LC Sciences is a genomics and proteomics company offering innovative and quality products and services. We provide unique and customizable oligonucleotide and peptide microarray products designed for nucleic acid and protein-profiling, biomarker-screening, drug screening, and development of diagnostic-devices
LC Sciences provides a comprehensive Gene Expression Analysis Service featuring the Affymetrix microarray platform.
Affymetrix GeneChips® have shown excellent performance and are proven as a reliable method for gene expression profiling.
Our service is comprehensive and includes target preparation of your total RNA sample, single or dual color labeling, hybridization, image data processing and in-depth data analysis. Two-three weeks after receiving your total RNA samples, we send you original images of the array, raw data and fully analyzed data.
In-depth data analysis of multiple sample group experiments may include:
Microarray Platform: Affymetrix – Genechip® Technology
Starting Sample Material: Total RNA, mRNA, Blood, Cell Pellet, Tissue, FFPE
Sample QC: Agilent 2100 Bioanalyzer RNA, LabChip, Nanodrop ND-1000
Low input total RNA protocol available
Species Covered: All Species for which AffyArrays Exist (inquire about custom arrays)
Advanced Data Analysis Included
Data Delivery Time: 2-3 weeks
Advanced Data Analysis Deliverables:
We use such Affymetrix software systems as MicroArray Suite, MicroDB and Data Mining Tool to provide the data analysis service.
Total RNA-Seq (Whole Transcriptome) sequencing captures a broader range of gene expression changes and enables the detection of novel transcripts in both coding and non-coding RNA species. Ribo-Zero ribosomal RNA reduction chemistry is used in place a poly-A tail selection which efficiently removes ribosomal RNA (rRNA) This process minimizes ribosomal contamination and optimizes the percentage of reads covering RNA species of interest.
LC Sciences provides a one-stop solution (i.e. from sample to data) for Total RNA-Seq using the latest in RNA-Seq technology. The transcriptome profiling and analysis services by LC Sciences are comprehensive and provide the most complete picture of RNA content in your samples. We can help you set up and conduct a high-quality, well-controlled Total RNA-Seq experiment based on the latest deep-sequencing technologies.
Poly(A) RNA seq:
RNA-Seq technology is providing fresh biological insight into the transcriptome. It can be used to determine the structure of genes, their splicing patterns and other post transcriptional modifications, to detect rare and novel transcripts, and to quantify the changing expression levels of each transcript.
LC Sciences provides a one-stop solution (i.e. from sample to data) for RNA-Seq using the latest in rna sequencing technology. The RNA-Seq services provided by LC Sciences are comprehensive and provide the most complete picture of RNA content in your samples. We can help you set up and conduct a high-quality, well-controlled RNA-Seq experiment based on the latest deep-sequencing technologies.
Degradome sequencing (Degradome-Seq), also referred to as parallel analysis of RNA ends (PARE), a modified 5′-rapid amplification of cDNA ends (RACE) with high-throughput next-gen sequencing method. Degradome sequencing provides a comprehensive means of analyzing patterns of RNA degradation.
Degradome sequencing has been use to identify microRNA (miRNA) cleavage sites, because miRNAs can cause endonucleolytic cleavage of mRNA by extensive and often perfect complementarity to mRNAs. Degradome sequencing has revealed many known and novel plant miRNA (siRNA) targets.
LC Sciences provides a one-stop solution for all your microRNA (miRNA) discovery and expression profiling needs. High-throughput sequencing is now available in addition to existing microarray and qRT-PCR profiling services for the most complete picture of microRNA expression in your samples. microRNA sequencing is a new method and a powerful tool to identify and quantitatively decode the entire population of microRNAs in your sample.
This unique combination of the latest deep sequencing technology, advanced bioinformatics, and our innovative µParaflo® custom microarray platform leverages all these technologies to form a comprehensive service package tailored to your specific research needs. Deep sequencing yields results that broadly cover genome-wide miRNAs from samples of various temporal and special origins without the need for any prior sequence knowledge. However, due to the relatively high cost and low throughput nature of sample handling, the systematic follow through of the sequencing discoveries for validation and/or profiling in a reproducible manner could take a long time and be expensive by repetitive sequencing. Microarrays are effective tools to capture the comprehensive sequencing information and to systematically profile and compare the gene expression based on the sequencing findings rapidly, reproducibly, and cost effectively.
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