In-vitro mast cell degranulation assay (96-well format) using peritoneal lavage cells as source of mast cells from rat or mouse.

Human or mouse T cell proliferation assays. Activators: anti-CD3 +/- anti-CD28 in soluble or plate-bound format; Anti-CD3 coated beads. 96-well format.

Our DO11 in-vivo T-cell activation model is a robust mouse model that is relatively quick (3 days) and amenable for evaluation of compounds in-vivo to assess drug effect on T-cell and antigen presenting cell (e.g., dendritic cells) functions. DO11 transgenic mice express the ovalbumin-specific T-cell receptor. Treated mice receive a bolus of ovalbumin protein/peptide injection and plasma cytokine levels are monitored over time. The model is ideal for determining compound MOA or confirming proposed MOA targeting T-cells and/or antigen presenting cells. This service is IACUC supported to ensure proper care and use of animals in research.

The above model is also useful in in-vitro settings to evaluate compound effects for higher throughput.

Human or mouse B-cell activation assays. Activators: anti-IgM, anti-CD40, LPS.

High-throughput 96-well format

White blood cell concentrate of LRS (leukoreduction system) chamber recovered after plateletpheresis procedure. Contains red blood cells, plasma and negligible amount of anticoagulant (ACD-A). Derived from healthy donors whose identity and information will not be provided. Ships overnight by FedEx. Same day courier service to Southern California locations available. Price excludes shipping/delivery cost. Available to US-based customers only. For research use.

Tags: Human Peripheral Blood Mononuclear Cells (PBMCs) , human whole blood, human PBMC

Courier pickup and delivery of precious frozen biological samples in liquid nitrogen dry vapor dewars or in dry-ice. On-demand monitoring report of location and temperature.

Tags: cryo-transport, transport, cryo-port, frozen shipping, dry-ice shipping

High-throughput 96-well format. Brd-U incorporation assessed by Brd-U ELISA. MTS (modified MTT method).

High-throughput 96-well format (colorimetric or fluorescence based). Flow cytometry analysis is also available.

Cells and tissues are provided fresh or frozen. Courier same day delivery available to Los Angeles, San Diego area. Otherwise, FedEx next day AM air service is the alternative delivery method.

Human (cells are derived from normal healthy blood donors).
1. PBMCs
2. Whole blood (in EDTA lavender collection tube)

Rat or Mouse
1. Whole blood (in EDTA)
2. Splenocytes
3. Organs (as per request)
4. Tissues (as per reques)

High-throughput 96-well format (colorimetric or fluorescence based). Flow cytometry analysis is also available.

Blood derived (magnetically purified) or Bone-marrow differentiated Dendritic Cells. Purity analysis by flow cytometry.

Blood derived (magnetically purified) or monocyte differentiated dendritic cells. Purity analysis by flow cytometry.

Purified primary sympathetic neurons from superior cervical ganglia (SCG) of newborn (PO-P1) rat pups

Kupffer Cells from mouse liver. Enzymatically dissociated and magnetically purified. Validation by flow cytometry.

Magnetic-based purification and/or cell sorting (FACS). Freshly purified cells from blood or tissues from human, rat and mouse. Cells from tissues e.g., spleen, liver, heart, lungs, neural tissues, tumors, brain, thymus and lymph nodes are isolated using an automated dissociator employing gentle preparation of single cells. Sterile sample handling, same day isolation and delivery are available at request.

Xenograft subcutaneous tumor models in immunodeficient mice (e.g., SCID, Nude)

Our 24/7 secured facility is attended by a skilled staff with many years of experience in proper handling of research animals. We are committed to the proper care and use of animals in research. In addition to having an IACUC (institutional animal care and use committee), we are approved by the California Department of Public Health to work exclusively with laboratory rats and mice.

High-throughput 96-well format

Cell proliferation, viability or migration assays
Cell activation, stimulation or co-culture assays
Flow cytometry (FACS) based assays

Our 24/7 secured facility is attended by a skilled staff with many years of experience in proper handling of research animals. We are committed to the proper care and use of animals in research. In addition to having an IACUC (institutional animal care and use committee), we are approved by the California Department of Public Health to work exclusively with laboratory rats and mice.

Please contact us for additional information on:
1) Mouse cancer models (syngeneic and xenograft tumor models)
2) Inflammation disease models (e.g., mouse CIA [collagen-induced arthritis model])
3) Obesity mouse model
4) And others.

Diet-induced obese (DIO) mouse model. Summary: C57BL/6 obese mice are allowed to acclimate over a period of 2 weeks while on a high-fat diet and enrolled into treatment groups when body weights stabilize and/or exhibit an upward trend. Mice are kept on a high-fat diet during treatment and weight recorded. Treatment route will vary as per client request. Terminal procedures performed (e.g., blood (plasma) collection, tissue harvest) will vary as per client request. This service is IACUC supported to ensure proper care and use of animals in research.

Our mouse collagen-induced arthritis (cia) is a robust and reliable model for efficacy testing of compounds. Male or female DBA/1 mice are immunized with type II collagen emulsified in Complete Freund's Adjuvant (CFA) (Day 1-20) and with type II collagen emulsified in Incomplete Freund's Adjuvant (IFA) three weeks later (Day 21). Prophylactic, semi-therapeutic or therapeutic treatment may be administered by oral gavage, intravenous injection, subcutaneous, intraperitoneal injection and/or implant with an osmotic pump. Controls available inlcude: anti-TNF biologics and/or dexamethasone. Clinical scores include a) Paw scores, b) Hind-paw thickness and c) Body weight. Independent histopathological analysis of joints is available at request and provided by a certified pathologist. This service is IACUC supported to ensure proper care and use of animals in research.

Obtain freshly isolated peritoneal cells from rat and mice by lavage method. Cost of animals are separate and based on cost associated with vendor price and necessary housing/husbandry.

Tissues and organs harvest from Mouse (For rats, please submit request for quote).

Minimum 10 mice - e.g., common animal strains C57BL/6, Balb/c
Includes 2 organs harvested - e.g., spleen and blood
Additional organ - add $10/animal

Organs usually shipped in culture medium or preferred buffer overnight by FedEx in ice-pack.

We provide cytokine quantification by ELISA, EIA or flow cytometry (e.g., intracellular). We also offer multiplex testing to measure multiple Cytokines, Chemokines and Growth Factors from limited sample volumes of serum, plasma or supernatant.

Vaccine development in mice (cellular & antibody response)

We employ industry-standard cell assay and cell isolation technology with many years of cell-based assay experience to provide independent/unbiased validation and testing of life science products for use in cell-based assays and related applications. Services are non-GLP/GMP and offer a cost-effective step toward GLP/GMP testing. Services come with its exclusive warranty of repeat service or money-back guarantee.

Service Includes (not limited to):
- Confidential and rapid initiation
- Testing in custom or standard cell assays
- Side-by-side comparison with standard control
- Independent review by expert consultant (option)
- Comprehensive study report and post-study support

High-throughput 96-well format

• Cell proliferation, viability or migration assays
  
• Cell activation, stimulation or co-culture assays
  
• Flow cytometry (FACS) based assays

• Immunogenicity testing of therapeutic proteins in pre- or clinical samples in mice and rats assessing cellular & antibody immune responses.

Our flow cytometry services offer cellular analysis and high-speed cell separation by fluorescence-activated cell sorting (FACS) enhanced by magnetic bead-based pre-isolation to insure purity and high efficiency. Same day pickup/delivery is available to Los Angeles, San Diego, and Inland Empire customers.

• Intracellular and surface marker analysis
  
• 4-color parameter & cell-cycle analysis
  
• Cell sorting by BD FACSAria (opt: sterile sort)
  
• Pre-diagnostic clinical evaluation (non-CLIA)
  
• Rapid initiation and confidential
  
• Most BSL-1 samples accepted