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InVivo BioTech Services GmbH

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Hennigsdorf, DE

About InVivo BioTech Services GmbH

InVivo is one of the leading contract manufacturing organizations (CMOs) in Europe, with more than 20 years of experience in mammalian cell culture and protein production.

Founded in 1998, our company has constantly grown over time and became a part of the Bruker Corporation in 2017. We are based in... Show more »

InVivo is one of the leading contract manufacturing organizations (CMOs) in Europe, with more than 20 years of experience in mammalian cell culture and protein production.

Founded in 1998, our company has constantly grown over time and became a part of the Bruker Corporation in 2017. We are based in Hennigsdorf, just outside Berlin.

Rely on our experience!

As a trusted provider of cost-effective outsourcing solutions, InVivo has worked with a large number of satisfied clients worldwide. Companies of all sizes, including small university spin-offs, major research institutes, and biopharmaceutical companies, have taken advantage of our expertise as an independent contract manufacturer.

Our services include:

  • Production of monoclonal antibodies
  • Bacterial protein expression
  • Transient expression of recombinant antibodies and proteins in mammalian systems
  • Hybridoma development
  • Development of stable cell lines (HEK and CHO) for research or pre-clinical use
  • Immunoassay development
  • Etc.

For large-scale transient gene expression in HEK cells, we established our proprietary, highly sophisticated expression system: InVEST. It combines an optimized host cell line, customized cell culture media, efficient large-scale plasmid preparation, superior vector design and a novel polycationic transfection reagent.

Our goal is to deliver individual solutions, exactly tailored to the client’s needs and to create lasting relationships by providing outstanding services and high-quality products to demanding clients. Rely on us for your diagnostic or biomedical outsourcing needs.

We value quality!

InVivo is ISO 9001 certified since 2004 and our antibodies and proteins can be found in many FDA-approved diagnostic kits.

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Our Services (16)


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Hybridoma Sequencing

Price on request
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Recombinant Protein Expression

Price on request

We offer you custom recombinant protein production exactly tailored to your needs. With more than 700 successfully completed projects, InVivo is a well experienced service partner to get you started on your transient expression project.
Using our proprietary system InVEST for large-scale transient gene expression in HEK cells, we... Show more »

We offer you custom recombinant protein production exactly tailored to your needs. With more than 700 successfully completed projects, InVivo is a well experienced service partner to get you started on your transient expression project.
Using our proprietary system InVEST for large-scale transient gene expression in HEK cells, we deliver milligram to gram amounts of high-quality recombinant proteins in only a few weeks.

1) Feasibility Study and Extended Feasibility Study
We conduct a small-scale pilot production in HEK cells to assess the feasibility to express and purify your protein of interest.

Service package includes:
- cDNA synthesis (incl. codon optimization), cloning into InVivo`s proprietary expression vector and endotoxin-free plasmid preparation
- Small-scale transient transfection of HEK cells
- Cultivation of 150 ml culture (Feasibility Study) or 1L culture (Extended Feasibility Study)
- One-step purification
- UV280 nm, CGE or SDS-PAGE

Additional services:
- Up to 100 small-scale (5-20 mg) expression assays in parallel (Target Discovery, Drug Screening, etc.)
- Species and subclass switches
- Protein mutations through amino acid exchanges
- Tag cleavage

2) Recombination protein Production (large scale)
Subsequent to the feasibility study, we offer you our large-scale production service for recombinant proteins. Using this service, you can receive milligram to gram amounts of your recombinant protein with a fixed unit price per mg protein.

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Custom Monoclonal Antibody (mAb) Production

Price on request
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Monoclonal Antibody (mAb) Production

Price on request

In Vivo has more than 20 years of experience in the development, production and purification of monoclonal antibodies (mAB). We processed over 3,500 different hybridoma clones in our lab, generating antibodies of all kinds of species (rat, mouse, human, etc.) and subclasses (IgMs, IgGs).
Using in-vitro production in our... Show more »

In Vivo has more than 20 years of experience in the development, production and purification of monoclonal antibodies (mAB). We processed over 3,500 different hybridoma clones in our lab, generating antibodies of all kinds of species (rat, mouse, human, etc.) and subclasses (IgMs, IgGs).
Using in-vitro production in our proprietary serum-free media, we assure cost-efficient processes with a protein yield up to 500 g through high cell density fermentation (>2 x 10^7 cells per ml).

Quality guaranteed:
- Highest purity
- Aggregate-free
- Endotoxin-free (<1EU/mg)
- No traces of bovine IgGs
- Perfect batch-to-batch uniformity

Depending on the productivity of your cell line and the desired amount of antibody, our prices vary between € 5 and € 55 per mg of purified mAB. We are ready for a test production to provide you with an individualized quote.

Test production includes
- Adaptation to our serum-free media
- Cultivation at 1-liter scale
- Purification with protein-A/G chromatography
- Yield guarantee of 10 mg of purified antibody
- Price: € 999.00

Customers ordering antibodies on a regular basis can take advantage of our Antibodies-On-Demand service.

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Recombinant Antibody Production

Price on request

Transient gene expression (TGE) is the method of choice when it comes to fast production of recombinant antibodies in mammalian systems (HEK and CHO). Using transient expression, you can skip the time-consuming and labor-intensive creation of stable cell lines and receive milligram to gram amounts of your protein of interest... Show more »

Transient gene expression (TGE) is the method of choice when it comes to fast production of recombinant antibodies in mammalian systems (HEK and CHO). Using transient expression, you can skip the time-consuming and labor-intensive creation of stable cell lines and receive milligram to gram amounts of your protein of interest within weeks.

Within years of research and development we established InVEST, our proprietary system for large-scale transient gene expression in HEK cells. InVEST is a highly optimized system which allows an increase in production up to 80-fold compared to standard systems.

InVivo is a well experienced service provider with more than 700 successfully completed TGE projects. We offer you custom recombinant antibody production, exactly tailored to your needs.

Service package includes:
- cDNA synthesis (incl. codon optimization) and cloning into InVivo`s proprietary expression vector
- Endotoxin-free plasmid preparation
- Transient transfection of HEK cells
- One-step purification via protein A affinity chromatography
- QC services: UV280 nm, Capillary Gel Electrophoreses (CGE), analytical SEC
- Guaranteed purity ≥ 90%

Deliverables:
- 40 mg of high-quality recombinant antibodies (IgG)
- LC and HC in synthesis vectors (1-2 μg)
- Certificate of Analysis

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Hybridoma Development

Price on request

InVivo provides custom services for the development of mouse hybridomas and the subsequent production of monoclonal antibodies against your antigen of interest.

  1. Immunization (using proteins, peptides or haptens)
  • Starting material: 5 mg antigen of interest provided by customer (alternatively, the antigen can be produced by... Show more »

InVivo provides custom services for the development of mouse hybridomas and the subsequent production of monoclonal antibodies against your antigen of interest.

  1. Immunization (using proteins, peptides or haptens)
  • Starting material: 5 mg antigen of interest provided by customer (alternatively, the antigen can be produced by InVivo, in E. coli or in HEK/CHO cells via transient expression).
  • Immunization of three Balb/c mice, incl. boosting and bleeding
  • Detection of antibody titer in mouse serum by ELISA and selection of the highest-titer mouse for subsequent cell fusion
  1. Cell Fusion and Screening
  • Generation of hybridoma primary cultures by fusion of spleen cells from the selected mouse with the myeloma cell line SP2/0
  • Screening of primary cultures via ELISA for antibody producers
  • Cell propagation and retesting of antibody titers to find clones
  1. Limited Dilution Cloning, Screening and Characterization
  • Two-step-selection process to identify the two final clones (stable producers), via limited dilution cloning and antibody titer testing by ELISA
  • Cultivation and propagation of the two final clones
  • Isotyping and mycoplasma test
  • Cryoconservation

Deliverables:
- Cryo-stored clones on dry ice (3 vials for each final clone)
- Detailed protocol and documentation

Our standard procedure can be adjusted to individual customer needs.

Additional Services:
- Welcome Offer: Cultivation in 1 L scale. One-Step Purification. Guaranteed yield of 10 mg purified monoclonal antibody.
- Screening for antibody candidates for sandwich ELISA development
- Negative screening to avoid cross-reactions of antibody with unspecific partners

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Animal Immunization for Antibody Production

Price on request

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Request a quote for more information about this service.

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Hybridoma Subcloning

Price on request

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Request a quote for more information about this service.

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Mammalian Cell Culture

Price on request

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Request a quote for more information about this service.

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Antibody Services

Price on request
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Immunoassay Development

Price on request

InVivo is dedicated to the development and production of innovative immunoassay components and kits for research use. Through experience and know-how, we enable fast and more cost-effective solutions for ready-to-use products.
Our quality system is in compliance to ISO 9001.

InVivo is dedicated to the development and production of innovative immunoassay components and kits for research use. Through experience and know-how, we enable fast and more cost-effective solutions for ready-to-use products.
Our quality system is in compliance to ISO 9001.

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Bacterial Protein Expression

Price on request

Choosing bacterial expression systems for the production of recombinant proteins, offers several advantages: A shorter timeline for the entire process from cloning to protein recovery, inexpensive production processes, high protein yields and a high flexibility in scale.

While this expression system is not suitable for every... Show more »

Choosing bacterial expression systems for the production of recombinant proteins, offers several advantages: A shorter timeline for the entire process from cloning to protein recovery, inexpensive production processes, high protein yields and a high flexibility in scale.

While this expression system is not suitable for every project, smaller peptides and recombinant proteins without posttranslational modifications can easily be expressed in E. coli. InVivo`s protein experts are happy to help you choose the right expression system for your application.

For protein expression in E. coli, we offer a huge selection of different culture vessels (2 L shaker flasks to 50 L bioreactors) to ensure that we can meet all your needs. Furthermore, 10 L bioreactors (OD 30-80) are available for high cell density cultivation.

Our services include:

  • cDNA synthesis and cloning into protein expression vector (including codon optimization and attachment of tag (e.g. HIS-tag) for subsequent protein purification)
  • Small scale pilot production to assess the feasibility of expression and purification of your protein of interest (Feasibility Study)
  • Large scale production after successful test production
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Custom Immunoassay Development

Price on request
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Recombinant Antigen Development

Price on request

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Request a quote for more information about this service.

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Plasmid DNA Preparation

Price on request

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Request a quote for more information about this service.

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Antigen Expression and Purification

Price on request

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Request a quote for more information about this service.

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Case-study_Converting-rabbit-hybridoma-into-recombinant-antibodies.pdf

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Flyer_InVivo_Hybridoma development.pdf

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Flyer_InVivo_mAB_10 mg Welcome Offer.pdf

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Flyer_InVivo_Rec AB.pdf

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Flyer_InVivo_Transient Gene Expression via InVEST.pdf

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InVivo_Difficult to Express Proteins.pdf

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InVivo_Expression-of-recombinant-proteins-in-early-and-late-stage-discovery.pdf

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