With the advancement and popular emphasis in molecular techniques in today’s laboratory, anatomy-based research—often critical in defining research projects—is becoming a lost art. Many research laboratories no longer have the floor space nor can afford the specialized histology equipment and the personnel required to perform these complex procedures. Today, researchers frequently include expensive mouse models to support and complement their research projects. Consistent and reliable histology is critical in providing structural and quantitative data for these mouse/animal models. The Departmental Histology Facility offers a variety of services insuring that histological techniques are always available for investigators and their research project needs.
The Histology Facility of the Department of Cell and Molecular Physiology at UNC-Chapel Hill is housed in a 600-square foot research laboratory in the Glaxo Building, room 4. The facility is equipped with two Leica 1950 cryostats, a Cryojane Tape Transfer frozen sectioning system, two Leica 2235 rotary microtomes, microscopes and a Vibratome.
The Facility has the equipment and expertise to produce reliable high-quality sections from fixed and unfixed tissues, frozen and paraffin-embedded tissues. Furthermore we provide a unique serial interrupted sectioning technique. This technique allows analysis of the full length of 4mm vessels or tissues, collected on multiple slides with each slide containing serial sections representing a specific distance through the full length of that tissue. In addition, special stains and immunohistochemical assays are routinely performed in the facility.
Our IF services include mouse-on-mouse capabilities, singe antigen labeling, multiple antigen labeling, heat induced epitope retrieval (HIER), providing positive and negative control slides and consultation.
We are proficient in a variety of special stains including but not limited to H&E, Masson's Trichome, Combined Masson's Elastin, Oil Red O, Picrosirius Red, PAS, Alcian Blue, CAS, Von Kossa, Phloxine/Tartrazine Alcian Blue, AZAN, CEV, Acid Fast Bacilli, Gomori Trichrome, Luxol Fast Blue, Nuclear Fast Red, Movats Pentachrome, Prussian Blue, Safranin O, Toluidine Blue and X-gal.
The Facility has the equipment and expertise to produce reliable high-quality sections from fixed, unfixed, frozen and paraffin-embedded tissues. We specialize in customizing slide layouts to fit your needs. Furthermore we provide a unique serial interrupted sectioning technique. This technique allows efficient analysis of the full length of vessels or tissues. We also have the CryoJane Tape Transfer system for tissues that difficult to section such as fat.
Our IHC services include enzyme based IHC, polymer based IHC, mouse-on-mouse capabilities, chromogenic detection, singe antigen labeling, double antigen labeling, heat induced epitope retrieval (HIER), free floating assays, providing positive and negative control slides and consultation.
Histology Research Core Facility has not received any endorsements.