HistoBridge

Histopathology is the “gold standard” for the evaluation of cellular and tissue changes associated with a disease process or genetic defect. Routine sections are stained with hematoxylin and eosin, and a variety of other stains are commonly used to highlight various features or cell types.

Histobridge offers scientific/technical consultation, including:

• Protocols/Experimental Design
  
  • Preparation/review.
    
• In-Life Phase
  
  • Monitoring and data analysis/interpretation.
    
• Histopathology
  
  • Examination and interpretation of findings.
    
• Pathology Reports
  
  • Preparation/review.
    
• General Study Reports
  
  • Preparation/review.
    
• Peer Review (Independent Party Opinion)
  
  • Of all phases of studies.
    
• Quality Assurance/GLP Compliance
  
  • Of all phases of studies.
    
• Toxicology Summaries/Executive Summaries/Drug Resumes/Manuscripts
  
  • Preparation/review.
    
• Documents for Regulatory Submission (INDs, NDAs, PLAs, etc.)
  
  • Preparation/review.
    
• Client Representation
  
  • At regulatory agencies, contract research organizations, etc.

We currently provide high quality immunohistochemical staining:

• High quality IHC staining: Using standard protocols developed in-house or provided by the client.
  
  • Paraffin-embedded human or rodent tissue blocks or unstained slide
    
  • OCT-embedded human or rodent frozen tissue blocks or unstained slides
    
• Optimizing IHC protocol: we optimize IHC protocols for primary antibodies that are commercially available or supplied by the client.

We currently provide the following special stains:

• Masson's Trichrome: To demonstrate the differentiation between collagen and smooth muscle in tumors and identify increases in collagenous tissue in diseases.
  
• Oil Red O: To demonstrate neutral lipids in frozen tissue section; can only be performed on frozen tissue sections.
  
• PAS: A stain to detect polysaccharides, neutral mucosubstances & basement membranes.
  
• Picrosirius Red: To visualize collagen I and III fibers in addition to muscle in tissue sections. The PSR stain may be viewed using standard light microscopy or polarized light resulting in birefringence of the collagen fibers to distinguish between type I and type III.

We currently provide digital slide scanning and image analysis using ImageScope or Image J.