Intact Genomics, Inc.

Intact Genomics offers the highest quality competent cells at very competitive prices. Our DH5-Alpha competent cells (ig™ 5-alpha), 10-Beta competent cells (ig™ 10B), and BL21 (DE3) competent cells have achieved the highest efficiencies in the market. We also provide custom competent cells to meet your specific needs.

-Large-insert DNA cloning and manipulation
-Clone large DNA fragment(s) into BAC, Fosmid, or custom vector(s)

Through innovative production methods and protocols developed by scientists with a combined 90 years of laboratory experience we produce the best results at affordable prices.

• Whole Genome Sequencing
• de novo Sequencing
• Exome Sequencing
• RNA Sequencing
• Small RNA Sequencing
• Targeted Re-Sequencing
• Metagenome Sequencing (16S rRNA Sequencing)

Intact Genomics offers high-quality next generation sequencing services on the Illumina MiSeq platform to support a broad range of genomic applications. Our system is capable of producing either single or paired-end reads, up to 15 Gb of output with 25M sequencing reads and 2x 300 bp read lengths. Our highly trained NGS Specialists, along with this innovative system enable a broad range of applications from targeted gene sequencing to metagenomics, small genome sequencing, targeted gene expression analysis, and more.

BAC Library Service (Random Shear, Conventional or Fosmid Library)

Unbiased BAC libraries without gaps, complete coverage, dramatically reduced finishing costs.

Because of the very large insert sizes, constructing BAC libraries are far more challenging than making typical, small-insert plasmid libraries. Even if successful, conventional BAC libraries are inherently biased and incomplete. Intact Genomics has developed novel random shear technologies that overcome many of these problems, producing more even, complete, and higher quality genomic libraries.

Using our unique random shear BAC colony technology, researchers can get even more accurate, better results.

Conventional BAC Library Service and Fosmid Library Service also available.

Custom chemically competent or electrocompetent E. coli cells.
Highest transformation efficiencies currently available in the industry; >1 x 10^10 cfu/µg DNA chemical competent cells.

Any E. coli strain may be made to the highest possible transformation efficiency, starting from or your own cells or ours.

From plant, animal, bacteria, fungi and human samples. We can prepare high-quality, HMW genomic DNA from plant, animal, Human, bacteria, fungi including soil and other complex sources. This DNA can be used for library construction, PCR, BAC end sequencing, and next-gen sequencing.

Using proprietary methods, we prepare ultra pure, high concentration DNA from individual BAC clones, 96-well or 384-well plates for gene discovery, gene functional study and DNA sequencing.
BAC clones from individual 384-well BAC library plates are inoculated into four 96-deep well plates, cultured, and processed using proprietary methods. The purified DNA is provided in four 96-well Miniprep DNA plates.

• Colony picking, re-arraying, and duplicating: Robotic colony picking, re-arraying and duplicating with high quality control, zero empty wells
  
• DNA Pooling/SuperPooling: Use PCR to screen pools of clones to identify clone(s) of interest, no radioactive materials needed, speed discovery and reduce screening costs
  
• BAC/Fosmid end sequencing: Up to 700 bp per run, minimum of 90% successful reads

Intact Genomics offers a variety of high quality enzymes at the lowest prices. Enzymes Include (but not limited too):

T4 DNA ligase
T4 DNA Polymerase
T4 Polynucleotide Kinase (PNK)
Taq DNA Polymerase
Cas9 Nuclease

Please refer to the resource section for further details regarding all of our products and pricing