NEW: SARS-COV-2 Assay for Neutralizing Antibodies. Integral Molecular has developed a new assay to test for SARS-CoV-2 neutralizing antibodies.
Integral Molecular is a research-driven biotechnology company enabling antibody discovery against complex membrane proteins. The company provides characterization services, reagents, and a full antibody discovery platform optimized to work with membrane protein targets, including GPCRs, ion channels, and transporters.
Founded in 2001, Integral has worked with over 300 different partners and customers, including all top 10 pharmaceutical companies.
Integral Molecular offers pseudotyped Reporter Virus Particles to test infectivity and neutralization for:
• SARS-CoV-2 D614G
• Dengue serotypes 1-4
Reporter Virus Particles (RVPs) are replication-incompetent pseudotyped virus particles that enable safe (BSL-2), easy, and high-throughput viral infectivity and neutralization assays using standard detection instrumentation. RVPs display antigenically correct envelope/spike protein and carry a modified genome that expresses a convenient optical reporter gene (GFP or luciferase) within 24 hours of cellular infection.
RVPs are available as a ready-to-use reagent that provides a safe and efficient alternative to plaque assays, and are produced under quality-controlled conditions as a critical reagent to enable regulatory submissions.
Applications of RVPs
• Antibody neutralization
• Serum screening
• High-throughput assays
Advantages of SARS-CoV-2 RVPs
• Safe in a BSL-2 environment
• Quantitative (luciferase) or fluorescent (GFP) read-out
• Compatible with high-throughput plate-based assays
• Quality-controlled production for use as a critical reagent
Click here for additional information on coronavirus RVPS
Click here for additional information on dengue and Zika RVPs
• Whitbeck ET AL. 2020. Antigenicity, Stability and Reproducibility of Zika Reporter Virus Particles for Long-term Applications. BIORXIV doi.org/10.1101/2020.04.21.047241.
• Mattia ET AL. 2011. Dengue Reporter Virus Particles for Measuring Neutralizing Antibodies Against Each of the Four Dengue Serotypes. PLOS ONE 6(11):e27252. doi: 10.1371/journal.pone.0027252.
25% of antibodies are polyspecific. De-risk your program by choosing the right lead molecule.
Off-target antibody binding is common and can cause serious adverse events
Off-target drug binding can cause serious side effects or even death. We developed the Membrane Proteome Array as the largest array of membrane proteins yet assembled for deorphaning and specificity profiling of biotherapeutics including antibodies and CAR T-cell therapy.
Why is the Membrane Proteome Array so successful?
What can the Membrane Proteome Array do for you?
Specificity Profiling: We screen your antibody to determine its membrane proteome binding profile, identifying target and off-target interactions
Antibody Deorphaning: We screen your antibody of unknown specificity to identify which protein(s) on the MPA your antibody binds
Membrane Proteome Array Case Studies
We Map Conformational Epitopes at Single Amino
Acid Resolution with > 95% Success
Integral Molecular’s proprietary Shotgun Mutagenesis technology is the only high-throughput technique that can reliably map conformational epitopes at single amino acid resolution. We have mapped 1,000+ epitopes to date with > 95% success, including:
High-resolution epitope mapping enables
How Does Shotgun Mutagenesis Work?
We mutate each residue of a target protein and measure the precise binding of an antibody to the target within intact cells.
Epitope Mapping Projects Include
Shotgun Mutagenesis Epitope Mapping Case Studies
Select Publications Featuring Shotgun Mutagenesis Epitope Mapping
The MPS Antibody Discovery platform overcomes the key challenges in isolating antibodies against difficult targets by:
Lipoparticles are virus-like particles that concentrate and present conformationally intact membrane proteins on non-infectious particles. This enables complex membrane proteins to be manipulated as soluble, high-concentration proteins for antibody immunization and screening.
Why are Lipoparticles so effective for antibody discovery?
Lipoparticle applications include:
Antibody screening by ELISA
Kinetic analysis by biosensor
Radioligand and fluorescent binding assays
Lipoparticles are typically customized to incorporate customers’ specified membrane proteins. Each Lipoparticle batch is assessed by using up to 16 quality-control metrics to ensure homogeneity, purity, and target protein integrity. Hundreds of membrane proteins have been successfully incorporated into Lipoparticles, which can be modified with biotin or fluorescence for detection.
Pre-validated Lipoparticles contain optimized, highly expressed membrane proteins and are available for rapid delivery. ReadyReceptor Lipoparticles are produced and validated by using the same stringent quality metrics as are used for our Custom Lipoparticle production. See all ReadyReceptor Lipoparticles.
Select publications that feature Lipoparticles
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