Infectious Disease Resource Core

Dr. Wolk's Infectious Disease Research Core operates a research-ready biorepository called the GermBank©, which is compliant with Good Laboratory Practice (GLP) regulations, and human subject's law. Specimens are de-coded for privacy and warehoused with the latest storage technology, such as the GenVault system for room temperature storage of nucleic acids, as well as traditional -80°C and -20 °C storage for clinical specimens. Software is used to track and retrieve isolates and to query the database; software chronologically controls and records the sample management process.
The following focused banks are maintained within the GermBank:
MicroBANK for Microbes: The MicroBANK is a historical repository derived from "excess diagnostic specimens" and antimicrobial-resistant bacterial cultures, which were submitted for diagnostic purposes or infection control investigations.
ImmunoBANK for Human Immune Response Specimens: The ImmunoBANK is a historical repository of whole blood, serum, and plasma derived from "excess diagnostic specimens" which were submitted for diagnostic purposes. The specimens are archived for assessment of immune proteins and cytokines.
GenoBank for Human Genetic Predisposition: The GenoBank is a historical repository of whole blood and human cells, derived from "excess diagnostic specimens" which were submitted for diagnostic purposes. The specimens are archived for assessment of single nucleotide polymorphisms (SNPS) and other mutations.

AdvanDx: GNR Traffic Light PNA FISH Assay
BioRad, Inc: MRSASelect
Cepheid, Inc: GeneXpert® SA/MRSA Assay (wounds); GeneXpert® SA/MRSA Assay (blood cultures); GeneXpert® VanA/B Assay; GeneXpert® MRSA Assay (nares)
Microphage, Inc: MRSA Assay

-Microbial and Human Biorepository Services: MicroBank, ImmunoBank, CellBank, DNA Bank
-Consulting
-Education

PCR/ESI Mass Spectrometry, T5000

Molecular Method Verification and Validation

Nucleic Acid Extraction, Qiagen Biorobot EZ-1
Nucleic Acid Quantitation and Characterization, Agilent 2100
Nucleic Acid Quantitation, NanoDrop

Liquid Microarray, Luminex 200

Bacterial and Fungal Phenotype, Vitek 2 Compact
Blood Culture, BacT/ALERT
Clinical Microbiology Cultures and Antimicrobial Testing Services per CLSI

Nucleic Acid Extraction, Qiagen Biorobot EZ-1
Nucleic Acid Quantitation and Characterization, Agilent 2100
Nucleic Acid Quantitation, NanoDrop

Nucleic Acid Extraction, Qiagen Biorobot EZ-1
Nucleic Acid Quantitation and Characterization, Agilent 2100
Nucleic Acid Quantitation, NanoDrop

Molecular Epidemiology, Bacterial Genotype by Diversilab rep-PCR

Real-time PCR and QPCR, Mastercycler epRealPlex