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Histopathology and Tissue Shared Resource

Washington, District of Columbia

The HTSR offers a full complement of histology services through our combined Human Tissue Bank, histology, and IHC departments. The Human Tissue Bank supports tissue procurement and the retrieval of fresh frozen and archival paraffin blocks. The Histology laboratory provides processing, embedding and microtomy services both for fresh frozen and fixed tissues. The Histology lab also provides numerous routine and non-routine histological stains. The Immunohistochemistry (IHC) laboratory supports colorimetric and fluorescent IHC staining for established antibodies as well as the development of protocols for new antibodies. The IHC lab also supports TUNEL labeling and novel labeling techniques.

    Cryo-embedding
    $11.25 USD
    per Sample

    Embedding of fresh or fixed samples into OCT embedded blocks.

    Embedding of fresh or fixed samples into OCT embedded blocks.

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    Cryosectioning
    $6.50 USD
    per Slide

    Sectioning of unstained frozen tissue samples.

    Sectioning of unstained frozen tissue samples.

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    Fluorescence microscopy
    $50.00 USD
    per Hour

    HistoRx automated quantitative analysis (AQUA) staining for quantitative fluorescent immunostaining

    HistoRx automated quantitative analysis (AQUA) staining for quantitative fluorescent immunostaining

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    H&E stain
    Hematoxylin and eosin stain
    $6.50 USD
    per Slide

    Includes sectioning and H&E staining of paraffin embedded sample

    Sectioning and H&E Staining of frozen tissue blocks.
    Price: $8.00 USD per Sample

    Deliverables:
    - stained slides

    Includes sectioning and H&E staining of paraffin embedded sample

    Sectioning and H&E Staining of frozen tissue blocks.
    Price: $8.00 USD per Sample

    Deliverables:
    - stained slides

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    Immunofluorescence
    Price on request
    Please contact Histopathology and Tissue Shared Resource for more information regarding this service.
    Immunohistochemistry
    $19.00 USD
    per Slide

    Immunological staining of tissue sections. Development of novel staining procedures.

    The laboratory provides the following services:
    - 2 DAKO Autostainers for high throughput immunohistochemical staining.
    - Protocol development using new antibodies from commercial vendors or custom antibodies.
    - Optimization... Show more »

    Immunological staining of tissue sections. Development of novel staining procedures.

    The laboratory provides the following services:
    - 2 DAKO Autostainers for high throughput immunohistochemical staining.
    - Protocol development using new antibodies from commercial vendors or custom antibodies.
    - Optimization of staining intensity and specificity.
    - Assisting with interpretation.
    - Protein expression within tissues by immunohistochemistry and immunofluorescence.
    - HistoRx for quantitative fluorescent immunostaining.

    Fees:
    IHC Development $12.00
    Immunostain $19.00
    Antibody CBCB
    TUNEL $32.00
    AQUA $27.00

    Deliverables:
    - stained slides

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    Paraffin Embedding
    $7.50 USD
    per Sample

    Processing and sectioning. Routine and non-routine histology stains.

    Processing and sectioning. Routine and non-routine histology stains.

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    Sectioning of paraffin embedded tissue
    $5.25 USD
    per Slide

    Sectioning of paraffin embedded tissue to produce unstained slides.

    Sectioning of paraffin embedded tissue to produce unstained slides.

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    Special slide staining
    $11.00 USD
    per Sample

    Staining of slides using dye based histopathology. Stains include but are not limited to:
    Masson's Trichrome
    Mast Cell Staining (Chloroacetate esterase)
    Periodic Acid Schiff's (PAS)
    Toluidine Blue
    Nissl Stain
    Gram Stain
    Sirius Red

    Deliverables:
    - stained slides

    Staining of slides using dye based histopathology. Stains include but are not limited to:
    Masson's Trichrome
    Mast Cell Staining (Chloroacetate esterase)
    Periodic Acid Schiff's (PAS)
    Toluidine Blue
    Nissl Stain
    Gram Stain
    Sirius Red

    Deliverables:
    - stained slides

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    TUNEL
    Terminal deoxynucleotidyl transferase dUTP nick end labeling assay
    $32.00 USD
    per Sample

    TUNEL is used to detect apoptotic cells in tissue sections.

    TUNEL is used to detect apoptotic cells in tissue sections.

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Sonja Dean gave a positive review for Histology Methods:

December 12, 2011
Deborah was prompt and professional in all interactions and the quality of work was exceptional.

Sonja Dean gave a positive review for Histology Methods:

October 04, 2011
Deborah was very reliable, professional, and communicative. Our lab was very happy with the quality, turn around time, and price for service. I certainly recommend Deborah and the services of the Lombardi Institute to anyone.
March 19, 2014
Deb was great to work with! She was fast, responsive, and very communicative. She even did a bit of extra work above and beyond the requirements of the project, and the data she returned was very clean and clear. I'm looking forward to working with Deb on more projects!

Joeli Brinkman gave a positive review for Immunohistochemistry:

April 22, 2013
The staining looks great!

Joeli Brinkman gave a positive review for Immunohistochemistry:

March 27, 2013
Deborah provides excellent service!

Toby Ward gave a positive review for Immunohistochemistry:

August 06, 2012
fantastic service, very helpful and knowledgeable. Will definitely use again for immunohistochemistry.

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