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ImmunoPrecise Antibodies Ltd

11 Orders Completed
Victoria, British Columbia, CA

About ImmunoPrecise Antibodies Ltd

ImmunoPrecise and ModiQuest have Proprietary Technologies Developed for Your Success:

ImmunoPrecise Antibodies (IPA) operates globally to accelerate therapeutic antibody discovery by offering a full suite of innovative technologies yielding superior candidate diversity and... Show more »

ImmunoPrecise and ModiQuest have Proprietary Technologies Developed for Your Success:

ImmunoPrecise Antibodies (IPA) operates globally to accelerate therapeutic antibody discovery by offering a full suite of innovative technologies yielding superior candidate diversity and out-performing traditional methods.

Immunization:

  • DNA, protein, peptide, whole cell immunization
  • Proprietary immunization methods, including rapid protocols
  • Species include mouse, rat, camelid, rabbit, chicken, goat, more
  • Proven success with multiple transgenic species/strains

Antibody Discovery:

  • Expedited discovery through our superior B cell selection technology
  • Advanced custom and in-house phage library screening
  • Human and naive llama libraries (VHH and scFv) available
  • Rodent and rabbit monoclonal development
  • State-of-the-art hybridoma generation, including semi-solid media and clone picking

Completing the Full Value Chain:

  • Characterization
  • Engineering and humanization
  • Optimization
  • Bi-specifics development and manufacturing
  • Recombinant protein manufacturing
  • Pre-clinical studies

 

ModiQuest focuses on offering high-quality custom antibody generation and development solutions fully tailored to the needs and interests of its clients and partners.  Since its creation in 2004, ModiQuest successfully performed over 250 antibody projects for clients in Europe, the US, Asia and Russia.

 

Over the past 10 years, ModiQuest has built a unique state-of-the-art and fully integrated custom antibody generation and development platform, including antigen design, antibody lead generation, optimization and production.  Depending on our clients’ in-house capabilities and the priority and difficulty of their target of interest, they can access the full ModiQuest platform (“from antigen to the production of an optimized antibody”) or choose specific parts of our platform.

 

To date, ModiQuest has successfully completed over 250 antibody generation and development projects for clients in Europe, the US, Asia and Russia. Typically, we run over 25 different client projects per year with a maximum of 10 projects at any given time.

 

The ModiQuest team consists of highly skilled and experienced scientists and professionals, fully dedicated and focused on the successful execution and completion of client projects. In addition, the team is constantly working on optimizing and expanding our technology platform to ensure unique and high-quality services and product offering.

 

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Diversity Certificates

Small business

Our Services (78)


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Therapeutic Monoclonal Antibody (mAb) Development

Price on request

IPA is a single source provider of services for the complete antibody discovery value chain. We offer the most comprehensive collection of antibody discovery platforms in the industry, combined with state of the art lead candidate optimization and characterization. This allows us to assist clients from the moment they identify a... Show more »

IPA is a single source provider of services for the complete antibody discovery value chain. We offer the most comprehensive collection of antibody discovery platforms in the industry, combined with state of the art lead candidate optimization and characterization. This allows us to assist clients from the moment they identify a therapeutic target to the preparation of applying for investigational review of the clinical product.

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iQue Rabbit Chicken Mouse Rat Humanized Mouse Humanized Rat Camelids Immuno-Oncology Recombinant hybridoma phage display ultra-humanization focus library design and screening B cell select GPCR Show 16 more tags Show less

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Phage Display Library Construction and Screening

Price on request

An antibody generation approach based on the selection of antigen specific recombinant antibody fragments from proprietary human or llama phage libraries or from custom build immune libraries. Our proprietary libraries have been made from human patient and naïve repertoires, as well as from naïve llama (VHH and scFv) repertoire.... Show more »

An antibody generation approach based on the selection of antigen specific recombinant antibody fragments from proprietary human or llama phage libraries or from custom build immune libraries. Our proprietary libraries have been made from human patient and naïve repertoires, as well as from naïve llama (VHH and scFv) repertoire. Custom immune libraries are prepared from spleen, lymph nodes, and bone marrow of immunized animals or humans. This approach captures the immune repertoire of immunized animals or patients and allows sampling of this repertoire by semi-automatic panning procedures, to rescue and identify unique antibodies with pre-specified characteristics. Antibody fragments can be expressed in bacteria or rapidly reformatted into any given Ig format, such as full size IgG of various isotypes, Fc mutants to modify effector functions, or chimera with Fc regions from different species.

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Human Rabbit Chicken Humanized Mouse Humanized Rat Camelids Show 6 more tags Show less

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Monoclonal Antibody (mAb) Production

Price on request

We can custom develop mAbs to an array of antigens including peptides, modified peptides, recombinant proteins, DNA plasmids, small molecules and anti-idiotypic antibodies.

Rabbit Monoclonal Antibody Development

ImmunoPrecise is excited to offer custom rabbit recombinant monoclonal antibody development through our... Show more »

We can custom develop mAbs to an array of antigens including peptides, modified peptides, recombinant proteins, DNA plasmids, small molecules and anti-idiotypic antibodies.

Rabbit Monoclonal Antibody Development

ImmunoPrecise is excited to offer custom rabbit recombinant monoclonal antibody development through our proprietary B cell Select platform.

Mouse Rapid Prime Monoclonal Antibody Development

Rapid Prime is ImmunoPrecise’s proprietary custom monoclonal antibody development service. This rapid immunization strategy saves weeks in development time. When coupled with our Single-Step cloning procedure, positive hybridoma cell lines are identified in 35 days. This rapid immunization procedure does not compromise on affinity. It consistently generates large numbers of IgG clones specific for antigen.

This popular choice for antibody development can be paired with 2 standard method mice which serve as a contingency plan. Contact us and ask about our “Rapid Prime plus Contingency” antibody design.

Standard Method Monoclonal Antibody Development in Mice & Rats

ImmunoPrecise’s custom standard monoclonal antibody development service is the gold standard in antibody development. Intraperitoneal injections and a choice of adjuvant is available to suit the clients specific needs. When this traditional immunization procedure is coupled with our Single-Step cloning method, weeks are shaved off the timeline of monoclonal development.

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Rabbit Mouse Rat

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Antibody Production by DNA Immunization

Price on request

A novel immunization platform for the generation of monoclonal antibodies against challenging and difficult to express conformation-sensitive targets, including complex protein classes such as ion channels and GPCRs. Within a DNA immunization approach, the antigens will be expressed in vivo in the animal and thereby go through the... Show more »

A novel immunization platform for the generation of monoclonal antibodies against challenging and difficult to express conformation-sensitive targets, including complex protein classes such as ion channels and GPCRs. Within a DNA immunization approach, the antigens will be expressed in vivo in the animal and thereby go through the post-transcriptional modification process and therefore the antigens maintain their naïve conformation. DNA immunization is much more efficient when compared with traditional protein-based immunizations, since it evades the time-consuming requirement to express and purify the immunogens.

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GPCR Ion Channel

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Antibody Discovery

Price on request

B cell Antibody Discovery

This proprietary technology allows for selection of target specific B-cells from any tissue in a variety of animal species.

B cell Antibody Discovery

This proprietary technology allows for selection of target specific B-cells from any tissue in a variety of animal species.

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Rabbit Chicken Mouse Rat Immuno-Oncology GPCR Show 6 more tags Show less

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Bispecific Antibody Production

Price on request

Access to our royalty-free human scFv and llama VHH repertoires, along with expertise in protein and antibody engineering, positions ImmunoPrecise as a desirable provider for bispecific antibody development.

Access to our royalty-free human scFv and llama VHH repertoires, along with expertise in protein and antibody engineering, positions ImmunoPrecise as a desirable provider for bispecific antibody development.

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In Silico Protein Sequence Analysis

Price on request
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In silico Antibody Humanization

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Antibody Development

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Phospho-Specific Antibody Production

Price on request

ImmunoPrecise successfully develops high-affinity, phosphospecific mAbs with our rabbit B Cell Select platform, through both our rapid 28-day immunization strategy and standard 78-day protocol.

ImmunoPrecise successfully develops high-affinity, phosphospecific mAbs with our rabbit B Cell Select platform, through both our rapid 28-day immunization strategy and standard 78-day protocol.

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antibody discovery

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Antibody Engineering

Price on request
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Hybridoma Development

Price on request

Innovative immunization protocols, including rapid methods, and advanced hybridoma generation including semi-solid media and clone picking.

Innovative immunization protocols, including rapid methods, and advanced hybridoma generation including semi-solid media and clone picking.

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Mouse Rat

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Animal Immunization for Antibody Production

Price on request

Innovative immunization methods, including rapid protocols, and many alternative species and strains available.

Innovative immunization methods, including rapid protocols, and many alternative species and strains available.

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Peptide Synthesis

Price on request

ImmunoPrecise offers both peptide synthesis and protein carrier conjugation services. We recommend KLH (keyhole limpet hemocyanin) for immunizing antigen conjugation and BSA (bovine serum albumin) for screening peptide conjugation. Other carrier proteins are available upon request. Please note: all peptides require a terminal... Show more »

ImmunoPrecise offers both peptide synthesis and protein carrier conjugation services. We recommend KLH (keyhole limpet hemocyanin) for immunizing antigen conjugation and BSA (bovine serum albumin) for screening peptide conjugation. Other carrier proteins are available upon request. Please note: all peptides require a terminal Cysteine residue for conjugation purposes.

ImmunoPrecise offers a variety of specialized and modified amino acids as well as an array of different coupling and branching options in a range of different scales and purities. IPA will work with you to assist you in epitope prediction and design of your antigen.

We strive to provide you with the most reliable and high-quality peptides to get your antibody development projects off in the right direction.

Peptides are used as antigens for a variety of reasons (epitope targeting, avoiding cross-reactivity, difficult antigen to express) and proper epitope prediction will give you the best chance to achieve your antibody goals. Contact us about multiplexing your peptide project.

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Antibody Characterization

Price on request

Antibody leads are screened and characterized to determine optimal purity, reactivity, aggregation, functionality, and affinity.

Antibody leads are screened and characterized to determine optimal purity, reactivity, aggregation, functionality, and affinity.

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Antibody Affinity Maturation

Price on request

Antibody affinity is important in therapeutic and diagnostic applications. ModiQuest’s antibody affinity maturation service can improve antibodies affinities by several fold. We can apply different strategies to increase the affinity of your antibody, including gene shuffling and random mutagenesis.

Antibody affinity is important in therapeutic and diagnostic applications. ModiQuest’s antibody affinity maturation service can improve antibodies affinities by several fold. We can apply different strategies to increase the affinity of your antibody, including gene shuffling and random mutagenesis.

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Antibody Humanization

Price on request

Animal-derived monoclonal antibodies can elicit human anti-animal antibody responses in patients. Therefore it is usually necessary to replace the animal content with the corresponding human amino acid residues. We provide a robust and efficient antibody humanization service, which consistently retains affinity and specificity... Show more »

Animal-derived monoclonal antibodies can elicit human anti-animal antibody responses in patients. Therefore it is usually necessary to replace the animal content with the corresponding human amino acid residues. We provide a robust and efficient antibody humanization service, which consistently retains affinity and specificity levels. Our approach is based on state of the art in silico antibody modeling to identify essential framework and CDR residues for grafting onto a human antibody framework.

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Antibody Sequencing

Price on request

B-Cell Select:

Heavy and light chain sequences for recombinant DNA constructs of all client-chosen top clones are included as part of B-cell project deliverables.

Hybridoma sequencing:

-Isotype/subisotype determination
-Mycoplasma Testing
-RNA Isolation, cDNA preparation, PCR amplification
-DNA sequencing of... Show more »

B-Cell Select:

Heavy and light chain sequences for recombinant DNA constructs of all client-chosen top clones are included as part of B-cell project deliverables.

Hybridoma sequencing:

-Isotype/subisotype determination
-Mycoplasma Testing
-RNA Isolation, cDNA preparation, PCR amplification
-DNA sequencing of antibody heavy and light chain variable regions
-Cloning of heavy and light chain variable regions into mammalian expression system
-Transient test expression of antibody in mammalian cell line to determine expression levels
-Confirmation of antigen binding by indirect ELISA

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B cells

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Protein Services

Price on request

We are able to recombinantly express small to large (>10g) scale levels of protein and antibody. Dedicated purification protocols yield highly pure protein preparations with extreme low endotoxin levels, in general below 0.05 EU/mg. We can also carry out stable cell line generation.

We are able to recombinantly express small to large (>10g) scale levels of protein and antibody. Dedicated purification protocols yield highly pure protein preparations with extreme low endotoxin levels, in general below 0.05 EU/mg. We can also carry out stable cell line generation.

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Recombinant mammalian cell expression

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Polyclonal Antibody Production

Price on request

ImmunoPrecise is a leading producer of high-titre custom rabbit polyclonal antibodies. We offer a comprehensive range of services to get you the polyclonal product you need, including epitope prediction, custom peptide synthesis, conjugation, immunization, purification and assay design.

If you would like to start a rabbit... Show more »

ImmunoPrecise is a leading producer of high-titre custom rabbit polyclonal antibodies. We offer a comprehensive range of services to get you the polyclonal product you need, including epitope prediction, custom peptide synthesis, conjugation, immunization, purification and assay design.

If you would like to start a rabbit polyclonal with the option to make a monoclonal antibody please check out our RMAT service page.

IPA also offers polyclonal production in mice, rats, goats and sheep. Please contact us for more information.

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Rabbit Sheep Goat Mouse Rat

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Antibody Purification

Price on request

We are able to purify antibodies from a variety of sources including ascites, sera and tissue culture supernatant (bioreactor concentrated and other).

We are able to purify antibodies from a variety of sources including ascites, sera and tissue culture supernatant (bioreactor concentrated and other).

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Cryopreservation

Price on request

In the event of an emergency, it is crucial to have a secondary stock of your valuable biological materials stored at an alternative location. ImmunoPrecise provides you with a secure, off-site storage facility to safeguard your assets. We have the capability to store cell lines, tissues, RNA, DNA, plasmid constructs, antibodies,... Show more »

In the event of an emergency, it is crucial to have a secondary stock of your valuable biological materials stored at an alternative location. ImmunoPrecise provides you with a secure, off-site storage facility to safeguard your assets. We have the capability to store cell lines, tissues, RNA, DNA, plasmid constructs, antibodies, and many other biological materials.

Storage Options

  • +4°C
  • -20°C
  • -80°C
  • -196°C (Liquid Nitrogen, LN­2)

Features

  • We offer storage by the box, rack, shelf or freezer. Please ask about bulk pricing.
  • Experienced international or domestic shipping and receiving.
  • No minimum storable quantity.
  • Customized software inventory system.
  • Equipment is monitored on a daily basis by qualified personnel.
  • Master and Working stock preparation and other cell line banking options available. Please enquire.

Equipment

  • A dedicated uninterruptible power supply and diesel generator supplies more than 100% of freezer power needs.
  • All liquid nitrogen storage is done in late model high efficiency containers.
  • All material to be stored at -80°C is placed in late model ULT freezers.
  • All equipment maintenance is documented and equipment is tested and certified annually.

Security

  • Additional LN2 is stored in bulk to ensure an uninterrupted supply.
  • On site security providing 24 hours 7 days a week monitoring and alarm response.
  • Building and facility access is controlled by electronic key card.
  • All storage units are secured, earthquake protected and pad locked to strictly control all access to samples.

Should you require a quote IPA’s cryostorage services, please reach us at 1-250-483-0308, via email, or try our online quote request form.

Freezing/Cryopreservation of Hybridomas (and other cell lines) Standard Operating Procedure

Most hybridomas are frozen in either growth media with 20% fetal bovine serum (FBS) and 10% dimethylsulfoxide (DMSO) or 90% FBS plus 10% DMSO and stored in liquid nitrogen. IPA freezes hybridomas in 90% FBS plus 10% DMSO in 1mL (2 x 10e6 cells/vial) aliquots. Always wash DMSO away when thawing. Thaw frozen vials directly from liquid nitrogen in a 37°C water bath for exactly 2 minutes. Immediately transfer cells to 10mL DMEM complete media plus 10% FBS in 15mL conical tube, mix gently by inverting several times, spin down at 200 x g for 6 minutes. Transfer pellet to 10mL DMEM complete media plus 20% FBS in pre-gassed 25cm2 flask. FBS content is reduced to 10% once cells are recovered (2-3 days post thaw).

Freeze in 1mL aliquots of 90% FBS and 10% DMSO at 2 x 10e6 cells per vial. Split cells ½ for at least 2 days before freezing while maintaining them at mid log. Freshly divided cells have stronger membranes that withstand the freezing process better. Keep freezing media ice cold during the freezing process as DMSO is toxic to cells at warmer temperatures.

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Cells and Tissues

Price on request

ImmunoPrecise’s B cell platform uses innovative technology that allows for antibody screening from any species of animals, from any tissue sample, allowing for a greater diversity of animal repertoires and for faster, deeper antibody screening compared to traditional technologies.

ImmunoPrecise’s B cell platform uses innovative technology that allows for antibody screening from any species of animals, from any tissue sample, allowing for a greater diversity of animal repertoires and for faster, deeper antibody screening compared to traditional technologies.

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Cryostorage and Cryoprocessing

Price on request

Storage Options:
+4°C
-20°C
-80°C
-196°C (Liquid Nitrogen, LN­2)

Storage Options:
+4°C
-20°C
-80°C
-196°C (Liquid Nitrogen, LN­2)

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Antibody Isotyping

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Protein Purification

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Hybridoma Screening

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Hybridoma Fusion

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Cell Lines

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Antibody Fragment Production

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Animal Models and Studies

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Antigen Expression and Purification

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Cell Line Generation

Price on request

Our stable cell line generation service offeres the generation of stable high-level antibody producing cell lines via our optimized expression platform, ModiXpress™, ClonePix FL technology and/or MTX amplification.

Our stable cell line generation service offeres the generation of stable high-level antibody producing cell lines via our optimized expression platform, ModiXpress™, ClonePix FL technology and/or MTX amplification.

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Biomolecular Interaction Analysis

Price on request

Biomolecular Interaction Analysis Services

Biomolecular Interaction Analysis Services

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Custom Cell-Based Assay Development

Price on request

ModiQuest Research offers custom assay development services fully tailored to the needs of our clients. In addition, we have developed a number of assays in-house, which we also offer on a fee-for-service basis (e.g. anti-TNF bioactivity assays, CAIA mouse model, etc.).

ModiQuest Research offers custom assay development services fully tailored to the needs of our clients. In addition, we have developed a number of assays in-house, which we also offer on a fee-for-service basis (e.g. anti-TNF bioactivity assays, CAIA mouse model, etc.).

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Hybridoma Sequencing

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Antibody Reformatting

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Our antibody sequencing, cloning, reformatting, and expression service can clone and express any variable antibody domain into any immunoglobulin context required, such as full size mouse, rabbit, chicken, human format, chimeras with any Fc region of choice, including effector mutants or Fc fusions.

Our antibody sequencing, cloning, reformatting, and expression service can clone and express any variable antibody domain into any immunoglobulin context required, such as full size mouse, rabbit, chicken, human format, chimeras with any Fc region of choice, including effector mutants or Fc fusions.

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Antibody Screening

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Recombinant Antibody Production

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Custom Monoclonal Antibody (mAb) Production

Price on request

For over 10 years ModiQuest Research has specialized in the generation of monoclonal antibodies against difficult target antigens for R&D, diagnostic and therapeutic applications. Using our proprietary ModiVacc (cell immunisation) ModiFuse (electrofusion hybridoma), ModiSelect (B-cell selection) and ModiPhage (phage display)... Show more »

For over 10 years ModiQuest Research has specialized in the generation of monoclonal antibodies against difficult target antigens for R&D, diagnostic and therapeutic applications. Using our proprietary ModiVacc (cell immunisation) ModiFuse (electrofusion hybridoma), ModiSelect (B-cell selection) and ModiPhage (phage display) technologies, we can generate monoclonal antibodies from various backgrounds such as mouse, rat, rabbit, chicken, llama and human against virtually any target.

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Antigen Design

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Transient Antibody Production

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Up to gram scale (~300mg/L) transient production of antibodies using our in-house optimized expression platform, ModiXpress™.

Up to gram scale (~300mg/L) transient production of antibodies using our in-house optimized expression platform, ModiXpress™.

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Membrane Protein Antibody Production

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Cell Line Rescue

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Hybridoma Production

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Biochemistry & Molecular Biology

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Mammalian Cell Culture

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Cell and Tissue Culture

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ScFv Expression

Single Chain Variable Fragment Expression
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In vitro Monoclonal Antibody (mAb) Production

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Monoclonal Antibody (mAb) Production from Plasmids

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Monoclonal Antibody (mAb) Production from Synthetic Peptides

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DNA Services

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Project Management

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Cell-Based Assays

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Mutagenesis

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Antibody Products

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Purified Proteins

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Purified Proteins Services

Purified Proteins Services

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Fusion Proteins

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Nanobody Libraries

Single Domain Antibody Library
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Cytometry

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Cytometry Services

Cytometry Services

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Flow Cytometry Experimental Design

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Hybridoma Subcloning

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Anti-Idiotype Antibody Production

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Recombinant Antigen Development

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Transfection

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Live Cell Assay Development

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Protein Production

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Biology

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Bioconjugation/Chemical Crosslinking

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Random Mutagenesis

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Assay Development

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Flow Cytometry

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Fluorescence Activated Cell Sorting (FACS)

Fluorescence Activated Cell Sorting
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Antibody Modification

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Antibody Services

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Specialized Cell-Based Assays

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Nucleic Acid Services

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