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ImmunoPrecise Antibodies

Victoria, British Columbia, CA

ImmunoPrecise Antibodies Ltd. (IPA) is a privately owned Canadian company located in Victoria, British Columbia, Canada. Our dedication to advancing antibody development has resulted in cutting-edge immunological innovations. With over 25 years of experience in the custom antibody business, IPA provides high customer satisfaction through a customized professional approach.

IPA is comprised of experienced research and development scientists and encompasses a wide range of distinctive competencies, including innovative technologies required in the production of monoclonal and polyclonal antibodies. We pride ourselves on our high customer satisfaction and our clients have grown accustomed to receiving very thorough and professional service. We customize your project to generate antibodies that meet your specific needs. Our clients include: scientists in the biotech and pharmaceutical sector, academic researchers and... Show more »

ImmunoPrecise Antibodies Ltd. (IPA) is a privately owned Canadian company located in Victoria, British Columbia, Canada. Our dedication to advancing antibody development has resulted in cutting-edge immunological innovations. With over 25 years of experience in the custom antibody business, IPA provides high customer satisfaction through a customized professional approach.

IPA is comprised of experienced research and development scientists and encompasses a wide range of distinctive competencies, including innovative technologies required in the production of monoclonal and polyclonal antibodies. We pride ourselves on our high customer satisfaction and our clients have grown accustomed to receiving very thorough and professional service. We customize your project to generate antibodies that meet your specific needs. Our clients include: scientists in the biotech and pharmaceutical sector, academic researchers and those employed by government.

Since its inception in 1989, IPA has been serving clinical research and biological markets worldwide. With its beginnings in the Biochemistry/Microbiology department at the University of Victoria, IPA expanded and built a new Hybridoma Facility, at the Vancouver Island Technology Park (VITP) in 2005.

Robert Beecroft, CEO and Founder, has implemented 3 key methodologies that have allowed IPA to be set apart from other antibody companies: 1) the Rapid Prime immunization strategy in mice; 2) Single-Step cloning for HAT selection of hybridomas and 3) RMAT –recombinant monoclonal antibody technology for the generation of rabbit monoclonal antibodies.

IPA uses proprietary and innovative inoculation and cloning technologies and established expertise to produce mAbs significantly faster than traditional methods of antibody production and has used these capacities to produce hundreds of mAbs, many of which are now being sold commercially through industry partners.

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Animal Immunization for Antibody Production
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Rabbit
Sheep
Goat
Mouse
Rat
Peptide Synthesis
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ImmunoPrecise offers both peptide synthesis and protein carrier conjugation services. We recommend KLH (keyhole limpet hemocyanin) for immunizing antigen conjugation and BSA (bovine serum albumin) for screening peptide conjugation. Other carrier proteins are available upon request. Please note: all peptides require a terminal... Show more »

ImmunoPrecise offers both peptide synthesis and protein carrier conjugation services. We recommend KLH (keyhole limpet hemocyanin) for immunizing antigen conjugation and BSA (bovine serum albumin) for screening peptide conjugation. Other carrier proteins are available upon request. Please note: all peptides require a terminal Cysteine residue for conjugation purposes.

ImmunoPrecise offers a variety of specialized and modified amino acids as well as an array of different coupling and branching options in a range of different scales and purities. IPA will work with you to assist you in epitope prediction and design of your antigen.

We strive to provide you with the most reliable and high-quality peptides to get your antibody development projects off in the right direction.

Peptides are used as antigens for a variety of reasons (epitope targeting, avoiding cross-reactivity, difficult antigen to express) and proper epitope prediction will give you the best chance to achieve your antibody goals. Contact us about multiplexing your peptide project.

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Antibody Purification
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We are able to purify antibodies from a variety of sources including ascites, sera and tissue culture supernatant (bioreactor concentrated and other).

We are able to purify antibodies from a variety of sources including ascites, sera and tissue culture supernatant (bioreactor concentrated and other).

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Cryopreservation
Price on request

In the event of an emergency, it is crucial to have a secondary stock of your valuable biological materials stored at an alternative location. ImmunoPrecise provides you with a secure, off-site storage facility to safeguard your assets. We have the capability to store cell lines, tissues, RNA, DNA, plasmid constructs, antibodies,... Show more »

In the event of an emergency, it is crucial to have a secondary stock of your valuable biological materials stored at an alternative location. ImmunoPrecise provides you with a secure, off-site storage facility to safeguard your assets. We have the capability to store cell lines, tissues, RNA, DNA, plasmid constructs, antibodies, and many other biological materials.

Storage Options

  • +4°C
  • -20°C
  • -80°C
  • -196°C (Liquid Nitrogen, LN­2)

Features

  • We offer storage by the box, rack, shelf or freezer. Please ask about bulk pricing.
  • Experienced international or domestic shipping and receiving.
  • No minimum storable quantity.
  • Customized software inventory system.
  • Equipment is monitored on a daily basis by qualified personnel.
  • Master and Working stock preparation and other cell line banking options available. Please enquire.

Equipment

  • A dedicated uninterruptible power supply and diesel generator supplies more than 100% of freezer power needs.
  • All liquid nitrogen storage is done in late model high efficiency containers.
  • All material to be stored at -80°C is placed in late model ULT freezers.
  • All equipment maintenance is documented and equipment is tested and certified annually.

Security

  • Additional LN2 is stored in bulk to ensure an uninterrupted supply.
  • On site security providing 24 hours 7 days a week monitoring and alarm response.
  • Building and facility access is controlled by electronic key card.
  • All storage units are secured, earthquake protected and pad locked to strictly control all access to samples.

Should you require a quote IPA’s cryostorage services, please reach us at 1-250-483-0308, via email, or try our online quote request form.

Freezing/Cryopreservation of Hybridomas (and other cell lines) Standard Operating Procedure

Most hybridomas are frozen in either growth media with 20% fetal bovine serum (FBS) and 10% dimethylsulfoxide (DMSO) or 90% FBS plus 10% DMSO and stored in liquid nitrogen. IPA freezes hybridomas in 90% FBS plus 10% DMSO in 1mL (2 x 10e6 cells/vial) aliquots. Always wash DMSO away when thawing. Thaw frozen vials directly from liquid nitrogen in a 37°C water bath for exactly 2 minutes. Immediately transfer cells to 10mL DMEM complete media plus 10% FBS in 15mL conical tube, mix gently by inverting several times, spin down at 200 x g for 6 minutes. Transfer pellet to 10mL DMEM complete media plus 20% FBS in pre-gassed 25cm2 flask. FBS content is reduced to 10% once cells are recovered (2-3 days post thaw).

Freeze in 1mL aliquots of 90% FBS and 10% DMSO at 2 x 10e6 cells per vial. Split cells ½ for at least 2 days before freezing while maintaining them at mid log. Freshly divided cells have stronger membranes that withstand the freezing process better. Keep freezing media ice cold during the freezing process as DMSO is toxic to cells at warmer temperatures.

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Monoclonal Antibody (mAb) Production
Price on request

We can custom develop mAbs to an array of antigens including peptides, modified peptides, recombinant proteins, DNA plasmids, small molecules and anti-idiotypic antibodies.

Rabbit Monoclonal Antibody Development

ImmunoPrecise is excited to offer custom Rabbit Monoclonal Antibody development service using RMAT (Recombinant... Show more »

We can custom develop mAbs to an array of antigens including peptides, modified peptides, recombinant proteins, DNA plasmids, small molecules and anti-idiotypic antibodies.

Rabbit Monoclonal Antibody Development

ImmunoPrecise is excited to offer custom Rabbit Monoclonal Antibody development service using RMAT (Recombinant Monoclonal Antibody Technology).

Mouse Rapid Prime Monoclonal Antibody Development

Rapid Prime is ImmunoPrecise’s proprietary custom monoclonal antibody development service. This rapid immunization strategy saves weeks in development time. When coupled with our Single-Step cloning procedure, positive hybridoma cell lines are identified in 65 days. This rapid immunization procedure does not compromise on affinity. It consistently generates large numbers of IgG clones specific for antigen. Click here for more Rapid Prime information.

This popular choice for antibody development can be paired with 2 standard method mice which serve as a contingency plan. Contact us and ask about our “Rapid Prime plus Contingency” antibody design.

Standard Method Monoclonal Antibody Development in Mice & Rats

ImmunoPrecise’s custom standard monoclonal antibody development service is the gold standard in antibody development. Intraperitoneal injections and a choice of adjuvant is available to suit the clients specific needs. When this traditional immunization procedure is coupled with our Single-Step cloning method, weeks are shaved off the timeline of monoclonal development.

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Rabbit
Mouse
Rat
Antibody Characterization
Price on request

ImmunoPrecise is capable of small-scale as well as bulk production of any antibody clone (mouse and rat) both in vitro (bioreactors) and as in vivo (as ascites) with short turnaround times.

ImmunoPrecise is capable of small-scale as well as bulk production of any antibody clone (mouse and rat) both in vitro (bioreactors) and as in vivo (as ascites) with short turnaround times.

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Polyclonal Antibody Production
Price on request

ImmunoPrecise is a leading producer of high-titre custom rabbit polyclonal antibodies. We offer a comprehensive range of services to get you the polyclonal product you need, including epitope prediction, custom peptide synthesis, conjugation, immunization, purification and assay design.

If you would like to start a rabbit... Show more »

ImmunoPrecise is a leading producer of high-titre custom rabbit polyclonal antibodies. We offer a comprehensive range of services to get you the polyclonal product you need, including epitope prediction, custom peptide synthesis, conjugation, immunization, purification and assay design.

If you would like to start a rabbit polyclonal with the option to make a monoclonal antibody please check out our RMAT service page.

IPA also offers polyclonal production in mice, rats, goats and sheep. Please contact us for more information.

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Rabbit
Sheep
Goat
Mouse
Rat
Chemistry and Materials
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Synthetic Chemistry
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Biochemistry & Molecular Biology
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Therapeutic Monoclonal Antibody (mAb) Development
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Biology
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Biospecimens
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Cryostorage
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Cells and Tissues
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Biospecimen Management and Storage
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Antibody Services
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Protein Services
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