weeks and very high concentrations of expensive antibodies, without any guarantee that staining will extend to the sample’s innermost structures.

Our SmartLabel device combines two technologies – stochastic electrotransport and SWITCH – and allows us to achieve whole-organ antibody staining that is uniform from surface to core. While stochastic electrotransport provides for efficient distribution of antibodies into the organ, SWITCH controls reaction kinetics to ensure that antibody binding isn’t activated until reagent concentration has been homogenized throughout the sample. The result is strikingly uniform labeling that enables you to visualize proteins deep within the organ and study the fine-scale topography of the cells they identify.

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Optical Tissue Clearing

Price on request

After clearing and optional labeling, translucent, delipidated samples are incubated in EasyIndex, our refractive index (RI)-matching solution, raising and homogenizing their RI and turning them fully transparent. This increases light penetration into the sample and ensures that images will exhibit the highest resolution and... Show more »

After clearing and optional labeling, translucent, delipidated samples are incubated in EasyIndex, our refractive index (RI)-matching solution, raising and homogenizing their RI and turning them fully transparent. This increases light penetration into the sample and ensures that images will exhibit the highest resolution and focus. The cleared and labeled samples are then embedded in an agarose gel to stabilize them while imaging, ensuring alignment across channels and aiding colocalization of signals.

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Light Sheet Microscopy

Price on request

Acquiring high resolution, three-dimensional volumetric image data of whole organs using confocal or two-photon microscopy is a time-consuming and expensive process, as these slow line-scanning techniques are best suited to small, localized regions of interest. Light-sheet microscopy overcomes this speed limitation by selectively... Show more »

Acquiring high resolution, three-dimensional volumetric image data of whole organs using confocal or two-photon microscopy is a time-consuming and expensive process, as these slow line-scanning techniques are best suited to small, localized regions of interest. Light-sheet microscopy overcomes this speed limitation by selectively illuminating distinct focal planes sequentially from the sides of the tissue sample to achieve optical sectioning. LifeCanvas Technologies’ own light-sheet microscope, SmartSPIM, offers superior imaging speed and uniform axial resolution across the entire sample, generating data-sets with pixels sized 1.8 µm/px in XY and with 4 µm Z-steps (3.6x, 0.2 NA objective; ~4.5 µm tall PSF). With rapid 4-color acquisition (488, 561, 642, & 785 laser lines) of your embedded samples, we can precisely overlay multiplexed immunofluorescence signals and fluorescent protein expression patterns alike.

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Microscopy Consulting

Price on request

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

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Immunohistochemistry (IHC)

Price on request

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

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Immunofluorescence

Price on request

Please refer above to our service offering "Immunostaining Experimental Design" and "Light Sheet Microscopy" for more details.

Please refer above to our service offering "Immunostaining Experimental Design" and "Light Sheet Microscopy" for more details.

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Digital Microscopy

Price on request

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

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Fluorescence-Based Microscopy

Price on request

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

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Histological Staining

Price on request

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

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3D Immunostaining

Price on request

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

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Histology and Pathology Services

Price on request

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

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Imaging Technology Platforms

Price on request

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

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Preclinical Study Design

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Request a quote for more information about this service.

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Histomorphological Analysis

Price on request

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

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Tissue Analysis

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Request a quote for more information about this service.

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Preclinical Histology

Price on request

Please refer above to our service offering "Immunostaining Experimental Design" for more details

Please refer above to our service offering "Immunostaining Experimental Design" for more details

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3D Vasculature Imaging

Price on request

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

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Animal Whole Organ Imaging

Price on request

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

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Antibody Validation

Price on request

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

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Immunostaining

Price on request

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

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Protein Expression Visualization

Price on request

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

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Animal Models and Studies

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Request a quote for more information about this service.

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Clinical and Anatomic Pathology

Price on request

Please refer above to our service offering "Immunostaining Experimental Design" for more details

Please refer above to our service offering "Immunostaining Experimental Design" for more details

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Clinical Research

Price on request
Request a quote for more information about this service.

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Optical Microscopy

Price on request

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

Please refer above to our service offering "Light Sheet MIcroscopy" for more details.

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Molecular Phenotyping

Price on request

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

Please refer above to our service offering "Immunostaining Experimental Design" for more details.

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Biospecimens

Price on request

Biospecimens Services

Biospecimens Services

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Cells and Tissues

Price on request

Cells and Tissues Services

Cells and Tissues Services

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Whole-Organ Tissue Processing

Price on request

Tissue Processing Pipeline

Whole-organ tissue processing is a specialized and time-intensive process that affords researchers a new and unique perspective on anatomical analyses. At LifeCanvas Technologies, we help you reach your research goals by providing an integrated tissue-processing service that is built upon our... Show more »

Tissue Processing Pipeline

Whole-organ tissue processing is a specialized and time-intensive process that affords researchers a new and unique perspective on anatomical analyses. At LifeCanvas Technologies, we help you reach your research goals by providing an integrated tissue-processing service that is built upon our end-to-end pipeline of technology solutions.

Utilizing our proprietary devices and reagents, we carry out: (a) whole-organ tissue preservation (SHIELD), to protect endogenous fluorescence and preserve tissue antigenicity and architecture; (b) whole-organ delipidation / clearing (SmartClear 2 Pro), to remove light-scattering membrane lipids to enhance penetration of light and exogenous molecular probes; (c) whole-organ labeling (SmartLabel), to enable visualization of proteins and the structures they identify deep within the organ; and, (d) light-sheet microscopy (SmartSPIM), for fast acquisition of high-resolution images.

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Tissue Delipidation

Price on request

Delipidating tissue to enable intact-sample labeling and imaging involves exposing samples to harsh treatments like high temperatures and pH changes. If left unchecked, this can cause damage to proteins and overall tissue structure, leading to incomplete or inaccurate image datasets. Therefore, to preserve the sample’s endogenous... Show more »

Delipidating tissue to enable intact-sample labeling and imaging involves exposing samples to harsh treatments like high temperatures and pH changes. If left unchecked, this can cause damage to proteins and overall tissue structure, leading to incomplete or inaccurate image datasets. Therefore, to preserve the sample’s endogenous fluorescence and antigenicity along with tissue architecture, we use a novel tissue preservation technique called SHIELD that forms intramolecular bonds using polyfunctional, flexible epoxides to stabilize tissue architecture.

After SHIELD preservation, the tissue can be delipidated and either refractive index-matched for imaging or subjected to active immunohistochemical labeling. Notably, SHIELD-preserved tissues are well-suited for antibody multiplexing, i.e., iterative staining and de-staining, without loss of tissue antigenicity, to build up a rich picture of protein expression over repeated rounds of imaging.

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Luke Bogart

Director of Research
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Rhie Chung

Operations Manager
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Aparna Nambiar

Lab Assistant
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SHIELD - LifeCanvas Tech 2018.pdf

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SmartClear_iiPro_One-Sheet_2018_Nov. .pdf

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SmartLabel_One-Sheet_2018_Nov..pdf

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SmartSPIM_One-Sheet_2018_Nov..pdf

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