Default

Icosagen Technologies Inc.

No Reviews Yet
No Completed Orders
San Francisco, California, US

About Icosagen Technologies Inc.

Icosagen is a research-driven contract research provider for biopharmaceutical and biotechnology industries that deftly adapts to market forces and current trends, and is always guided by the needs of its customers.

Icosagen offers custom-tailored services for recombinant protein production in mammalian... Show more »

Icosagen is a research-driven contract research provider for biopharmaceutical and biotechnology industries that deftly adapts to market forces and current trends, and is always guided by the needs of its customers.

Icosagen offers custom-tailored services for recombinant protein production in mammalian cells, and antibody development. By using patented state-of-the-art technologies, we deliver high quality products to the biopharmaceutical and diagnostic companies. Our company's quality control and assurance system is ISO9001:2008 certified.

Show less

Our Services (16)


ic

Recombinant Antibody Production

Price on request

Icosagen offers a broad range of tailored services for the development and production of recombinant antibodies, including complex antibody formats.

Our customers benefit from our cost effective QMCF Expression Technology that is based on the replication, and stable maintenance of the expression vectors in CHO cells, in... Show more »

Icosagen offers a broad range of tailored services for the development and production of recombinant antibodies, including complex antibody formats.

Our customers benefit from our cost effective QMCF Expression Technology that is based on the replication, and stable maintenance of the expression vectors in CHO cells, in conjunction with specifically optimized culture conditions for the recombinant antibody production.

We can start either from antibody (or VH/VL) sequence information, hybridoma cells, or cDNA, and we deliver high quality material (purity >98%, endotoxin levels <0,1 EU/mg) together with elaborated project report containing detailed QC data and analysis.

Recombinant antibody production services includes the possibility to change antibody subtype or host specificity, and to generate various types of antibodies, such as human IgG1, IgG2, IgG4, mouse IgG1, IgG2a, IgG2b, IgG3, rat IgG1, IgG2a, rabbit IgG, chicken IgY, chimeric antibodies, antibody fragments, single chain molecules, bispecific antibodies (bi- scFV-Fc, DVD-Ig, Crossmabs), etc.

There are two options for recombinant antibody production by using QMCF Technology:

  • Enhanced transient production of recombinant antibodies is used to deliver up to hundreds of milligrams of purified antibody within 3-4 weeks starting from vector cloning.
  • Stable episomal production is used to scale up the production volumes up to 40 liters cost effectively, and to deliver multiple gram quantities of purified antibody. In this case, also episomal production cell bank can be generated, which allows to omit transfection completely when repetitive and constant supply of the same antibody is required with consistent reproducibility.

Transient production includes:

  • cDNA optimization and cloning into transient pQMCF expression vector
  • Chemical transfection of the QMCF/CHOEBNALT85 cell line and expression control 48 hours after transfection
  • Optional culture expansion (up to 2.5 fold) and recombinant antibody production
  • Harvesting and purification
  • Product analysis and characterization
  • Weekly updates, and detailed reports

Stable production, including selection and cell bank generation:

  • cDNA optimization and cloning into pQMCF expression vector
  • Electroporation of the QMCF/CHOEBNALT85 cell line and expression control 48 hours after transfection
  • G418 selection and generation of production cell bank (stable episomal pool)
  • Culture expansion to the final production volume and recombinant antibody production
  • Harvesting and purification
  • Product analysis and characterization
  • Weekly updates, and detailed reports
Show less

ic

Protein Production

Price on request

Recombinant protein production service

Icosagen provides recombinant protein production services using proprietary CHO-based QMCF Technology in serum-free conditions. Customer benefits are a short production time and cost-efficiency.

We offer two options for protein production:

  • Enhanced transient expression of recombinant... Show more »

Recombinant protein production service

Icosagen provides recombinant protein production services using proprietary CHO-based QMCF Technology in serum-free conditions. Customer benefits are a short production time and cost-efficiency.

We offer two options for protein production:

  • Enhanced transient expression of recombinant proteins using high copy number transient pQMCF-T expression vectors. This option is fast, and a good fit for a small-scale pilot study to test the best expression conditions for your protein of interest.
  • Production using stable episomal production pools for large-scale production using pQMCF expression vectors that are stably maintained in proliferating CHO cells. This option is scalable and suitable for the cost effective production in up to 40 liter scale, and to produce protein of interest in up to gram-scale.

Transient production of recombinant proteins includes:

  • cDNA optimization and cloning into transient pQMCF-T expression vector
  • Chemical transfection of QMCF/CHOEBNALT85 cell line and expression/secretion control 48 hours after transfection
  • Optional culture expansion (up to 2.5 folds) and recombinant protein production
  • Harvesting and protein purification
  • Product analysis and characterization
  • Weekly updates, and detailed reports

Stable production including selection and cell bank generation:

  • cDNA optimization and cloning into pQMCF expression vector
  • Electroporation of QMCF/CHOEBNALT85 cell line and expression/secretion control 48 hours after transfection
  • G418 selection and generation of production cell bank (stable episomal pool)
  • Culture expansion to the final production volume and recombinant protein production
  • Harvesting and protein purification
  • Product analysis and characterization
  • Weekly updates, and detailed reports
Show less

ic

Membrane Protein Expression

Price on request

Production of VLP-s pseudotyped with membrane proteins

Expression of viral envelope or capsid proteins results in the self-assembly of virus-like particles (VLPs) that provide a great platform for the production of functional membrane proteins with native folding. This kind of pseudotyped VLPs could be used for different... Show more »

Production of VLP-s pseudotyped with membrane proteins

Expression of viral envelope or capsid proteins results in the self-assembly of virus-like particles (VLPs) that provide a great platform for the production of functional membrane proteins with native folding. This kind of pseudotyped VLPs could be used for different approaches, e.g. vaccine development, investigation of receptor functions, screening of antibodies, for immunization and also for purification of different membrane proteins.

Icosagen produces VLPs pseudotyped with membrane proteins, such as ion-channels, receptors, viral glycoproteins, etc. For the formation of VLPs, we use retroviral (HIV-1 or MLV) and filoviral (ebola) proteins. VLPs could be produced in 293 or CHO-based QMCF cell lines.

Our advantages

  • Fast and efficient production of VLPs pseudotyped with different receptors, ion-channels, viral glycoproteins, etc.
  • Human-specific posttranslational modification pattern using 293-based cell line
Show less

ic

Protein Purification

Price on request
Request a quote for more information about this service.

ic

Polyclonal Antibody Development

Price on request

Icosagen provides a full service production of polyclonal antibodies in rabbit or chicken hosts. For immunization, KLH (or other carrier) conjugated peptides, recombinant proteins and other available immunogenes could be used. We recommend using recombinant proteins for immunization due to the stronger immune response and larger... Show more »

Icosagen provides a full service production of polyclonal antibodies in rabbit or chicken hosts. For immunization, KLH (or other carrier) conjugated peptides, recombinant proteins and other available immunogenes could be used. We recommend using recombinant proteins for immunization due to the stronger immune response and larger number of epitopes available when compared with peptide immunization.

Polyclonal antibody production service includes:

  • Immunogene (recombinant protein) production or peptide conjugation with various carriers
  • Test animal immunization
  • Testing of antiserum
  • Antibody affinity purification
  • Antibody characterization/antigene recognition by Western blot testing

In the case of chicken (IgY) antibody generation, 2-3 immunizations will be performed within 30 days, and the required number of eggs will be collected. Antibody IgY fraction is extracted from egg yolk, followed by affinity purification.

For generation of rabbit polyclonal antibodies, 3-4 immunizations within 45 to 90 days are needed to develop a good immune response. Thereafter, serum is collected and antibodies are affinity purified.

For immunization, 0.5 - 3 mg of immunogenes per one test animal is needed. Additionally, approximately 5 mg of protein is needed for affinity purification.

Show less

ic

Monoclonal Antibody (mAb) Production

Price on request

Monoclonal antibody development service by mouse hybridoma technology

This service includes immunization of mice with immunogenes, following by hybridoma generation, screening and subcloning, antibody production in hybridoma cell culture, purification and characterization.

We develop antibodies against KLH (or other carrier)... Show more »

Monoclonal antibody development service by mouse hybridoma technology

This service includes immunization of mice with immunogenes, following by hybridoma generation, screening and subcloning, antibody production in hybridoma cell culture, purification and characterization.

We develop antibodies against KLH (or other carrier) conjugated peptides, recombinant proteins, and other available immunogenes. We recommend the use of recombinant proteins due to stronger immune response and availability of larger number of epitopes when compared with peptide immunization.

Show less

ic

Monoclonal Antibody (mAb) Development

Price on request

Icosagen offers monoclonal antibody development from chicken and rabbits by using our proprietary HybriFree technology that is based on the VH/VL cloning from B-cells.

Background

Development of monoclonal antibodies (MAbs) using HybriFree Technology is attractive for generation of immunomolecules from species other than mice.... Show more »

Icosagen offers monoclonal antibody development from chicken and rabbits by using our proprietary HybriFree technology that is based on the VH/VL cloning from B-cells.

Background

Development of monoclonal antibodies (MAbs) using HybriFree Technology is attractive for generation of immunomolecules from species other than mice. There are several technologies described for the development of recombinant monoclonal antibodies including phage display, single B-cell cloning, proteomics, NGS (Next Generation Sequencing) based etc. However, these all have drawbacks: using bacterial or yeast expression system in phage display, selected MAbs may not be functional; problems with robustness (single B-cell manipulations and/or sterile cultivation of B-cells) may occur; or expensive devices or patented technologies are needed.

We have worked out HybriFree Technology for the development of monoclonal antibodies from different species such as chicken and rabbits. It can also be used for cloning antibodies from existing hybridomas.

The process contains following steps:

  1. Isolation of PBMCs from the animal spleen. Enrichment of B-cells expressing antigen-specific antibodies from the PBMC population
  • RNA isolation, generation of cDNA library of VH/VL combinations and construction of full IgG or ScFv-Fc expression library
  • High-throughput screening of the VH/VL combinatory library expressed in CHO cells. Identification of high-affinity VH/VL combinations by ELISA/Octet K2 followed by sequencing and reconstruction if necessary.
  • Production of immunomolecules (full antibodies, fragments, chimeric molecules, etc) by QMCF expresssion technology in mammalian (CHO) cells

The HybriFree Technology has several advantages:

  • Fast- sequence information of the antigen-binding VH/VL pair can be obtained in 6-7 weeks starting from the immunization, and purified antibodies can be delivered in as fast as 8-9 weeks
  • Cost efficient- antigen-recognizing enrichment of B-cell population reduces significantly size of antibody library
  • Manufacturability- B-cells are used for the in vivo development, and CHO cells are used for the screening of the full IgG or ScFv-Fc libraries
  • Universal - antibodies can be reconstructed into different subtypes, origins and structures
  • QMCF mammalian expression system can be used to produce antigens, as well as milligrams to grams amounts of developed antibodies
Show less

ic

Medical Devices & Diagnostics

Price on request
Request a quote for more information about this service.

ic

Biology

Price on request
Request a quote for more information about this service.

ic

Antibody Services

Price on request
Request a quote for more information about this service.

ic

Biochemical Assays

Price on request
Request a quote for more information about this service.

ic

Biochemical Assay Kits

Price on request
Request a quote for more information about this service.

ic

Companion Diagnostic Development

Price on request
Request a quote for more information about this service.

ic

Antigen Binding Fragments (Fab)

Price on request
Request a quote for more information about this service.

ic

Biochemistry & Molecular Biology

Price on request
Request a quote for more information about this service.

ic

Protein Services

Price on request
Request a quote for more information about this service.

Not finding what you're looking for?

Get info on this provider's capabilities without requesting a quote.
Missing Avatar

Andrew Wilfong

President

Icosagen Technologies Inc. has not received any reviews.

Icosagen Technologies Inc. has not received any endorsements.