InVivo Biosystems

Whether you need a screen of an antioxidant for its life promoting effects, or need to determine efficacy in a compound that boosts defective homeostatic mechanisms needed for longevity, we have customized our lifespan/healthspan assay for maximal effect detection. Our proprietary approach has been used by over a dozen labs and pharmas. 

• Translatable Lifespan. Our platform provides lifespan measurements in addition to healthspan data. Often a platform that focuses solely on healthspan will fail to follow an animal for the entirety of their lifespan. Yet one needs lifespan data to enable claims on a compound's ability to extend longevity. Our longevity readout provides both a Kaplan-Meier estimate of survival and a hazard rate curve - two functions that are the industry standard for modeling survival and are widely used in worm, mouse, and human studies.
• Genetic Signature. Our standard platform gives information about how the genetic pathways are regulated in response to the compound, which can provide patentable information about the MoA.
• Export Reports and Access. Our reports deliver graphs that are Marketing & IP ready. We pride ourselves on the thoroughness and interpretability of the results.
• Experts on Tap. All our projects include on-call project status updates, as well as regularly scheduled communication. We also provide on-call consulting and interpretation with our Ph.D. level, subject-matter experts.

Compound Efficacy Assessment and Formulation Analysis. We can quickly and economically test a compound’s ability to extend lifespan and healthspan with large sample sizes (up to 120 million data points) of live animals in less than 5 months and get answers to the following questions:

• Does my compound have a positive or negative effect on lifespan?
• How does my compound affect lifespan AND healthspan?
• Which are the associated signaling pathways that contributed to the life extension?

We are a zebrafish CRO that offers multiple gene-editing services using CRISPR and Tol2 methods. Our services can significantly reduce your experiment design time and can be tailored to different experimental requirements. We offer a variety of service packages including fast, affordable Injection Mix to the comprehensive Full Build service to accommodate your level of expertise and budget.

Injection Mix    Knock-in SDM    Knock-in Tagging     Floxed Gene    Tol2 Transgenesis

• CRISPR Injection Mix: Looking for an easy way to bring CRISPR gene editing into your lab? Choose the level of service that suits you or your lab the best, ranging from standard ready-to-inject mixes to full evaluation of sgRNA cutting and locus editing efficiency. Learn more >>
• Knockout using CRISPR/Cas9: commonly used to understand gene function. We can delete integral domains or the entire the coding sequence of a gene in zebrafish, depending on gene size. We have generated lesions ranging from small indels to full gene deletions. Learn more >>
• Knock-in SDM using CRISPR/Cas9: Create genetic models using zebrafish for understanding gene function, basic biology, and more precisely modeling human diseases. Learn more >>
• Knock-in Tagging using CRISPR/Cas9: Tag your favorite gene at the native locus in your zebrafish line via CRISPR/Cas9 gene editing technology. Choose from a large selection of fluorescent proteins and epitope tags. Learn more >>
• Floxed Gene using CRISPR/Cas9: This can be very useful when studying embryonic lethal genes or for understanding how your gene of interest functions in different tissues. We can create a floxed allele for tissue- or temporal- specific deletion of your gene of interest. Learn more>>
• Tol2 Transgenesis: Insert large content in the zebrafish genome using the Tol2 transposon-based system to analyze tissue-specific gene expression, control the timing of gene expression and humanize the zebrafish. Learn more >>

Genome editing types include Knockout, Knock-in, MosSCI and Extrachromosomal Array. We offer a variety of services packages to fit the needs and budget of your lab from the fast, affordable Custom Injection Services to the comprehensive Full Build service packages, allowing you to customize the components you need to accomplish your gene editing goals.

• Design, construct, and deliver custom reagents or genetically edited C. elegans optimized to your specifications.
• Collaborate on project design to ensure transparency in the genome editing process and guarantee that you receive the transgenic best suited for your experiments. 

Visit InVivo Biosystem's C elegans Transgenic Services to learn more about the pricing and process. 

We can test drug toxicity and dosage in C. elegans using a combination of assays to balance between catching all the compounds that pose a hazard and excluding those that do not pose a risk. Learn more. 

Conduct pathogenicity assessment of variants in disease related genes.

• Feasibility study of gene(s) for animal modeling. Create and phenotype loss of function knockout mutant lines. Rescue loss of function with expression o human proteins.
• Model known benign and known pathogenic variants. Create and phenotype 5 benign variants and 6 pathogenic variants. Determine the predictive ability of the assay based on the known variants.
• Recommendation of classification of variants based on phenotype(s). Create and phenotype variants of unknown significance and predict pathogenicity.

We can perform expression and knock-out studies to identify and validate drug targets

and molecular mechanism of action (MMOA).

Our standard package includes:

• Genetic epistasis studies: Discovery and confirmation of associated genes using overexpression, RNAi knockdown, antisense oligos, and full deletions.
• Gene expression profiling: RNAseq transcriptome analysis, western blotting, fluorescent/ luciferase tagging.
• Full service data pipelines and phenotypic analysis.

We have found whole gene humanization can create a platform for highly translatable results in a model organism. We can conduct pathogenicity assessment of variants in disease related genes. Our standard process includes:

• Feasibility study of gene(s) for animal modeling. Create and phenotype loss of function knockout mutant lines. Rescue loss of function with expression o human proteins.
• Model known benign and known pathogenic variants. Create and phenotype 5 benign variants and 6 pathogenic variants. Determine the predictive ability of the assay based on the known variants.
• Recommendation of classification of variants based on phenotype(s). Create and phenotype variants of unknown significance and predict pathogenicity.

Our platform using whole gene humanization can create highly translatable results in a model organism.