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Immunohistochemistry (IHC) Core

ic 10.0/10 Overall Rating 100% Positive
ic 9 Orders Completed
New York, New York, US

About Immunohistochemistry (IHC) Core

The Immunohistochemistry Core Laboratory, a component of the Experimental Pathology Shared Resource, is currently located on the NYULMC main campus in the Smilow Research Building. For Histopathology Core services, such as H&E staining, please visit our sister lab here:... Show more »

The Immunohistochemistry Core Laboratory, a component of the Experimental Pathology Shared Resource, is currently located on the NYULMC main campus in the Smilow Research Building. For Histopathology Core services, such as H&E staining, please visit our sister lab here: https://www.scienceexchange.com/facilities/histopathology-core

The IHC laboratory provides guidance and support for the design and execution of experiments involving histopathology and chromogenic or immunofluorescence immunohistochemistry of human and animal tissues and cells. In collaboration with other components of the Experimental Pathology Shared Resource, the IHC core provides guidance for routine and advanced histopathologic services for cells and tissue specimens, and guidance and expertise for special and routine histochemical staining.

The laboratory serves as immunohistochemical repository with a catalog of over 1200 antibodies. The majority of these antibodies are available for the collection of preliminary or more comprehensive data. In addition, many of these antibodies have been tested in both human and animal tissues, and can be used on any of the laboratories two exisiting Ventana NEXes, or on either Discovery and or Discovery XT automated immunohistochemical staining instruments.

The laboratory optimizes and validates new (commercial or proprietary) antibodies that have not been tested by immunohistochemistry. The laboratory uses both human and animal tissue micro/macro arrays, and can employ a wide variety of antigen retrieval methods to determine the applicability of an antibody to immunohistochemical labeling of fresh, frozen or paraffin embedded cells and tissues. Results can be imaged for immediate review and fast turn around.

The laboratory also prepares human and anaimal Tissue Microarrays using a Pathology Devices semi-automated TMA instrument.

The laboratory faculty and staff are available to provide hands-on formal training, as well as provide consulting for the design and execution of experiments involving histopathology and immunohistochemistry of human and animal cells and tissues. The staff can also assist investigators with routine and advanced histopathologic services for tissue and cell specimens (including guidance on initial processing steps: grossing/tissue preparation, processing, embedding, paraffin/frozen microtomy) for samples destined for histopathological interpretation and analysis.

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Our Services (29)


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Bright-Field Microscopy

Starting at $50.00 per hour

For documenation or publication purposes

For documenation or publication purposes

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VENTANA Discovery XT VENTANA Discovery Classic

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H&E Staining

Hematoxylin and eosin staining
Starting at $4.00 per slide
Request a quote for more information about this service.
VENTANA Discovery XT VENTANA Discovery Classic

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Immunohistochemistry (IHC)

Starting at $22.50 per slide

Base prices are for External Academic Institutions. Pricing is subject to change

Chromogenic:
- Antibody Verification - Verifying antibody staining conditions for in-house antibodies that have not been used for a period of 1 month or more (sectioning service fee not included) $10.50/slide

Base prices are for External Academic Institutions. Pricing is subject to change

Chromogenic:
- Antibody Verification - Verifying antibody staining conditions for in-house antibodies that have not been used for a period of 1 month or more (sectioning service fee not included) $10.50/slide

  • Manual method - Non-automated, standard 2 step indirect (limited to 20 slides at a time per staining run) $45.00/slide

  • Antibody Optimization - Optimizing new or untested antibodies (use of in house TMA sectioning service fee not included) $30.00/slide

  • Double Label - 2 Color sequential double stains (requires prior optimization of both markers) $37.50/slide

  • Routine Automated - For markers with established conditions $22.50/slide

Fluorescent:
- Antibody Optimization - Single color with counter $18.75/slide

  • Antibody Routine- Single color with counter $13.88/slide
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Agilent DAKO VENTANA Discovery XT VENTANA NEXes VENTANA Discovery Classic

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Immunostaining Experimental Design

Starting at $100.00 per hour
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VENTANA Discovery XT VENTANA Discovery Classic

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Immunostaining Training

Starting at $150.00 per hour

Requires pre-approval. Includes both theory and practice. Practical laboratory time dependent on availability and volume.

Requires pre-approval. Includes both theory and practice. Practical laboratory time dependent on availability and volume.

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VENTANA Discovery XT VENTANA Discovery Classic

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Paraffin Embedding

Starting at $9.75 per sample

For cultured cells, utilizing the Plasma-Thromobin or agarose technique. Includes tissue processing, embedding and H&E for QC/QA

For cultured cells, utilizing the Plasma-Thromobin or agarose technique. Includes tissue processing, embedding and H&E for QC/QA

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VENTANA Discovery XT VENTANA Discovery Classic

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Paraffin Sectioning

Starting at $4.50 per slide

Base prices are for External Academic Institutions. Pricing is subject to change

Microtomy, Routine $4.50/slide
- Paraffin sectioning +/- controls for verification, optimization or routine IHC

Microtomy, TMA $12.00/slide
-includes in house control TMA

Base prices are for External Academic Institutions. Pricing is subject to change

Microtomy, Routine $4.50/slide
- Paraffin sectioning +/- controls for verification, optimization or routine IHC

Microtomy, TMA $12.00/slide
-includes in house control TMA

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VENTANA Discovery XT VENTANA Discovery Classic

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Tissue Microarrays

Starting at $225.00 per sample

Base prices are for External Academic Institutions. Pricing is subject to change

Construction - Creating TMA charges are per core inserted $6.00/sample

Set-up - Creating block layout, key and instrument set-up (per/block) $225.00/sample

Base prices are for External Academic Institutions. Pricing is subject to change

Construction - Creating TMA charges are per core inserted $6.00/sample

Set-up - Creating block layout, key and instrument set-up (per/block) $225.00/sample

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VENTANA Discovery XT VENTANA Discovery Classic

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Transmitted Light Microscopy

Price on request
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Tissue Sectioning

Price on request
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Biospecimens

Price on request
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Clinical Laboratory Services

Price on request
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Clinical Research

Price on request
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Cells and Tissues

Price on request
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Tissue Processing

Price on request
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Biology

Price on request
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Histological Staining

Price on request
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Biochemistry & Molecular Biology

Price on request
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Optical Microscopy

Price on request
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Protein Expression Visualization

Price on request
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Common Histochemical Stains

Price on request
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Immunostaining

Price on request
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Protein Services

Price on request
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Assay Development

Price on request
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Tissue Embedding

Price on request
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Imaging & Spectroscopy

Price on request
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Clinical and Anatomic Pathology

Price on request
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Project Management

Price on request
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Microscopy

Price on request
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  • Positive review for Immunohistochemistry (IHC):

    October 2015

    "Definitely will be using the IHC Core services with Luis Chiriboga's team again! Great communication throughout the process. Quality control was excellent and IHC looked great. Good turnaround time. Thanks again."

  • Positive review for Immunohistochemistry (IHC):

    January 2014

    "Great job!"

  • Positive review for Immunohistochemistry (IHC):

    January 2014

    "Very thorough work and nicely communicative!"

Immunohistochemistry (IHC) Core has not received any endorsements.