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Kitos Biotech srls

Sassari, IT

We support research centers, biotechs and pharmas in the field of medicinal chemistry and oncology to develop their products at preclinical stage with our scientific expertise and technology. We help in the development of a scientifically sound and cost-effective experimental plan to evaluate in vitro drug efficacy and mechanism of action of experimental products, taking advantage of automation systems, live-cell imaging and more than 100 in-house cell lines. We perform experiments and analyze data in our laboratory, providing ready-to-use results for customer's pipelines.

Recent Publications
- Marchesi I, Sanna L, Fais M, Fiorentino FP, Giordano A, Bagella L. 12-O-Tetradecanoylphorbol-13-acetate and EZH2 inhibition: a novel approach for promoting myogenic differentiation in embryonal rhabdomyosarcoma cells. Journal of Cellular Physiology 233(3):2360-2365, 2018.
- Fiorentino FP, Bagella L, Marchesi I. A... Show more »

We support research centers, biotechs and pharmas in the field of medicinal chemistry and oncology to develop their products at preclinical stage with our scientific expertise and technology. We help in the development of a scientifically sound and cost-effective experimental plan to evaluate in vitro drug efficacy and mechanism of action of experimental products, taking advantage of automation systems, live-cell imaging and more than 100 in-house cell lines. We perform experiments and analyze data in our laboratory, providing ready-to-use results for customer's pipelines.

Recent Publications
- Marchesi I, Sanna L, Fais M, Fiorentino FP, Giordano A, Bagella L. 12-O-Tetradecanoylphorbol-13-acetate and EZH2 inhibition: a novel approach for promoting myogenic differentiation in embryonal rhabdomyosarcoma cells. Journal of Cellular Physiology 233(3):2360-2365, 2018.
- Fiorentino FP, Bagella L, Marchesi I. A New Parameter of Growth Inhibition for Cell Proliferation Assays. Journal of Cellular Physiology, 233(5):4106-41152018, 2018.
- Nieddu V, Pinna G, Marchesi I, Sanna L, Asproni B, Pinna GA, Bagella LM, Murineddu G. Synthesis and antineoplastic evaluation of novel unsymmetrical 1,3,4-oxadiazoles. Journal of Medicinal Chemistry, 59 (23), pp 10451–10469, 2016.
- Kato F, Fiorentino FP, Alibés A, Perucho M, Sánchez-Céspedes M, Kohno T, Yokota J. MYCL is a Target of a BET Bromodomain Inhibitor, JQ1, on Growth Suppression Efficacy in Small Cell Lung Cancer Cells. Oncotarget 22;7(47):77378-77388, 2016.

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Cell Proliferation Assays
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Western Blot
Price on request

To determine protein levels of your target of interest. We perform protein extraction and western blot with commercial antibodies to compare protein levels in distinct cell lines or to evaluate changes in protein expression following treatment with your compound of interest. Cell lines can be chosen from our in-house panel or... Show more »

To determine protein levels of your target of interest. We perform protein extraction and western blot with commercial antibodies to compare protein levels in distinct cell lines or to evaluate changes in protein expression following treatment with your compound of interest. Cell lines can be chosen from our in-house panel or shipped from you to our laboratory.

Features of the assay

  • We support you designing the experimental plan. Based on more than 10 years of experience and more than 500 western blot performed, we can help you deciding which antibody to order and test, which cell lines to include as control, and which protocol best fits your target.
  • We perform the entire experimental plan and analysis in our laboratory, from compound treatment of cells to image acquisition.
  • We support you with data interpretation.
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Cell Viability & Proliferation Assays
Price on request

We offer a robust assay to determine kinetic of compound potency and efficacy based on live-cell imaging.

Features of the assay

  • Number of live/dead cells can be measured multiple times over a short (time-lapse) or a long period of treatment.
  • IC50, relative growth and percentage of growth inhibition (IC50, GI50, TGI... Show more »

We offer a robust assay to determine kinetic of compound potency and efficacy based on live-cell imaging.

Features of the assay

  • Number of live/dead cells can be measured multiple times over a short (time-lapse) or a long period of treatment.
  • IC50, relative growth and percentage of growth inhibition (IC50, GI50, TGI and LC50) calculations.
  • Available in high-throughput (HTS).
  • Accurate
    • Number of cells is estimated by counting nuclei.
    • Fraction of dead cells is excluded by live cell counting.
    • Multiple reads of the same plate allow well-to-well normalized data analysis.
  • Informative on the mechanism of action
    • It discriminates between cytostatic and cytotoxic compounds.
    • Phase contrast images can be available on request to evaluate cell morphology.
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Live-cell
Cancer
Oncology
in vitro toxicity
Cytotoxicity
Cell-based assays
BioTek Cytation 5
Gilson Pipetmax
SiR-Hoechst
Promega CellTox
BioTek Gen5
Kinetic
live-cell imaging
Cytotoxicity Assays
Price on request

We offer a robust assay to determine kinetic of compound potency and efficacy based on live-cell imaging.

Features of the assay

  • Number of live/dead cells can be measured multiple times over a short (time-lapse) or a long period of treatment.
  • IC50, relative growth and percentage of growth inhibition (IC50, GI50, TGI... Show more »

We offer a robust assay to determine kinetic of compound potency and efficacy based on live-cell imaging.

Features of the assay

  • Number of live/dead cells can be measured multiple times over a short (time-lapse) or a long period of treatment.
  • IC50, relative growth and percentage of growth inhibition (IC50, GI50, TGI and LC50) calculations.
  • Available in high-throughput (HTS).
  • Accurate
    • Number of cells is estimated by counting nuclei.
    • Fraction of dead cells is excluded by live cell counting.
    • Multiple reads of the same plate allow well-to-well normalized data analysis.
  • Informative on the mechanism of action
    • It discriminates between cytostatic and cytotoxic compounds.
    • Phase contrast images can be available on request to evaluate cell morphology.
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Cancer
Oncology
in vitro toxicity
Cell Line
Cytotoxicity
Cell-based assays
BioTek Cytation 5
Gilson Pipetmax
SiR-Hoechst
Promega CellTox
BioTek Gen5
Kinetic
live-cell imaging
3D Cell Culture Models
Price on request

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
    -... Show more »

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
  • Accurate
    • A single spheroid forms in each well of the plate. Spheroid sizes are estimated by measuring their area and calculating the volume.
    • Multiple reads at various time points are carried out to obtain spheroid area values normalized on initial spheroid sizes.
  • Informative on the mechanism of action
    • It discriminates between cytostatic and cytotoxic compounds.
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Cell Cycle Analysis
Price on request

An alternative method to flow cytometry to assess cell cycle using fluorescence microscopy and cell imaging.

Features of the assay

  • Available for high-content analysis.
  • Quan­titative, fully automated and unbiased cell cycle distributions.
  • It can be multiplexed with other of our cell-based assays.

An alternative method to flow cytometry to assess cell cycle using fluorescence microscopy and cell imaging.

Features of the assay

  • Available for high-content analysis.
  • Quan­titative, fully automated and unbiased cell cycle distributions.
  • It can be multiplexed with other of our cell-based assays.
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Cancer
Oncology
Fluorescent labeling
Cell Line
BioTek Cytation 5
Gilson Pipetmax
cell imaging
3D Tumor Model
Price on request

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
    -... Show more »

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
  • Accurate
    • A single spheroid forms in each well of the plate. Spheroid sizes are estimated by measuring their area and calculating the volume.
    • Multiple reads at various time points are carried out to obtain spheroid area values normalized on initial spheroid sizes.
  • Informative on the mechanism of action
    • It discriminates between cytostatic and cytotoxic compounds.
« Show less
Cancer
Oncology
in vitro toxicity
Cytotoxicity
Cell-based assays
BioTek Cytation 5
Gilson Pipetmax
Promega CellTox
live-cell imaging
3D culture
3D Cell-Based Assays
Price on request

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
    -... Show more »

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
  • Accurate
    • A single spheroid forms in each well of the plate. Spheroid sizes are estimated by measuring their area and calculating the volume.
    • Multiple reads at various time points are carried out to obtain spheroid area values normalized on initial spheroid sizes.
  • Informative on the mechanism of action
    • It discriminates between cytostatic and cytotoxic compounds.
« Show less
Cancer
Oncology
in vitro toxicity
Cytotoxicity
Cell-based assays
BioTek Cytation 5
Gilson Pipetmax
Promega CellTox
live-cell imaging
3D culture
Cell Viability Assays
Price on request

We offer a reliable and broadly employed cell viability assay to determine compound efficacy.

Features of the assay

  • IC50 calculation.
  • Number of viable cells in culture is estimated with Promega Cell-Titer Glo® assay.

We offer a reliable and broadly employed cell viability assay to determine compound efficacy.

Features of the assay

  • IC50 calculation.
  • Number of viable cells in culture is estimated with Promega Cell-Titer Glo® assay.
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Promega's Cell Titer Glo reagent
Cancer
Oncology
in vitro toxicity
Cytotoxicity
Cell-based assays
Gilson Pipetmax
Promega Glomax Discovery
Apoptosis Assays
Price on request

We offer a cell-based assay to determine compound-mediated induction of apoptosis by measuring relative enzymatic activities of caspase 3/7, 8 and 9 in cell culture.

Features of the assay

  • Caspases activities are independently measured with Promega Caspase-Glo® 3/7, 8 or 9 assays.
  • Induction of apoptosis can be... Show more »

We offer a cell-based assay to determine compound-mediated induction of apoptosis by measuring relative enzymatic activities of caspase 3/7, 8 and 9 in cell culture.

Features of the assay

  • Caspases activities are independently measured with Promega Caspase-Glo® 3/7, 8 or 9 assays.
  • Induction of apoptosis can be correlated with cell viability by multiplexing the assay with our kinetic of cytotoxicity and proliferation assay.
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Cancer
Oncology
Antioxidant Assays
Price on request

We offer a cell-based assay to measure anti-oxidant activity of tested compound by measuring relative levels of hydrogen peroxide, a reactive oxigen species, in cell culture.

Features of the assay

  • Levels of H2O2 are measured with Promega ROS-Glo® H2O2 assay.
  • Antioxidant activity can be correlated with cell viability... Show more »

We offer a cell-based assay to measure anti-oxidant activity of tested compound by measuring relative levels of hydrogen peroxide, a reactive oxigen species, in cell culture.

Features of the assay

  • Levels of H2O2 are measured with Promega ROS-Glo® H2O2 assay.
  • Antioxidant activity can be correlated with cell viability by multiplexing the assay with our kinetic of cytotoxicity and proliferation assay.
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Histone Deacetylase (HDAC) Assays
Price on request

We offer a cell-based assay to determine potency of histone deacetylase (HDAC) class I and II inhibitors by measuring relative enzymatic activity of HDAC I/II in cell culture.

Features of the assay

  • Activities of HDAC I/II are measured with Promega HDAC-Glo® assay.
  • HDAC inhibitor potency can be correlated with cell... Show more »

We offer a cell-based assay to determine potency of histone deacetylase (HDAC) class I and II inhibitors by measuring relative enzymatic activity of HDAC I/II in cell culture.

Features of the assay

  • Activities of HDAC I/II are measured with Promega HDAC-Glo® assay.
  • HDAC inhibitor potency can be correlated with cell viability by multiplexing the assay with our kinetic of cytotoxicity and proliferation assay.
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Multidrug Resistance (MDR) Testing
Price on request

We offer a citotoxicity and proliferation assay to determine contribution of ABC transporters to cancer cell chemoresistance of tested compound based on live-cell imaging.

Features of the assay

  • Cells are treated with three selective inhibitors of ABC transporters together with tested compound.
  • Number of live/dead... Show more »

We offer a citotoxicity and proliferation assay to determine contribution of ABC transporters to cancer cell chemoresistance of tested compound based on live-cell imaging.

Features of the assay

  • Cells are treated with three selective inhibitors of ABC transporters together with tested compound.
  • Number of live/dead cells is measured with our kinetic of cytotoxicity and proliferation assay.
  • Relative cell number, relative growth and percentage of growth inhibition calculations.
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Cell-Based Compound Screening
Price on request

We offer a cell-based screening to test compound libraries of your choice in your model of interest.

Features of the assay

  • We have available various commercial libraries of known compounds from Selleckchem:

    • PI3K/Akt Inhibitor Library - 118 inhibitors
    • Epigenetics Compound Library - 182 small molecules
      -... Show more »

We offer a cell-based screening to test compound libraries of your choice in your model of interest.

Features of the assay

  • We have available various commercial libraries of known compounds from Selleckchem:

    • PI3K/Akt Inhibitor Library - 118 inhibitors
    • Epigenetics Compound Library - 182 small molecules
    • Anti-cancer Compound Library - 422 compounds
    • Apoptosis Compound Library - 101 small molecules
    • FDA-approved Drug Library - 1443 drugs
    • Tyrosine Kinase Inhibitor Library - 171 inhibitors
    • Autophagy Compound Library - 154 inhibitors
    • MAPK Inhibitor Librabry - 61 inhibitors
    • Natural Product Library - 133 products
    • Stem Cell Signaling Compound Library - 89 inhibitors
    • Protease Inhibitor Library - 53 inhibitors
  • We can test these libraries in our cell lines or in cells provided by customers.

  • We can employ any of our assays available in high-throughput:

    • Kinetic of Cytotoxicity and Proliferation assay
    • 3D Spheroid Drug Sensitivity assay
    • Cell Viability assay
    • Antioxidant assay
    • Histone Deacetylase Activity assay
  • We can evaluate the combinatorial efficacy of your favorite compound with these libraries to perform drug combination studies.

  • Selected hits will be validated with the corresponding assay with 11 concentrations of compound.

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Screening
Cell-based assays
live-cell imaging
Cell Migration Assays
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Clinical Research
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Clinical Laboratory Services
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Clinical Microbiology, Virology and Parasitology
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Biology
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Cell-Based Assays
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Cell Invasion & Migration Assays
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Cell Death Assays
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Specialized Cell-Based Assays
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Cells and Tissues
Price on request
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Mammalian Cell Culture
Price on request
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Cytometry
Price on request
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In vitro Disease Models
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Biochemistry & Molecular Biology
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Biochemical Assays
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Enzyme Assays
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Hydrolase Assays
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Pharmacology & Toxicology
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Toxicology
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Drug Discovery & Development
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Drug Discovery
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Hit Identification
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Protein Services
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Protein Services

Protein Services

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Protein Expression Visualization
Price on request

Protein Expression Visualization Services

Protein Expression Visualization Services

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Custom Cell-Based Assays
Price on request
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Kitos Biotech srls has not received any ratings.

Missing Avatar
2018-07-30 04:59:49 -0700

We worked with Kitos for a project last year and we were very happy with our collaboration. Kitos is led by motivated scientists and professionals that guarantee the accuracy of their results and are willing to troubleshoot with you whatever aspects you might deem necessary for the success of your project.

Endorse this lab