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Automated Image Analysis
Price on request

IQbots

“IQbots” are the engines that power the ImageQuantify.com analysis and management workflow — tools
for common or custom preclinical imaging assays that deliver validated, comprehensive analytics that are
objective, reproducible, and accessible. Within every IQbot results package, researchers receive
complete... Show more »

IQbots

“IQbots” are the engines that power the ImageQuantify.com analysis and management workflow — tools
for common or custom preclinical imaging assays that deliver validated, comprehensive analytics that are
objective, reproducible, and accessible. Within every IQbot results package, researchers receive
complete data (i.e., quantitative excel spreadsheets, image analysis overlays, usable file formats), image
analysis validation certificate, and reference materials (e.g., a video walkthrough of output data, relevant
references from scientific literature, materials/methods section(s), and material to support GLP
compliance).

Credit Pricing

Our clients typically apply pre-paid credit bundles to image processing on a per-image basis. As such,
you are purchasing credits directly, rather than the analyses. Credits may be applied to any IQbot
available via ImageQuantify.com, and never depreciate nor expire (and also are not eligible for refund).
Via Science Exchange, we will allow clients to specifically pay for a certain analysis set. However, pricing
will be consistent with credit purchases made directly on our site. Costs will be calculated based on the
Following credit package prices (note: larger quantities of credits are discounted on a per-credit basis).

100 Credits: $175.00 USD
1,000 Credits: $1,450.00 USD
10,000 Credits: $10,500.00 USD
100,000 Credits: $45,000.00 USD

IQbots at a Glance

IQbot Version Research Area(s) Processing Time Expectation Acquisition Hardware Techniques, Dyes, Stains, & Proteins Sample Context
Tube Formation Analysis IQbot 1.0.0 Angiogenesis, Cancer Research Less than 4 Minutes Per Image Microscope Phase Contrast In-Vitro
Mouse Fundus Lesion Analysis IQbot 1.0.0 Angiogenesis, Diabetes, Drug Toxicity & Metabolism, Genetics, & Ophthalmology Less than 2 Minutes Per Image Funduscope or Ophthalmoscope -
Heart Muscle Fiber Analysis (Reticulin Stain) IQbot 1.0.0 Cardiovascular, Drug Toxicity & Metabolism, & Common Tools Less than 2 Minutes Per Image Wide-Field, Optical Microscope in Bright-Field (Transmission) Mode Using a Color Mosaic CCD or Monochrome CCD with RGB Filter Reticulin Stain In Tissue
Skeletal Muscle Fiber Analysis (Reticulin Stain) 1.0.0 Arthritis & Osteoarthritis, Drug Toxicity & Metabolism, Orthopedics, & Common Less than 2 Minutes Per Image Wide-Field, Optical Microscope in Bright-Field (Transmission) Mode Using a Color Mosaic CCD or Monochrome CCD with RGB Filter Reticulin Stain In Tissue
DAB Stain Analysis IQbot 1.0.0 Angiogenesis, Arthritis & Osteoarthritis, Cancer Research, Cardiovascular, Dermatology, Diabetes, Drug Toxicity & Metabolism, Genetics, Infectious Disease & Bacterial Adhesion, Neurology, Ophthalmology, Orthopedics, Reproductivity, & Common Tools Less than 2 Minutes Per Image Wide-Field, Optical Microscope in Bright-Field (Transmission) Mode Using a Color Mosaic CCD or Monochrome CCD with RGB Filter 3,3'-Diaminobenzidine (DAB), Horesradish Peroxidase (HRP), Hematoxylin Counterstain, Immunohistochemistry, Chromogen, Brown Oxidation Reaction In Tissue

IQbots in Detail


TUBE FORMATION ANALYSIS IQBOT

for examples and complete details, visit https://www.imagequantify.com/bots/botdetail/11

Analysis Overview

Endothelial cells, when provided with a 3D extracellular matrix substrate and appropriately supplemented
growth media, will self-organize into a network of capillary-like "tubes." This in-vitro assay is commonly
used to assess the ability of compounds to stimulate or inhibit angiogenesis. To perform the assay, the
endothelial cells are usually seeded (subconfluently) in multi-well plates filled with matrigel (extracellular
matrix). While not absolutely necessary (phase-contrast imaging is sufficient although often corrupted by
uneven lighting due to the meniscus formed in each well), endothelial cell membranes may be labeled
with fluorescent DiI dyes added directly to each well. Each well is subsequently imaged using a high
resolution, wide-field microscope using both phase-contrast and fluorescence modes. Images are
acquired while raster scanning across each well to form a single, high-resolution mosaic (i.e., multi-tile)
image. To analyze a given image, the IQbot applies a series of custom morphological and spectral filters
to the mosaic, allowing the endothelial cell network to be segmented and skeletonized for nodal and
branching analyses. In doing so, the IQbot is able to report specific area, lumen, node, and branch
characteristics of the network.

Details

IQbot ID: IQBOT-0001
Version: 1.0.0
Research Area(s): Angiogenesis, Cancer Research
Processing Time Expectation: Less than 4 Minutes Per Image
Acquisition Hardware: Microscope
Techniques, Dyes, Stains, & Proteins: Phase Contrast
Sample Context: In-Vitro

Output Parameters

Two versions of this IQbot are offered at two different price points, depending on the extent of your
analysis needs. Both offer the same output result documentation and image overlays. The ‘Basic’
analysis is priced at 3 credits per input image (see below for credit pricing details) and reports the
following quantitative metrics for each image in the results spreadsheet.

Vessel Area
Total Node Count
3-Branch Node Count
4-Branch Node Count
5+-Branch Node Count
Skeletal Length
Mean Node Thickness
Standard Deviation of Node Thickness
Branch Count
Mean Branch Thickness
Standard Deviation of Branch Thickness
Lumen Count
Mean Lumen Area

The ‘Advanced’ analysis tier (4 credits/image) reports all of those output parameters listed above, as well
as:

Endpoint Count
Mean Node-to-Node Length
Mean Endpoint-to-Node Length


MOUSE FUNDUS LESION ANALYSIS IQBOT

for examples and complete details, visit https://www.imagequantify.com/bots/botdetail/7

Analysis Overview

The Mouse Fundus Lesion Analysis IQbot segments objects (lesions) on images of a mouse retina
acquired using a fundus camera. A lesion is defined as any object that deviates from the general
surrounding retinal color profile. Such deviations are generally brighter and vary in hue. These lesions
can result from a number of pathologies, such as diabetic retinopathy, dry AMD, and diseases that
generate focal regions of depigmentation. In order to remove any contribution of the optic nerve and
vessels to the lesion count and area, the IQbot also segments these features prior to isolating, pseudo-
coloring, and analyzing lesions. Analysis outputs include individual and total lesion areas, lesion locations
(x,y-coordinates), the optic nerve centroid, and pseudo-colored overlays that provide segmentation
visualization of the optic nerve, vessels, and lesions.

Details

IQbot ID: IQBOT-0016
Version: 1.0.0
Research Area(s): Angiogenesis, Diabetes, Drug Toxicity & Metabolism, Genetics, & Ophthalmology
Processing Time Expectation: Less than 2 Minutes Per Image
Acquisition Hardware: Funduscope or Ophthalmoscope

Output Parameters

Two versions of this IQbot are offered at two different price points, depending on the extent of your
analysis needs. Both offer the same output result documentation and image overlays. The ‘Basic’
analysis is priced at 3 credits per input image (see below for credit pricing details) and reports the
following quantitative metrics for each image in the results spreadsheet.

Total Lesion Count
Mean Lesion Area
Standard Deviation of Lesion Area
Minimum Lesion Area
Maximum Lesion Area
Total Lesion Area
Lesion Area

The ‘Advanced’ analysis tier (4 credits/image) reports all of those output parameters listed above, as well
as:

Nerve Centroid X
Nerve Centroid Y
Lesion Centroid X
Lesion Centroid Y


HEART MUSCLE FIBER ANALYSIS (RETICULIN STAIN) IQBOT

for examples and complete details, visit https://www.imagequantify.com/bots/botdetail/5

Analysis Overview

The Heart Muscle Fiber Analysis IQbot analyzes muscle fiber content and morphology in histological
cross-sections of cardiac muscle stained for reticulin, a connective tissue fiber composed of type III
collagen. A number of metabolic and cardiovascular disorders can affect muscle fiber density, size, and
shape. Unfortunately, such phenotypic effects can be subtle, necessitating the quantification of
morphological parameters for as many muscle fibers as possible within a given image/cross-section for a
large cohort of samples — an exhaustive process if performed manually. Fortunately, the Heart Muscle
Fiber Analysis (Reticulin Stain) IQbot was specifically designed to rapidly and comprehensively segment
muscle fibers (in cross-section) within an image, extracting fibril number, area, aspect-ratio or elongation,
roundness or roughness, perimeter, and diameter. These can be presented as averages or, using an
advanced version of the IQbot, can be reported for each and every fibril segmented. Additionally,
segmented fibrils are pseudo-colored red and superimposed upon each image analyzed to enable
verification of the results and segmentation algorithm.

Details

IQbot ID: IQBOT-0012
Version: 1.0.0
Research Area(s): Cardiovascular, Drug Toxicity & Metabolism, & Common Tools
Processing Time Expectation: Less than 2 Minutes Per Image
Acquisition Hardware: Wide-Field, Optical Microscope in Bright-Field (Transmission) Mode Using a
Color Mosaic CCD or Monochrome CCD with RGB Filter
Techniques, Dyes, Stains, & Proteins: Reticulin Stain
Sample Context: In Tissue

Output Parameters

Two versions of this IQbot are offered at two different price points, depending on the extent of your
analysis needs. Both offer the same output result documentation and image overlays. The ‘Basic’
analysis is priced at 3 credits per input image (see below for credit pricing details) and reports the
following quantitative metrics for each image in the results spreadsheet.

Total Fibrils
Mean Area
Mean Aspect Ratio
Mean Roundness
Mean Diameter
Mean Perimeter

The ‘Advanced’ analysis tier (4 credits/image) reports all of those output parameters listed above, as well
as the following per-fibril quantities:

Area
Aspect Ratio
Roundness
Diameter
Perimeter


SKELETAL MUSCLE FIBER ANALYSIS (RETICULIN STAIN) IQBOT

for examples and complete details, visit https://www.imagequantify.com/bots/botdetail/22

Analysis Overview

The Skeletal Muscle Fiber Analysis IQbot analyzes muscle fiber content and morphology in histological
cross-sections of skeletal muscle stained for reticulin, a connective tissue fiber composed of type III
collagen. A number of metabolic and connective tissue disorders can affect muscle fiber density, size,
and shape. Unfortunately, such phenotypic effects can be subtle, necessitating the quantification of
morphological parameters for as many muscle fibers as possible within a given image or cross-section for
a large cohort of samples — an exhaustive process if performed manually. Fortunately, the Skeletal
Muscle Fiber Analysis IQbot was specifically designed to rapidly and comprehensively segment muscle
fibers (in cross-section) within an image, extracting fibril number, area, aspect-ratio or elongation,
roundness or roughness, perimeter, and diameter. These can be presented as averages or, using an
advanced version of the IQbot, can be reported for each and every fibril segmented. Additionally,
segmented fibrils are pseudo-colored red and superimposed upon each image analyzed to enable
verification of the results and segmentation algorithm.

Details

IQbot ID: IQBOT-0011
Version: 1.0.0
Research Area(s): Arthritis & Osteoarthritis, Drug Toxicity & Metabolism, Orthopedics, & Common
Tools
Processing Time Expectation: Less than 2 Minutes Per Image
Acquisition Hardware: Wide-Field, Optical Microscope in Bright-Field (Transmission) Mode Using a
Color Mosaic CCD or Monochrome CCD with RGB Filter
Techniques, Dyes, Stains, & Proteins: Reticulin Stain
Sample Context: In Tissue

Output Parameters

Two versions of this IQbot are offered at two different price points, depending on the extent of your
analysis needs. Both offer the same output result documentation and image overlays. The ‘Basic’
analysis is priced at 3 credits per input image (see below for credit pricing details) and reports the
following quantitative metrics for each image in the results spreadsheet.

Total Fibrils
Mean Area
Mean Aspect Ratio
Mean Roundness
Mean Diameter
Mean Perimeter

The ‘Advanced’ analysis tier (4 credits/image) reports all of those output parameters listed above, as well
as the following per-fibril quantities:

Area
Aspect Ratio
Roundness
Diameter
Perimeter


DAB STAIN ANALYSIS IQBOT

for examples and complete details, visit https://www.imagequantify.com/bots/botdetail/4

Analysis Overview

Since the brown-colored DAB stain is an oxidative byproduct of a specific protein bound to an enzyme
(i.e., HRP or horseradish peroxidase), it is agnostic to the structure and location of the target protein and
serves only as a reporter. Therefore, this IQbot's output parameters may represent nuclear staining (if the
DAB target is localized to nuclei) or regional staining (if the DAB target is extracellular and clustered).
These output parameters include total area of DAB staining and counts of the distinct regions of DAB
within the image. Additionally, with the aid of the counterstain, the area of tissue in the image is analyzed
to enable normalization of the DAB staining content. Lastly, in addition to the quantitative metrics, a
pseudo-colored image is generated for each image analyzed by the IQbot in which the segmented DAB
staining is pseudo-colored green.

Details

IQbot ID: IQBOT-0004
Version: 1.0.0
Research Area(s): Angiogenesis, Arthritis & Osteoarthritis, Cancer Research, Cardiovascular,
Dermatology, Diabetes, Drug Toxicity & Metabolism, Genetics, Infectious Disease & Bacterial Adhesion,
Neurology, Ophthalmology, Orthopedics, Reproductivity, & Common Tools
Processing Time Expectation: Less than 2 Minutes Per Image
Acquisition Hardware: Wide-Field, Optical Microscope in Bright-Field (Transmission) Mode Using a
Color Mosaic CCD or Monochrome CCD with RGB Filter
Techniques, Dyes, Stains, & Proteins: 3,3'-Diaminobenzidine (DAB), Horesradish Peroxidase (HRP),
Hematoxylin Counterstain, Immunohistochemistry, Chromogen, Brown Oxidation Reaction
Sample Context: In Tissue

Output Parameters

Total Tissue Area
Positive Nuclei or Region Count
Positive Nuclei or Region Area

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