Lena Biosciences develops, markets, and sells cutting-edge tools and services that enable the use of human cells in culture in a physiologically relevant setting. The proprietary multiwell-plate perfusion system, PerfusionPal, mimics interstitial delivery of nutrients and drugs to organoids, 3D cell cultures, and tissue explants for translatable results. Our SeedEZ scaffold enables cells to utilize both their cadherin (cell-cell) and integrin (cell-ECM) receptors that are vital for tissue-representative cell behavior, drug- and cell therapy outcomes. Lastly, our Blood Substitute facilitates superior interstitial delivery of all gases to cells, thus increasing cellular respiratory, and drug metabolism by at least an order of magnitude compared to cells in a dish.
Neuronal assays in 2D and 3D. Testing may also be performed in our cortical model comprising tri-culture of neurons, astrocytes and microglia. Cells may be further supported by our Blood Substitute that increases cellular respiratory metabolism several-fold and reduces cell death. Cultures can also be perfused in our proprietary PerfusionPal organ-on-a-chip-like system providing month-long support.
Our PerfusionPal platform enables testing of 12 statistically-independent organ cultures in one plate. The price of a plate is $350.
Organoids are formed using co-cultures of iPSCs and primary cells and cultured in our proprietary PerfusionPal organ-on-a-chip-like system.
The assays are run in our proprietary SeedEZ scaffold that enables both cadherin and integrin signaling in 3D. Commons assays include: CellTiter-Glo, alamarBlue, LDH release, etc. We routinely run assays with primary cells and iPSCs and have extensive experience with brain co-cultures, liver and cancer models such as breast, liver, lung cancer etc.
We are offering short-term and long-term DILI using primary liver cells with a log-fold increase in Phase I drug metabolism, and a multi-fold increase in cell respiratory metabolism.
Off-target toxicity is evaluated in our 12-well organ-on-a-chip-like system PerfusionPal. For in vitro cell therapy testing, the toxicity of activated immune cells is measured after co-culture with 3D human organ models or after incubation of 3D organ models in activated immune cell medium.
Cytotoxicity is offered in 2D and 3D, and in advanced, synthetically vascularized models supported by blood substitute and interstitial perfusion. Assays may be performed using a client's cells and standard assays (CellTiter-Glo or total LDH, kinetic LDH release etc.). For immune cell therapy toxicity, immune cells may be co-cultured with the organ models or the organ models exposed to activated immune cell medium to assess toxicity in vitro.
We perform CYP induction assays using primary human hepatocytes in 2D and 3D. Advanced, perfused liver models are also available. Such models provide log-fold increase in CYP450 activity relative to 2D.
We perform CYP inhibition assays using primary human hepatocytes in 2D and 3D. Advanced, perfused liver models are also available. Such models provide log-fold increase in CYP450 activity relative to 2D.
We can generate advanced 3D tissue models using co-cultures of primary cells or cell lines based on your specifications, or using cells provided by you. Models may be perfused (interstitial perfusion) and vascularized in our proprietary PerfusionPal system.
We are offering in vitro toxicity testing services in 2D and 3D.
We are offering Phase I drug metabolism services using perfused 3D cultures of primary human hepatocytes with a log-fold increase in CYP450 activity.
We create perfused 3D culture models using cells provided by a client.
We routinely culture in 2D and 3D, and generate organoid-like and perfused 3D cultures. Using our proprietary Blood Substitute and/or perfusion we can support tissue slice cultures longer.
We provide routine 2D and 3D iPSC cell cultures and co-cultures. 3D cell cultures can also be perfused in our proprietary PerfusionPal system.
Prepare the next generation of biomedical students for the future! Teach them about the advantages of growing cells in three dimensions, as in living tissues. We are happy to demo our 3D cell culture scaffold and organ-on-a-chip-like PerfusionPal system for your undergraduate instructional laboratories. Please reach out if you would like to try our product samples. We look forward to working with you.
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