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Kitos Biotech srls

Sassari, Sardegna, IT

Kitos Biotech is an italian private company founded in 2016 by two cell and molecular biologists, Francesco P. Fiorentino and Irene Marchesi. The laboratory is located in the scientific park Porto Conte Ricerche, near Alghero (Sardegna, Italia).
Kitos biotech offers high quality cell-based assay services taking advantage of cutting-edge automation systems.

  • No intermediaries: From the first time you contact us, you’ll speak with a PhD level experienced researcher that will help you to obtain the perfect assay you wish.​

  • Unique assays: Each project is unique, as well as the experimental conditions of each assays. We are totally flexible in adjusting all the variables of our assays to obtain the right conditions for your requests.

  • Scientific reliability: We know the value of reproducibility. We will never perform an assay using cells in bad culture conditions or without proper controls. We provide... Show more »

Kitos Biotech is an italian private company founded in 2016 by two cell and molecular biologists, Francesco P. Fiorentino and Irene Marchesi. The laboratory is located in the scientific park Porto Conte Ricerche, near Alghero (Sardegna, Italia).
Kitos biotech offers high quality cell-based assay services taking advantage of cutting-edge automation systems.

  • No intermediaries: From the first time you contact us, you’ll speak with a PhD level experienced researcher that will help you to obtain the perfect assay you wish.​

  • Unique assays: Each project is unique, as well as the experimental conditions of each assays. We are totally flexible in adjusting all the variables of our assays to obtain the right conditions for your requests.

  • Scientific reliability: We know the value of reproducibility. We will never perform an assay using cells in bad culture conditions or without proper controls. We provide exclusively services with high technical quality.

  • No wasted time: We know that time matters. We are focused to provide results in a short time, taking advantage of automation systems and our experience. ​We have just one exception in being fast: quality comes first.

  • The mind of a researcher: We are researchers. We think as researchers. We do not only provide high quality custom assays, but we analyze data and provide ready-to-publish results putting effort as we do for our papers.

  • More than 100 in-house cell lines.

  • Cell cultures in both monolayer and 3D.

Recent Publications
- Fiorentino FP, Bagella L, Marchesi I. A New Parameter of Growth Inhibition for Cell Proliferation Assays. Journal of Cellular Physiology 2017 Oct 12. [Epub ahead of print].
- Marchesi I, Sanna L, Fais M, Fiorentino FP, Giordano A, Bagella L. 12-O-Tetradecanoylphorbol-13-acetate and EZH2 inhibition: a novel approach for promoting myogenic differentiation in embryonal rhabdomyosarcoma cells. Journal of Cellular Physiology 2017 Jul 19. [Epub ahead of print].
- Tokgun O, Fiorentino FP, Tokgun PE, Yokota J, Akca H. Design of a Lentiviral Vector for the Inducible Expression of MYC: A New Strategy for Construction Approach. Molecular Biotechnology Jun;59(6):200-206, 2017.
- Sanna L, Ortu S, Marchesi I, Bagella P, Fais M, Fiore V, Vidili G, Bagella L, Babudieri S. Evaluation of EZH2 expression in hepatitis B patients. Infectiuos Diseases and Tropical Medicine, 2 (4): e349, 2016.
- Nieddu V, Pinna G, Marchesi I, Sanna L, Asproni B, Pinna GA, Bagella LM, Murineddu G. Synthesis and antineoplastic evaluation of novel unsymmetrical 1,3,4-oxadiazoles. Journal of Medicinal Chemistry, 59 (23), pp 10451–10469, 2016.
- Kato F, Fiorentino FP, Alibés A, Perucho M, Sánchez-Céspedes M, Kohno T, Yokota J. MYCL is a Target of a BET Bromodomain Inhibitor, JQ1, on Growth Suppression Efficacy in Small Cell Lung Cancer Cells. Oncotarget 2016, 22;7(47):77378-77388.
- Fiorentino FP, Tokgün E, Solé-Sánchez S, Giampaolo S, Tokgün O, Jauset T, Kohno T, Perucho M, Soucek L, Yokota J. Growth suppression by MYC inhibition in small cell lung cancer cells with TP53 and RB1 inactivation. Oncotarget 2016, 7(21):31014-28.
- Bagella L, Marchesi I. SFK Inhibitors as a New Strategy for RMS Treatment. Chemotherapy: Open Access. 5 (2): 1000189, 2016.
- Marchesi I, Bagella L. Editorial: Targeting Enhancer of Zeste Homolog 2 as a promising strategy for cancer treatment. World Journal of Clinical Oncology, 7(2):135-48, 2016.

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Kitos Biotech srls has not listed any services.

Cell Viability & Proliferation Assays
Price on request

We offer a robust assay to determine kinetic of compound potency and efficacy based on live-cell imaging.

Features of the assay

  • Number of live/dead cells can be measured multiple times over a short (time-lapse) or a long period of treatment.
  • IC50, relative growth and percentage of growth inhibition (IC50, GI50, TGI... Show more »

We offer a robust assay to determine kinetic of compound potency and efficacy based on live-cell imaging.

Features of the assay

  • Number of live/dead cells can be measured multiple times over a short (time-lapse) or a long period of treatment.
  • IC50, relative growth and percentage of growth inhibition (IC50, GI50, TGI and LC50) calculations.
  • Available in high-throughput (HTS).
  • Accurate
    • Number of cells is estimated by counting nuclei.
    • Fraction of dead cells is excluded by live cell counting.
    • Multiple reads of the same plate allow well-to-well normalized data analysis.
  • Informative on the mechanism of action
    • It discriminates between cytostatic and cytotoxic compounds.
    • Phase contrast images can be available on request to evaluate cell morphology.
« Show less
Live-cell
Cancer
Oncology
in vitro toxicity
Cytotoxicity
Cell-based assays
BioTek Cytation 5
Gilson Pipetmax
SiR-Hoechst
Promega CellTox
BioTek Gen5
Kinetic
live-cell imaging
Cytotoxicity Assays
Price on request

We offer a robust assay to determine kinetic of compound potency and efficacy based on live-cell imaging.

Features of the assay

  • Number of live/dead cells can be measured multiple times over a short (time-lapse) or a long period of treatment.
  • IC50, relative growth and percentage of growth inhibition (IC50, GI50, TGI... Show more »

We offer a robust assay to determine kinetic of compound potency and efficacy based on live-cell imaging.

Features of the assay

  • Number of live/dead cells can be measured multiple times over a short (time-lapse) or a long period of treatment.
  • IC50, relative growth and percentage of growth inhibition (IC50, GI50, TGI and LC50) calculations.
  • Available in high-throughput (HTS).
  • Accurate
    • Number of cells is estimated by counting nuclei.
    • Fraction of dead cells is excluded by live cell counting.
    • Multiple reads of the same plate allow well-to-well normalized data analysis.
  • Informative on the mechanism of action
    • It discriminates between cytostatic and cytotoxic compounds.
    • Phase contrast images can be available on request to evaluate cell morphology.
« Show less
Cancer
Oncology
in vitro toxicity
Cell Line
Cytotoxicity
Cell-based assays
BioTek Cytation 5
Gilson Pipetmax
SiR-Hoechst
Promega CellTox
BioTek Gen5
Kinetic
live-cell imaging
3D Cell Culture Models
Price on request

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
    -... Show more »

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
  • Accurate
    • A single spheroid forms in each well of the plate. Spheroid sizes are estimated by measuring their area and calculating the volume.
    • Multiple reads at various time points are carried out to obtain spheroid area values normalized on initial spheroid sizes.
  • Informative on the mechanism of action
    • It discriminates between cytostatic and cytotoxic compounds.
« Show less
Cell Cycle Analysis
Price on request

An alternative method to flow cytometry to assess cell cycle using fluorescence microscopy and cell imaging.

Features of the assay

  • Available for high-content analysis.
  • Quan­titative, fully automated and unbiased cell cycle distributions.
  • It can be multiplexed with other of our cell-based assays.

An alternative method to flow cytometry to assess cell cycle using fluorescence microscopy and cell imaging.

Features of the assay

  • Available for high-content analysis.
  • Quan­titative, fully automated and unbiased cell cycle distributions.
  • It can be multiplexed with other of our cell-based assays.
« Show less
Cancer
Oncology
Fluorescent labeling
Cell Line
BioTek Cytation 5
Gilson Pipetmax
cell imaging
3D Tumor Model
Price on request

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
    -... Show more »

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
  • Accurate
    • A single spheroid forms in each well of the plate. Spheroid sizes are estimated by measuring their area and calculating the volume.
    • Multiple reads at various time points are carried out to obtain spheroid area values normalized on initial spheroid sizes.
  • Informative on the mechanism of action
    • It discriminates between cytostatic and cytotoxic compounds.
« Show less
Cancer
Oncology
in vitro toxicity
Cytotoxicity
Cell-based assays
BioTek Cytation 5
Gilson Pipetmax
Promega CellTox
live-cell imaging
3D culture
3D Cell-Based Assays
Price on request

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
    -... Show more »

We offer a citotoxicity and proliferation assay to determine kinetic of compound efficacy on 3D tumor spheroids based on live-cell imaging.

Features of the assay

  • Spheroid size and cell death can be measured multiple times over a short (time-lapse) or a long period of time.
  • Available in high-throughput (HTS).
  • Accurate
    • A single spheroid forms in each well of the plate. Spheroid sizes are estimated by measuring their area and calculating the volume.
    • Multiple reads at various time points are carried out to obtain spheroid area values normalized on initial spheroid sizes.
  • Informative on the mechanism of action
    • It discriminates between cytostatic and cytotoxic compounds.
« Show less
Cancer
Oncology
in vitro toxicity
Cytotoxicity
Cell-based assays
BioTek Cytation 5
Gilson Pipetmax
Promega CellTox
live-cell imaging
3D culture
Cell Viability Assays
Price on request

We offer a reliable and broadly employed cell viability assay to determine compound efficacy.

Features of the assay

  • IC50 calculation.
  • Number of viable cells in culture is estimated with Promega Cell-Titer Glo® assay.

We offer a reliable and broadly employed cell viability assay to determine compound efficacy.

Features of the assay

  • IC50 calculation.
  • Number of viable cells in culture is estimated with Promega Cell-Titer Glo® assay.
« Show less
Promega's Cell Titer Glo reagent
Cancer
Oncology
in vitro toxicity
Cytotoxicity
Cell-based assays
Gilson Pipetmax
Promega Glomax Discovery
Apoptosis Assays
Price on request

We offer a cell-based assay to determine compound-mediated induction of apoptosis by measuring relative enzymatic activities of caspase 3/7, 8 and 9 in cell culture.

Features of the assay

  • Caspases activities are independently measured with Promega Caspase-Glo® 3/7, 8 or 9 assays.
  • Induction of apoptosis can be... Show more »

We offer a cell-based assay to determine compound-mediated induction of apoptosis by measuring relative enzymatic activities of caspase 3/7, 8 and 9 in cell culture.

Features of the assay

  • Caspases activities are independently measured with Promega Caspase-Glo® 3/7, 8 or 9 assays.
  • Induction of apoptosis can be correlated with cell viability by multiplexing the assay with our kinetic of cytotoxicity and proliferation assay.
« Show less
Cancer
Oncology
Antioxidant Assays
Price on request

We offer a cell-based assay to measure anti-oxidant activity of tested compound by measuring relative levels of hydrogen peroxide, a reactive oxigen species, in cell culture.

Features of the assay

  • Levels of H2O2 are measured with Promega ROS-Glo® H2O2 assay.
  • Antioxidant activity can be correlated with cell viability... Show more »

We offer a cell-based assay to measure anti-oxidant activity of tested compound by measuring relative levels of hydrogen peroxide, a reactive oxigen species, in cell culture.

Features of the assay

  • Levels of H2O2 are measured with Promega ROS-Glo® H2O2 assay.
  • Antioxidant activity can be correlated with cell viability by multiplexing the assay with our kinetic of cytotoxicity and proliferation assay.
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Histone Deacetylase (HDAC) Assays
Price on request

We offer a cell-based assay to determine potency of histone deacetylase (HDAC) class I and II inhibitors by measuring relative enzymatic activity of HDAC I/II in cell culture.

Features of the assay

  • Activities of HDAC I/II are measured with Promega HDAC-Glo® assay.
  • HDAC inhibitor potency can be correlated with cell... Show more »

We offer a cell-based assay to determine potency of histone deacetylase (HDAC) class I and II inhibitors by measuring relative enzymatic activity of HDAC I/II in cell culture.

Features of the assay

  • Activities of HDAC I/II are measured with Promega HDAC-Glo® assay.
  • HDAC inhibitor potency can be correlated with cell viability by multiplexing the assay with our kinetic of cytotoxicity and proliferation assay.
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Multidrug Resistance Testing
Price on request

We offer a citotoxicity and proliferation assay to determine contribution of ABC transporters to cancer cell chemoresistance of tested compound based on live-cell imaging.

Features of the assay

  • Cells are treated with three selective inhibitors of ABC transporters together with tested compound.
  • Number of live/dead... Show more »

We offer a citotoxicity and proliferation assay to determine contribution of ABC transporters to cancer cell chemoresistance of tested compound based on live-cell imaging.

Features of the assay

  • Cells are treated with three selective inhibitors of ABC transporters together with tested compound.
  • Number of live/dead cells is measured with our kinetic of cytotoxicity and proliferation assay.
  • Relative cell number, relative growth and percentage of growth inhibition calculations.
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Cell-Based Compound Screening
Price on request

We offer a cell-based screening to test compound libraries of your choice in your model of interest.

Features of the assay

  • We have available various commercial libraries of known compounds from Selleckchem:

    • PI3K/Akt Inhibitor Library - 118 inhibitors
    • Epigenetics Compound Library - 182 small molecules
      -... Show more »

We offer a cell-based screening to test compound libraries of your choice in your model of interest.

Features of the assay

  • We have available various commercial libraries of known compounds from Selleckchem:

    • PI3K/Akt Inhibitor Library - 118 inhibitors
    • Epigenetics Compound Library - 182 small molecules
    • Anti-cancer Compound Library - 422 compounds
    • Apoptosis Compound Library - 101 small molecules
    • FDA-approved Drug Library - 1443 drugs
    • Tyrosine Kinase Inhibitor Library - 171 inhibitors
    • Autophagy Compound Library - 154 inhibitors
    • MAPK Inhibitor Librabry - 61 inhibitors
    • Natural Product Library - 133 products
    • Stem Cell Signaling Compound Library - 89 inhibitors
    • Protease Inhibitor Library - 53 inhibitors
  • We can test these libraries in our cell lines or in cells provided by customers.

  • We can employ any of our assays available in high-throughput:

    • Kinetic of Cytotoxicity and Proliferation assay
    • 3D Spheroid Drug Sensitivity assay
    • Cell Viability assay
    • Antioxidant assay
    • Histone Deacetylase Activity assay
  • We can evaluate the combinatorial efficacy of your favorite compound with these libraries to perform drug combination studies.

  • Selected hits will be validated with the corresponding assay with 11 concentrations of compound.

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Screening
Cell-based assays
live-cell imaging
Cell Migration Assays
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Cell-Based Assays
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Enzyme Assays
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Clinical Research
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Biochemical Assays
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Biology
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Cells and Tissues
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Toxicology
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Cell Invasion & Migration Assays
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Mammalian Cell Culture
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Cytometry
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In vitro Disease Models
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Clinical Laboratory Services
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Pharmacology & Toxicology
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Cell Death Assays
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Hit Identification
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Drug Discovery & Development
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Specialized Cell-Based Assays
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Biochemistry & Molecular Biology
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Drug Discovery
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Clinical Microbiology, Virology and Parasitology
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Hydrolase Assays
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