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LI-COR Biosciences

Lincoln, Nebraska, US

Thank you for choosing LI-COR Biosciences for your custom service needs. LI-COR Biosciences is a global leader in the design, manufacture, and marketing of high quality, innovative instruments, software, reagents, and integrated systems for biotechnology and drug discovery.

We have over 25 years of experience and expertise in near-infrared fluorescence reagents, instrumentation and applications. You can have complete confidence in the custom solutions provided.

Primary research areas include:
-- Probe design, validation, and manufacturing
-- Bioconjugation
-- Western blot analysis
-- Cell-based assays
-- Tissue section imaging
-- Antibody validation and screening
-- Molecular imaging
-- in vivo Imaging

During your project, a team of dedicated scientists will collaborate with you to provide:
-- A preliminary project consultation
-- Project progress updates
-- A complete project report

The services available are listed below.

Certification: ISO 9001:2015

LI-COR Biosciences has not listed any services.

NIR Digital Imaging
Price on request
Request a quote for more information about this service.
ICH Stability Testing
Price on request
Request a quote for more information about this service.
Analytical Method Development
Price on request

LI-COR provides analytical method development and validation services to meet your research and development needs. Whether you need to fully characterize compounds or perform routine analysis such as QC testing or an ICH stability study, our facilities are equipped and qualified to meet your project needs.

Our HPLC units are... Show more »

LI-COR provides analytical method development and validation services to meet your research and development needs. Whether you need to fully characterize compounds or perform routine analysis such as QC testing or an ICH stability study, our facilities are equipped and qualified to meet your project needs.

Our HPLC units are equipped with both UV and MS detectors (Agilent) and we also have a Thermo LTQ XL Linear Ion Trap mass spectrometer on site. Our scientists will work with you to provide quantum yield and extinction coefficient determinations of your compounds. We provide consultation services to determine the best options for sample analysis (e.g., HPLC, GC, NMR, Mass Spec, fluorescence spectroscopy, IR, water analysis using the Karl Fischer method) and compound stability studies in our qualified stability chambers.

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UV/Vis
FT-IR
pH
LC/MS
fluorescence spectroscopy
Analytical Chemistry Services
Price on request

LI-COR provides analytical method development and validation services to meet your research and development needs. Whether you need to fully characterize compounds or perform routine analysis such as QC testing or an ICH stability study, our facilities are equipped and qualified to meet your project needs.

Our HPLC units are... Show more »

LI-COR provides analytical method development and validation services to meet your research and development needs. Whether you need to fully characterize compounds or perform routine analysis such as QC testing or an ICH stability study, our facilities are equipped and qualified to meet your project needs.

Our HPLC units are equipped with both UV and MS detectors (Agilent) and we also have a Thermo LTQ XL Linear Ion Trap mass spectrometer on site. Our scientists will work with you to provide quantum yield and extinction coefficient determinations of your compounds. We provide consultation services to determine the best options for sample analysis (e.g., HPLC, GC, NMR, Mass Spec, fluorescence spectroscopy, IR, water analysis using the Karl Fischer method) and compound stability studies in our qualified stability chambers.

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NMR
HPLC
purity
UV/Vis
Reversed Phased Chromotography
FLASH Chromatography
Quantum yield
Extinction Coefficient
ICH Stability Studies
Reverse Phase Purification
water content
LC/MS
GC
Clinical Trials Supply Manufacturing
Price on request

Pharmaceutical manufacturing is resource-intensive. Pre-clinical and clinical development requires efficient transfer of manufacturing and testing protocols from lab-scale to larger-scale, as well as compliance with FDA and ICH regulatory expectations. You will need access to well-equipped facilities, trained personnel, and... Show more »

Pharmaceutical manufacturing is resource-intensive. Pre-clinical and clinical development requires efficient transfer of manufacturing and testing protocols from lab-scale to larger-scale, as well as compliance with FDA and ICH regulatory expectations. You will need access to well-equipped facilities, trained personnel, and rigorous SOPs and quality standards.

To meet your manufacturing needs, LI-COR provides integrated manufacturing services. You can consult with our experts for manufacturing of your dyes under cGMP for early-phase clinical trials. We also provide non-cGMP and custom manufacturing for GLP toxicology and other pre-clinical studies.

Manufacturing Solutions:
• Dye derivatives such as carboxylate, NHS ester, and maleimide
• IRDye 800CW, ICG derivatives, visible and near-infrared dyes, quenchers (QC-1), fluorescent probes
• API
• Small- or large-scale projects (milligram to kilograms)
• cGMP – early phase clinical trials
• Custom pack sizes

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Cancer
Drug Discovery
Ophthalmology
API
Toxicology
IRDye infrared dyes
IRDye 800CW
cGMP
non-cGMP
cGMP for early phase clinical trials
small scale
large scale
ICG
IRDye QC-1
activatable probes
dye-conjugation
dual-labeled probes
IRDye 700DX
ICG conjugation
Pharmaceutical Manufacturing
Price on request

Pharmaceutical manufacturing is resource-intensive. Pre-clinical and clinical development requires efficient transfer of manufacturing and testing protocols from lab-scale to larger-scale, as well as compliance with FDA and ICH regulatory expectations. You will need access to well-equipped facilities, trained personnel, and... Show more »

Pharmaceutical manufacturing is resource-intensive. Pre-clinical and clinical development requires efficient transfer of manufacturing and testing protocols from lab-scale to larger-scale, as well as compliance with FDA and ICH regulatory expectations. You will need access to well-equipped facilities, trained personnel, and rigorous SOPs and quality standards.

To meet your manufacturing needs, LI-COR provides integrated manufacturing services. You can consult with our experts for manufacturing of your dyes under cGMP for early-phase clinical trials. We also provide non-cGMP and custom manufacturing for GLP toxicology and other pre-clinical studies.

Manufacturing Solutions:
• API
• Dye derivatives such as carboxylate, NHS ester, and maleimide
• IRDye 800CW, ICG derivatives, visible and near-infrared dyes, quenchers (QC-1), fluorescent probes
• Small- or large-scale projects (milligram to kilograms)
• cGMP – early phase clinical trials
• Custom pack sizes

« Show less
Drug Discovery
Ophthalmology
API
Toxicology
IRDye infrared dyes
IRDye 800CW
cGMP
non-cGMP
cGMP for early phase clinical trials
small scale
large scale
ICG
IRDye QC-1
activatable probes
dual-labeled probes
IRDye 700DX
dye conjugation
ICG conjugation
Custom Manufacturing
Price on request

Manufacturing is resource-intensive. Pre-clinical and clinical development requires efficient transfer of protocols from lab-scale to larger-scale. You will need access to well-equipped facilities, trained personnel, and rigorous SOPs and quality standards. To meet your manufacturing needs, LI-COR provides integrated... Show more »

Manufacturing is resource-intensive. Pre-clinical and clinical development requires efficient transfer of protocols from lab-scale to larger-scale. You will need access to well-equipped facilities, trained personnel, and rigorous SOPs and quality standards. To meet your manufacturing needs, LI-COR provides integrated manufacturing services. You can consult with our experts for manufacturing of your dyes under cGMP for early-phase clinical trials. We also provide non-cGMP and custom manufacturing for GLP toxicology and other pre-clinical studies.

Manufacturing Solutions:
• API
• Dye derivatives such as carboxylate, NHS ester, and maleimide
• IRDye 800CW, ICG derivatives, visible and near-infrared dyes, quenchers (QC-1), fluorescent probes
• Small- or large-scale projects (milligram to kilograms)
• cGMP – early phase clinical trials
• Custom pack sizes

« Show less
Drug Discovery
Ophthalmology
API
Toxicology
IRDye infrared dyes
IRDye 800CW
cGMP
non-cGMP
cGMP for early phase clinical trials
small scale
large scale
ICG
IRDye QC-1
activatable probes
dual-labeled probes
IRDye 700DX
dye conjugation
ICG conjugation
Antibody Validation
Price on request

Antibody validation is an important step to ensure reproducible data from Western blots, cell based assays applications. The specificity and selectivity of the antibody must be confirmed. Accuracy in Western blot detection and cell based assays depends on many things, including proper antibody validation. LI-COR can assist in... Show more »

Antibody validation is an important step to ensure reproducible data from Western blots, cell based assays applications. The specificity and selectivity of the antibody must be confirmed. Accuracy in Western blot detection and cell based assays depends on many things, including proper antibody validation. LI-COR can assist in confirming your antibody recognizes the correct protein target before you begin your experiment.

For Antibody Validation:
• Send your antibody and samples to LI-COR
o For Antibody validation – include positive and negative control (e.g. recombinant protein and knock-out)
• LI-COR runs Western blot or cell-based assays.
• LI-COR delivers the data

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LI-COR Odyssey
Odyssey CLx
IRDye secondary antibodies
antibody screening
antibody validation
MPX Blotting System
hybridoma screening
Western Blot
Price on request

LI-COR offers three levels of Western blot service: 1) Imaging Only; 2) Advanced Imaging (Reagents and Near-infrared Imaging); and 3) Full-Service (Processing and Chemiluminescent or Near-infrared Fluorescent Imaging).

Your project will begin with a discussion of requirements, reagents, and detection methods. Both... Show more »

LI-COR offers three levels of Western blot service: 1) Imaging Only; 2) Advanced Imaging (Reagents and Near-infrared Imaging); and 3) Full-Service (Processing and Chemiluminescent or Near-infrared Fluorescent Imaging).

Your project will begin with a discussion of requirements, reagents, and detection methods. Both chemiluminescent and near-infrared fluorescent detection methods are available
Imaging Only Service is designed for researchers who currently have the necessary reagents and time for preparing a Western blot, but do not have the means to image the blot.

For chemiluminescent imaging:
• Submit a dry blot to LI-COR
• LI-COR incubates with an HRP-conjugated secondary antibody
• LI-COR applies a high sensitivity substrate
• LI-COR images on the Odyssey® Fc Imager

For near-infrared fluorescent imaging:
• Prepare your blot on nitrocellulose or low fluorescence PVDF (i.e. Immobilon®-FL) membranes*
• Incubate with IRDye® secondary antibodies, or antibodies labeled with fluorophores in the 700 nm to 800 nm range
• Submit a dry blot to LI-COR
• LI-COR images on the Odyssey CLx Infrared Imaging System

Your completed project will include image files for analysis in the following formats: jpeg, tiff, and Image Studio™ files. Image Studio files can be viewed and analyzed using Image Studio Lite (a free download), or the full version of Image Studio Software.
Note: analysis not provided.
Pricing:
Near-infrared fluorescent imaging
• 1-10 blots** $350. Each blot after 10 is an additional $25 / blot
Chemiluminescent imaging:
• 1-10 blots** $450. Each blot after 10 is an additional $45 / blot
*These membranes are preferred for their reduced background when imaging in the near-infrared wavelengths.
**Minimum order of 10 blots per imaging method

Advanced Imaging Service is designed for researchers who are interested in performing near-infrared Western blots, but lack the necessary reagents or instrumentation. LI-COR supports near-infrared imaging in the 700 nm to 800 nm range, and recommends the use of IRDye® secondary antibodies for this type of processing. The reagent kit provided will include membrane, blocking buffer, and IRDye® secondary antibodies.

For processing and imaging:
• LI-COR sends you optimal reagents for ten near-infrared fluorescent Western blots
• Prepare your blots using these reagents
• Submit dry blots to LI-COR
• LI-COR images the blots on the Odyssey® CLx Infrared Imager

When your project is complete you will receive images for analysis in the following formats: jpeg, tiff, and Image Studio™ files. Image Studio files can be viewed and analyzed using Image Studio Lite (a free download), or the full version of Image Studio Software.
Note: analysis not provided.
Pricing: 10 blots* at $65 / blot --- $650
*Minimum order of 10 blots

Full-Service Western blotting is designed for researchers who are interested in performing Western blots but lack the necessary reagents, instrumentation or time. The service is also useful if you need to troubleshoot. LI-COR scientists will perform the entire Western blotting procedure from start to finish.

For full-service Western blotting:
• Send your sample and primary antibody to LI-COR
• LI-COR generates an optimized protocol
• LI-COR performs the experiments

When your project is complete you will receive an optimized protocol and image files for analysis in the following formats: jpeg, tiff, and Image Studio™ files. Image Studio files can be viewed and analyzed using Image Studio Lite (a free download) or the full version of Image Studio Software.
Note: analysis not provided.
Pricing: Inquire

« Show less
Cancer
western blot
Alzheimers Disease
Western blotting
Parkinson's disease (PD)
LI-COR Odyssey
Odyssey CLx
Odyssey Fc
image studio
image studio lite
western blot normalization
western blot optimization
near0infrared western blot
quantitative western blot
protocol development
Autoimmune disease
Direct Antibody Labeling
Price on request

This service is designed for researchers requiring assistance in labeling antibodies. Your project will begin with a discussion of requirements, and the best method for labeling your antibody. LI-COR scientists who specialize in labeling can provide advice for getting the best end product for your application.

For labeling... Show more »

This service is designed for researchers requiring assistance in labeling antibodies. Your project will begin with a discussion of requirements, and the best method for labeling your antibody. LI-COR scientists who specialize in labeling can provide advice for getting the best end product for your application.

For labeling reactions 1mg or larger:
• Send your antibody to LI-COR
• LI-COR labels the antibody
• Receive the final product in a format of your choice

Protein concentration and/or dye/protein ratio information are sent with each order. Stabilizers can be added and additional supporting information is available on request.

Pricing:
Starting Price per Labeling Reaction
• 1-2 mg $500
• 2-5 mg $1,250
• 5-10 mg $1,650
• 10 mg Inquire

« Show less
Drug Discovery
fluorophores
protein labeling
protein conjugation
IRDye infrared dyes
IRDye 800CW
IRDye 680RD
ICG
IRDye QC-1
IRDye 700DX
antibody labeling
antibody conjugation
peptide labeling
peptide conjugation
Antibody Screening
Price on request

LI-COR can screen multiple samples and multiple targets all on the same Western blot. No cutting. No stripping. No cross-contamination. Up to 48 unique antibodies can be screened against a single sample. Or multiple samples can be used to screen a smaller number of antibodies or antibody dilutions. Perfect for hybridoma... Show more »

LI-COR can screen multiple samples and multiple targets all on the same Western blot. No cutting. No stripping. No cross-contamination. Up to 48 unique antibodies can be screened against a single sample. Or multiple samples can be used to screen a smaller number of antibodies or antibody dilutions. Perfect for hybridoma screening. Subsequent rounds of screening will narrow your list to provide antibodies with the highest specificity.

For Antibody Screening:
• Send your antibodies and samples to LI-COR
• include positive and negative controls (e.g. recombinant protein and knock-out)
• LI-COR runs Western blots to select the best performing antibody
• LI-COR delivers the data

« Show less
LI-COR Odyssey
Odyssey CLx
IRDye secondary antibodies
multiplex Western blot
antibody screening
antibody validation
Cell-Based Assays
Price on request

Cell-based assays are quantitative immunofluorescence assays performed in microplates. LI-COR pioneered the In-Cell Western and On-Cell Western assays.

In-Cell Western™ Assays
The In-Cell Western (ICW) Assay is a quantitative immunofluorescence assay performed in microplates (96- or 384-well format). It combines the... Show more »

Cell-based assays are quantitative immunofluorescence assays performed in microplates. LI-COR pioneered the In-Cell Western and On-Cell Western assays.

In-Cell Western™ Assays
The In-Cell Western (ICW) Assay is a quantitative immunofluorescence assay performed in microplates (96- or 384-well format). It combines the specificity of Western blotting with the reproducibility and throughput of ELISA. With In-Cell Western™ assays you can:

• Detect proteins in fixed cultured cells using target-specific primary antibodies and IRDye® fluorescent secondary antibodies
• Quantify two targets at 700 nm and 800 nm, using spectrally-distinct infrared dyes
• Quickly, accurately measure relative protein levels in many samples
• Detect proteins in situ in a relevant cellular context

ICW assays are used for analysis of:
• Protein phosphorylation (effects of drug compounds on signaling pathways; timing/kinetics of signal transduction; IC50 determination)
• GPCR activation
• Tau protein accumulation and inhibition
• Gene expression levels
• Viral titer/load
• Apoptosis: Caspase-3 activation
• Cell surface proteins and receptor internalization
• RNAi library screens
• Cell proliferation assays

For In-Cell Western Assays:
• Consult with LI-COR to determine appropriate cell line, drug treatment, inhibitor/stimulate to use.
• LI-COR performs the assay.
• LI-COR delivers the data

On-Cell Western Assay
The On-Cell Western Assay, a variation of the In-Cell Western™ Assay, is a cell-based assay that enables quantitative monitoring of cell surface protein expression. The On-Cell Western offers the ability to:
• Detect and quantify target proteins localized to the cell surface
• Quantify ligand binding to cell surface receptors
• Monitor receptor internalization and recycling by detecting loss and re-appearance at the cell surface
• Perform and detect cell surface biotinylation assays
• Evaluate the effects of mutations, drugs, and other treatments on protein trafficking
• Analyze many samples quickly and quantitatively

For On-Cell Western Assays:
• Consult with LI-COR to determine appropriate cell line, drug treatment, inhibitor/stimulate.
• LI-COR labels antibody or small molecule
• LI-COR performs binding and/or competition assay to determine specificity.
• LI-COR delivers the data

« Show less
Cell-based assays
LI-COR Odyssey
Odyssey CLx
image studio
image studio lite
IRDye secondary antibodies
in-cell western assay
on-cell western assay
in-cell ELISA
signal transduction pathway analysis
determination of IC50
Odyssey CLx
LI-COR Odyssey
Optical Probe Development and Validation
Price on request

Validation is a meticulous venture, but you can maximize your chances of success with a streamlined approach. Start with assessing binding affinities and specificities of your probe in cells. Then move your ‘hits’ to animals for tracking biodistribution, specificity, and clearance of the probe. Excise the tissues and look at... Show more »

Validation is a meticulous venture, but you can maximize your chances of success with a streamlined approach. Start with assessing binding affinities and specificities of your probe in cells. Then move your ‘hits’ to animals for tracking biodistribution, specificity, and clearance of the probe. Excise the tissues and look at specificity and localization at the organ and tissue levels.

We can help with microscopic analysis of probe uptake and specificity in tissues and cells using near-infrared microscope. Ask us about assay development, reagent validation, imaging for optical probe development projects.

In vitro Probe Validation
Now that you have an approach to measure in vitro activity, you need a sensitive detection system that can accurately help you quantitate minute differences in molecular activity. That is where Odyssey CLx comes in. With its industry-leading dynamic range of detection, we can accurately quantitate and normalize minute changes with confidence.

For in vitro Probe Validation:
• Send your antibody, peptide or small molecule to LI-COR.
• Peptides can be sourced directly through LI-COR to facilitate your project.
• LI-COR performs the conjugation, purification and analysis of the final product
• LI-COR performs in vitro binding and specificity studies in desired cell line.
• LI-COR delivers the data

In vivo Probe Validation
Pearl Trilogy – sensitive detection of in vivo activity; no camera adjustments needed
Extend the advantages of a wide dynamic range and sensitive NIR detection to your in vivo animal studies. Using Pearl Trilogy, animal tissue is rendered virtually transparent which greatly helps monitor probe clearance and biodistribution. You need not adjust camera or exposure settings between scans.

For in vivo Probe Validation:
• Consult with LI-COR regarding mouse model and study parameters.
• Send your antibody, peptide or small molecule to LI-COR.
• Peptides can be sourced directly through LI-COR to facilitate your project.
• LI-COR performs the conjugation, purification and analysis of the final product
• LI-COR performs in vivo probe validation studies
• LI-COR delivers the data

Ex vivo Probe Validation
Odyssey CLx – Macroscopic analysis of tissue sections
Pearl Trilogy – Whole organ imaging
Now that you have assessed probe performance in animals, you can excise organs and tissue sections for uptake, localization, and biodistribution. The Odyssey imager has a 21 um resolution that allows efficient macroscopic analysis of tissue sections, up to 30 slides at a time. The large scan area also lets you assess probe activity in organs.

For ex vivo Probe Validation:
• Consult with LI-COR regarding mouse model and study parameters.
• Send your antibody, peptide or small molecule to LI-COR.
• Peptides can be sourced directly through LI-COR to facilitate your project.
• LI-COR performs the conjugation, purification and analysis of the final product
• LI-COR performs ex vivo probe validation studies
• LI-COR delivers the data

« Show less
Drug Discovery
In vivo assays
in vitro assays
biodistribution
LI-COR Odyssey
Odyssey CLx
image studio
image studio lite
Pearl Trilogy
LI-COR Pearl
probe validation
ex vivo probe validation
in vivo probe validation
in vitor probe validation
clearance
whole animal imaging
tissue section imaging
Protein Conjugation
Price on request

This service is designed for researchers requiring assistance in labeling proteins. Your project will begin with a discussion of requirements, and the best method for labeling your protein. LI-COR scientists who specialize in labeling can provide advice for getting the best end product for your application.
For labeling... Show more »

This service is designed for researchers requiring assistance in labeling proteins. Your project will begin with a discussion of requirements, and the best method for labeling your protein. LI-COR scientists who specialize in labeling can provide advice for getting the best end product for your application.
For labeling reactions 1mg or larger:
• Send your antibody to LI-COR
• LI-COR labels the antibody
• Receive the final product in a format of your choice

Protein concentration and dye/protein ratio information are sent with each order. Stabilizers can be added and additional supporting information is available on request.

Pricing:
Starting Price per Labeling Reaction
• 1-2 mg $500
• 2-5 mg $1,250
• 5-10 mg $1,650
• 10 mg Inquire

« Show less
Cancer
Drug Discovery
fluorophores
protein labeling
protein conjugation
dual labeling
IRDye infrared dyes
IRDye 800CW
IRDye 680RD
ICG
activatable probes
IRDye 700DX
antibody labeling
antibody conjugation
peptide labeling
peptide conjugation
Tissue Section Imaging
Price on request

This service is designed for researchers who are interested in performing tissue section imaging, but lack the necessary instrumentation or time.

Your project will begin with a discussion of requirements, reagents and instrumentation. Near-infrared imaging is available at 700 nm and 800 nm with a resolution of 21 µm on the... Show more »

This service is designed for researchers who are interested in performing tissue section imaging, but lack the necessary instrumentation or time.

Your project will begin with a discussion of requirements, reagents and instrumentation. Near-infrared imaging is available at 700 nm and 800 nm with a resolution of 21 µm on the Odyssey® CLx Imaging System. Additional fluorescent detection channels and resolutions are available using an Olympus IX81 microscope.

For tissue section imaging on the Odyssey CLx Imager or Olympus microscope:
• Provide your tissue sections* treated with stains or fluorophores
• LI-COR images tissue sections on the Odyssey CLx Imager or Olympus microscope

When your project is complete you will receive image files for analysis in the following formats: tiff.

Note: analysis not provided.

Pricing:
Infrared Imaging on the Odyssey CLx Imaging System: 10 slides** at $25 / slide --- $250

Imaging on the Olympus IX81 microscope: Inquire

*No human samples.
**Minimum order of 10 slides

« Show less
LI-COR Odyssey
Odyssey CLx
image studio
image studio lite
IRDye infrared dyes
Pearl Trilogy
LI-COR Pearl
tissue section imaging
IRDye 800CW
IRDye 680RD
macroscopic tissue imaging
whole organ imaging
IRDye secondary antibodies
In-Cell Western Assay
Price on request

The In-Cell Western (ICW) Assay is a quantitative immunofluorescence assay performed in microplates (96- or 384-well format). It combines the specificity of Western blotting with the reproducibility and throughput of ELISA. With In-Cell Western™ assays you can:

• Detect proteins in fixed cultured cells using target-specific... Show more »

The In-Cell Western (ICW) Assay is a quantitative immunofluorescence assay performed in microplates (96- or 384-well format). It combines the specificity of Western blotting with the reproducibility and throughput of ELISA. With In-Cell Western™ assays you can:

• Detect proteins in fixed cultured cells using target-specific primary antibodies and IRDye® fluorescent secondary antibodies
• Quantify two targets at 700 nm and 800 nm, using spectrally-distinct infrared dyes
• Quickly, accurately measure relative protein levels in many samples
• Detect proteins in situ in a relevant cellular context

ICW assays are used for analysis of:
• Protein phosphorylation (effects of drug compounds on signaling pathways; timing/kinetics of signal transduction; IC50 determination)
• GPCR activation
• Tau protein accumulation and inhibition
• Gene expression levels
• Viral titer/load
• Apoptosis: Caspase-3 activation
• Cell surface proteins and receptor internalization
• RNAi library screens
• Cell proliferation assays

For In-Cell Western Assays:
• Consult with LI-COR to determine appropriate cell line, drug treatment, inhibitor/stimulate to use.
• LI-COR performs the assay.
• LI-COR delivers the data

« Show less
Cell-based assays
LI-COR Odyssey
in-cell western
plate-based assays
Odyssey CLx
Odyssey Fc
image studio
image studio lite
Biodistribution Studies
Price on request

Ex vivo Biodistribution Studies:

Biodistribution and clearance assessments track the movement of a probe to understand its uptake, localization, distribution, and elimination within the animal. IRDye® 800CW and IRDye 680RD provide excellent performance for ex vivo biodistribution imaging. Biodistribution studies can... Show more »

Ex vivo Biodistribution Studies:

Biodistribution and clearance assessments track the movement of a probe to understand its uptake, localization, distribution, and elimination within the animal. IRDye® 800CW and IRDye 680RD provide excellent performance for ex vivo biodistribution imaging. Biodistribution studies can identify regions and time of specific and non-specific retention of the probe in organs and tissues.

For ex vivo biodistribution analysis, tissues excised from the animal (tumors, organs) are assessed for relative accumulation of the probe. Macroscopic analysis of tissue sections and whole organ imaging allow a quick screening overview, while microscopic analysis provides additional detail of cellular localization of the probe.

The Pearl® Trilogy Imaging System can perform rapid time-lapse and longitudinal in vivo near-infrared imaging without the need to manually adjust camera settings between scans. For ex vivo analysis, the Organ Tray Base and disposable organ trays provide a non-heated, flat surface for imaging on the Pearl Trilogy system.

In vivo Biodistribution and Clearance Studies:

Biodistribution and clearance assessments track the movement of a probe to understand its uptake, localization, distribution, and elimination within the animal. Biodistribution studies can identify regions and time of specific and non-specific retention of the probe in organs and tissues.

In vivo biodistribution analysis in live animals tracks the localization of the probe and reveals if it binds to the intended target, as well as its elimination route. Non-specific interactions of the probe are very important and should not be overlooked.

In vivo clearance studies monitor the elimination of the optical probe over time, in live animals. Knowledge of probe elimination is important to optimize both imaging time and dose, as well as to identify any specific or non-specific retention, which may impact imaging results.
IRDye® 800CW and IRDye 680RD provide excellent performance for in vivo biodistribution imaging.

The Pearl® Trilogy Imaging System can perform rapid time-lapse and longitudinal in vivo NIR imaging without the need to manually adjust camera settings between scans.

« Show less
Cancer
Drug Discovery
Alzheimers Disease
biodistribution
Parkinson's disease (PD)
image studio
image studio lite
Toxicology
IRDye infrared dyes
Pearl Trilogy
LI-COR Pearl
clearance
in vivo imaging
organ imaging
Pearl Trilogy
LI-COR Pearl
IRDye 800CW
IRDye 680RD
ICG
small animal imaging
Chemical Crosslinking/Bioconjugation
Price on request

IRDye Bioconjugation

To meet your unique probe design needs, LI-COR offers several near-infrared dye fluorophores (IRDye®) for high-sensitivity imaging. Light absorption by tissue and cellular components and autofluorescence from those is low in the near-infrared imaging window, so you get optimal detection signals for... Show more »

IRDye Bioconjugation

To meet your unique probe design needs, LI-COR offers several near-infrared dye fluorophores (IRDye®) for high-sensitivity imaging. Light absorption by tissue and cellular components and autofluorescence from those is low in the near-infrared imaging window, so you get optimal detection signals for tracking assessing biochemical changes in cell-based assays or tracking biodistribution of dye-conjugated molecules in animals.
• High signal-to-noise ratios
• Versatile for in vitro and in vivo use
• History of investigational use in clinical trials

IRDye 800CW and IRDye 700DX dye-conjugated probes and agents have propelled more than 20 Phase I and II clinical trials.

For IRDye Bioconjugation:
• Send your antibody, peptide or small molecule to LI-COR.
• Peptides can be sourced directly through LI-COR to facilitate your project.
• LI-COR performs the conjugation, purification and analysis of the final product
• LI-COR delivers the product

« Show less
Drug Discovery
In vivo bioassays
In vitro bioassays
In vivo assays
in vitro assays
biodistribution
IRDye infrared dyes
in vivo imaging
IRDye 800CW
bioconjugation
high-sensitivity imaging
IRDye 680RD
On-Cell Western Assay
Price on request

The On-Cell Western Assay, a variation of the In-Cell Western™ Assay, is a cell-based assay that enables quantitative monitoring of cell surface protein expression. The On-Cell Western offers the ability to:
• Detect and quantify target proteins localized to the cell surface
• Quantify ligand binding to cell surface... Show more »

The On-Cell Western Assay, a variation of the In-Cell Western™ Assay, is a cell-based assay that enables quantitative monitoring of cell surface protein expression. The On-Cell Western offers the ability to:
• Detect and quantify target proteins localized to the cell surface
• Quantify ligand binding to cell surface receptors
• Monitor receptor internalization and recycling by detecting loss and re-appearance at the cell surface
• Perform and detect cell surface biotinylation assays
• Evaluate the effects of mutations, drugs, and other treatments on protein trafficking
• Analyze many samples quickly and quantitatively

For On-Cell Western Assays:
• Consult with LI-COR to determine appropriate cell line, drug treatment, inhibitor/stimulate.
• LI-COR labels antibody or small molecule
• LI-COR performs binding and/or competition assay to determine specificity.
• LI-COR delivers the data

« Show less
Cancer
Drug Discovery
Cell-based assays
High-throughput data analysis
LI-COR Odyssey
Odyssey CLx
image studio
image studio lite
Toxicology
IRDye secondary antibodies
in-cell western assay
on-cell western
icw
plate-based assay
Synthetic Chemistry
Price on request

Taking Compounds from Structures to Yield.

Get on-demand synthesis of organic compounds; starting materials, intermediates, or derivatives, from milligram to multigram amounts. LI-COR Custom Services can also help you optimize syntheses and chemical modification reactions for small molecule compounds and dyes.

Need custom... Show more »

Taking Compounds from Structures to Yield.

Get on-demand synthesis of organic compounds; starting materials, intermediates, or derivatives, from milligram to multigram amounts. LI-COR Custom Services can also help you optimize syntheses and chemical modification reactions for small molecule compounds and dyes.

Need custom synthesis for smaller-scale studies? Ask us about custom manufacturing services for research use only (RUO), and non-cGMP manufacturing for preclinical and toxicity studies. Our complete manufacturing solutions include analytical method development, manufacturing protocols, and stability studies under International Council for Harmonisation (ICH) guidelines.

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Karl Fisher Analysis
Nuclear Magnetic Resonance (NMR) Spectroscopy
UV/Vis Spectrophotometry
Fourier Transform Infrared Spectroscopy (FTIR)
Gas Chromatography Mass Spectrometry (GC/MS)
High Performance Liquid Chromatography (HPLC) analysis
FLASH Chromatography
Quantum yield
Extinction Coefficient
ICH Stability Studies
Fourier Transform Infrared Spectroscopy (FTIR)
UV/Vis Spectrophotometry
FLASH Chromatography
Reverse Phase Purification
Organic Synthesis
Lyophilization Services
Dye Manufacturing
Price on request

Manufacturing is resource-intensive. Pre-clinical and clinical development requires efficient transfer of protocols from lab-scale to larger-scale. You will need access to well-equipped facilities, trained personnel, and rigorous SOPs and quality standards.

To meet your manufacturing needs, LI-COR provides integrated... Show more »

Manufacturing is resource-intensive. Pre-clinical and clinical development requires efficient transfer of protocols from lab-scale to larger-scale. You will need access to well-equipped facilities, trained personnel, and rigorous SOPs and quality standards.

To meet your manufacturing needs, LI-COR provides integrated manufacturing services. You can consult with our experts for manufacturing of your dyes under cGMP for early-phase clinical trials. We also provide non-cGMP and custom manufacturing for toxicity and pre-clinical studies.

Manufacturing Solutions:
• Dye derivatives such as carboxylate, NHS ester, and maleimide
• IRDye, ICG, visible dyes, quenchers (QC-1)
• Small- or large-scale projects (grams to kilograms)
• cGMP – early phase clinical trials
• non-cGMP – toxicity and preclinical
• Custom pack sizes

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Cancer
Drug Discovery
cGMP-compliant
Toxicology
IRDye infrared dyes
cGMP
non-cGMP
cGMP for early phase clinical trials
dye conjugation
dual-labeled probes
optical probes
Immunoprecipitation
Price on request

Immunoprecipitation (IP) is a valuable tool that utilizes antibody:antigen interactions to isolate a specific
target protein from thousands of proteins in a complex solution. The specificity of the reaction increases
the concentration of dilute proteins as high as 10,000-fold; a property that renders the technique useful... Show more »

Immunoprecipitation (IP) is a valuable tool that utilizes antibody:antigen interactions to isolate a specific
target protein from thousands of proteins in a complex solution. The specificity of the reaction increases
the concentration of dilute proteins as high as 10,000-fold; a property that renders the technique useful in
examination of rare proteins. With careful choice of antibodies, a co-IP can also be used to demonstrate
protein interaction partners*.
The typical workflow for an IP can be divided into four parts:
1. Incubation of the primary antibody with the sample (e.g., a cell lysate);
2. Capture of the antibody:antigen complex using a solid support such as Protein A agarose beads;
3. Separation of the proteins by SDS-PAGE;
4. Detection of the target protein by Western blot.

For this service:
• Send your sample and primary antibody to LI-COR
• LI-COR generates an optimized protocol
• LI-COR performs the experiments

When your project is complete you will receive an optimized protocol and image files for analysis in the following formats: jpeg, tiff, and Image Studio™ files. Image Studio files can be viewed and analyzed using Image Studio Lite (a free download) or the full version of Image Studio Software.
Note: analysis not provided.
Pricing: Inquire

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western blot
immunoprecipitation
Western blotting
LI-COR Odyssey
Odyssey CLx
Odyssey Fc
Odyssey CLx
LI-COR Odyssey
Odyssey Fc
2018-10-08 12:50:36 -1100

Net Promoter Score of 10 received for Tissue Section Imaging.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-09-17 12:31:12 -1100

Net Promoter Score of 10 received for Analytical Chemistry Services.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-05-03 04:46:25 -1100

Net Promoter Score of 9 received for Direct Antibody Labeling.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 10.
2018-04-12 07:51:17 -1100

Net Promoter Score of 7 received for Antibody Services.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 7.
2018-03-14 11:06:34 -1100

Net Promoter Score of 10 received for Chemical Crosslinking/Bioconjugation.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-12-18 10:27:39 -1100

Net Promoter Score of 10 received for Western Blot.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-12-11 05:44:46 -1100

Net Promoter Score of 10 received for Western Blot.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
August 23, 2017

Positive review received for Western Blot:

"Very knowledgeable about the methods they use; superior technical performance; and outstanding data reporting."

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