++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Karebay Biochem Core Services: cGMP Peptides and cGMP Small Molecules Simple and Modified Oligonucleotides ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ There is nothing more satisfying than getting acknowledgement from our clients. We got just such good news yesterday. One of our many happy customers recently published a article in Nature in which he referenced KareBay Biochem Inc. for the AMK peptide synthesis. As a token of deep appreciation, he also thanked us for providing such excellent reagents at such good prices with speedy delivery. All we want to say is that it is you, our happy customers from around the world, that we need to thank and show our deepest appreciation. Without you, there would not be KareBay Biochem Inc. Therefore, please accept our sincere thanks for working with us and allowing us to instill our contribution into your scientific endeavors. We will certainly carry on our good work and provide more and more high-caliber products for years to come. You could find Nature article here: http://www.nature.com/nature/journal/v532/n7597/full/nature17402.html KareBay™ Biochem is a globally operated company dedicated to the innovation and manufacture of biochemical products and services. KareBay Biochem provides complete strategic solutions to simplify your laboratory experience, whether it be basic biological research, pharmaceutical drug discovery, drug development or manufacturing. Our team encompasses experienced chemists and biologists who provide all necessary services, from custom chemical synthesis to biological production. The KareBay team has devised comprehensive solutions to meet your specific requirements in organic synthesis, macromolecule production and preclinical assay development. Committed to providing the greatest satisfaction, we track each milestone from precursor synthesis to quality analysis and assessment to ensure the highest success rate and a quick turnaround time. KarebayBiochem is prepared to be your partner and help you achieve sustainable success in your biotechnology or pharmaceutical enterprise.
“We have recently published a Nature paper in which we referenced your company for the AMK peptide synthesis. Thank you for providing excellent reagents!”
Custom peptide synthesis
KareBayTM Biochem is a global leading biotech CMO focused on providing customers with custom & catalog peptides, GMP/GLP grade peptides, and peptide library for research and pre-clinical applications. KareBay has a seasoned manufacture team, and our quality department performs the rigorous quality management to assure the conformity of the product and the process. KareBay’s scientists are experienced in peptide manufacturing and research, such as solution phase/solid phase synthesis, custom peptide synthesis, peptide combinatorial chemistry, peptide modifications, peptidomimetics, enzyme substrate design and synthesis, unusual amino acid design and synthesis, and other proteomic entities.
KareBay has full peptide therapeutics development capacity (Pharmability) under its state of the art process facilities, supported by its formulation optimization platform and cGMP quality system. KareBay has already developed broad experience in pharmaceutical peptide field, which includes than 60 peptide APIs and Inhibitors at various stage of trials (See Product list). KareBay can not only partner with big pharma to develop new peptide therapeutics and API, but also can provide biotech enterprises and academic institutes with strategic technical collaboration in their peptide research projects. With its experienced manufacture management team, KareBay has a great advantage in cost, quality, and lead-time control over its competitors due to its technical proficiencies, efficient integrated operation systems (Business development, Customer support, Quality, Production, and Supply-chain). KareBay welcomes all customers and partners from universities, research organizations, pharmaceuticals, biotech and everyone in need of peptide research and custom peptide synthesis services.
Standard peptide synthesis
Scientists at KareBay have developed a reliable synthesis platform and purification system to ensure a success rate of over 95%, even for peptides as long as 180 amino acids or with complex modifications like triple-bisulfide bridges or Fmoc labelling.
In order to provide the highest satisfaction, KareBay offers three packages to help you pick the best-fit solution for your biological or preclinical research.
GMP/ GMP peptide manufacturing
KareBayTM Biochem has constructed a GMP-grade facility so that our team of skilled experts can bring you the highest quality products in peptide manufacturing. KareBay’s GMP Peptide Manufacturing specializes in process development and scale-up production. Our team assists clients through every step of the development process and our experts will carry out the most effective routing from synthesis to purification.
Scale-Up (Contract Manufacturing) Peptides: KareBay is ready to build long-term partnerships with clients who are looking for a reliable partner in scale-up API production. Multiple size reactors support manufacturing capacity from gram to kilogram scales. Key equipment such as gas chromatography (GC), analytical HPLC, small and large preparative HPLC systems and MS monitors, as well as are employed to assess each step of manufacturing with complete GMP grade certificates.
KareBay Bio™ provides various high-quality oligonucleotides in large quantities only with good raw materials, advanced biolytic synthesizer, optimized process and professional scientists. Quality of the oligonucleotides are all managed and guaranteed with strict quality control system. For any specific requirements, we also offer custom DNA oligonucleotide modification services. The oligonucleotides can be applied not only in gene synthesis, but also in biochips, sequencing, genome amplification and large-scale gene cloning and expressions.
All the fluorescent modification oligos are purified with HPLC. Please note in order if the HPLC+PAGE purification is required.
Our skilled and experienced team of organic chemists can provide the custom organic componds synthesis of almost any small molecule compound, including substrates, inhibitors and activators. We offer different fluorescent dyes (such as CY3, CY5, CY5.5, Cy7.5) and prepare intermediates, reference compounds, starting materials and derivatives of lead compounds. All organic compounds produced in our facility are made to order in scales ranging from milligram to multi-gram quantities.
All custom organic compounds synthesis requests we receive are carefully evaluated to determine whether we are able to competitively meet the highest standards for your project. This process usually takes no longer than 48 hours.
Simply provide the chemical name, structure or CAS number and our experienced scientific team will design the organic synthetic route and ship the product with your desired purity level. You can also provide us with any known literature methods and/or your own existing synthetic route, including experimental details, reaction times, temperatures, solvents and concentrations, work-up procedures, purification methods, yields and any analytical data. The more information you provide us with, the shorter the turnaround time and the more competitively priced the project will be. Contact us for much more information about our contract organic compound synthesis service.
KareBayTM Biochem has developed and optimized a highly automatic controlled synthesis system and is capable of accurately synthesizing a libraries of thousands of peptides. A comprehensive list of modification options is ready so that you may choose which products are right for you. Each peptide comes accompanied by complete quality reports.
KareBay offers two packages to target your project needs: Rush Screening Package and High Throughput Package. KareBay’s skilled PhD level technology support team will closely work with you to optimize your library and find which package best suits your needs.
KareBay™ Biochem provides a comprehensive protein expression services and protein purification using both mammalian cells and bacteria systems. Generally, mammalian expression systems are suitable for glycosylated protein expression. Mammalian cells are widely used in the production of recombinant proteins and antibodies. Bacteria expression systems are preferred for non-glycosylated proteins because they are simpler, quicker and less expensive. Recombinant proteins are mainly used for biochemical and functional assays, antigen generation and a variety of other applications. We also offer a full service for protein purification. Our objective is to ensure efficient and high quality protein production for your R&D needs.
In addition, we perform a series of protein characterization and protein-based assays to meet your needs. It is worth mentioning that various enzyme activity-based assays allow high throughput drug screening to be performed. For example, we can provide you with kinase and protease activity assay services, as well as related biochemicals such as inhibitors, activators and substrates.
Once your order is confirmed, Karebay Biochem will initiate your protein expression and protein purification project using a plasmid obtained either from gene synthesis or from your own laboratory. The desired gene will be subcloned into an expression vector to express the targeted protein in bacteria. One liter of culture will be grown using our optimized protocol. Proteins can be purified using any common epitope tag of interest (FLAG, HIS, V5, c-myc, and HA).
Cloning into bacterial expression vector Amplification of the gene of interest and subcloning it into a bacterial expression vector; Verification of the authenticity of the Subcloned gene by restriction enzyme digest and sequencing; Maxi-prep of the recombinant vector DNA.
Generation and identification of bacterial colonies; Expressing target proteins. Transformation of recombinant constructs into high efficiency expression bacterial strain; Mini-induction to over-express the target protein; Test for the expression of the recombinant protein by western blot with anti-tag antibody (customer must provide appropriate antibodies) if desired.
Large-Scale Culture One liters of bacterial culture will be induced with IPTG and harvested for protein purification.
Purification Protein purification using Ni-NTA (for 6xHIS-tagged proteins) or glutathione (for GST-tagged proteins) beads at either native or denatured conditions.
Total Price per Protein
Bioconjugation is the process of coupling two biomolecules together using a covalent linkage. Common types of bioconjugate chemistry are amine coupling of lysine amino acid residues (typically through amine-reactive succinimidyl esters), sulfhydryl coupling of cysteine residues (via a sulfhydryl-reactive maleimide or haloacetamide), and photochemically initiating free radical reactions, which have a broader reactivity. The product of these reactions is referred to as a bioconjugate.
Common bioconjugation procedures include coupling small molecules, such as biotin or fluorophores, to a protein, or protein-protein conjugations, such as the coupling of an antibody to an enzyme. Other less common molecules used in bioconjugation include oligosaccharides, nucleic acids, carbon nanotubes and synthetic polymers, such as polyethylene glycol (a.k.a. PEG). The KareBay team of chemists is adept in all aspects of the bioconjugation process.
μg to g amounts of plasmid according to your specifications
KareBay™ Biochem provides comprehensive molecular biology services including gene synthesis, cloning, plasmid preparation. Our experienced team uses advanced technologies to rapidly and accurately synthesize any of your target gene sequence. Simply submit the sequence of your target gene. Our professional technical support will work closely with you to analyze and optimize your sequence according to your specific needs. And the target gene will be synthesized and delivered in as little as 10 business days. KareBay welcomes all customers and partners from research organizations, universities, biotech, pharmaceuticals, and everyone in need of molecular biology services.
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Karebay™ Biochem provides high quality IHC services and immunohistochemical staining to pharmaceutical, medical device and academic clients. Our team works carefully to standardize immunohistochemistry processes for down-stream whole slide image quantitation. We have industry leading expertise in the use of dual staining in brightfield immunohistochemistry, and have developed a large number of protocols. By choosing KareBay you will receive the best results for your immunohistochemistry needs.
KareBay™ Biochem is prepared to supply our customers with the professional technical immunoprecipitation services relevant to immunoprecipitation. We can setup a preliminary experiment to explore the optimal sample volume and optimal antibody dilution according to the specific circumstances of the customer’s sample.
Enzyme-linked immunosorbent assay (ELISA) is a biochemical technique used to detect the presence of a specific antigen or antibody in a sample. The ELISA has maintained its popularity for over 40 years due to its high specificity, sensitivity, and reliability – characteristics that customers consistently cite for using the method.
ELISA Services and Test Methods
The direct version of the ELISA assay uses monoclonal antibodies to test for a specific antigen. This form of ELISA testing is used primarily in the immunohistochemical staining of tissues and cells.
Direct ELISA, when compared to other forms of ELISA testing, is performed quickly because only one antibody is employed. This assay can be used to test specific antibody-to-antigen reactions, and helps avoid complications due to cross-reactivity between other antibodies. Disadvantages The primary antibody must be labeled individually, which can be time-consuming and arduous when performing multiple experiments. Also, the signal is not as amplified in direct ELISA, compared with the indirect approach, which can be a disadvantage in some applications involving trace analyte detection.
Indirect ELISA Test
The indirect detection method uses a labeled secondary antibody for detection and is the most popular format for ELISA. The secondary antibody has specificity for the primary antibody. In a sandwich ELISA, it is critical that the secondary antibody is specific for the detection of the primary antibody only (and not the capture antibody) or the assay will not be specific for the antigen. Generally, this is achieved by using capture and primary antibodies from different host species (e.g., mouse IgG and rabbit IgG, respectively). For sandwich assays, it is beneficial to use secondary antibodies that have been cross-adsorbed to remove any antibodies that have affinity for the capture antibody. The most powerful ELISA assay format is the sandwich assay. This type of capture assay is called a “sandwich” assay because the analyte to be measured is bound between two primary antibodies – the capture antibody and the detection antibody. The sandwich format is used because it is sensitive and robust.
Competitive ELISA Test
The competitive ELISA is used to quantify antigen using a competition method. Briefly, the free antigen and antibody are incubated to form antigen-antibody complex and then the complex is added to an antigen-coated surface in the assay plate. The unbound antibody-antigen complex is washed off before adding enzyme-linked secondary antibody against the primary antibody. The substrate is then added and the antigen concentration is subsequently determined by the signal strength elicited by the enzyme-substrate reaction. In this assay, the enzyme-linked secondary antibody competes with the sample antigen, which is associated with the primary antibody.
With a professional ELISA team and state-of-the-art equipment, KareBay™ Biochem will provide you with the highest quality service. It is simple to work with us. You send us your samples, we send you back the test results.
"I chose KareBay for my peptide synthesis for its attractive timelines and costs. I was very satisfied to receive my 10-aa peptide within 3 weeks."
"It was an excellent experience to work with KareBay Biochem on a custom peptide synthesis. The turnaround time was very quick and the pricing for their service is competitive. Communication on the request was also great, with updates provided along the way. Thank you; we look forward to future orders."
"I recently placed an order with Karebay Biochem Inc. and I was very satisfied with their service. I communicated with Jack and he constantly kept me updated about the status of my order and answer the million of questions I had. In addition, there was a confusion with my order and I received an empty package but when I called KareBay they immediately told me not to worry that they would take care of it. I received my chemical the next day. Today I performed a first test with the compound and obtained positive results!"
"Service was great."
"KareBay BioChem Inc provided quality service with fast lead times at a reasonable price. I had a great experience working with this company and would highly suggest them!"
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