Vhzt89h9qmcmisrsre1b kempbio

Kempbio, Inc.

Frederick, Maryland, US

Kempbio Inc., specializes in contract services and products related to eukaryotic gene expression and purification. Our staff has been producing research grade proteins for the biopharmaceutical industry using the baculovirus expression vector system and mammalian transient expression since 1992 when we were known as Kemp Biotechnologies, Inc. (KBI). Kempbio was founded in 2009 and while we are a smaller company than KBI we still maintain the expertise and depth of knowledge that has helped us succeed where others have failed. Simple Screen™ is Kempbio’s first product offering and is the first complete kit for mammalian gene expression that eliminates the need to amplify and carry the host cell line for transient gene expressions. Our 100L working volume single-use bioreactor reduces the time for completion of protein expression projects and provides an economical platform for pre-GMP expression protocol development.

Kempbio, Inc. has not listed any services.

Ask A Question Find what you're looking for? If not, you can ask this lab a question:
Monoclonal Antibody (mAb) Production
Price on request

Kempbio, Inc. routinely produces monoclonal antibodies from hybridoma and recombinant cell culture systems. We can deliver between 10 milligrams and 30 grams of purified antibody with specifications to meet your application. Our proprietary endotoxin removal step keeps levels well below 1 EU/mg even in the largest scale... Show more »

Kempbio, Inc. routinely produces monoclonal antibodies from hybridoma and recombinant cell culture systems. We can deliver between 10 milligrams and 30 grams of purified antibody with specifications to meet your application. Our proprietary endotoxin removal step keeps levels well below 1 EU/mg even in the largest scale processes. We are experts at detecting and removing aggregates from the final product keeping levels below 5% of the total immunoglobulin. IgG is produced from expression vectors using PEI- mediated transient transfection proteins in suspension cultures of HEK-293 or CHO-S cells at the 1-liter to 100-liter scale. Immunoglobulins are also produced in HEK-293 and CHO-S cells using BacMam mediated transduction of the cells. This method has recently yielded greater than 150 milligrams per liter of purified rIgG from HEK-293 cells transduced at the 10-liter scale.

Typical Services Provided:

  • Gene Synthesis
  • Low endotoxin plasmid production and purification (1-100 mg)
  • Expression optimization
  • HEK-293 or CHO-S host cells
  • Serum-free expression conditions
  • Bioreactor Volumes from 2-L to 100-L
  • PEI-Mediated or BacMam mediated Transfection/Transduction
  • One and two plasmid/vector systems
  • Purification of 10 milligrams to 20 grams of purified IgG

Immunoglobulins Expressed and Purified:

  • Humanized Monoclonal IgG
  • Chimeric antibodies
  • Monoclonal IgG from ascites fluid
  • Peptibodies
  • Bispecific antibodies
  • FAB fragments
« Show less
Baculovirus Titering Service
Price on request

Kempbio determines the infectious titer of baculovirus particles in various types of samples using the standard plaque assay and/or the Virocyt Virus Counter. Plaque assay titers require a week for results to be finalized while titers are available using the Virus Counter in less than 24 hours from the receipt of your sample. By... Show more »

Kempbio determines the infectious titer of baculovirus particles in various types of samples using the standard plaque assay and/or the Virocyt Virus Counter. Plaque assay titers require a week for results to be finalized while titers are available using the Virus Counter in less than 24 hours from the receipt of your sample. By comparing results obtained with these two methods we have developed a numerical factor that converts the virus-particle count obtained with the Virocyt counter to plaque forming units.

For the plaque assay, plates of Sf9 cells are exposed to dilutions of the virus sample and covered with an agarose layer. The plates are incubated for one week at 27ºC and stained with a neutral red dye. The titer of the virus is determined from the mean of two wells at two selected dilutions of virus. The results are presented as pfu/mL HTS.

For the rapid Virocyt Virus Counter method samples are diluted and stained with a combination dye containing fluorescent tags for protein and nucleic acid. The counter detects particles that demonstrate coincident signals from the protein and nucleic acid probes above a certain threshold value. These coincident signals register as a virus particle. In addition to baculovirus this method has been used to successfully determine the titer of various other types of viruses including influenza.

« Show less
BacMam Protein Expression
Price on request

BacMam recombinant baculoviruses are easily amplified in insect cells and efficiently transduce a wide variety of mammalian cell lines. We have developed a robust and scalable protein expression platform based on the BacMam system. We currently offer a complete line of services including production, purification and... Show more »

BacMam recombinant baculoviruses are easily amplified in insect cells and efficiently transduce a wide variety of mammalian cell lines. We have developed a robust and scalable protein expression platform based on the BacMam system. We currently offer a complete line of services including production, purification and characterization of recombinant BacMam baculovirus clones in addition to process development and scale-up for protein expression. Culture volumes from 1 L to 100 liters are available using serum-free media formulations and HEK-293 or CHO-S host cell lines. Optimization of protein expression is accomplished using titration experiments to determine the optimal virus to cell infection ratio and time of harvest. Cell lysate production, supernatant concentration and protein purification services are available.

Typical Services Provided:

  • Gene Synthesis
  • Virus production
  • Clone Isolation and Screening
  • High-Titer Stocks
  • Virus Banking
  • Time Course/MOI
  • Virus Production and Characterization
  • Bioreactor Volumes from 2-L to 100-L
  • HEK-293 and CHO-S Host Cell Lines

Proteins Expressed and Purified:

  • Monoclonal Antibodies
  • Viral Glycoproteins
  • Membrane Proteins
  • Virus-like Particles (VLPs)
  • Enzymes
« Show less
Baculovirus Protein Expression
Price on request

We offer a complete line of services related to the expression of recombinant proteins utilizing the baculovirus expression vector system. Services include production, purification and characterization of recombinant baculovirus clones in addition to process development and scale-up for protein expression. Culture volumes from 1 L... Show more »

We offer a complete line of services related to the expression of recombinant proteins utilizing the baculovirus expression vector system. Services include production, purification and characterization of recombinant baculovirus clones in addition to process development and scale-up for protein expression. Culture volumes from 1 L to 100 liters are available using serum-free media formulations. Optimization of protein expression is accomplished using titration experiments to determine the optimal virus to cell infection ratio and time of harvest. Cell lysate production, supernatant concentration and protein purification services are available.

Typical Services Provided:

  • Gene Synthesis
  • Transfection and Co-transfection
  • Clone Isolation and Screening
  • High-Titer Stocks
  • BIIC/TIPS Virus Banking
  • Time Course/MOI
  • Virus Production and Characterization
  • Bioreactor Volumes from 2-L to 100-L
  • Rapid Expression Protocols
  • High-Throughput Expression

Proteins Expressed and Purified:

  • Viral Glycoproteins
  • Membrane Proteins
  • VLPs
  • Enzymes
« Show less
Protein Expression in Mammalian Cells
Mammalian cell based recombinant protein production
Price on request

Kempbio, Inc. has over 15 years of experience with the transient expression of recombinant proteins in suspension cultures of mammalian host cells at the 1-liter to 100-liter scale. We produce our own proprietary formulation of PEI which allows us to offer exceptional expression results at a reasonable cost. Transfections utilize... Show more »

Kempbio, Inc. has over 15 years of experience with the transient expression of recombinant proteins in suspension cultures of mammalian host cells at the 1-liter to 100-liter scale. We produce our own proprietary formulation of PEI which allows us to offer exceptional expression results at a reasonable cost. Transfections utilize either HEK-293 or CHO-S cells as the host cell for expression and both systems are scalable from 1-liter to 100 liters and above.

Typical Services Provided:

  • Gene Synthesis
  • Low Endotoxin Plasmid Production and Purification (1-100 mg)
  • Expression Optimization
  • HEK-293 or CHO-S Host Cells
  • Serum-free Expression
  • Bioreactor Volumes from 2-L to 100-L
  • Rapid Expression Protocols
  • High-Throughput Expression

Proteins Expressed and Purified:

  • Monoclonal Antibodies
  • Viral Glycoproteins (filoviral)
  • Influenza VLPs
  • Protease Inhibitors
  • Membrane Proteins
« Show less
Biology
Price on request
Request a quote for more information about this service.
Biochemistry & Molecular Biology
Price on request
Request a quote for more information about this service.
Protein Services
Price on request
Request a quote for more information about this service.
Protein Expression
Price on request
Request a quote for more information about this service.
Antibody Services
Price on request
Request a quote for more information about this service.
2018-01-24 13:57:18 -1100

Net Promoter Score of 10 received for BacMam Protein Expression.

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2017-11-01 07:35:39 -1100

Net Promoter Score of 9 received for BacMam Protein Expression.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 8.
2017-10-18 09:57:35 -1100

Net Promoter Score of 10 received for BacMam Protein Expression.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.

Be the first to endorse Kempbio, Inc.

Endorse this lab