O1ysntn7syqirohesuyi kemp proteins logo rgb color

Kemp Proteins

13 Orders Completed
Frederick, Maryland, US

About Kemp Proteins

Kemp Proteins (formerly Kempbio) is a leading provider of gene-to-protein bioservices. Over our 20-year history, our protein problem-solvers have completed more than 20,000 projects and optimized protein-production platforms for gene-to-protein discovery through multigram repeat productions. Kemp... Show more »

Kemp Proteins (formerly Kempbio) is a leading provider of gene-to-protein bioservices. Over our 20-year history, our protein problem-solvers have completed more than 20,000 projects and optimized protein-production platforms for gene-to-protein discovery through multigram repeat productions. Kemp Proteins services include protein expression—mammalian (transient and stable transfection), BacMam, insect, and E. coli platforms—as well as hybridoma-to-recombinant conversion, diagnostic/pre-GMP biomanufacturing, and a range of value-added services, including OEM vialing, lyophilization, stability studies, cell banking, vial labeling, and purification. Kemp’s team of protein problem-solvers has delivered best-in-class services that optimize productivity and mitigate risk for life sciences innovators developing protein-based products, including human and veterinary biopharmaceuticals, vaccines, and diagnostics. Kemp Proteins is a Six.02 Bioservices member company.

Show less

Our Services (19)


ic

Bacterial Protein Expression

Price on request

E. coli Protein Expression Services

If complex post-translation modifications are not required for proper folding and function, bacterial expression is an economical way to produce recombinant proteins.

Kemp Proteins provides protein expression and plasmid production services using numerous... Show more »

E. coli Protein Expression Services

If complex post-translation modifications are not required for proper folding and function, bacterial expression is an economical way to produce recombinant proteins.

Kemp Proteins provides protein expression and plasmid production services using numerous commercially available strains of E. coli cells. We produce recombinant proteins at scales that range from 50 mL to 10 liters using shake flasks and stirred-tank fermenters. We offer gene-to-protein services to produce HIV proteins, scFv, enzymes, and mitochondrial and channel proteins. Our team has developed stable isotope labeling protocols to provide labeled protein for NMR studies. We use affinity, ion-exchange, and size exclusion procedures for protein purification. Proteins are dialyzed into PBS or a buffer designated by the client.

Kemp Proteins offers plasmid production to support protein expression using mammalian transient transfection protocols. We produce and purify low-endotoxin, high-purity plasmid in quantities that support expression in the 1-100 liter range (1-100 mg plasmid). Each batch of purified plasmid is tested for endotoxin and examined on agarose gels for purity and supercoiled state.

Please note: To facilitate affinity purification and/or solubility, most antigens produced in bacteria are fused to a tag.

E. Coli Cell Expression Platforms: scalable from 1 liter to 100 liters and above

E. coli
C. Glutamucun
Our service offerings include:

construct design
fusion partner and affinity tag selection
gene synthesis and codon optimization
subcloning, plasmid isolation, and sequence verification
expression and purification

Show less

ic

Monoclonal Antibody (mAb) Production

Price on request

Kemp Proteins has a long history of producing monoclonal antibodies—both IgG and IgM—from a variety of hybridoma cell lines, including mouse, rat, guinea pig, rabbit, and human. We deliver milligram to gram quantities of low-endotoxin and purified antibody to customer specifications, supported by quality... Show more »

Kemp Proteins has a long history of producing monoclonal antibodies—both IgG and IgM—from a variety of hybridoma cell lines, including mouse, rat, guinea pig, rabbit, and human. We deliver milligram to gram quantities of low-endotoxin and purified antibody to customer specifications, supported by quality documentation (including a Certificate of Analysis).

Kemp Proteins is flexible in production scale (mg to gm quantities) and, once we have assessed the hybridoma, we guarantee production output. When possible, we transfer hybridoma production into serum-free media to facilitate purification and low endotoxin levels. We offer several production platforms appropriate to client needs and quantity, including single-use bioreactors (including Wave), spinner flasks, stirred tank, membrane flasks, and roller bottles.

Hybridoma Source: We accept hybridoma cell lines directly from clients or from third-party sources, or we can develop hybridoma cell lines for the project. If the hybridoma cell line is sourced from the client or from a third party, we require:

  • Mycoplasma-negative confirmation
  • Species of origin
  • Known productivity levels
  • Known required media components
  • Antibody isotype
  • Two vials of frozen cells

Antibody Purification: Our purification group provides both fully automated (BioRad NGC & GE AKTAPilot) and manual purification techniques performed in an ISO 7 / Class 10,000 environment. Our team is experienced with most standard purification media, including Protein A, Protein G, Protein A/G, and LigaTrap. Final buffer requirements are specified by the client.

 Quality Control and Quality Assurance: For standard hybridoma productions, we routinely perform:

  • Protein concentration / total antibody
  • Antibody isotype
  • pH/ buffer type
  • Purity
  • Aggregation
  • Endotoxin levels

In addition, we consider other quality control assay methods requested by clients—we have qualified various client-driven QC methods and have provided the data as part of the release criteria.

Final Product: We store final product in a cold room facility with generator back-up until it is shipped. We package and ship (or drop-ship) final product according to client specifications.

Value-Added Processes: Kemp Proteins offers the following value-added services:

  • Hybridoma Development
  • Hybridoma-to-Recombinant Antibody Conversion
  • Antibody Characterization (including OctetRed96 affinity mapping)
  • Cell Banking
  • Subcloning and Clonality Assurance
  • OEM Vialing / Plating and Labeling
  • Stability Studies
Show less

ic

Recombinant Conversion

Price on request

Kemp Proteins offers custom conversion services for customers that have hybridoma clones they would like to convert to recombinant antibodies.

We offer a complete line of hybridoma-to-recombinant conversion services, including sequencing, cloning, verification, expression, purification, and production.

When clients need a stable... Show more »

Kemp Proteins offers custom conversion services for customers that have hybridoma clones they would like to convert to recombinant antibodies.

We offer a complete line of hybridoma-to-recombinant conversion services, including sequencing, cloning, verification, expression, purification, and production.

When clients need a stable supply of important antibodies, they decide to immortalize the antibody supply with a recombinant conversion. It is a great solution for consistent large-scale productions.

Use our quick quote form to request a quote.

Clients request hybridoma-to-recombinant conversion to resolve issues with problem hybridomas, such as:

• Require class switch
• Unstable hybridoma
• Mycoplasma contamination
• Low expression level
• Lost clone in freezer

Show less

ic

Baculovirus Titering Service

Price on request

Kemp Proteins determines the infectious titer of baculovirus particles in various types of samples using the standard plaque assay and/or the Virocyt Virus Counter. Plaque assay titers require a week for results to be finalized while titers are available using the Virus Counter in less than 24 hours from the receipt of your... Show more »

Kemp Proteins determines the infectious titer of baculovirus particles in various types of samples using the standard plaque assay and/or the Virocyt Virus Counter. Plaque assay titers require a week for results to be finalized while titers are available using the Virus Counter in less than 24 hours from the receipt of your sample. By comparing results obtained with these two methods we have developed a numerical factor that converts the virus-particle count obtained with the Virocyt counter to plaque forming units. For the plaque assay, plates of Sf9 cells are exposed to dilutions of the virus sample and covered with an agarose layer. The plates are incubated for one week at 27ºC and stained with a neutral red dye. The titer of the virus is determined from the mean of two wells at two selected dilutions of virus. The results are presented as pfu/mL HTS. For the rapid Virocyt Virus Counter method samples are diluted and stained with a combination dye containing fluorescent tags for protein and nucleic acid. The counter detects particles that demonstrate coincident signals from the protein and nucleic acid probes above a certain threshold value. These coincident signals register as a virus particle. In addition to baculovirus this method has been used to successfully determine the titer of various other types of viruses including influenza.

Show less

ic

BacMam Protein Expression

Price on request

Kemp Proteins has developed a robust and scalable protein expression platform for BacMam-mediated transductions. We offer a complete line of services, including production, purification, and characterization of recombinant BacMam baculovirus clones as well as process development and scale-up for protein expression.

We... Show more »

Kemp Proteins has developed a robust and scalable protein expression platform for BacMam-mediated transductions. We offer a complete line of services, including production, purification, and characterization of recombinant BacMam baculovirus clones as well as process development and scale-up for protein expression.

We optimize BacMam protein expression using titration experiments to determine the optimal virus-to-cell infection ratio and time of harvest.

Cell lysate production, supernatant concentration, and protein purification services are available.

Multi-host transfection platforms: scalable from 1 liter to 100 liters and above

Mammalian:

  • CHO (chinese hamster ovary cell line)
  • HEK-293 (human embryonic kidney cell line)

Our service offerings include:

  • Gene Synthesis
  • Virus production
  • Clone Isolation and Screening
  • High-Titer Stocks
  • Virus Banking
  • Time Course/MOI
  • Virus Production and Characterization
  • Bioreactor Volumes from 2-L to 100-L
  • HEK-293 and CHO-S Host Cell Lines
     

We express and purify the following proteins:

  • Monoclonal Antibodies
  • Viral Glycoproteins
  • Membrane Proteins
  • Virus-like Particles (VLPs)
  • Enzymes
Show less

ic

Baculovirus/Insect Cell Protein Expression

Price on request

Kemp Proteins’ expertise in the baculovirus expression system (BEVS) is well known in the industry, and our team has completed thousands of baculovirus protein expression projects. Over nearly three decades, we have developed robust protocols for the creation of baculovirus constructs, the characterization of expression... Show more »

Kemp Proteins’ expertise in the baculovirus expression system (BEVS) is well known in the industry, and our team has completed thousands of baculovirus protein expression projects. Over nearly three decades, we have developed robust protocols for the creation of baculovirus constructs, the characterization of expression conditions, and scaleup of protein expression and subsequent purification.

In addition to generating standard baculovirus high-titer stocks, we routinely produce baculovirus-infected insect cells (BIIC) that are cryopreserved as cell banks for use in various expression protocols. We can use three cell lines for production: Sf9, Sf21, and T.ni. We also offer virus plaque purification services, master virus banks, and working virus bank production.

Kemp Proteins has also developed a proprietary PEI-mediated transient transfection protocol using Sf9 and S2 cells for protein expression in shake flasks and stirred tank bioreactors. One advantage of this approach is the ability to rapidly screen variant constructs prior to production. We have been very successful in using this technology to produce VLPs in Sf9 cells at the 2-liter bioreactor scale.

Using these approaches, we have delivered a range of customer-defined proteins, including many difficult-to-express, novel proteins (never previously expressed) that have required our scientists to be innovative in their approach. This breadth of experience makes our team the choice in the industry for expressing your protein in insect cells. Few other companies have the depth of experience represented by the Kemp Proteins team.

We optimize protein expression using titration experiments to determine the optimal virus-to-cell infection ratio and time of harvest from our multi-liter culture in serum-free media formulations.

Cell lysate production, supernatant concentration, and protein purification services are also available.

Baculovirus cell platforms: scalable from 1 liter to 100 liters and above)Sf9 (Spodoptera frugiperda cell line

  • Sf21 (Spodoptera frugiperda cell line)
  • Ni (Trichoplusia ni cell line)
  • S2 (Drosophila melanogaster cell line)
  • BacMam

Our service offerings include:

  • Gene to Protein
  • Single and Multiple Viral Infections
  • Plaque Purification and Screening
  • High-Titer Stocks
  • BIIC / TIPS Virus Banking
  • Time Course / MOI
  • Virus Production and Characterization
  • Bioreactor Volumes from 2-L to 100-L
  • Rapid Expression Protocols
  • High-Throughput Expression

 We express and purify the following proteins:

  • Viral glycoproteins
  • Membrane proteins
  • VLPs
  • Enzymes
  • Multi-protein complexes
  • Nanoparticles
  • Proteases
  • Membrane proteins / GCPRs (no purification)
  • Extracellular domains (ECDs)
Show less

ic

Antibody Modification

Price on request
Request a quote for more information about this service.

ic

Antigen Expression and Purification

Price on request
Request a quote for more information about this service.

ic

Animal Immunization for Antibody Production

Price on request
Request a quote for more information about this service.

ic

Project Management

Price on request

The Kemp team maintains a very high level of communication and interaction with our clients to ensure that they feel in control of their projects as we focus on hitting quality goals and timelines. Our project management team provides full visibility into project progress through regular milestone-based updates, enabling us to... Show more »

The Kemp team maintains a very high level of communication and interaction with our clients to ensure that they feel in control of their projects as we focus on hitting quality goals and timelines. Our project management team provides full visibility into project progress through regular milestone-based updates, enabling us to anticipate emerging client requirements, make timely adjustments that help keep projects on track, and deploy rapid corrective decision-making when encountering unexpected events.

Show less

ic

Protein Expression Screening and Optimization

Price on request
Request a quote for more information about this service.

ic

Recombinant Protein Expression

Price on request

The Kemp Proteins team has more than two decades of experience and technical expertise with transient and stable expression of recombinant proteins in a variety of mammalian host cells. We have successfully delivered a range of customer-defined proteins, including hundreds of difficult-to-express, novel proteins (never previously... Show more »

The Kemp Proteins team has more than two decades of experience and technical expertise with transient and stable expression of recombinant proteins in a variety of mammalian host cells. We have successfully delivered a range of customer-defined proteins, including hundreds of difficult-to-express, novel proteins (never previously expressed) that have required our scientists to “think outside the box.” This breadth of experience in mammalian expression makes our team the choice in the industry for expressing your protein.

We offer a complete gene-to-protein process using our proprietary formulation of PEI that allows us to offer exceptional expression results at a reasonable cost. In addition, we offer “a la carte” mammalian protein expression services, including test expression, production, purification, and characterization of recombinant mammalian clones as well as process development and scale-up for protein expression.

Our production service includes serum-free, multi-liter-scale that provides post-purification products with low endotoxin levels suitable for animal studies.

We are flexible in our approach to working with you, we provide bi-weekly updates, and at the end of every project, we can provide comprehensive documentation to assist with transfer to GMP production.

Show less

ic

Antibody Purification

Price on request

Our purification team has experience with a wide variety of proteins, including antibodies and nanoparticles. We offer purification services for 1- to 100-liter volumes of expressed protein materials. Using our state-of-the-art automated purification equipment, we offer a range of purification methodologies including affinity,... Show more »

Our purification team has experience with a wide variety of proteins, including antibodies and nanoparticles. We offer purification services for 1- to 100-liter volumes of expressed protein materials. Using our state-of-the-art automated purification equipment, we offer a range of purification methodologies including affinity, ionic, and size exclusion processes to provide polished material with low endotoxin suitable for a variety of uses, including preclinical animal studies.

Show less

ic

Biochemistry & Molecular Biology

Price on request
Request a quote for more information about this service.

ic

Protein Production

Price on request

Kemp Proteins offers multi-liter, low-endotoxin protein manufacturing services that ensure a reliable supply of superior-quality material for preclinical research and diagnostics development. Applying more than 30 years of experience manufacturing well-characterized proteins in multi-expression systems, our seasoned team develops... Show more »

Kemp Proteins offers multi-liter, low-endotoxin protein manufacturing services that ensure a reliable supply of superior-quality material for preclinical research and diagnostics development. Applying more than 30 years of experience manufacturing well-characterized proteins in multi-expression systems, our seasoned team develops customized processes that are reproducible, fully scalable, and facilitate translation to GMP production and/or production of large product yields.

We have helped many biotherapeutic and diagnostic companies develop and scale their candidate targets. Our support and documentation has enabled them to easily transfer our processes through to GMP and IVD production.

Our Service Offerings Include:

  • A wide range of mammalian/BacMam, insect, and E. coli protein expression platforms available for production
  • Scalable equipment, technologies, and robust protocols that enable clients to take their customized processes forward independently
  • Multiple bioreactors
Show less

ic

Biology

Price on request
Request a quote for more information about this service.

ic

Antibody Services

Price on request
Request a quote for more information about this service.

ic

Protein Services

Price on request

Over our 20-year history, our protein problem-solvers have completed more than 20,000 projects and optimized protein-production platforms for gene-to-protein discovery through multigram repeat productions. Kemp Proteins services include protein expression—mammalian (transient and stable transfection), BacMam, insect, and E. coli... Show more »

Over our 20-year history, our protein problem-solvers have completed more than 20,000 projects and optimized protein-production platforms for gene-to-protein discovery through multigram repeat productions. Kemp Proteins services include protein expression—mammalian (transient and stable transfection), BacMam, insect, and E. coli platforms—as well as hybridoma-to-recombinant conversion, diagnostic/pre-GMP biomanufacturing, and a range of value-added services, including OEM vialing, lyophilization, stability studies, cell banking, vial labeling, and purification.

Show less

ic

Protein Expression in Mammalian Cells

Mammalian cell based recombinant protein production
Price on request

The Kemp Proteins team has more than two decades of experience and technical expertise with transient and stable expression of recombinant proteins in a variety of mammalian host cells. We have successfully delivered a range of customer-defined proteins, including hundreds of difficult-to-express, novel proteins (never... Show more »

The Kemp Proteins team has more than two decades of experience and technical expertise with transient and stable expression of recombinant proteins in a variety of mammalian host cells. We have successfully delivered a range of customer-defined proteins, including hundreds of difficult-to-express, novel proteins (never previously expressed) that have required our scientists to “think outside the box.” This breadth of experience in mammalian expression makes our team the choice in the industry for expressing your protein.

We offer a complete gene-to-protein process using our proprietary formulation of PEI that allows us to offer exceptional expression results at a reasonable cost. In addition, we offer “a la carte” mammalian protein expression services, including test expression, production, purification, and characterization of recombinant mammalian clones as well as process development and scale-up for protein expression.

Our production service includes serum-free, multi-liter-scale that provides post-purification products with low endotoxin levels suitable for animal studies.

We are flexible in our approach to working with you, we provide bi-weekly updates, and at the end of every project, we can provide comprehensive documentation to assist with transfer to GMP production.

Mammalian Cell Expression Platforms:

  • CHO
  • CHO-S
  • CHO-DG44
  • CHO-SSI
  • HEK-293
  • Hybridoma cell line
  • NS0

Our service offerings include:

  • Gene to Protein
  • Low Endotoxin Plasmid Production and Purification (1–100 mg)
  • Expression Optimization
  • Transient Expression in HEK-293 or CHO-S Host Cells
  • Serum-Free Expression
  • Bioreactor Volumes from 2-L to 100-L
  • Rapid Expression Protocols
  • High-Throughput Expression

We express and purify the following proteins:

  • Monoclonal antibodies
  • Antibody fragments
  • Bi-specific antibodies
  • VHH
  • Viral glycoproteins
  • Virus-like particles (VLPs)
  • Multi-protein complexes
  • Nanoparticles
  • Proteases
  • Membrane proteins / GCPRs (no purification)
  • Extracellular domains (ECDs)
  • Enzymes
  • Protein hormones
Show less

Not finding what you're looking for?

Get info on this provider's capabilities without requesting a quote.
JC

Joe Corvera

Project Manager
KK

Kara Karabias

Director of Commercial Operations
CK

Kemp Proteins has not received any reviews.

Kemp Proteins has not received any endorsements.