Kitos Biotech

- Send your compounds as lyophilized or as stock solution.

- Evaluate gene expression levels of ∼2,600 genes involved in 24 cancer-related molecular pathways and/or ∼2,100 miRNA transcripts in our panel of cell lines treated with your favorite compounds.

- Cancer-related gene or miRNA expression levels are determined in our cell lines grown as monolayer or 3D tumor spheroids.

- Bioinformatic analysis is included.

- We manage all the process from cell culture to data analysis.

- You send us the compounds and directly receive ready-to-use results.

- Flexible project planning based on your needs.

- Assess autophagy induction by your compounds in our panel of authenticated cell lines.

- Send your compounds lyophilized or as stock solutions.

- Autophagy is assessed by fluorescence microscopy.

- Mid-throughput screening is performed thanks to lab automation.

- Receive a detailed study report in approximatively one or two months.

- Flexible project planning based on needs.

- IC50, GI50, TGI, LC50 calculations.

- Compounds are accepted both as lyophilized or as stock solution.

- Mid-throughput screening is performed thanks to lab automation.

- Receive a detailed study report in approximatively one or two months.

- Price is referred to technical triplicate for each sample (1 concentration of 1 compound assessed in 1 cell line).

- A multiplexed viability and cytotoxicity assay is performed to distinguish cytostatic and cytotoxic effects.

- Flexible project planning based on needs.

- IC50, GI50, TGI, LC50 calculations.

- Compounds are accepted both as lyophilized or as stock solution.

- Mid-throughput screening is performed thanks to lab automation.

- Receive a detailed study report in approximatively one or two months.

- Price is referred to technical triplicate for each sample (1 concentration of 1 compound assessed in 1 cell line).

- A multiplexed viability and cytotoxicity assay is performed to distinguish cytostatic and cytotoxic effects.

- Flexible project planning based on your needs.

- Assess autophagy induction by your compounds in our panel of authenticated cell lines.

- Send your compounds lyophilized or as stock solutions.

- Autophagy is assessed by fluorescence microscopy.

- Mid-throughput screening is performed thanks to lab automation.

- Receive a detailed study report in approximatively one or two months.

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

• Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
• Available in high-throughput (HTS).
• Accurate
  • A single spheroid forms in each well of the plate. Spheroid sizes are estimated by measuring their area and calculating the volume.
  • Multiple reads at various time points are carried out to obtain spheroid area values normalized on initial spheroid sizes.
• Informative on the mechanism of action
  • It discriminates between cytostatic and cytotoxic compounds.

An alternative method to flow cytometry to assess cell cycle using fluorescence microscopy and cell imaging.

Features of the assay

• Available for high-content analysis.
• Quan­titative, fully automated and unbiased cell cycle distributions.
• It can be multiplexed with other of our cell-based assays.

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

• Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
• Available in high-throughput (HTS).
• Accurate
  • A single spheroid forms in each well of the plate. Spheroid sizes are estimated by measuring their area and calculating the volume.
  • Multiple reads at various time points are carried out to obtain spheroid area values normalized on initial spheroid sizes.
• Informative on the mechanism of action
  • It discriminates between cytostatic and cytotoxic compounds.

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

• Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
• Available in high-throughput (HTS).
• Accurate
  • A single spheroid forms in each well of the plate. Spheroid sizes are estimated by measuring their area and calculating the volume.
  • Multiple reads at various time points are carried out to obtain spheroid area values normalized on initial spheroid sizes.
• Informative on the mechanism of action
  • It discriminates between cytostatic and cytotoxic compounds.

We offer a reliable and broadly employed cell viability assay to determine compound efficacy.

Features of the assay

• IC50 calculation.
• Number of viable cells in culture is estimated with Promega Cell-Titer Glo® assay.

- Evaluate induction of apoptosis by your compounds on our panel of cell lines.

- The assay measures ex vivo enzymatic activities of caspases 3/7, 8 and 9.

We offer a cell-based assay to measure anti-oxidant activity of tested compound by measuring relative levels of hydrogen peroxide, a reactive oxigen species, in cell culture.

Features of the assay

• Levels of H2O2 are measured with Promega ROS-Glo® H2O2 assay.
• Antioxidant activity can be correlated with cell viability by multiplexing the assay with our kinetic of cytotoxicity and proliferation assay.

We offer a cell-based assay to determine potency of histone deacetylase (HDAC) class I and II inhibitors by measuring relative enzymatic activity of HDAC I/II in cell culture.

Features of the assay

• Activities of HDAC I/II are measured with Promega HDAC-Glo® assay.
• HDAC inhibitor potency can be correlated with cell viability by multiplexing the assay with our kinetic of cytotoxicity and proliferation assay.

We offer a citotoxicity and proliferation assay to determine contribution of ABC transporters to cancer cell chemoresistance of tested compound based on live-cell imaging.

Features of the assay

• Cells are treated with three selective inhibitors of ABC transporters together with tested compound.
• Number of live/dead cells is measured with our kinetic of cytotoxicity and proliferation assay.
• Relative cell number, relative growth and percentage of growth inhibition calculations.

We offer a cell-based screening to test compound libraries of your choice in your model of interest.

Features of the assay

• We have available various commercial libraries of known compounds from Selleckchem:

  • PI3K/Akt Inhibitor Library - 118 inhibitors
  • Epigenetics Compound Library - 182 small molecules
  • Anti-cancer Compound Library - 422 compounds
  • Apoptosis Compound Library - 101 small molecules
  • FDA-approved Drug Library - 1443 drugs
  • Tyrosine Kinase Inhibitor Library - 171 inhibitors
  • Autophagy Compound Library - 154 inhibitors
  • MAPK Inhibitor Librabry - 61 inhibitors
  • Natural Product Library - 133 products
  • Stem Cell Signaling Compound Library - 89 inhibitors
  • Protease Inhibitor Library - 53 inhibitors

• We can test these libraries in our cell lines or in cells provided by customers.

• We can employ any of our assays available in high-throughput:

  • Kinetic of Cytotoxicity and Proliferation assay
  • 3D Spheroid Drug Sensitivity assay
  • Cell Viability assay
  • Antioxidant assay
  • Histone Deacetylase Activity assay

• We can evaluate the combinatorial efficacy of your favorite compound with these libraries to perform drug combination studies.

• Selected hits will be validated with the corresponding assay with 11 concentrations of compound.

Protein Expression Visualization Services

Protein Services

Cellular Senescence Assay

- Flexible project planning based on your needs.

- Assess induction of cellular senescence by your compounds in our panel of authenticated cell lines.

- Send your compounds lyophilized or as stock solutions.

- SA-Beta-Gal is assessed by fluorescence microscopy.

- Mid-throughput screening is performed thanks to lab automation.

- Receive a detailed study report in approximatively one or two months