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Histology Research Core Facility

Chapel Hill, North Carolina, US

With the advancement and popular emphasis in molecular techniques in today’s laboratory, anatomy-based research—often critical in defining research projects—is becoming a lost art. Many research laboratories no longer have the floor space nor can afford the specialized histology equipment and the personnel required to perform these complex procedures. Today, researchers frequently include expensive mouse models to support and complement their research projects. Consistent and reliable histology is critical in providing structural and quantitative data for these mouse/animal models. The Departmental Histology Facility offers a variety of services insuring that histological techniques are always available for investigators and their research project needs.

The Histology Facility of the Department of Cell and Molecular Physiology at UNC-Chapel Hill is housed in a 600-square foot research laboratory in the Glaxo... Show more »

With the advancement and popular emphasis in molecular techniques in today’s laboratory, anatomy-based research—often critical in defining research projects—is becoming a lost art. Many research laboratories no longer have the floor space nor can afford the specialized histology equipment and the personnel required to perform these complex procedures. Today, researchers frequently include expensive mouse models to support and complement their research projects. Consistent and reliable histology is critical in providing structural and quantitative data for these mouse/animal models. The Departmental Histology Facility offers a variety of services insuring that histological techniques are always available for investigators and their research project needs.

The Histology Facility of the Department of Cell and Molecular Physiology at UNC-Chapel Hill is housed in a 600-square foot research laboratory in the Glaxo Building, room 4. The facility is equipped with two Leica 1950 cryostats, a Cryojane Tape Transfer frozen sectioning system, two Leica 2235 rotary microtomes, microscopes and a Vibratome.

The Facility has the equipment and expertise to produce reliable high-quality sections from fixed and unfixed tissues, frozen and paraffin-embedded tissues. Furthermore we provide a unique serial interrupted sectioning technique. This technique allows analysis of the full length of 4mm vessels or tissues, collected on multiple slides with each slide containing serial sections representing a specific distance through the full length of that tissue. In addition, special stains and immunohistochemical assays are routinely performed in the facility.

Recent Publications

  • Cooley B, Funkhouser W, Monroe D, Ezzell A, Mann DM, Lin FC, Monahan PE, Stafford DW. Prophylactic efficacy of BeneFIX vs Alprolix in hemophilia B mice. Blood. 2016 Apr 22. pii: blood-2016-01-696104. [Epub ahead of print]
  • Cozzo AJ, Sundaram S, Ottavia Zattra O, Qin Y, Freemerman AJ, Essaid L, Darr DB, Montgomery SA, McNaughton KK, Ezzell JA, Galanko JA, Troester MA, Makowski L. cMET inhibitor crizotinib impairs angiogenesis and reduces tumor burden in the C3(1)-Tag model of basal-like breast cancer. Springer Plus Breast Cancer Collection, IN PRESS.
  • Corbin JM, Overcash RF, Wren JD, Coburn A, Tipton GJ, Ezzell JA, McNaughton KK, Fung KM, Kosanke SD, Ruiz-Echevarria MJ. Analysis of TMEFF2 allografts and transgenic mouse models reveals roles in prostate regeneration and cancer. Prostate. 2015 Sep 29. doi: 10.1002/pros.23103. [Epub ahead of print] PMID:26417683
  • Van Landeghem L, Santoro MA, Mah AT, Krebs AE, Dehmer JJ, McNaughton KK, Helmrath MA, Magness ST, Lund PK. IGF1 stimulates crypt expansion via differential activation of 2 intestinal stem cell populations. FASEB J. 2015 Apr 2. pii: fj.14-264010. [Epub ahead of print] PMID:25837582
  • Sundaram S, Freemerman AJ, Galanko JA, McNaughton KK, Bendt KM, Darr DB, Troester MA, Makowski L. Obesity-mediated regulation of HGF/c-Met is associated with reduced basal-like breast cancer latency in parous mice. PLoS One. 2014 Oct 29;9(10):e111394. doi: 10.1371/journal.pone.0111394. eCollection 2014. PMID: 25354395
  • Casbas-Hernandez P, Sun X, Roman-Perez E, D'arcy M, Sandhu R, Hishida A, McNaughton KK, Yang XR, Makowski L, Sherman ME, Figueroa JD, Troester MA. Tumor Intrinsic Subtype is Reflected in Cancer-Adjacent Tissue. Cancer Epidemiol Biomarkers Prev. 2014 Dec 2. pii: cebp.0934.2014. PMID:25465802
  • Sundaram S, Le TL, Essaid L, Freemerman AJ, Huang MJ, Galanko JA, McNaughton KK, Bendt KM, Darr DB, Troester MA, Makowski L. Weight Loss Reversed Obesity-Induced HGF/c-Met Pathway and Basal-Like Breast Cancer Progression. Front Oncol. 2014 Jul 8;4:175. doi: 10.3389/fonc.2014.00175. eCollection 2014. PMID:25072025
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Histology Research Core Facility has not listed any services.

Mallory's Trichrome Staining
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Alcian Blue Staining
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Neuronal Staining
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H&E Staining
Hematoxylin and eosin staining
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Immunostaining
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Immunofluorescence
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Our IF services include mouse-on-mouse capabilities, singe antigen labeling, multiple antigen labeling, heat induced epitope retrieval (HIER), providing positive and negative control slides and consultation.

Our IF services include mouse-on-mouse capabilities, singe antigen labeling, multiple antigen labeling, heat induced epitope retrieval (HIER), providing positive and negative control slides and consultation.

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Histological Staining
Price on request

We are proficient in a variety of special stains including but not limited to H&E, Masson's Trichome, Combined Masson's Elastin, Oil Red O, Picrosirius Red, PAS, Alcian Blue, CAS, Von Kossa, Phloxine/Tartrazine Alcian Blue, AZAN, CEV, Acid Fast Bacilli, Gomori Trichrome, Luxol Fast Blue, Nuclear Fast Red, Movats Pentachrome, Prussian Blue, Safranin O, Toluidine Blue and X-gal.

We are proficient in a variety of special stains including but not limited to H&E, Masson's Trichome, Combined Masson's Elastin, Oil Red O, Picrosirius Red, PAS, Alcian Blue, CAS, Von Kossa, Phloxine/Tartrazine Alcian Blue, AZAN, CEV, Acid Fast Bacilli, Gomori Trichrome, Luxol Fast Blue, Nuclear Fast Red, Movats Pentachrome, Prussian Blue, Safranin O, Toluidine Blue and X-gal.

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Tissue Sectioning
Price on request

The Facility has the equipment and expertise to produce reliable high-quality sections from fixed, unfixed, frozen and paraffin-embedded tissues. We specialize in customizing slide layouts to fit your needs. Furthermore we provide a unique serial interrupted sectioning technique. This technique allows efficient analysis of the... Show more »

The Facility has the equipment and expertise to produce reliable high-quality sections from fixed, unfixed, frozen and paraffin-embedded tissues. We specialize in customizing slide layouts to fit your needs. Furthermore we provide a unique serial interrupted sectioning technique. This technique allows efficient analysis of the full length of vessels or tissues. We also have the CryoJane Tape Transfer system for tissues that difficult to section such as fat.

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Immunohistochemistry (IHC)
Price on request

Our IHC services include enzyme based IHC, polymer based IHC, mouse-on-mouse capabilities, chromogenic detection, singe antigen labeling, double antigen labeling, heat induced epitope retrieval (HIER), free floating assays, providing positive and negative control slides and consultation.

Our IHC services include enzyme based IHC, polymer based IHC, mouse-on-mouse capabilities, chromogenic detection, singe antigen labeling, double antigen labeling, heat induced epitope retrieval (HIER), free floating assays, providing positive and negative control slides and consultation.

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Clinical Research
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Clinical Laboratory Services
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Clinical and Anatomic Pathology
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Common Histochemical Stains
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Biology
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Cells and Tissues
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Biospecimens
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Tissue Processing
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Biochemistry & Molecular Biology
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Protein Services
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Protein Expression Visualization
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2018-07-30 14:52:45 +0200

Net Promoter Score of 7 received for Tissue Sectioning.

Additional Ratings: satisfaction with deliverable: 7, satisfaction with timeliness: 7.
2018-05-15 16:07:46 +0200

Net Promoter Score of 10 received for Immunohistochemistry (IHC).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 10.
2018-04-17 22:39:04 +0200

Net Promoter Score of 8 received for Cells and Tissues.

2018-02-28 14:55:05 +0100

Net Promoter Score of 8 received for Histological Staining.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 8.
2018-01-12 21:08:29 +0100

Net Promoter Score of 5 received for Immunostaining.

Additional Ratings: satisfaction with deliverable: 5, satisfaction with timeliness: 5.

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