Dr Dimitri Scholz directs the UCD Conway Imaging Core where the focus is on providing contemporary imaging technologies and know-how to researchers in the wider scientific community; both in academia and industry. The consolidated instrumentation suite spans all aspects of light and electron microscopy as well as sample preparation and image processing and analysis facilities. The Core team use their technical expertise to assist in the widest possible range of applications for microscopy, in advancing the development of new applications and indeed new imaging technologies.

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Bright-Field Microscopy
Starting at $10.00 per hour

Applications
Research topics include oncology, dermatology, cardiology, biology of worms, insects and zebrafish as well as nano-biology. We can discuss your requirements for the imaging of native, stained or immunolabelled cells or tissue sections, time lapse imaging of live cells and Z-stack acquisition for 3D... Show more »

Applications
Research topics include oncology, dermatology, cardiology, biology of worms, insects and zebrafish as well as nano-biology. We can discuss your requirements for the imaging of native, stained or immunolabelled cells or tissue sections, time lapse imaging of live cells and Z-stack acquisition for 3D microscopy.


Instrumentation:
Nikon 80i with all top-optics and gadgets

Branches:
- Bright field
- Dark field
- Phase contrast
- Polarized light
- Differential interference contrast (DIC)


Expertise & Services:
- Image acquisition
- Image analysis
- Training in
a) sample preparation
b) imaging: transmission, fluorescent, confocal and live cell microscopy
c) image analysis

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Confocal Microscopy
Starting at $40.00 per hour

We got a great Zeiss LSM 510 Meta microscope.
3D reconstraction in Imaris
Deconvolution with Autoquant
We prowide the comprehensive training as well as the full service.

Laser lines:
351 nm
364 nm
488 nm
514 nm
543 nm
633 nm

We got a great Zeiss LSM 510 Meta microscope.
3D reconstraction in Imaris
Deconvolution with Autoquant
We prowide the comprehensive training as well as the full service.

Laser lines:
351 nm
364 nm
488 nm
514 nm
543 nm
633 nm

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Dark-Field Microscopy
Starting at $10.00 per hour

Best for water bugs

Best for water bugs

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DIC Microscopy
Differential interference contrast microscopy
Starting at $10.00 per hour

Best for non-stained cells (e.g. live) and tissue sections,
also in combination with the fluorescence.

Best for non-stained cells (e.g. live) and tissue sections,
also in combination with the fluorescence.

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Whole Slide Imaging
Price on request

This facility provides a slide scanning system that allows users to digitise microscopy slides, review and manage the images locally or remotely via the internet. The Aperio ScanScope XT system can scan entire glass slides at 20x or 40x magnification to create seamless, true-colour digital slide images in minutes. With capacity... Show more »

This facility provides a slide scanning system that allows users to digitise microscopy slides, review and manage the images locally or remotely via the internet. The Aperio ScanScope XT system can scan entire glass slides at 20x or 40x magnification to create seamless, true-colour digital slide images in minutes. With capacity for 120 slides, the system is suitable for full-face or tissue microarray slides. The main advantages of this type of image analysis is the inherent objectivity and the facility to perform batch analysis.


Instrumentation:
- ScanScope XT high throughput scanning system (Aperio Technologies)
- ImageScope image viewer with annotation and region extraction tools
- Spectrum, online slide management system with remote access
- Tissue microarray segmentation tool, TMALab
- Analysis toolbox for cell and area quantification of staining and intensity
- Server network for secure storage and hosting of digital slides


About Spectrum

The Spectrum platform provides a comprehensive slide management system where digital slides can be organised by sample, project or experiment. The slide viewer, ImageScope, is fully equipped with annotation and region extraction tools for highlighting and analysing features of interest within an image. Spectrum is also equipped with a tissue microarray (TMA) segmentation tool enabling identification and analysis of individual TMA cores. Spectrum’s comprehensive image analysis algorithm library provides automated whole slide or region analysis capabilities. Slide analysis is objective without potential errors associated with manual scoring and analysis. All data is saved securely in your Spectrum account, which can be accessed anywhere.


Services:
The digital pathology facility provides a range of technical services and expertise in this area which include:
- Advice on project design and specific requirements
- Training courses on slide scanning and digital slide analysis
- Assisted sessions on any aspect of digital pathology
- Slide scanning service
- Results analysis

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Fluorescence-Based Microscopy
Starting at $10.00 per hour

For fluorescently labeled cells and tissue sections:
- Epi-fluorescence, including Z-stack acquisition followed by deconvolution
- Laser confocal (single pinhole)
- Spinning disc confocal, including 2D and 3D montages
- Fluorescence resonance energy transfer (FRET)
- Fluorescence-lifetime imaging microscopy (FLIM)
-... Show more »

For fluorescently labeled cells and tissue sections:
- Epi-fluorescence, including Z-stack acquisition followed by deconvolution
- Laser confocal (single pinhole)
- Spinning disc confocal, including 2D and 3D montages
- Fluorescence resonance energy transfer (FRET)
- Fluorescence-lifetime imaging microscopy (FLIM)
- Fluorescence recovery after photobleaching (FRAP)
- Photoactivation
- Total internal reflection fluorescence (TIRF)

  • Training in
    a) sample preparation, including the immunolabeling
    b) imaging: transmission, fluorescent, confocal and live cell microscopy
    c) image analysis

Applications:
Research topics include oncology, dermatology, cardiology, biology of worms, insects and zebrafish as well as nano-biology. We can discuss your requirements for the imaging of native, stained or immunolabelled cells or tissue sections, time lapse imaging of live cells and Z-stack acquisition for 3D microscopy.

« Show less
Image Analysis
Price on request

Deconvolution
Volume reconstruction
Segmentation
Measurements
Statistics

Deconvolution
Volume reconstruction
Segmentation
Measurements
Statistics

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Live Cell Imaging
Starting at $15.00 per hour

We strongly recommend to study live cells if possible, for death is not what you want to study!

We offer the largest live cell imaging facility in Ireland: 5 top-ranged microscopes priced 150,000$ - 600,000$ each, capable to image cells in close-to-natural environment for up to 72 hours.

We run experiments in
- time lapse... Show more »

We strongly recommend to study live cells if possible, for death is not what you want to study!

We offer the largest live cell imaging facility in Ireland: 5 top-ranged microscopes priced 150,000$ - 600,000$ each, capable to image cells in close-to-natural environment for up to 72 hours.

We run experiments in
- time lapse
- Z-stack time lapse (4D)
- Multi-positioning time lapse (4D)
- Multi-positioning time lapse in Z-stack (5D)

Illumination options:
- Transmission light
- epi-Fluorescent light
- Laser confocal
- TIRF

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Phase Contrast Microscopy
Starting at $10.00 per hour

Best for non-stained cells (e.g. live) and tissue sections,
also in combination with the fluorescence.

Best for non-stained cells (e.g. live) and tissue sections,
also in combination with the fluorescence.

« Show less
Polarized Light Microscopy
Starting at $10.00 per hour

Best for biological and synthetical fibers and crystals

Best for biological and synthetical fibers and crystals

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Scanning Electron Microscopy (SEM)
Scanning Electron Microscopy services
Price on request

Image resolution in light microscopy is limited by the wavelength of light and is incapable of resolving structures of less than 200 nm. However, the resolution of electron microscopy limited by biological sample preparation goes beyond 1 nm.

Scanning Electron Microscopy (SEM) is used to investigate fine structure on surfaces... Show more »

Image resolution in light microscopy is limited by the wavelength of light and is incapable of resolving structures of less than 200 nm. However, the resolution of electron microscopy limited by biological sample preparation goes beyond 1 nm.

Scanning Electron Microscopy (SEM) is used to investigate fine structure on surfaces of biological and non-biological objects.


Applications:
Research topics include detection of viruses for human patients, inflammation, oncology, cardiovascular biology, morphology of zebrafish retina, bacteriology, food sciences, polymer films for biosensors, nano-particle toxicity in vitro and artificial joints.


Instrumentation:
- Three transmission electron microscopes (TEM)
- One scanning electron micropscope (SEM)
- Three ultramicrotomes, including one suitable for cryo-ultramicrotomy
- Fume hood, oven for EPON embedding and other TEM sample preparation instruments
- Critical point dryer, gold/carbon coater and other EM sample preparation instruments
- Light microscopes for EM sample preparation
- One scanning electron micropscope (SEM)

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Spinning Disk Confocal Microscopy
Starting at $15.00 per hour

Best for live cell imaging!
We run the microscope 8h a day but could find some slot for you.
Laser lines:
405 nm
445 nm
488 nm
515 nm
560 nm
595 nm

Best for live cell imaging!
We run the microscope 8h a day but could find some slot for you.
Laser lines:
405 nm
445 nm
488 nm
515 nm
560 nm
595 nm

« Show less
Transmission Electron Microscopy (TEM)
Transmission Electron Microscopy services
Price on request

Image resolution in light microscopy is limited by the wavelength of light and is incapable of resolving structures of less than 200 nm. However, the resolution of electron microscopy limited by biological sample preparation goes beyond 1 nm.

Transmission Electron Microscopy (TEM) is used to investigate ultrastructure of thin... Show more »

Image resolution in light microscopy is limited by the wavelength of light and is incapable of resolving structures of less than 200 nm. However, the resolution of electron microscopy limited by biological sample preparation goes beyond 1 nm.

Transmission Electron Microscopy (TEM) is used to investigate ultrastructure of thin samples (limited by the penetration of electron beam): flat cells, nano-particles, biological tissues embedded into polymers etc.


Applications:
Research topics include detection of viruses for human patients, inflammation, oncology, cardiovascular biology, morphology of zebrafish retina, bacteriology, food sciences, polymer films for biosensors, nano-particle toxicity in vitro and artificial joints.


Instrumentation:
- Three transmission electron microscopes (TEM)
- Three ultramicrotomes, including one suitable for cryo-ultramicrotomy
- Fume hood, oven for EPON embedding and other TEM sample preparation instruments
- Light microscopes for EM sample preparation

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Imaging & Spectroscopy
Price on request
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Microscopy
Price on request
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Electron Microscopy
Price on request
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Optical Microscopy
Price on request
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Transmitted Light Microscopy
Price on request
Request a quote for more information about this service.
Computational Modeling
Price on request
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Data Services
Price on request
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