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iFyber

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Ithaca, New York, US

About iFyber

Founded: 2009 Type: Privately Held Size: 11-50 employees

iFyber provides custom in vitro preclinical CRO services to companies and extend your R&D team by solving problems creatively and collaboratively. iFyber was founded on the principle that problems in healthcare are best resolved by focusing on the interface between... Show more »

iFyber provides custom in vitro preclinical CRO services to companies and extend your R&D team by solving problems creatively and collaboratively. iFyber was founded on the principle that problems in healthcare are best resolved by focusing on the interface between chemistry, biology and materials science.  In addition to biopharmaceuticals, we serve the wound care, infection control, orthopedics and regenerative medicine industries.  Our primary areas of expertise include biofilm models, biomaterial characterization, and custom method development.

Think of iFyber as:

  • Consultants with labs to back-up paper studies with experimental results
  • A testing services company eager to tailor methods to our clients’ needs
  • An academic lab working according to industrial product development schedules
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Our Services (8)


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Biofilm Susceptibility Testing

Price on request

Biofilms pose a major clinical threat. Current estimates from the US National Institutes of Health suggest biofilms account for over 80% of all clinical infections. Given the relatively recent understanding of biofilms, antibiofilm efficacy testing is becoming an increasingly common addition to the screening profile of new... Show more »

Biofilms pose a major clinical threat. Current estimates from the US National Institutes of Health suggest biofilms account for over 80% of all clinical infections. Given the relatively recent understanding of biofilms, antibiofilm efficacy testing is becoming an increasingly common addition to the screening profile of new antimicrobial technologies. iFyber offers a wide array of biofilm models, each are customizable to a client's technology.

EX VIVO PORCINE DERMAL MODEL FOR MATURE BIOFILMS:  This custom assay is a robust model of a mature bacterial biofilm, which are a hallmark of many bacterial infections that occur in soft tissues. This model isolates the biofilm component of these infections through the growth of a mature biofilm within a natural porcine dermal matrix. The resulting biofilm (typically grown over 72 hours) exhibits high tolerance to many antibiotics and, importantly, incorporates a relevant substrate when assessing product efficacy in the presence of soft tissue. iFyber has extensive experience adapting this model to our clients needs to test for antimicrobial and
anti-biofilm activity against a model for mature biofilm exhibiting tolerance to high doses of antibiotics. In this model, tissue explants are created, wounded, and subsequently infected to enable high throughput screening using a number of assay formats (prevention, eradication, dispersal, synergy).

MINIMUM BIOFILM ERADICATION (MBEC) ASSAY:  This high throughput assay comes in a microwell plate format, enabling growth of 96 bacterial biofilms on polystyrene pegs that protrude down from the lid of the plate into the corresponding well. As the biofilms are attached to the removable peg lid, this assay is set up to allow rapid testing of a range of materials for antibiofilm efficacy. Quantification is achieved by recovering remaining bacteria from the peg lid and enumerating either by standard agar plate counts, or for high throughput purposes, by optical density. iFyber has extensive experience using this assay system to screen antibiofilm technologies against both single-and multi-species biofilms.

MICROWELL BIOFILM SUSCEPTIBILITY ASSAYS:  This 96-well microtitre plate-based assay involves culturing bacterial biofilms in the wells of the plate, then exposing the formed biofilms to the test agent for a pre-defined time. The total remaining biofilm after exposure to the antimicrobial material is typically quantified by fluorescence methods using the nucleic acid stain, SYTO-9, or colorimetrically using Crystal Violet —a visible Gram stain that labels all components of a biofilm.

AN ARTIFICIAL SPUTUM IN VITRO MODEL FOR EVALUATION OF ANTIBIOTICS FOR TREATMENT OF CYSTIC FIBROSIS:  iFyber has implemented an in vitro model based on Artificial Sputum Medium (ASM) that mimics P. aeruginosa growth in the CF lung. ASM reflects the nutritional content of CF sputum, with high levels of mucin, DNA, and amino acids, and P. aeruginosa has been shown to form mucoid microcolonies within the ASM closely resembling those found in the CF lung. Furthermore, this model is amenable to high-throughput screening,
allowing assessment of multiple antibiotics and a range of concentrations simultaneously to provide a comparison of antibiotic efficacy against different species and strains of bacteria.

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Infectious Disease Multidrug resistant (MDR) infections Mixed Infection MRSA infection Staphylococcus aureus infection Cystic fibrosis Wound healing Infectious Disease colony-forming unit (CFU) assay in vitro testing Biology Drug Discovery Antibiotic Development Microbiology Microbial Isolates Bacteria Show 16 more tags Show less

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Minimal Biofilm Eradication Concentration (MBEC) Assay

Price on request

This high throughput assay comes in a microwell plate format, enabling growth of 96 bacterial biofilms on polystyrene pegs that protrude down from the lid of the plate into the corresponding well. As the biofilms are attached to the removable peg lid, this assay is set up to allow rapid testing of a range of materials for... Show more »

This high throughput assay comes in a microwell plate format, enabling growth of 96 bacterial biofilms on polystyrene pegs that protrude down from the lid of the plate into the corresponding well. As the biofilms are attached to the removable peg lid, this assay is set up to allow rapid testing of a range of materials for antibiofilm efficacy. Quantification is achieved by recovering remaining bacteria from the peg lid and enumerating either by standard agar plate counts, or for high throughput purposes, by optical density. iFyber has extensive experience using this assay system to screen antibiofilm technologies against both single-and multi-species biofilms.

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Infectious Disease Multidrug resistant (MDR) infections MRSA infection Staphylococcus aureus infection Wound healing Infectious Disease colony-forming unit (CFU) assay in vitro testing Biology Drug Discovery Antibiotic Development Microbiology Microbial Isolates Bacteria Show 14 more tags Show less

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Ex Vivo Models and Assays

Price on request

iFyber has adopted an ex vivo porcine dermal model of mature biofilm as a bridge between standard in vitro antimicrobial and anti-biofilm assays and in vivo animal studies. The model is suitable for evaluation of both biofilm prevention and eradication, and is useful for assessing the efficacy of a variety of products, from... Show more »

iFyber has adopted an ex vivo porcine dermal model of mature biofilm as a bridge between standard in vitro antimicrobial and anti-biofilm assays and in vivo animal studies. The model is suitable for evaluation of both biofilm prevention and eradication, and is useful for assessing the efficacy of a variety of products, from antibiotics to medical devices. Information generated with the model is more relevant to the developer than traditional in vitro studies—biofilms formed on the soft tissue dermal matrix more closely represent an actual wound bed compared to a rigid
polystyrene matrix. Animal models, on the other hand, are costly and not amenable to screening or optimization of dosing, formulation, and dressing format parameters.

This model, developed in the laboratory of Gregory Schultz at the University of Florida, uses sterilized pig skin explants with “wounds” in the dermis that support growth of mature biofilms within a wound-relevant matrix. Explants can be treated with a variety of dressings before or after inoculation and growth of a mature biofilm. Success is evaluated through recovery and enumeration of the bacteria remaining on the explant, and coupled with confocal microscopy for visual confirmation of the effects against biofilm, if desired. The ex vivo model is not intended for
understanding the immunological aspects of wounds in living systems; however, is does isolate the biofilm component of a chronic wound, which is important for conducting controlled studies on biofilm management. This screening method is commercially available for pre-clinical studies in chronic wounds, acute bacterial and skin suture infections (ABSSSI), chronic acne, and fungal skin infections.

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Infectious Disease Multidrug resistant (MDR) infections MRSA infection Staphylococcus aureus infection Wound healing Infectious Disease colony-forming unit (CFU) assay in vitro testing Biology Drug Discovery Antibiotic Development Microbiology Microbial Isolates Bacteria Aging Show 15 more tags Show less

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Antimicrobial Susceptibility Testing

Price on request

iFyber provides a range of assays that can be tailored to test a material in a way that is relevant to its proposed end use. We also actively develop novel antimicrobial assay systems based on our client’s specific needs. Relevant materials for testing include new antibiotics/biocides, coatings, nanomaterials, wound... Show more »

iFyber provides a range of assays that can be tailored to test a material in a way that is relevant to its proposed end use. We also actively develop novel antimicrobial assay systems based on our client’s specific needs. Relevant materials for testing include new antibiotics/biocides, coatings, nanomaterials, wound dressings, coated natural tissue matrices, and coated medical devices.

DYNAMIC CONTACT ASSAY:  The dynamic contact assay is used to determine the efficacy of
antimicrobial agents that are bonded to a substrate surface, and are therefore not expected to leach during conditions of normal use. Through a process of continuous agitation, the test is designed to maximize the contact between the non-leachable antimicrobial agent, and the bacterial species against which it is being tested.

ZONE OF INHIBITION (ZOI) ASSAY:  The zone of inhibition (ZOI) assay is designed to assess the antimicrobial activity of leachable agents that have been applied onto the surface of, or incorporated into, solid substrates. The antimicrobial material is placed on top of a bacterial ‘lawn’ that has been grown on nutrient agar. After a defined incubation period, the agar plates are examined: If the leachable agent is biocidal against the test bacteria, a clear region (i.e., zone of inhibition) will be apparent around the test material. Quantification is achieved by measuring the diameter of the zone.

MINIMUM INHIBITORY CONCENTRATION (MIC) ASSAY:  The MIC assay is a high-throughput approach for determining bacterial susceptibility to soluble antimicrobial material. It is used to determine the lowest concentration of an antimicrobial agent that inhibits bacterial growth. The typical experimental approach involves exposing the bacterial species of choice to a dilution range of the antimicrobial test agent, then quantifying bacterial growth. A standard quantification approach is turbidity measurement (optical density) using a spectrophotometer.

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Infectious Disease Multidrug resistant (MDR) infections Bacteremia MRSA infection Sepsis Staphylococcus aureus infection Wound healing Infectious Disease Bioburden Tesitng colony-forming unit (CFU) assay in vitro testing Biology Drug Discovery Antibiotic Development Microbiology Microbial Isolates Bacteria Show 17 more tags Show less

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Antimicrobial Efficacy Testing

Price on request

iFyber provides a range of assays that can be tailored to test a material in a way that is relevant to its proposed end use. We also actively develop novel antimicrobial assay systems based on our client’s specific needs. Relevant materials for testing include new antibiotics/biocides, coatings, nanomaterials, wound... Show more »

iFyber provides a range of assays that can be tailored to test a material in a way that is relevant to its proposed end use. We also actively develop novel antimicrobial assay systems based on our client’s specific needs. Relevant materials for testing include new antibiotics/biocides, coatings, nanomaterials, wound dressings, coated natural tissue matrices, and coated medical devices.

DYNAMIC CONTACT ASSAY:  The dynamic contact assay is used to determine the efficacy of
antimicrobial agents that are bonded to a substrate surface, and are therefore not expected to leach during conditions of normal use. Through a process of continuous agitation, the test is designed to maximize the contact between the non-leachable antimicrobial agent, and the bacterial species against which it is being tested.

ZONE OF INHIBITION (ZOI) ASSAY:  The zone of inhibition (ZOI) assay is designed to assess the antimicrobial activity of leachable agents that have been applied onto the surface of, or incorporated into, solid substrates. The antimicrobial material is placed on top of a bacterial ‘lawn’ that has been grown on nutrient agar. After a defined incubation period, the agar plates are examined: If the leachable agent is biocidal against the test bacteria, a clear region (i.e., zone of inhibition) will be apparent around the test material. Quantification is achieved by measuring the diameter of the zone.

MINIMUM INHIBITORY CONCENTRATION (MIC) ASSAY:  The MIC assay is a high-throughput approach for determining bacterial susceptibility to soluble antimicrobial material. It is used to determine the lowest concentration of an antimicrobial agent that inhibits bacterial growth. The typical experimental approach involves exposing the bacterial species of choice to a dilution range of the antimicrobial test agent, then quantifying bacterial growth. A standard quantification approach is turbidity measurement (optical density) using a spectrophotometer.

Show less
Multidrug resistant (MDR) infections Bacteremia MRSA infection Sepsis Staphylococcus aureus infection Wound healing Infectious Disease Biology Drug Discovery Antibiotic Development Microbiology Microbial Isolates Bacteria Show 13 more tags Show less

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Analytical Method Development

Price on request

iFyber focuses on custom method development.  We have access to a wide variety of instruments and methods and focus on the interface between chemistry, materials science, and biology.

  • Chemistry Methods

iFyber focuses on custom method development.  We have access to a wide variety of instruments and methods and focus on the interface between chemistry, materials science, and biology.

  • Chemistry Methods
    •  Spectroscopy
      • NMR
      • FT-IR
      • Raman & SERS
      • EPR
      • UV-Vis
    • Mass Spec
      • ICP
      • DART
      • LC-MS
  • Materials Methods
    • Electron microscopy
      • TEM
      • SEM
      • EDX
      • X-ray Diffraction
      • UV-Vis
    • Thermogravimetric analysis
      • Dynamic light scattering
      • Differential scanning calorimetry
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Biocompatibility Testing

Price on request

We specialize in screening new technologies, investigational compounds, medical devices, wound dressings and the like) for compatibility issues with mammalian biological systems.
• MTT Assay
• LIVE/DEAD Staining
• TUNEL Assay
• BrdU Assay
• Neutral Red Uptake
• Trypan Blue Exclusion

We specialize in screening new technologies, investigational compounds, medical devices, wound dressings and the like) for compatibility issues with mammalian biological systems.
• MTT Assay
• LIVE/DEAD Staining
• TUNEL Assay
• BrdU Assay
• Neutral Red Uptake
• Trypan Blue Exclusion

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Raman Spectroscopy

Price on request

iFyber's deep background in spectroscopic methods, in particular Surface Enhanced Raman Spectroscopy (SERS), is useful for extremely sensitive detection of molecules in the lab or in the field.  We have a comprehensive skill-set in sensor molecule design, synthesis, method development, and testing... Show more »

iFyber's deep background in spectroscopic methods, in particular Surface Enhanced Raman Spectroscopy (SERS), is useful for extremely sensitive detection of molecules in the lab or in the field.  We have a comprehensive skill-set in sensor molecule design, synthesis, method development, and testing capability.

Applications include detection and identification of:

  • Illicit drugs
  • Explosives
  • Pesticides
  • Contaminants in the food and beverage industry
  • Biomarkers for medical diagnostics in spectroscopic, electroanalytical or chromatographic methods.

iFyber has developed its own proprietary porous SERS membrane (SER-DM) and has designed and demonstrated its use with chemical probes that provide and additional level of specificity for chemical detection.

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FileType/PDF Created with Sketch. App Notes CF_small.pdf

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FileType/PDF Created with Sketch. Ciprofloxacin Biofilm App Note.pdf

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FileType/PDF Created with Sketch. Efficacy Data Packages-compressed.pdf

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FileType/PDF Created with Sketch. iFyber ex vivo app note.pdf

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FileType/PDF Created with Sketch. ifyber_antibacterial_efficacy.pdf

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FileType/PDF Created with Sketch. ifyber_antimicrobial_drug_dev.pdf

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FileType/PDF Created with Sketch. ifyber_biocompatibility.pdf

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FileType/PDF Created with Sketch. ifyber_biofilms.pdf

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