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Hyperbaric Biomedical Research Laboratory (HBRL)

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Tampa, Florida, US

About Hyperbaric Biomedical Research Laboratory (HBRL)

The USF Hyperbaric Biomedical Research Laboratory is a collaborative research facility housed in the Department of Molecular Pharmacology and Physiology in the laboratories of Dr. Jay B. Dean.

The HBRL maintains several hyperbaric/hypobaric pressure chambers containing equipment used to measure cellular... Show more »

The USF Hyperbaric Biomedical Research Laboratory is a collaborative research facility housed in the Department of Molecular Pharmacology and Physiology in the laboratories of Dr. Jay B. Dean.

The HBRL maintains several hyperbaric/hypobaric pressure chambers containing equipment used to measure cellular function in real time via electrophysiology, polarography, fluorescence microscopy and atomic force microscopy during experimental perturbations of barometric pressure and gas partial pressures.

The mission of the USF-HBRL is to identify the molecular and cellular mechanisms involved in the body’s response to artificial atmospheres and altered pressure environments, including low barometric pressures (hypobaria), normal sea level pressure (normobaria) and high barometric pressures (hyperbaria). Altered pressure environments perturb various cellular processes at the molecular level due to the effects of pressure per se, gas partial pressure alone, and/or the production of secondary reaction productions such as O2-induced free radicals or CO2-induced protons. Altered pressure environments are routinely encountered in hyperbaric medicine (hyperbaric oxygen therapy), underwater diving (hyperbaric gases) and space exploration (hypobaric gases).

To study cellular processes under these conditions, Dr. Dean and his colleagues at USF, Dr. Enrico Camporesi (Chairman, USF Anesthesiology) and Dr. Dominic D’Agostino (Molecular Pharmacology and Physiology), have assembled six hyperbaric/hypobaric pressure chambers for in vitro and in vivo biomedical research.
In addition, the laboratory manager, Carol Landon, Dr Dean's Postdoctoral Scholars Dr. Raffaele Pilla and Dr. Heather Held, Graduate Student Geoffrey Ciarlone, and Dr. D'Agostino's Gradutate Student Angela Poff are working on a number of animal (rats and mice) and human, in vivo and in vitro projects related to hyperbaric oxygen exposure.

The design, development and implementation of these novel research tools have been funded primarily through equipment grants from the Department of Defense and Office of Naval Research (ONR), Undersea Medicine Program. Ongoing basic research by the USF-HBRL team is currently supported by the ONR, NIH and USF College of Medicine.

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Our Services (16)


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Cell Electrophysiology

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In vitro cell and tissue electrophysiology 1- 11 atmospheres absolute (ATA)

Normobaric Electrophysiology

  1. The first brain slice electrophysiology and imaging station includes the following items: 2 amplifiers (Axopatch-1D patch-clamp amplifier; and Dagan PC-One patch/whole cell clamp); 2 Burleigh microdrives; Nikon FN1... Show more »

In vitro cell and tissue electrophysiology 1- 11 atmospheres absolute (ATA)

Normobaric Electrophysiology

  1. The first brain slice electrophysiology and imaging station includes the following items: 2 amplifiers (Axopatch-1D patch-clamp amplifier; and Dagan PC-One patch/whole cell clamp); 2 Burleigh microdrives; Nikon FN1 fixed-stage microscope equipped with: DIC optics and IR video microscopy, fluorescence attachment and Roper CoolSnap camera; brain slice recording chamber; vibration isolation table; window discriminators & rate meters; 6 channel timer/stimulator with SIU; 2 oscilloscopes; 2 channel DC amplifier/filter; Digidata 1322A/pCLAMP data acquisition system with computer; water bath; temperature controller; and fiber optic illuminator.

  2. The second brain slice electrophysiology and imaging station includes: Nikon TE2000 inverted microscope, which will be mated to the Atto Bioscience CARV I spinning disk confocal cell imaging microscope (that is removed from Nautilus and replaced with the C1si laser scanning confocal system). Important pieces of equipment for fluorescence microscopy include: Roper Cool Snap Digital Camera; Xenon light source; light guide; Lambda 10-3 shutter controller and interface; Universal Imaging Metamorph/Metafluor 6.0 Software for data collection and analysis.

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Atomic Force Microscopy (AFM) Services

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In vitro cell and tissue preparations atomic force microscopy (AFM) 1- 6.8 atmospheres absolute (ATA)

In vitro cell and tissue preparations atomic force microscopy (AFM) 1- 6.8 atmospheres absolute (ATA)

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Fluorescence-Based Microscopy

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In vitro cell and tissue real time fluorescence microscopy 0.3-67.3 atmospheres absolute (ATA)

In vitro cell and tissue real time fluorescence microscopy 1 - 11 atmospheres absolute (ATA)

In vitro cell and tissue real time fluorescence microscopy 0.3-67.3 atmospheres absolute (ATA)

In vitro cell and tissue real time fluorescence microscopy 1 - 11 atmospheres absolute (ATA)

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Animal Hyperbaric Medicine

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Animal exposure to hyperbaric gases 1-3.4 atmospheres absolute ATA

Hyperbaric gas research chambers for animal use:
Model 17-48 Hyperbaric Animal Research Chamber (Reimers Systems, Inc.). The hypobaric/hyperbaric chamber has a MWP= 6.8 ATA (= 190 fsw) and measures 17.25 × 49.00 inches.

Sechrist chambers, Model... Show more »

Animal exposure to hyperbaric gases 1-3.4 atmospheres absolute ATA

Hyperbaric gas research chambers for animal use:
Model 17-48 Hyperbaric Animal Research Chamber (Reimers Systems, Inc.). The hypobaric/hyperbaric chamber has a MWP= 6.8 ATA (= 190 fsw) and measures 17.25 × 49.00 inches.

Sechrist chambers, Model 1300-B—2 x hyperbaric chambers (MWP= 3 ATA= 66 fsw each) used for hyperoxic oxidative preconditioning (2-2.5 ATA O2) or control “preconditioning” (normobaric air & normoxic heliox, 2-2.5 ATA total pressure) of awake rats. Rats are then studied using “4” (Paul Bert) or chambers “1-3” (for in vitro experiments in brain tissue slices harvested from preconditioned rats)

Armstrong (brain slice incubation)—hyperbaric chamber with a MWP= 90 psig (= 6.1 ATA= 202 fsw) outfitted with a brain slice chamber for tissue slice incubation at hyperbaric pressures using O2 or various combinations of O2, CO2, N2 or He.

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Ex vivo Electrophysiology

Price on request

In vitro cell and tissue electrophysiology 1- 11 atmospheres absolute (ATA)

Normobaric Electrophysiology

  1. The first brain slice electrophysiology and imaging station includes the following items: 2 amplifiers (Axopatch-1D patch-clamp amplifier; and Dagan PC-One patch/whole cell clamp); 2 Burleigh microdrives; Nikon FN1... Show more »

In vitro cell and tissue electrophysiology 1- 11 atmospheres absolute (ATA)

Normobaric Electrophysiology

  1. The first brain slice electrophysiology and imaging station includes the following items: 2 amplifiers (Axopatch-1D patch-clamp amplifier; and Dagan PC-One patch/whole cell clamp); 2 Burleigh microdrives; Nikon FN1 fixed-stage microscope equipped with: DIC optics and IR video microscopy, fluorescence attachment and Roper CoolSnap camera; brain slice recording chamber; vibration isolation table; window discriminators & rate meters; 6 channel timer/stimulator with SIU; 2 oscilloscopes; 2 channel DC amplifier/filter; Digidata 1322A/pCLAMP data acquisition system with computer; water bath; temperature controller; and fiber optic illuminator.

  2. The second brain slice electrophysiology and imaging station includes: Nikon TE2000 inverted microscope, which will be mated to the Atto Bioscience CARV I spinning disk confocal cell imaging microscope (that is removed from Nautilus and replaced with the C1si laser scanning confocal system). Important pieces of equipment for fluorescence microscopy include: Roper Cool Snap Digital Camera; Xenon light source; light guide; Lambda 10-3 shutter controller and interface; Universal Imaging Metamorph/Metafluor 6.0 Software for data collection and analysis.

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Mammalian Cell Culture

Price on request

The cell culture facility (~ 180 ft2) contains the following equipment utilized in maintaining two cell lines: 1) Thermo Scientific NAPCO Series 8000 Water Jacketed CO2 Incubator, 2) Forma Scientific Class II, Type A BioSafe Cabinet, 3) Precision Digital Water Bath, 4) Nikon Eclipse TS100 Inverted Microscope. In addition, we... Show more »

The cell culture facility (~ 180 ft2) contains the following equipment utilized in maintaining two cell lines: 1) Thermo Scientific NAPCO Series 8000 Water Jacketed CO2 Incubator, 2) Forma Scientific Class II, Type A BioSafe Cabinet, 3) Precision Digital Water Bath, 4) Nikon Eclipse TS100 Inverted Microscope. In addition, we utilized the following equipment in a common equipment room: 1) Beckman GS6R Tabletop Centrifuge, 2) Locator 8 Plus Liquid Nitrogen Dewer and 3) AMSCO Scientific Autoclave.

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Patch Clamp Recording

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Imaging & Spectroscopy

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Animal Model in vivo Analyses

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Optical Microscopy

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Biology

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Animal Models and Studies

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Cells and Tissues

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Animal Physiology Analyses

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Scanning Probe Microscopy

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Microscopy

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