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LGC

Beverly, Massachusetts, US

LGC offers genomics solutions in the following areas:

  • Sample preparation and nucleic acid extraction
  • DNA sequencing
  • Genotyping

The company is unique in the genomics market place with respect to our position both as a laboratory services provider and a developer of proprietary chemistries and instrumentation to drive cost efficient genomics solutions.

We use what we sell and sell what we use

We understand how a genomics laboratory works, we have the largest genotyping lab in the world in the UK with labs in Berlin-Germany, Boston-USA and London-UK.

LGC has not listed any services.

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Genomic DNA Extraction
Price on request

The first step in any study investigating genetic differences in an organism of interest is the purification of DNA and the nucleic acid extraction teams at LGC provides DNA and RNA extraction services to meet the demands of any project.

Depending on project size, specific customer requirements and the biological background of... Show more »

The first step in any study investigating genetic differences in an organism of interest is the purification of DNA and the nucleic acid extraction teams at LGC provides DNA and RNA extraction services to meet the demands of any project.

Depending on project size, specific customer requirements and the biological background of the project, we can implement an extraction service solution using any one or combination of our different extraction technologies to provide and optimised fit-for-purpose solution. Our range of genomics services includes high quality DNA and RNA extraction solutions.

DNA extraction

We have developed extraction protocols from a wide range of starting materials and volumes which enable the delivery of off-the-shelf services for most applications or tailor-made solutions utilising our unique range of in-house technologies.

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Illumina Sequencing
Price on request

Next-generation sequencing (NGS) technologies have dramatically reduced the costs of sequencing and led to novel approaches in many fields of biology. LGC offers the complete range of NextGen sequencing services using a range of platforms and library preparation solutions. NGS services are delivered on:

*Illumina HiSeq 2000... Show more »

Next-generation sequencing (NGS) technologies have dramatically reduced the costs of sequencing and led to novel approaches in many fields of biology. LGC offers the complete range of NextGen sequencing services using a range of platforms and library preparation solutions. NGS services are delivered on:

Illumina HiSeq 2000 and MiSeq platforms.

Consultation and the delivery of optimised solutions that meet the specific needs of a particular project are the foundation of our NGS service offering. Examples of our expertise include the development of a proprietary method to sequence complete transcriptomes and we have developed significant experience in sequencing bacterial and fungal genomes as well as metagenomes from various habitats.

Example applications

  • De novo sequencing including gap closure
  • Re-sequencing of genomes
  • Transcriptome sequencing and analysis (of both eukaryotic and prokaryotic organisms)
  • Metagenome sequencing and analysis
  • Analysis of:
    • Amplicons (e.g. 16S rDNA)
    • Pools of tagged fosmids / BACs

Special customised applications

  • Targeted re-sequencing of enriched individual genomic regions of interest
    • Exomes
    • Customised chromosomal intervals
  • Sample multiplexing
  • Bioinformatics
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RNA Extraction
Price on request

The first step in any study investigating genetic differences in an organism of interest is the purification of DNA and the nucleic acid extraction teams at LGC provides DNA and RNA extraction services to meet the demands of any project.

Depending on project size, specific customer requirements and the biological background of... Show more »

The first step in any study investigating genetic differences in an organism of interest is the purification of DNA and the nucleic acid extraction teams at LGC provides DNA and RNA extraction services to meet the demands of any project.

Depending on project size, specific customer requirements and the biological background of the project, we can implement an extraction service solution using any one or combination of our different extraction technologies to provide and optimised fit-for-purpose solution. Our range of genomics services includes high quality DNA and RNA extraction solutions.

RNA extraction

Our RNA extraction services include the ability to prepare RNA from:

RNAlater® preserved material
PAXgene® samples
Frozen sample material.

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Whole Genome Amplification
Price on request

Whole Genome Amplification (WGA) is a PCR technique that is used to produce large quantities of DNA from a small amount of starting material. Unlike conventional PCR, WGA is aimed at amplifying the entire genome of an organism rather than a specific region. The resultant amplified genomic DNA can then be used in a range of... Show more »

Whole Genome Amplification (WGA) is a PCR technique that is used to produce large quantities of DNA from a small amount of starting material. Unlike conventional PCR, WGA is aimed at amplifying the entire genome of an organism rather than a specific region. The resultant amplified genomic DNA can then be used in a range of applications including KASP genotyping.

How do we do WGA?

Two main techniques exist for performing WGA:

  1. Primer extension pre-amplification using thermostable DNA polymerases (PEP-PCR)
  2. Amplification by strand displacement using Phi29 DNA polymerase.

We have evaluated both of these techniques to assess amplification efficiency, suitability of DNA for downstream applications, and cost effectiveness. We strongly favour the first technique, primer extension pre-amplification (PEP-PCR), and have developed a WGA protocol that is based on this method. This protocol employs the use of an oligonucleotide that binds throughout the genome to act as a primer, allowing replication of the genomic DNA by KlearTaq™ hot-start DNA polymerase.

We have developed a WGA kit that is available to purchase in a range of reaction sizes, and we also offer WGA as a service in our laboratories.

Requirements and performance of WGA

A minimum mass of 50 ng DNA is required per reaction and, if the DNA is of good quality, the product is usually amplified to a concentration of 500 - 1000 times that of the starting material. This resultant DNA should be sufficient to perform KASP genotyping on at least 1000 SNPs.

If the genomic DNA is from a species with a genome size considerably larger than that of human, the required starting mass of DNA will be greater. As a guideline, divide the genome size of your organism by the size of the human genome (3000 Mbp), and use the resulting number to multiply the amount of DNA required

e.g. Triticum aestivum (wheat): 16000 Mbp.
16000 Mbp / 3000 Mbp = 5.3

You will need 5.3 times as much wheat DNA per reaction = 50 ng x 5.3 = 265 ng.

In-house validated service

We offer WGA of your DNA samples in our service laboratory. We utilise the amplification power of the Hydrocycler™ 16 that enables amplification of a large number of samples overnight. WGA reactions are performed in 96-well PCR plates (Greiner), and plates are sealed with Thermal Bond (4titude) using a Kube™ thermal plate sealer. This technique for sealing reaction plates facilitates easy access to the completed WGA reactions as the Thermal Bond seals are peelable.

WGA DNA for KASP genotyping

Completed WGA reactions can be used as starting template for KASP genotyping projects in our service laboratory. The figures below are the KASP genotyping results for a project within which the mass of original starting DNA was very limited. The figure on the left illustrates KASP genotyping results from the original starting material; the data points have not formed tight clusters and many of the samples have not amplified. WGA was performed in-house using 30 ng of the original DNA. The resultant amplified DNA was genotyped and results are shown in the right hand figure; the data points have formed three distinct clusters and, following checks for concordance, were found to yield 100% accuracy. The WGA reactions yielded sufficient DNA for approximately 1000 SNPs to be genotyped using KASP.

Yields

Typically, from 30 - 50 ng of genomic DNA the WGA will yield enough DNA to perform SNP genotyping on at least 1000 SNPs. In practice if the starting DNA is good it will be in excess of this. This represents an amplification of approximately 500 - 1000 fold.

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Genotyping by PCR
Price on request

The KASP™ genotyping chemistry delivers accurate, flexible and cost-effective genotyping service solutions. Using our PCR-based KASP genotyping assay, in conjunction, with KASP Master mix, LGC is able to provide complete genotyping services to our customers. We run the largest genotyping laboratory of its kind in the world. We... Show more »

The KASP™ genotyping chemistry delivers accurate, flexible and cost-effective genotyping service solutions. Using our PCR-based KASP genotyping assay, in conjunction, with KASP Master mix, LGC is able to provide complete genotyping services to our customers. We run the largest genotyping laboratory of its kind in the world. We have a track record of delivering the highest quality data, delivering break-through cost savings across a huge range of project types, and working with hundreds of different species.

Genotyping and all-inclusive services

Service Express Genotyping Regular Genotyping All-inclusive services
Prices Starting at <£0.15 per datapoint Starting at <£0.10 per datapoint Starting at £0.95 per sample
Turnaround time (more info) 2 weeks 4 weeks 6 weeks

Faster bespoke turnaround times available on request. Please contact us for more information.

Our expertise

  • More than 20,000 individual genotyping projects
  • A billion data points generated
  • Experience hundreds of different species
  • Supporting life science researchers in diverse application areas including
    • Crop and livestock development
    • Healthcare
    • Early stage research and diagnostics applications
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SNP Discovery Sequencing
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Sanger Sequencing
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KASP Genotyping
Kompetitive Allele Specific PCR
Price on request

From $5.00 for 100 SNP markers including extraction.

From $5.00 for 100 SNP markers including extraction.

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Genotyping Services
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DNA Sequencing
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DNA Extraction and Purification
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Biology
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Next Generation Sequencing (NGS)
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DNA Services
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RNA Services
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DNA Amplification
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Biochemistry & Molecular Biology
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Nucleic Acid Services
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