The Donald Danforth Plant Science Center's Integrated Microscopy Facility furnishes excellent instrumentation for cellular imaging - we encourage you to contact us for your imaging needs. The facility was established in 2001 as part of the Donald Danforth Plant Science Center, the country's premiere plant science research institute. The facility’s instrument for live cell imaging is a Leica SP8-X confocal microscope equipped with white light laser, resonance scanner and HyD detectors. In addition to this we have other fluorescence microscopes to meet our users' needs. The facility has a workstation for confocal image processing, equipped with Bitplane Imaris (for 3D and 4D image reconstruction, tracking, and colocalization analysis) and SVI’s Huygens deconvolution software (for improved signal:noise and resolution). Confocal microscopy requires a solid background in understanding how to optimally acquire image data and prepare it for publication. To that end, the facility offers comprehensive five day confocal microscopy workshops in January and July. The facility's electron microscopes include a LEO 912 AB energy-filter transmission electron microscope (TEM) and a Hitachi TM-1000 tabletop SEM. We have state-of-the-art specimen preparation for TEM: ultra-rapid freezing done using a Bal-Tec high pressure freezer. We also have the ancillary equipment required for transmission electron microscopy. The facility director R.H. Berg has 40 year's worth of experience in the study of cell biology and he serves as a resource for users in designing and implementing their imaging experiments. Services are provided to any interested party and are designed with convenience in mind for the client. For those wanting to use the instrument themselves, qualifying for the self-service fee schedule, this can be done after training by IMF staff. On the other hand, full service is also available, putting the work in the hand of our experienced staff.
Our transmission electron microscope is a LEO 912 AB energy filter TEM, operating at 120 Kv and equipped with a 2k X 2k slow scanned CCD camera. The energy filter permits EELS elemental analysis of thin sectioned samples. Our ultramicrotome is a Leica Ultracut UCT.
We have extensive experience in high pressure freezing samples for electron microscopy and have established automated workflow for freeze substitution of frozen samples. Our high pressure freezer is a Baltic HPM 010, and our freeze substitution robot is a Leica EM AFS2. Please contact us for detailed pricing.
We have two SEMs. Our Hitachi TM1000 tabletop SEM (with BSE detector) permits imaging of fresh samples, circumventing the need for specimen preparation. Our Hitachi S2600H (with SE detector) is for imaging of conventionally prepared specimens. Ancillary equipment for specimen preparation include a critical point dryer and sputter coater.
Our Leica SP8-X confocal microscope is fully equipped, including a white light laser (200 laser lines), resonance scanner (8,000 lines/second), and three of the five detectors are Leica's hybrid HyD detectors with high speed and sensitivity. We offer full service and self service access. This instrument is optimized for 3D and 4D (time lapse) imaging. The instrument is well equipped for spectral imaging.
Our image processing workstation offers software for deconvolution (SVI's Huygens) and 3D and 4D reconstructions (Bitplane Imaris) of fluorescence image datasets. We provide training in the use of this software.
We have built a light sheet microscope based on the openSPIM platform, for imaging large samples rapidly and for time lapse imaging of sensitive samples.
We have both a fluorescence stereoscope and an epifluorescence widefield microscope.
We offer a four day confocal microscopy short course that furnishes a solid background in all aspects of light microscopy and gives three hands-on sessions to train in the use of our Leica SP8-X confocal microscope.
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