1378341791163 805edf9a9623d2626baa7ca310a3b316

Histology Core Facility at Lake Nona

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Orlando, Florida, US

About Histology Core Facility at Lake Nona

The Histology Core at Lake Nona is a full-service shared resource facility that provides histological services to Sanford-Burnham researchers and investigators throughout the scientific community. The Core offers technical support, consultative, and interpretive pathology to all investigators. Tissue samples... Show more »

The Histology Core at Lake Nona is a full-service shared resource facility that provides histological services to Sanford-Burnham researchers and investigators throughout the scientific community. The Core offers technical support, consultative, and interpretive pathology to all investigators. Tissue samples are processed with the highest industry quality standards, using state-of-the-art instrumentation. The Core also assists with experiment design as well as the development and interpretation of tests and their results.

Sanford-Burnham’s Histology Core at Lake Nona is committed to collaborating with investigators and advancing research by carrying out critical tissue specimen validation and evaluation.

Equipment and Resources:
- Thermo Excelsior ES Tissue Processor
- Thermo Histocentre™ 3 Embedding Unit
- Thermo Cytospin 4
- Thermo Microm HM355S Microtome
- Thermo Printmate 450 Cassette Printer
- Thermo Microm HM550 Cryostat
- Thermo Microm HM650 V Vibratome with cooling unit
- Thermo Microm HM450 Sliding Microtome with freezing unit
- Leica IP S Slide Printer
- Leica RM2235 Microtome
- Leica 1900UV Cryostat
- Nikon Eclipse 80i Microscope with AR Elements imaging software
- Ventana Discovery XT
- Tissue-Tek® Prisma Automated Slide Stainer and Glas™ g2 Coverslipper
- Aperio ScanScope XT-Brightfield
- Aperio ScanScope FL-Fluorescence

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Our Services (14)


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Immunofluorescence

Starting at $92.00 per slide

Capabilities:
- preparation and mounting of both whole and sectioned tissue from fixed tissues
- preparation of both paraffin-embedded and frozen sections
- culture mammalian cells and prepare them for antibody staining: we're happy to work with any cells that are sent to us, however no infectioned cell line above BCL2
-... Show more »

Capabilities:
- preparation and mounting of both whole and sectioned tissue from fixed tissues
- preparation of both paraffin-embedded and frozen sections
- culture mammalian cells and prepare them for antibody staining: we're happy to work with any cells that are sent to us, however no infectioned cell line above BCL2
- culture non-mammalian (i.e. insect) cells (Protein Production Core: https://www.scienceexchange.com/labs/protein-production-analysis-core)
- imaging of live cells

Data analyses available:
- Fluorescence quantitation
- 3D reconstruction
- Bright field quantification

We do not:
- have access to a tissue bank
- have access to in-house FFPE tissues
- transfect cells with either viruses or chemicals

Equipment:
- Epifluorescence
- Stereoscope
- Confocal
- Upright
- Inverted

Objective lenses:
- 4x ,10x, 20x, 40x, 60x, 100x

Fluorescent channels and wavelengths:
- 4, anything from DAPI to far red


Antibody Titration and Testing (IF) (cost per antibody) - For profit companies $526.00 - Non-profit companies $270.00

Double labeling of two antibodies on the same slide (IF) (cost per slide) - For profit companies $184.10 - Non-profit companies $94.50

Immuno-histochemistry Staining (IF) (cost per slide) - For profit companies $92.05 - Non-profit companies $47.25

TUNEL Assay (IF (cost per slide) - For profit companies $92.05 - Non-profit companies $47.25

In Situ Hybridization (FISH or CISH) (cost per slide) - For profit companies $197.25 - Non-profit companies $101.25

Probe optimization for In Situ Hybrdization (FISH or CISH) (cost per probe) - For profit companies $789.00 - Non-profit companies $405.00

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Immunohistochemistry (IHC)

Starting at $47.25 per slide

Immuno-histochemistry Staining (cost per slide) - For profit companies $92.05 - Non-profit companies $47.25

Antibody Titration and Testing Per antibody (cost per antibody) - For profit companies $526.00 - Non-profit companies $270.00

Double labeling of two antibodies on the same slide (cost per slide) - For profit companies... Show more »

Immuno-histochemistry Staining (cost per slide) - For profit companies $92.05 - Non-profit companies $47.25

Antibody Titration and Testing Per antibody (cost per antibody) - For profit companies $526.00 - Non-profit companies $270.00

Double labeling of two antibodies on the same slide (cost per slide) - For profit companies $184.10 - Non-profit companies $94.50

TUNEL Assay (cost per slide) - For profit companies $92.05 - Non-profit companies $47.25

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VENTANA Discovery XT

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H&E Staining

Hematoxylin and eosin staining
Starting at $1.01 per slide

Per Batch - For profit companies $26.30 - Non-profit companies $13.50

[Batch = 1 to 20 Slides]

Per Batch - For profit companies $26.30 - Non-profit companies $13.50

[Batch = 1 to 20 Slides]

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Special Slide Staining

Starting at $26.30 per sample

Special Stains (PAS, Oil Red O, Trichrome....) (cost per slide) For profit companies $26.30 - Non-profit companies $13.50

Special stains
- Masson’s trichrome
- Oil Red O
- Modified Gomori’s trichrome for muscle sections
- Other stains upon request

Enzyme histochemistry stains
- SDH (succinic dehydrogenase) stain
- ATPase

Special Stains (PAS, Oil Red O, Trichrome....) (cost per slide) For profit companies $26.30 - Non-profit companies $13.50

Special stains
- Masson’s trichrome
- Oil Red O
- Modified Gomori’s trichrome for muscle sections
- Other stains upon request

Enzyme histochemistry stains
- SDH (succinic dehydrogenase) stain
- ATPase

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Clinical Laboratory Services

Price on request
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Clinical Research

Price on request
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Histological Staining

Price on request
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Biochemistry & Molecular Biology

Price on request
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Biology

Price on request
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Common Histochemical Stains

Price on request
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Protein Expression Visualization

Price on request
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Immunostaining

Price on request
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Protein Services

Price on request
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Clinical and Anatomic Pathology

Price on request
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